DOI: 10.1038/sj.embor.7400858. The supramolecular architecture of junctional microdomains in native lens membranes

The supramolecular architecture of junctional microdomains in native lens membranes

10.1038/sj.embor.7400858

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Gap junctions formed by connexons and thin junctions formed by lens‐specific aquaporin 0 (AQP0) mediate the tight packing of fibre cells necessary for lens transparency. Gap junctions conduct water, ions and metabolites between cells, whereas junctional AQP0 seems to be involved in cell adhesion. High‐resolution atomic force microscopy (AFM) showed the supramolecular organization of these proteins in native lens core membranes, in which AQP0 forms two‐dimensional arrays that are surrounded by densely packed gap junction channels. These junctional microdomains simultaneously provide adhesion and communication between fibre cells. The AFM topographs also showed that the extracellular loops of AQP0 in junctional microdomains adopt a conformation that closely resembles the structure of junctional AQP0, in which the water pore is thought to be closed. Finally, time‐lapse AFM imaging provided insights into AQP0 array formation. This first high‐resolution view of a multicomponent eukaryotic membrane shows how membrane proteins self‐assemble into functional microdomains.

Bibliography

Buzhynskyy, N., Hite, R. K., Walz, T., & Scheuring, S. (2006). The supramolecular architecture of junctional microdomains in native lens membranes. EMBO Reports, 8(1), 51â55. Portico.

Authors 4

Nikolay Buzhynskyy (first)
Richard K Hite (additional)
Thomas Walz (additional)
Simon Scheuring (additional)

References 28 Referenced 93

10.1007/s10540-005-2577-2
10.1016/0014-4835(75)90040-8
10.1103/PhysRevLett.56.930
10.1007/s002329900266
{'key': 'e_1_2_6_6_1', 'first-page': '118', 'article-title': 'Molecular solutions to mammalian lens transparency', 'volume': '16', 'author': 'Donaldson P', 'year': '2001', 'journal-title': 'News Physiol Sci'} / News Physiol Sci / Molecular solutions to mammalian lens transparency by Donaldson P (2001)
10.1016/j.molcel.2004.08.016
10.1016/S0022-2275(20)42242-4 / J Lipid Res / Lipid composition of lens plasma membrane fractions enriched in fiber junctions by Fleschner CR (1991)
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10.1016/S0006-3495(04)74280-0
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10.1016/j.jmb.2004.07.076
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{'key': 'e_1_2_6_25_1', 'first-page': '443', 'article-title': 'Covalent changes in MIP26K during aging of the human lens membrane', 'volume': '27', 'author': 'Takemoto L', 'year': '1986', 'journal-title': 'Invest Ophthalmol Vis Sci'} / Invest Ophthalmol Vis Sci / Covalent changes in MIP26K during aging of the human lens membrane by Takemoto L (1986)
10.1126/science.283.5405.1176
10.1074/jbc.M414377200
10.1083/jcb.93.1.175
10.1006/exer.2002.2041

Dates

Type	When
Created	18 years, 8 months ago (Nov. 24, 2006, 6:21 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:58 p.m.)
Indexed	3 weeks, 4 days ago (July 28, 2025, 2:27 a.m.)
Issued	18 years, 8 months ago (Nov. 24, 2006)
Published	18 years, 8 months ago (Nov. 24, 2006)
Published Online	18 years, 8 months ago (Nov. 24, 2006)
Published Print	18 years, 7 months ago (Jan. 1, 2007)

Funders 0

None

BibTeX

@article{Buzhynskyy_2006, title={The supramolecular architecture of junctional microdomains in native lens membranes}, volume={8}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/sj.embor.7400858}, DOI={10.1038/sj.embor.7400858}, number={1}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Buzhynskyy, Nikolay and Hite, Richard K and Walz, Thomas and Scheuring, Simon}, year={2006}, month=nov, pages={51–55} }

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