DOI: 10.1038/sj.embor.7400663. Structure and oligomeric state of human transcription factor TFIIE

Structure and oligomeric state of human transcription factor TFIIE

10.1038/sj.embor.7400663

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

The general RNA polymerase II transcription factor TFIIE, which is composed of two subunits, has essential roles in both transcription initiation and promoter escape. Electron microscopy analysis of negatively stained human TFIIE showed a large proportion of α/β heterodimers as well as a small proportion of tetramers. Analytical ultracentrifugation, chemical crosslinking, pulldown experiments and cryo‐electron microscopy confirmed that TFIIE is a α/β heterodimer in solution. Three‐dimensional envelopes of the α/β particles showed an elongated structure composed of three distinct modules. Finally, a model for the quaternary architecture of the complex is proposed that provides a structural framework to discuss the function of TFIIE in the context of RNA polymerase II transcription initiation.

Bibliography

Jawhari, A., Uhring, M., De Carlo, S., Crucifix, C., TocchiniâValentini, G., Moras, D., Schultz, P., & Poterszman, A. (2006). Structure and oligomeric state of human transcription factor TFIIE. EMBO Reports, 7(5), 500â505. Portico.

Authors 8

Anass Jawhari (first)
Muriel Uhring (additional)
Sacha De Carlo (additional)
Corinne Crucifix (additional)
Guiseppe Tocchini‐Valentini (additional)
Dino Moras (additional)
Patrick Schultz (additional)
Arnaud Poterszman (additional)

References 26 Referenced 22

10.1016/S0968-4328(97)00068-1
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10.1126/science.288.5470.1418
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10.1038/358641a0
10.1074/jbc.M307874200
10.1038/368160a0
10.1073/pnas.87.23.9163
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10.1074/jbc.273.31.19866
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10.1093/emboj/19.6.1346
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Dates

Type	When
Created	19 years, 5 months ago (March 17, 2006, 10:48 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:58 p.m.)
Indexed	1 year, 3 months ago (May 7, 2024, 4:14 p.m.)
Issued	19 years, 5 months ago (March 17, 2006)
Published	19 years, 5 months ago (March 17, 2006)
Published Online	19 years, 5 months ago (March 17, 2006)
Published Print	19 years, 3 months ago (May 1, 2006)

Funders 0

None

BibTeX

@article{Jawhari_2006, title={Structure and oligomeric state of human transcription factor TFIIE}, volume={7}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/sj.embor.7400663}, DOI={10.1038/sj.embor.7400663}, number={5}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Jawhari, Anass and Uhring, Muriel and De Carlo, Sacha and Crucifix, Corinne and Tocchini‐Valentini, Guiseppe and Moras, Dino and Schultz, Patrick and Poterszman, Arnaud}, year={2006}, month=mar, pages={500–505} }

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