DOI: 10.1038/sj.embor.7400248. Toll‐like receptor 2‐dependent bacterial sensing does not occur via peptidoglycan recognition

Toll‐like receptor 2‐dependent bacterial sensing does not occur via peptidoglycan recognition

10.1038/sj.embor.7400248

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Toll‐like receptor 2 (TLR2) has been shown to recognize several classes of pathogen‐associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have relied mainly on the use of commercial Staphylococcus aureus PG and have not addressed TLR2 recognition of other PG types. Using highly purified PGs from eight bacteria (Escherichia coli, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Helicobacter pylori, Bacillus subtilis, Listeria monocytogenes, Streptococcus pneumoniae and S. aureus), we show that these PGs are not sensed through TLR2, TLR2/1 or TLR2/6. PG sensing is lost after removal of lipoproteins or lipoteichoic acids (LTAs) from Gram‐negative and Gram‐positive cell walls, respectively. Accordingly, purified LTAs are sensed synergistically through TLR2/1. Finally, we show that elicited peritoneal murine macrophages do not produce tumour necrosis factor‐α or interleukin‐6 in response to purified PGs, suggesting that PG detection is more likely to occur intracellularly (through Nod1/Nod2) rather than from the extracellular compartment.

Bibliography

Travassos, L. H., Girardin, S. E., Philpott, D. J., Blanot, D., Nahori, M., Werts, C., & Boneca, I. G. (2004). Tollâlike receptor 2âdependent bacterial sensing does not occur via peptidoglycan recognition. EMBO Reports, 5(10), 1000â1006. Portico.

Authors 7

Leonardo H Travassos (first)
Stephen E Girardin (additional)
Dana J Philpott (additional)
Didier Blanot (additional)
Marie‐Anne Nahori (additional)
Catherine Werts (additional)
Ivo G Boneca (additional)

References 26 Referenced 371

Dates

Type	When
Created	20 years, 11 months ago (Sept. 10, 2004, 3:02 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:57 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 4:52 p.m.)
Issued	20 years, 10 months ago (Oct. 1, 2004)
Published	20 years, 10 months ago (Oct. 1, 2004)
Published Online	20 years, 10 months ago (Oct. 1, 2004)
Published Print	20 years, 10 months ago (Oct. 1, 2004)

Funders 0

None

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  "abstract": "<jats:p>Toll\u2010like receptor 2 (TLR2) has been shown to recognize several classes of pathogen\u2010associated molecular patterns including peptidoglycan (PG). However, studies linking PG with TLR2 recognition have relied mainly on the use of commercial <jats:italic>Staphylococcus aureus</jats:italic> PG and have not addressed TLR2 recognition of other PG types. Using highly purified PGs from eight bacteria (<jats:italic>Escherichia coli</jats:italic>, <jats:italic>Pseudomonas aeruginosa</jats:italic>, <jats:italic>Yersinia pseudotuberculosis</jats:italic>, <jats:italic>Helicobacter pylori</jats:italic>, <jats:italic>Bacillus subtilis</jats:italic>, <jats:italic>Listeria monocytogenes</jats:italic>, <jats:italic>Streptococcus pneumoniae</jats:italic> and <jats:italic>S. aureus</jats:italic>), we show that these PGs are not sensed through TLR2, TLR2/1 or TLR2/6. PG sensing is lost after removal of lipoproteins or lipoteichoic acids (LTAs) from Gram\u2010negative and Gram\u2010positive cell walls, respectively. Accordingly, purified LTAs are sensed synergistically through TLR2/1. Finally, we show that elicited peritoneal murine macrophages do not produce tumour necrosis factor\u2010\u03b1 or interleukin\u20106 in response to purified PGs, suggesting that PG detection is more likely to occur intracellularly (through Nod1/Nod2) rather than from the extracellular compartment.</jats:p>",
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