DOI: 10.1038/sj.embor.7400206. Helicase Motif III in SecA is essential for coupling preprotein binding to translocation ATPase

Helicase Motif III in SecA is essential for coupling preprotein binding to translocation ATPase

10.1038/sj.embor.7400206

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

The SecA ATPase is a protein translocase motor and a superfamily 2 (SF2) RNA helicase. The ATPase catalytic core (‘DEAD motor’) contains the seven conserved SF2 motifs. Here, we demonstrate that Motif III is essential for SecA‐mediated protein translocation and viability. SecA Motif III mutants can bind ligands (nucleotide, the SecYEG translocase ‘channel’, signal and mature preprotein domains), can catalyse basal and SecYEG‐stimulated ATP hydrolysis and can be activated for catalysis. However, Motif III mutation specifically blocks the preprotein‐stimulated ‘translocation ATPase’ at a step of the reaction pathway that lies downstream of ligand binding. A functional Motif III is required for optimal ligand‐driven conformational changes and kinetic parameters that underlie optimal preprotein‐modulated nucleotide cycling at the SecA DEAD motor. We propose that helicase Motif III couples preprotein binding to the SecA translocation ATPase and that catalytic activation of SF2 enzymes through Motif‐III‐mediated action is essential for both polypeptide and nucleic‐acid substrates.

Bibliography

Papanikou, E., Karamanou, S., Baud, C., Sianidis, G., Frank, M., & Economou, A. (2004). Helicase Motif III in SecA is essential for coupling preprotein binding to translocation ATPase. EMBO Reports, 5(8), 807â811. Portico.

Authors 6

Efrosyni Papanikou (first)
Spyridoula Karamanou (additional)
Catherine Baud (additional)
Giorgos Sianidis (additional)
Miriam Frank (additional)
Anastassios Economou (additional)

References 26 Referenced 24

10.1074/jbc.M200047200
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Dates

Type	When
Created	21 years, 1 month ago (July 23, 2004, 5:19 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:57 p.m.)
Indexed	1 year, 6 months ago (Feb. 23, 2024, 12:44 p.m.)
Issued	21 years, 1 month ago (Aug. 1, 2004)
Published	21 years, 1 month ago (Aug. 1, 2004)
Published Online	21 years, 1 month ago (Aug. 1, 2004)
Published Print	21 years, 1 month ago (Aug. 1, 2004)

Funders 0

None

BibTeX

@article{Papanikou_2004, title={Helicase Motif III in SecA is essential for coupling preprotein binding to translocation ATPase}, volume={5}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/sj.embor.7400206}, DOI={10.1038/sj.embor.7400206}, number={8}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Papanikou, Efrosyni and Karamanou, Spyridoula and Baud, Catherine and Sianidis, Giorgos and Frank, Miriam and Economou, Anastassios}, year={2004}, month=aug, pages={807–811} }

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