DOI: 10.1038/sj.embor.7400038. Involvement of the intermediate filament protein cytokeratin‐18 in actin pedestal formation during EPEC infection

Involvement of the intermediate filament protein cytokeratin‐18 in actin pedestal formation during EPEC infection

10.1038/sj.embor.7400038

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

While remaining extracellular, enteropathogenic Escherichia coli (EPEC) establish direct links with the cytoskeleton of the target epithelial cell leading to the formation of actin‐rich pedestals underneath attached bacteria. The translocated adaptor protein Tir forms the transmembrane bridge between the cytoskeleton and the bacterium; the extracellular domain of Tir functions as a receptor for the bacterial adhesin intimin, while the intracellular amino and carboxy termini interact with a number of focal adhesion and other cytoskeletal proteins; and recruitment of some is dependent on phosphorylation of Tyr 474. Using Tir as bait and HeLa cell cDNA library as prey in a yeast two‐hybrid screen, we identified cytokeratin 18 as a novel Tir partner protein. Cytokeratin 18 is recruited to the EPEC‐induced pedestal and has a direct role in actin accretion and cytoskeleton reorganization. This study is the first to implicate intermediate filaments in microfilament reorganization following EPEC infection.

Bibliography

Batchelor, M., Guignot, J., Patel, A., Cummings, N., Cleary, J., Knutton, S., Holden, D. W., Connerton, I., & Frankel, G. (2004). Involvement of the intermediate filament protein cytokeratinâ18 in actin pedestal formation during EPEC infection. EMBO Reports, 5(1), 104â110. Portico.

Authors 9

Miranda Batchelor (first)
Julie Guignot (additional)
Amit Patel (additional)
Nicola Cummings (additional)
Jennifer Cleary (additional)
Stuart Knutton (additional)
David W Holden (additional)
Ian Connerton (additional)
Gad Frankel (additional)

References 26 Referenced 75

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{'key': 'e_1_2_6_20_1', 'first-page': '119', 'article-title': 'Escherichia coli strains that cause diarrhoea but do not produce heat‐labile or heat‐stable enterotoxins and are non‐invasive', 'author': 'Levine MM', 'year': '1978', 'journal-title': 'Lancet'} / Lancet / Escherichia coli strains that cause diarrhoea but do not produce heat‐labile or heat‐stable enterotoxins and are non‐invasive by Levine MM (1978)
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Dates

Type	When
Created	21 years, 7 months ago (Jan. 7, 2004, 7:34 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:57 p.m.)
Indexed	4 months, 3 weeks ago (April 10, 2025, 3:29 p.m.)
Issued	21 years, 8 months ago (Jan. 1, 2004)
Published	21 years, 8 months ago (Jan. 1, 2004)
Published Online	21 years, 8 months ago (Jan. 1, 2004)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Batchelor_2004, title={Involvement of the intermediate filament protein cytokeratin‐18 in actin pedestal formation during EPEC infection}, volume={5}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/sj.embor.7400038}, DOI={10.1038/sj.embor.7400038}, number={1}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Batchelor, Miranda and Guignot, Julie and Patel, Amit and Cummings, Nicola and Cleary, Jennifer and Knutton, Stuart and Holden, David W and Connerton, Ian and Frankel, Gad}, year={2004}, month=jan, pages={104–110} }

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