DOI: 10.1038/sj.bjp.0703046. ADP can induce aggregation of human platelets via both P2Y1 and P2T receptors

ADP can induce aggregation of human platelets via both P2Y1 and P2T receptors

10.1038/sj.bjp.0703046

Crossref journal-article

Wiley

British Journal of Pharmacology (311)

Abstract

In the present study we have investigated the roles of P2Y1 and P2T receptor subtypes in adenosine 5′‐diphosphate (ADP)‐induced aggregation of human platelets in heparinized platelet rich plasma. The response to ADP can be characterized as the initial rate or the maximum or final extent of aggregation. The response profile is determined by the concentration of ADP used, being transient at lower and sustained at higher concentrations. The P2Y1 receptor antagonist, adenosine‐3′‐phosphate‐5′‐phosphate (A3P5P) competitively antagonized the initial rate of aggregation (pKB 5.47) and transformed the response profile to a slowly developing but sustained response. Both maximum and final extents were also inhibited by A3P5P although not in a competitive manner (Schild slope <1). The P2T receptor antagonist, AR‐C67085, competitively antagonized the final extent of aggregation (pKB 8.54), transforming the response profile to one of rapid, transient aggregation. Its effect on maximum extent (the most widely used index of aggregation) was complex, and further supported the involvement of both receptor subtypes in the aggregation response. ADP‐induced aggregation is a complex phenomenon, the nature of which is determined by the relative occupancy of the two receptor subtypes. While P2Y1 receptor activation causes a rapid and transient aggregation, the extent of sustained aggregation is determined by the level of P2T receptor occupancy. Hence, detailed analysis of the aggregation response is essential to correctly define the purinergic pharmacology of the platelet and interpretation of results is critically dependent on the response index chosen. British Journal of Pharmacology (2000) 129, 275–282; doi:10.1038/sj.bjp.0703046

Bibliography

Jarvis, G. E., Humphries, R. G., Robertson, M. J., & Leff, P. (2000). ADP can induce aggregation of human platelets via both P2Y1 and P2T receptors. British Journal of Pharmacology, 129(2), 275â282. Portico.

Authors 4

G E Jarvis (first)
R G Humphries (additional)
M J Robertson (additional)
P Leff (additional)

References 39 Referenced 64

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{'key': 'e_1_2_6_6_1', 'first-page': '1323', 'article-title': 'Identification of competitive antagonists of the P2Y1 receptor', 'volume': '50', 'author': 'BOYER J.L.', 'year': '1996', 'journal-title': 'Mol. Pharmacol.'} / Mol. Pharmacol. / Identification of competitive antagonists of the P2Y1 receptor by BOYER J.L. (1996)
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Dates

Type	When
Created	20 years, 7 months ago (Jan. 28, 2005, 3:40 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 5:06 a.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 10:53 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	16 years, 7 months ago (Jan. 30, 2009)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Jarvis_2000, title={ADP can induce aggregation of human platelets via both P2Y1 and P2T receptors}, volume={129}, ISSN={1476-5381}, url={http://dx.doi.org/10.1038/sj.bjp.0703046}, DOI={10.1038/sj.bjp.0703046}, number={2}, journal={British Journal of Pharmacology}, publisher={Wiley}, author={Jarvis, G E and Humphries, R G and Robertson, M J and Leff, P}, year={2000}, month=jan, pages={275–282} }

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