A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel
10.1038/s41564-018-0165-z
Crossref journal-article
Springer Science and Business Media LLC
Nature Microbiology (297)
Bibliography

Chetrit, D., Hu, B., Christie, P. J., Roy, C. R., & Liu, J. (2018). A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel. Nature Microbiology, 3(6), 678–686.

Authors 5
  1. David Chetrit (first)
  2. Bo Hu (additional)
  3. Peter J. Christie (additional)
  4. Craig R. Roy (additional)
  5. Jun Liu (additional)
References 38 Referenced 87
  1. Christie, P. J., Whitaker, N. & Gonzalez-Rivera, C. Mechanism and structure of the bacterial type IV secretion systems. Biochim. Biophys. Acta 1843, 1578–1591 (2014). (10.1016/j.bbamcr.2013.12.019) / Biochim. Biophys. Acta by PJ Christie (2014)
  2. Gonzalez-Rivera, C., Bhatty, M. & Christie, P. J. Mechanism and function of type IV secretion during infection of the human host. Microbiol. Spectr. 4, 1–29 (2016). (10.1128/microbiolspec.VMBF-0024-2015) / Microbiol. Spectr. by C Gonzalez-Rivera (2016)
  3. Costa, T. R. et al. Secretion systems in Gram-negative bacteria: structural and mechanistic insights. Nat. Rev. Microbiol. 13, 343–359 (2015). (10.1038/nrmicro3456) / Nat. Rev. Microbiol. by TR Costa (2015)
  4. Trokter, M., Felisberto-Rodrigues, C., Christie, P. J. & Waksman, G. Recent advances in the structural and molecular biology of type IV secretion systems. Curr. Opin. Struct. Biol. 27, 16–23 (2014). (10.1016/j.sbi.2014.02.006) / Curr. Opin. Struct. Biol. by M Trokter (2014)
  5. Kubori, T. & Nagai, H. The type IVB secretion system: an enigmatic chimera. Curr. Opin. Microbiol. 29, 22–29 (2015). (10.1016/j.mib.2015.10.001) / Curr. Opin. Microbiol. by T Kubori (2015)
  6. Low, H. H. et al. Structure of a type IV secretion system. Nature 508, 550–553 (2014). (10.1038/nature13081) / Nature by HH Low (2014)
  7. Kubori, T. et al. Native structure of a type IV secretion system core complex essential for Legionella pathogenesis. Proc. Natl Acad. Sci. USA 111, 11804–11809 (2014). (10.1073/pnas.1404506111) / Proc. Natl Acad. Sci. USA by T Kubori (2014)
  8. Ghosal, D., Chang, Y. W., Jeong, K. C., Vogel, J. P. & Jensen, G. J. In situ structure of the Legionella Dot/Icm type IV secretion system by electron cryotomography. EMBO Rep. 18, 726–732 (2017). (10.15252/embr.201643598) / EMBO Rep. by D Ghosal (2017)
  9. Jeong, K. C., Ghosal, D., Chang, Y. W., Jensen, G. J. & Vogel, J. P. Polar delivery of Legionella type IV secretion system substrates is essential for virulence. Proc. Natl Acad. Sci. USA 114, 8077–8082 (2017). (10.1073/pnas.1621438114) / Proc. Natl Acad. Sci. USA by KC Jeong (2017)
  10. Vincent, C. D. et al. Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol. Microbiol. 62, 1278–1291 (2006). (10.1111/j.1365-2958.2006.05446.x) / Mol. Microbiol. by CD Vincent (2006)
  11. Sexton, J. A., Yeo, H. J . & Vogel, J. P. Genetic analysis of the Legionella pneumophila DotB ATPase reveals a role in type IV secretion system protein export. Mol. Microbiol. 57, 70–84 (2005). (10.1111/j.1365-2958.2005.04667.x) / Mol. Microbiol. by JA Sexton (2005)
  12. Sutherland, M. C., Nguyen, T. L., Tseng, V. & Vogel, J. P. The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates. PLoS Path 8, e1002910 (2012). (10.1371/journal.ppat.1002910) / PLoS Path by MC Sutherland (2012)
  13. Vincent, C. D., Friedman, J. R., Jeong, K. C., Sutherland, M. C. & Vogel, J. P. Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system. Mol. Microbiol 85, 378–391 (2012). (10.1111/j.1365-2958.2012.08118.x) / Mol. Microbiol by CD Vincent (2012)
  14. Kwak, M. J. et al. Architecture of the type IV coupling protein complex of Legionella pneumophila . Nat. Microbiol. 2, 17114 (2017). (10.1038/nmicrobiol.2017.114) / Nat. Microbiol. by MJ Kwak (2017)
  15. Dang, T. A. & Christie, P. J. The VirB4 ATPase of Agrobacterium tumefaciens is a cytoplasmic membrane protein exposed at the periplasmic surface. J. Bacteriol. 179, 453–462 (1997). (10.1128/jb.179.2.453-462.1997) / J. Bacteriol. by TA Dang (1997)
  16. Dang, T. A., Zhou, X. R., Graf, B. & Christie, P. J. Dimerization of the Agrobacterium tumefaciens VirB4 ATPase and the effect of ATP-binding cassette mutations on the assembly and function of the T-DNA transporter. Mol. Microbiol 32, 1239–1253 (1999). (10.1046/j.1365-2958.1999.01436.x) / Mol. Microbiol by TA Dang (1999)
  17. Durand, E., Oomen, C. & Waksman, G. Biochemical dissection of the ATPase TraB, the VirB4 homologue of the Escherichia coli pKM101 conjugation machinery. J. Bacteriol. 192, 2315–2323 (2010). (10.1128/JB.01384-09) / J. Bacteriol. by E Durand (2010)
  18. Durand, E., Waksman, G. & Receveur-Brechot, V. Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system. BMC Struct. Biol. 11, 4 (2011). (10.1186/1472-6807-11-4) / BMC Struct. Biol. by E Durand (2011)
  19. Wallden, K. et al. Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system. Proc. Natl Acad. Sci. USA 109, 11348–11353 (2012). (10.1073/pnas.1201428109) / Proc. Natl Acad. Sci. USA by K Wallden (2012)
  20. Roy, C. R. & Isberg, R. R. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infect. Immun. 65, 571–578 (1997). (10.1128/iai.65.2.571-578.1997) / Infect. Immun. by CR Roy (1997)
  21. Buscher, B. A. et al. The DotL protein, a member of the TraG-coupling protein family, is essential for viability of Legionella pneumophila strain Lp02. J. Bacteriol. 187, 2927–2938 (2005). (10.1128/JB.187.9.2927-2938.2005) / J. Bacteriol. by BA Buscher (2005)
  22. Merriam, J. J., Mathur, R., Maxfield-Boumil, R. & Isberg, R. R. Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis. Infect. Immun. 65, 2497–2501 (1997). (10.1128/iai.65.6.2497-2501.1997) / Infect. Immun. by JJ Merriam (1997)
  23. Chetrit, D. et al. Negative regulation of the endocytic adaptor disabled-2 (Dab2) in mitosis. J. Biol. Chem. 286, 5392–5403 (2011). (10.1074/jbc.M110.161851) / J. Biol. Chem. by D Chetrit (2011)
  24. Zuckman, D. M., Hung, J. B. & Roy, C. R. Pore-forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth. Mol. Microbiol. 32, 990–1001 (1999). (10.1046/j.1365-2958.1999.01410.x) / Mol. Microbiol. by DM Zuckman (1999)
  25. Hu, B., Lara-Tejero, M., Kong, Q., Galan, J. E. & Liu, J. In situ molecular architecture of the Salmonella type III secretion machine. Cell 168, 1065–1074 (2017). (10.1016/j.cell.2017.02.022) / Cell by B Hu (2017)
  26. Morado, D. R., Hu, B. & Liu, J. Using Tomoauto: A protocol for high-throughput automated cryo-electron tomography. J. Vis. Exp. 107, e53608 (2016). / J. Vis. Exp. by DR Morado (2016)
  27. Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36–51 (2005). (10.1016/j.jsb.2005.07.007) / J. Struct. Biol. by DN Mastronarde (2005)
  28. Li, X. et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 10, 584–590 (2013). (10.1038/nmeth.2472) / Nat. Methods by X Li (2013)
  29. Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71–76 (1996). (10.1006/jsbi.1996.0013) / J. Struct. Biol. by JR Kremer (1996)
  30. Agulleiro, J. I. & Fernandez, J. J. Tomo3D 2.0—exploitation of advanced vector extensions (AVX) for 3D reconstruction. J. Struct. Biol. 189, 147–152 (2015). (10.1016/j.jsb.2014.11.009) / J. Struct. Biol. by JI Agulleiro (2015)
  31. Hu, B. et al. Visualization of the type III secretion sorting platform of Shigella flexneri. Proc. Natl Acad. Sci. USA 112, 1047–1052 (2015). (10.1073/pnas.1411610112) / Proc. Natl Acad. Sci. USA by B Hu (2015)
  32. Pettersen, E. F. et al. UCSF Chimera—a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004). (10.1002/jcc.20084) / J. Comput. Chem. by EF Pettersen (2004)
  33. Agard, D. A., Hiraoka, Y., Shaw, P. & Sedat, J. W. Fluorescence microscopy in three dimensions. Methods Cell Biol. 30, 353–377 (1989). (10.1016/S0091-679X(08)60986-3) / Methods Cell Biol. by DA Agard (1989)
  34. Frick-Cheng, A. E. et al. Molecular and structural analysis of the Helicobacter pylori cag type IV secretion system core complex. mBio 7, e02001–e02015 (2016). (10.1128/mBio.02001-15) / mBio by AE Frick-Cheng (2016)
  35. Berger, K. H. & Isberg, R. R. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol 7, 7–19 (1993). (10.1111/j.1365-2958.1993.tb01092.x) / Mol. Microbiol by KH Berger (1993)
  36. Andrews, H. L., Vogel, J. P. & Isberg, R. R. Identification of linked Legionella pneumophila genes essential for intracellular growth and evasion of the endocytic pathway. Infect. Immun. 66, 950–958 (1998). (10.1128/IAI.66.3.950-958.1998) / Infect. Immun. by HL Andrews (1998)
  37. Vogel, J. P., Andrews, H. L., Wong, S. K. & Isberg, R. R. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279, 873–876 (1998). (10.1126/science.279.5352.873) / Science by JP Vogel (1998)
  38. Finan, T. M., Kunkel, B., De Vos, G. F. & Signer, E. R. Second symbiotic megaplasmid in R hizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J. Bacteriol. 167, 66–72 (1986). (10.1128/jb.167.1.66-72.1986) / J. Bacteriol. by TM Finan (1986)
Dates
Type When
Created 7 years, 3 months ago (May 17, 2018, 5:39 a.m.)
Deposited 2 years, 8 months ago (Dec. 20, 2022, 6:50 p.m.)
Indexed 9 hours, 44 minutes ago (Aug. 21, 2025, 1:28 p.m.)
Issued 7 years, 3 months ago (May 21, 2018)
Published 7 years, 3 months ago (May 21, 2018)
Published Online 7 years, 3 months ago (May 21, 2018)
Funders 0

None

@article{Chetrit_2018, title={A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel}, volume={3}, ISSN={2058-5276}, url={http://dx.doi.org/10.1038/s41564-018-0165-z}, DOI={10.1038/s41564-018-0165-z}, number={6}, journal={Nature Microbiology}, publisher={Springer Science and Business Media LLC}, author={Chetrit, David and Hu, Bo and Christie, Peter J. and Roy, Craig R. and Liu, Jun}, year={2018}, month=may, pages={678–686} }