Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions
10.1038/s41467-018-04051-9
Crossref journal-article
Springer Science and Business Media LLC
Nature Communications (297)
Abstract

AbstractThe Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a “block-based” reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.

Bibliography

Zhu, D., Wang, X., Fang, Q., Van Etten, J. L., Rossmann, M. G., Rao, Z., & Zhang, X. (2018). Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. Nature Communications, 9(1).

Authors 7
  1. Dongjie Zhu (first)
  2. Xiangxi Wang (additional)
  3. Qianglin Fang (additional)
  4. James L Van Etten (additional)
  5. Michael G Rossmann (additional)
  6. Zihe Rao (additional)
  7. Xinzheng Zhang (additional)
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Dates
Type When
Created 7 years, 4 months ago (April 13, 2018, 6:08 a.m.)
Deposited 2 years, 8 months ago (Dec. 20, 2022, 8:46 a.m.)
Indexed 2 weeks ago (Aug. 6, 2025, 9:09 a.m.)
Issued 7 years, 4 months ago (April 19, 2018)
Published 7 years, 4 months ago (April 19, 2018)
Published Online 7 years, 4 months ago (April 19, 2018)
Funders 0

None

@article{Zhu_2018, title={Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions}, volume={9}, ISSN={2041-1723}, url={http://dx.doi.org/10.1038/s41467-018-04051-9}, DOI={10.1038/s41467-018-04051-9}, number={1}, journal={Nature Communications}, publisher={Springer Science and Business Media LLC}, author={Zhu, Dongjie and Wang, Xiangxi and Fang, Qianglin and Van Etten, James L and Rossmann, Michael G and Rao, Zihe and Zhang, Xinzheng}, year={2018}, month=apr }