DOI: 10.1038/nsmb1221. Spontaneous reverse movement of mRNA-bound tRNA through the ribosome

Spontaneous reverse movement of mRNA-bound tRNA through the ribosome

10.1038/nsmb1221

Crossref journal-article

Springer Science and Business Media LLC

Nature Structural & Molecular Biology (297)

Bibliography

Konevega, A. L., Fischer, N., Semenkov, Y. P., Stark, H., Wintermeyer, W., & Rodnina, M. V. (2007). Spontaneous reverse movement of mRNA-bound tRNA through the ribosome. Nature Structural & Molecular Biology, 14(4), 318â324.

Authors 6

Andrey L Konevega (first)
Niels Fischer (additional)
Yuri P Semenkov (additional)
Holger Stark (additional)
Wolfgang Wintermeyer (additional)
Marina V Rodnina (additional)

References 56 Referenced 82

Rodnina, M.V., Savelsbergh, A., Katunin, V.I. & Wintermeyer, W. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome. Nature 385, 37–41 (1997). (10.1038/385037a0) / Nature by MV Rodnina (1997)
Gavrilova, L.P. & Spirin, A.S. “Nonenzymatic” translation. Methods Enzymol. 30, 452–462 (1974). (10.1016/0076-6879(74)30045-6) / Methods Enzymol. by LP Gavrilova (1974)
Gavrilova, L.P., Kostiashkina, O.E., Koteliansky, V.E., Rutkevitch, N.M. & Spirin, A.S. Factor-free (“non-enzymic”) and factor-dependent systems of translation of polyuridylic acid by Escherichia coli ribosomes. J. Mol. Biol. 101, 537–552 (1976). (10.1016/0022-2836(76)90243-6) / J. Mol. Biol. by LP Gavrilova (1976)
Southworth, D.R., Brunelle, J.L. & Green, R. EF-G-independent translocation of the mRNA:tRNA complex is promoted by modification of the ribosome with thiol-specific reagents. J. Mol. Biol. 324, 611–623 (2002). (10.1016/S0022-2836(02)01196-8) / J. Mol. Biol. by DR Southworth (2002)
Fredrick, K. & Noller, H.F. Catalysis of ribosomal translocation by sparsomycin. Science 300, 1159–1162 (2003). (10.1126/science.1084571) / Science by K Fredrick (2003)
Semenkov, Y.P., Shapkina, T.G. & Kirillov, S.V. Puromycin reaction of the A-site bound peptidyl-tRNA. Biochimie 74, 411–417 (1992). (10.1016/0300-9084(92)90080-X) / Biochimie by YP Semenkov (1992)
Semenkov, Y., Shapkina, T., Makhno, V. & Kirillov, S. Puromycin reaction for the A site-bound peptidyl-tRNA. FEBS Lett. 296, 207–210 (1992). (10.1016/0014-5793(92)80380-Y) / FEBS Lett. by Y Semenkov (1992)
Moazed, D. & Noller, H.F. Intermediate states in the movement of transfer RNA in the ribosome. Nature 342, 142–148 (1989). (10.1038/342142a0) / Nature by D Moazed (1989)
Sharma, D., Southworth, D.R. & Green, R. EF-G-independent reactivity of a pre-translocation-state ribosome complex with the aminoacyl tRNA substrate puromycin supports an intermediate (hybrid) state of tRNA binding. RNA 10, 102–113 (2004). (10.1261/rna.5148704) / RNA by D Sharma (2004)
Hansen, J.L., Schmeing, T.M., Moore, P.B. & Steitz, T.A. Structural insights into peptide bond formation. Proc. Natl. Acad. Sci. USA 99, 11670–11675 (2002). (10.1073/pnas.172404099) / Proc. Natl. Acad. Sci. USA by JL Hansen (2002)
Cukras, A.R., Southworth, D.R., Brunelle, J.L., Culver, G.M. & Green, R. Ribosomal proteins S12 and S13 function as control elements for translocation of the mRNA:tRNA complex. Mol. Cell 12, 321–328 (2003). (10.1016/S1097-2765(03)00275-2) / Mol. Cell by AR Cukras (2003)
Savelsbergh, A. et al. An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation. Mol. Cell 11, 1517–1523 (2003). (10.1016/S1097-2765(03)00230-2) / Mol. Cell by A Savelsbergh (2003)
Frank, J. & Agrawal, R.K. A ratchet-like inter-subunit reorganization of the ribosome during translocation. Nature 406, 318–322 (2000). (10.1038/35018597) / Nature by J Frank (2000)
Spirin, A.S. Ribosomal translocation: facts and models. Prog. Nucleic Acid Res. Mol. Biol. 32, 75–114 (1985). (10.1016/S0079-6603(08)60346-3) / Prog. Nucleic Acid Res. Mol. Biol. by AS Spirin (1985)
Kirillov, S.V. & Semenkov Yu, P. Non-exclusion principle of Ac-Phe-tRNAPhe interaction with the donor and acceptor sites of Escherichia coli ribosomes. FEBS Lett. 148, 235–238 (1982). (10.1016/0014-5793(82)80814-4) / FEBS Lett. by SV Kirillov (1982)
Lill, R., Robertson, J.M. & Wintermeyer, W. Affinities of tRNA binding sites of ribosomes from Escherichia coli. Biochemistry 25, 3245–3255 (1986). (10.1021/bi00359a025) / Biochemistry by R Lill (1986)
Lill, R. & Wintermeyer, W. Destabilization of codon-anticodon interaction in the ribosomal exit site. J. Mol. Biol. 196, 137–148 (1987). (10.1016/0022-2836(87)90516-X) / J. Mol. Biol. by R Lill (1987)
Kirillov, S.V. & Semenkov Yu, P. Extension of Watson's model for the elongation cycle of protein biosynthesis. J. Biomol. Struct. Dyn. 4, 263–269 (1986). (10.1080/07391102.1986.10506345) / J. Biomol. Struct. Dyn. by SV Kirillov (1986)
Kirillov, S.V., Makarov, E.M. & Semenkov Yu, P. Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes. Role of 50 S subunits in formation of the E site. FEBS Lett. 157, 91–94 (1983). (10.1016/0014-5793(83)81122-3) / FEBS Lett. by SV Kirillov (1983)
Schilling-Bartetzko, S., Franceschi, F., Sternbach, H. & Nierhaus, K.H. Apparent association constants of tRNAs for the ribosomal A, P, and E sites. J. Biol. Chem. 267, 4693–4702 (1992). (10.1016/S0021-9258(18)42889-X) / J. Biol. Chem. by S Schilling-Bartetzko (1992)
Fahlman, R.P., Dale, T. & Uhlenbeck, O.C. Uniform binding of aminoacylated transfer RNAs to the ribosomal A and P sites. Mol. Cell 16, 799–805 (2004). (10.1016/j.molcel.2004.10.030) / Mol. Cell by RP Fahlman (2004)
Semenkov, Y.P., Rodnina, M.V. & Wintermeyer, W. The “allosteric three-site model” of elongation cannot be confirmed in a well-defined ribosome system from Escherichia coli. Proc. Natl. Acad. Sci. USA 93, 12183–12188 (1996). (10.1073/pnas.93.22.12183) / Proc. Natl. Acad. Sci. USA by YP Semenkov (1996)
Rheinberger, H.J. & Nierhaus, K.H. The ribosomal E site at low Mg2+: coordinate inactivation of ribosomal functions at Mg2+ concentrations below 10 mM and its prevention by polyamines. J. Biomol. Struct. Dyn. 5, 435–446 (1987). (10.1080/07391102.1987.10506403) / J. Biomol. Struct. Dyn. by HJ Rheinberger (1987)
Konevega, A.L. et al. Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions. RNA 10, 90–101 (2004). (10.1261/rna.5142404) / RNA by AL Konevega (2004)
Semenkov, Y.P., Rodnina, M.V. & Wintermeyer, W. Energetic contribution of tRNA hybrid state formation to translocation catalysis on the ribosome. Nat. Struct. Biol. 7, 1027–1031 (2000). (10.1038/80938) / Nat. Struct. Biol. by YP Semenkov (2000)
Gold, L. Post-transcriptional regulatory mechanisms in E. coli. Annu. Rev. Biochem. 57, 199–233 (1988). (10.1146/annurev.bi.57.070188.001215) / Annu. Rev. Biochem. by L Gold (1988)
Jerinic, O. & Joseph, S. Conformational changes in the ribosome induced by translational miscoding agents. J. Mol. Biol. 304, 707–713 (2000). (10.1006/jmbi.2000.4269) / J. Mol. Biol. by O Jerinic (2000)
Yusupov, M.M. et al. Crystal structure of the ribosome at 5.5 Å resolution. Science 292, 883–896 (2001). (10.1126/science.1060089) / Science by MM Yusupov (2001)
Selmer, M. et al. Structure of the 70S ribosome complexed with mRNA and tRNA. Science 313, 1935–1942 (2006). (10.1126/science.1131127) / Science by M Selmer (2006)
Rheinberger, H.J. & Nierhaus, K.H. Adjacent codon-anticodon interactions of both tRNAs present at the ribosomal A and P or P and E sites. FEBS Lett. 204, 97–99 (1986). (10.1016/0014-5793(86)81393-X) / FEBS Lett. by HJ Rheinberger (1986)
Lill, R. et al. Specific recognition of the 3′-terminal adenosine of tRNAPhe in the exit site of Escherichia coli ribosomes. J. Mol. Biol. 203, 699–705 (1988). (10.1016/0022-2836(88)90203-3) / J. Mol. Biol. by R Lill (1988)
Gabashvili, I.S. et al. Solution structure of the E. coli 70S ribosome at 11.5 Å resolution. Cell 100, 537–549 (2000). (10.1016/S0092-8674(00)80690-X) / Cell by IS Gabashvili (2000)
Berk, V., Zhang, W., Pai, R.D. & Doudna Cate, J.H. Structural basis for mRNA and tRNA positioning on the ribosome. Proc. Natl. Acad. Sci. USA 103, 15830–15834 (2006). (10.1073/pnas.0607541103) / Proc. Natl. Acad. Sci. USA by V Berk (2006)
Harms, J. et al. High resolution structure of the large ribosomal subunit from a mesophilic eubacterium. Cell 107, 679–688 (2001). (10.1016/S0092-8674(01)00546-3) / Cell by J Harms (2001)
Valle, M. et al. Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process. EMBO J. 21, 3557–3567 (2002). (10.1093/emboj/cdf326) / EMBO J. by M Valle (2002)
Gao, H., Valle, M., Ehrenberg, M. & Frank, J. Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset. J. Struct. Biol. 147, 283–290 (2004). (10.1016/j.jsb.2004.02.008) / J. Struct. Biol. by H Gao (2004)
Valle, M. et al. Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy. Nat. Struct. Biol. 10, 899–906 (2003). (10.1038/nsb1003) / Nat. Struct. Biol. by M Valle (2003)
Shoji, S., Walker, S.E. & Fredrick, K. Reverse translocation of tRNA in the ribosome. Mol. Cell 24, 931–942 (2006). (10.1016/j.molcel.2006.11.025) / Mol. Cell by S Shoji (2006)
Agrawal, R.K. et al. Effect of buffer conditions on the position of tRNA on the 70 S ribosome as visualized by cryoelectron microscopy. J. Biol. Chem. 274, 8723–8729 (1999). (10.1074/jbc.274.13.8723) / J. Biol. Chem. by RK Agrawal (1999)
Robertson, J.M. & Wintermeyer, W. Mechanism of ribosomal translocation. tRNA binds transiently to an exit site before leaving the ribosome during translocation. J. Mol. Biol. 196, 525–540 (1987). (10.1016/0022-2836(87)90030-1) / J. Mol. Biol. by JM Robertson (1987)
Qin, Y. et al. The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. Cell 127, 721–733 (2006). (10.1016/j.cell.2006.09.037) / Cell by Y Qin (2006)
Ramakrishnan, V. Ribosome structure and the mechanism of translation. Cell 108, 557–572 (2002). (10.1016/S0092-8674(02)00619-0) / Cell by V Ramakrishnan (2002)
Frank, J. & Agrawal, R.K. A ratchet-like inter-subunit reorganization of the ribosme during translocation. Nature 406, 318–322 (2000). (10.1038/35018597) / Nature by J Frank (2000)
Peske, F., Savelsbergh, A., Katunin, V.I., Rodnina, M.V. & Wintermeyer, W. Conformational changes of the small ribosomal subunit during elongation factor G-dependent tRNA-mRNA translocation. J. Mol. Biol. 343, 1183–1194 (2004). (10.1016/j.jmb.2004.08.097) / J. Mol. Biol. by F Peske (2004)
Savelsbergh, A., Matassova, N.B., Rodnina, M.V. & Wintermeyer, W. Role of domains 4 and 5 in elongation factor G functions on the ribosome. J. Mol. Biol. 300, 951–961 (2000). (10.1006/jmbi.2000.3886) / J. Mol. Biol. by A Savelsbergh (2000)
Mohr, D., Wintermeyer, W. & Rodnina, M.V. Arginines 29 and 59 of elongation factor G are important for GTP hydrolysis or translocation on the ribosome. EMBO J. 19, 3458–3464 (2000). (10.1093/emboj/19.13.3458) / EMBO J. by D Mohr (2000)
Wilson, K.S. & Noller, H.F. Mapping the position of translational elongation factor EF- G in the ribosome by directed hydroxyl radical probing. Cell 92, 131–139 (1998). (10.1016/S0092-8674(00)80905-8) / Cell by KS Wilson (1998)
Agrawal, R.K., Penczek, P., Grassucci, R.A. & Frank, J. Visualization of elongation factor G on the Escherichia coli 70S ribosome: The mechanism of translocation. Proc. Natl. Acad. Sci. USA 95, 6134–6138 (1998). (10.1073/pnas.95.11.6134) / Proc. Natl. Acad. Sci. USA by RK Agrawal (1998)
Stark, H., Rodnina, M.V., Wieden, H.-J., van Heel, M. & Wintermeyer, W. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation. Cell 100, 301–309 (2000). (10.1016/S0092-8674(00)80666-2) / Cell by H Stark (2000)
Pestova, T.V., Hellen, C.U. & Shatsky, I.N. Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry. Mol. Cell. Biol. 16, 6859–6869 (1996). (10.1128/MCB.16.12.6859) / Mol. Cell. Biol. by TV Pestova (1996)
Dubochet, J. et al. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21, 129–228 (1988). (10.1017/S0033583500004297) / Q. Rev. Biophys. by J Dubochet (1988)
Sander, B., Golas, M.M. & Stark, H. Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy. J. Struct. Biol. 151, 92–105 (2005). (10.1016/j.jsb.2005.04.004) / J. Struct. Biol. by B Sander (2005)
Ludtke, S.J., Baldwin, P.R. & Chiu, W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128, 82–97 (1999). (10.1006/jsbi.1999.4174) / J. Struct. Biol. by SJ Ludtke (1999)
Sander, B., Golas, M.M. & Stark, H. Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra. J. Struct. Biol. 142, 392–401 (2003). (10.1016/S1047-8477(03)00072-8) / J. Struct. Biol. by B Sander (2003)
van Heel, M., Harauz, G., Orlova, E.V., Schmidt, R. & Schatz, M. A new generation of the IMAGIC image processing system. J. Struct. Biol. 116, 17–24 (1996). (10.1006/jsbi.1996.0004) / J. Struct. Biol. by M van Heel (1996)
Sander, B., Golas, M.M. & Stark, H. Corrim-based alignment for improved speed in single-particle image processing. J. Struct. Biol. 143, 219–228 (2003). (10.1016/j.jsb.2003.08.001) / J. Struct. Biol. by B Sander (2003)

Dates

Type	When
Created	18 years, 5 months ago (March 18, 2007, 2:13 p.m.)
Deposited	2 years, 3 months ago (May 19, 2023, 12:50 a.m.)
Indexed	2 months, 3 weeks ago (June 12, 2025, 4:06 a.m.)
Issued	18 years, 5 months ago (March 18, 2007)
Published	18 years, 5 months ago (March 18, 2007)
Published Online	18 years, 5 months ago (March 18, 2007)
Published Print	18 years, 5 months ago (April 1, 2007)

Funders 0

None

BibTeX

@article{Konevega_2007, title={Spontaneous reverse movement of mRNA-bound tRNA through the ribosome}, volume={14}, ISSN={1545-9985}, url={http://dx.doi.org/10.1038/nsmb1221}, DOI={10.1038/nsmb1221}, number={4}, journal={Nature Structural & Molecular Biology}, publisher={Springer Science and Business Media LLC}, author={Konevega, Andrey L and Fischer, Niels and Semenkov, Yuri P and Stark, Holger and Wintermeyer, Wolfgang and Rodnina, Marina V}, year={2007}, month=mar, pages={318–324} }

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  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2007,
        3,
        18
      ]
    ]
  },
  "references-count": 56,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2007,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "BFnsmb1221"
  ],
  "URL": "http://dx.doi.org/10.1038/nsmb1221",
  "relation": {},
  "ISSN": [
    "1545-9993",
    "1545-9985"
  ],
  "subject": [],
  "container-title-short": "Nat Struct Mol Biol",
  "published": {
    "date-parts": [
      [
        2007,
        3,
        18
      ]
    ]
  }
}