DOI: 10.1038/nsmb.2011. mRNA translocation occurs during the second step of ribosomal intersubunit rotation

mRNA translocation occurs during the second step of ribosomal intersubunit rotation

10.1038/nsmb.2011

Crossref journal-article

Springer Science and Business Media LLC

Nature Structural & Molecular Biology (297)

Bibliography

Ermolenko, D. N., & Noller, H. F. (2011). mRNA translocation occurs during the second step of ribosomal intersubunit rotation. Nature Structural & Molecular Biology, 18(4), 457â462.

Authors 2

Dmitri N Ermolenko (first)
Harry F Noller (additional)

References 51 Referenced 125

Cate, J.H., Yusupov, M.M., Yusupova, G.Z., Earnest, T.N. & Noller, H.F. X-ray crystal structures of 70S ribosome functional complexes. Science 285, 2095–2104 (1999). (10.1126/science.285.5436.2095) / Science by JH Cate (1999)
Korostelev, A., Trakhanov, S., Laurberg, M. & Noller, H.F. Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements. Cell 126, 1065–1077 (2006). (10.1016/j.cell.2006.08.032) / Cell by A Korostelev (2006)
Selmer, M. et al. Structure of the 70S ribosome complexed with mRNA and tRNA. Science 313, 1935–1942 (2006). (10.1126/science.1131127) / Science by M Selmer (2006)
Yusupov, M.M. et al. Crystal structure of the ribosome at 5.5 Å resolution. Science 292, 883–896 (2001). (10.1126/science.1060089) / Science by MM Yusupov (2001)
Yusupova, G.Z., Yusupov, M.M., Cate, J.H. & Noller, H.F. The path of messenger RNA through the ribosome. Cell 106, 233–241 (2001). (10.1016/S0092-8674(01)00435-4) / Cell by GZ Yusupova (2001)
Moazed, D. & Noller, H.F. Intermediate states in the movement of transfer RNA in the ribosome. Nature 342, 142–148 (1989). (10.1038/342142a0) / Nature by D Moazed (1989)
Spiegel, P.C., Ermolenko, D.N. & Noller, H.F. Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome. RNA 13, 1473–1482 (2007). (10.1261/rna.601507) / RNA by PC Spiegel (2007)
Pan, D., Kirillov, S.V. & Cooperman, B.S. Kinetically competent intermediates in the translocation step of protein synthesis. Mol. Cell 25, 519–529 (2007). (10.1016/j.molcel.2007.01.014) / Mol. Cell by D Pan (2007)
Frank, J. & Agrawal, R.K. A ratchet-like inter-subunit reorganization of the ribosome during translocation. Nature 406, 318–322 (2000). (10.1038/35018597) / Nature by J Frank (2000)
Gao, H. et al. Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement. Cell 113, 789–801 (2003). (10.1016/S0092-8674(03)00427-6) / Cell by H Gao (2003)
Valle, M. et al. Locking and unlocking of ribosomal motions. Cell 114, 123–134 (2003). (10.1016/S0092-8674(03)00476-8) / Cell by M Valle (2003)
Frank, J., Gao, H., Sengupta, J., Gao, N. & Taylor, D.J. The process of mRNA-tRNA translocation. Proc. Natl. Acad. Sci. USA 104, 19671–19678 (2007). (10.1073/pnas.0708517104) / Proc. Natl. Acad. Sci. USA by J Frank (2007)
Horan, L.H. & Noller, H.F. Intersubunit movement is required for ribosomal translocation. Proc. Natl. Acad. Sci. USA 104, 4881–4885 (2007). (10.1073/pnas.0700762104) / Proc. Natl. Acad. Sci. USA by LH Horan (2007)
Ermolenko, D.N. et al. The antibiotic viomycin traps the ribosome in an intermediate state of translocation. Nat. Struct. Mol. Biol. 14, 493–497 (2007). (10.1038/nsmb1243) / Nat. Struct. Mol. Biol. by DN Ermolenko (2007)
Agirrezabala, X. et al. Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome. Mol. Cell 32, 190–197 (2008). (10.1016/j.molcel.2008.10.001) / Mol. Cell by X Agirrezabala (2008)
Julián, P. et al. Structure of ratcheted ribosomes with tRNAs in hybrid states. Proc. Natl. Acad. Sci. USA 105, 16924–16927 (2008). (10.1073/pnas.0809587105) / Proc. Natl. Acad. Sci. USA by P Julián (2008)
Cornish, P.V., Ermolenko, D.N., Noller, H.F. & Ha, T. Spontaneous intersubunit rotation in single ribosomes. Mol. Cell 30, 578–588 (2008). (10.1016/j.molcel.2008.05.004) / Mol. Cell by PV Cornish (2008)
Blanchard, S.C., Kim, H.D., Gonzalez, R.L. Jr., Puglisi, J.D. & Chu, S. tRNA dynamics on the ribosome during translation. Proc. Natl. Acad. Sci. USA 101, 12893–12898 (2004). (10.1073/pnas.0403884101) / Proc. Natl. Acad. Sci. USA by SC Blanchard (2004)
Ermolenko, D.N. et al. Observation of intersubunit movement of the ribosome in solution using FRET. J. Mol. Biol. 370, 530–540 (2007). (10.1016/j.jmb.2007.04.042) / J. Mol. Biol. by DN Ermolenko (2007)
Fei, J. et al. Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation. Proc. Natl. Acad. Sci. USA 106, 15702–15707 (2009). (10.1073/pnas.0908077106) / Proc. Natl. Acad. Sci. USA by J Fei (2009)
Fei, J., Kosuri, P., MacDougall, D.D. & Gonzalez, R.L. Jr. Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation. Mol. Cell 30, 348–359 (2008). (10.1016/j.molcel.2008.03.012) / Mol. Cell by J Fei (2008)
Munro, J.B., Altman, R.B., O′Connor, N. & Blanchard, S.C. Identification of two distinct hybrid state intermediates on the ribosome. Mol. Cell 25, 505–517 (2007). (10.1016/j.molcel.2007.01.022) / Mol. Cell by JB Munro (2007)
Lill, R. et al. Specific recognition of the 3′-terminal adenosine of tRNAPhe in the exit site of Escherichia coli ribosomes. J. Mol. Biol. 203, 699–705 (1988). (10.1016/0022-2836(88)90203-3) / J. Mol. Biol. by R Lill (1988)
Studer, S.M., Feinberg, J.S. & Joseph, S. Rapid kinetic analysis of EF-G-dependent mRNA translocation in the ribosome. J. Mol. Biol. 327, 369–381 (2003). (10.1016/S0022-2836(03)00146-3) / J. Mol. Biol. by SM Studer (2003)
Munro, J.B. et al. Spontaneous formation of the unlocked state of the ribosome is a multistep process. Proc. Natl. Acad. Sci. USA 107, 709–714 (2010). (10.1073/pnas.0908597107) / Proc. Natl. Acad. Sci. USA by JB Munro (2010)
Peske, F., Savelsbergh, A., Katunin, V.I., Rodnina, M.V. & Wintermeyer, W. Conformational changes of the small ribosomal subunit during elongation factor G-dependent tRNA-mRNA translocation. J. Mol. Biol. 343, 1183–1194 (2004). (10.1016/j.jmb.2004.08.097) / J. Mol. Biol. by F Peske (2004)
Shi, X., Chiu, K., Ghosh, S. & Joseph, S. Bases in 16S rRNA important for subunit association, tRNA binding, and translocation. Biochemistry 48, 6772–6782 (2009). (10.1021/bi900472a) / Biochemistry by X Shi (2009)
Dorner, S., Brunelle, J.L., Sharma, D. & Green, R. The hybrid state of tRNA binding is an authentic translation elongation intermediate. Nat. Struct. Mol. Biol. 13, 234–241 (2006). (10.1038/nsmb1060) / Nat. Struct. Mol. Biol. by S Dorner (2006)
Borovinskaya, M.A., Shoji, S., Fredrick, K. & Cate, J.H. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA 14, 1590–1599 (2008). (10.1261/rna.1076908) / RNA by MA Borovinskaya (2008)
Borovinskaya, M.A., Shoji, S., Holton, J.M., Fredrick, K. & Cate, J.H. A steric block in translation caused by the antibiotic spectinomycin. ACS Chem. Biol. 2, 545–552 (2007). (10.1021/cb700100n) / ACS Chem. Biol. by MA Borovinskaya (2007)
Zavialov, A.V., Hauryliuk, V.V. & Ehrenberg, M. Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs. J. Biol. 4, 9 (2005). (10.1186/jbiol24) / J. Biol. by AV Zavialov (2005)
Belitsina, N.V., Glukhova, M.A. & Spirin, A.S. Elongation factor G-promoted translocation and polypeptide elongation in ribosomes without GTP cleavage: use of columns with matrix-bound polyuridylic acid. Methods Enzymol. 60, 761–779 (1979). (10.1016/S0076-6879(79)60070-8) / Methods Enzymol. by NV Belitsina (1979)
Bodley, J.W., Zieve, F.J., Lin, L. & Zieve, S.T. Formation of the ribosome-G factor-GDP complex in the presence of fusidic acid. Biochem. Biophys. Res. Commun. 37, 437–443 (1969). (10.1016/0006-291X(69)90934-6) / Biochem. Biophys. Res. Commun. by JW Bodley (1969)
Inoue-Yokosawa, N., Ishikawa, C. & Kaziro, Y. The role of guanosine triphosphate in translocation reaction catalyzed by elongation factor G. J. Biol. Chem. 249, 4321–4323 (1974). (10.1016/S0021-9258(19)42519-2) / J. Biol. Chem. by N Inoue-Yokosawa (1974)
Ticu, C., Nechifor, R., Nguyen, B., Desrosiers, M. & Wilson, K.S. Conformational changes in switch I of EF-G drive its directional cycling on and off the ribosome. EMBO J. 28, 2053–2065 (2009). (10.1038/emboj.2009.169) / EMBO J. by C Ticu (2009)
Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Distinct functions of elongation factor G in ribosome recycling and translocation. RNA 15, 772–780 (2009). (10.1261/rna.1592509) / RNA by A Savelsbergh (2009)
Rodnina, M.V., Savelsbergh, A., Katunin, V.I. & Wintermeyer, W. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome. Nature 385, 37–41 (1997). (10.1038/385037a0) / Nature by MV Rodnina (1997)
Savelsbergh, A. et al. An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation. Mol. Cell 11, 1517–1523 (2003). (10.1016/S1097-2765(03)00230-2) / Mol. Cell by A Savelsbergh (2003)
Schuwirth, B.S. et al. Structures of the bacterial ribosome at 3.5 A resolution. Science 310, 827–834 (2005). (10.1126/science.1117230) / Science by BS Schuwirth (2005)
Ali, I.K., Lancaster, L., Feinberg, J., Joseph, S. & Noller, H.F. Deletion of a conserved, central ribosomal intersubunit RNA bridge. Mol. Cell 23, 865–874 (2006). (10.1016/j.molcel.2006.08.011) / Mol. Cell by IK Ali (2006)
Feinberg, J.S. & Joseph, S. Ribose 2′-hydroxyl groups in the 5′ strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation. RNA 12, 580–588 (2006). (10.1261/rna.2290706) / RNA by JS Feinberg (2006)
Feldman, M.B., Terry, D.S., Altman, R.B. & Blanchard, S.C. Aminoglycoside activity observed on single pre-translocation ribosome complexes. Nat. Chem. Biol. 6, 54–62 (2010). (10.1038/nchembio.274) / Nat. Chem. Biol. by MB Feldman (2010)
Katunin, V.I., Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Coupling of GTP hydrolysis by elongation factor G to translocation and factor recycling on the ribosome. Biochemistry 41, 12806–12812 (2002). (10.1021/bi0264871) / Biochemistry by VI Katunin (2002)
Robertson, J.M., Paulsen, H. & Wintermeyer, W. Pre-steady-state kinetics of ribosomal translocation. J. Mol. Biol. 192, 351–360 (1986). (10.1016/0022-2836(86)90370-0) / J. Mol. Biol. by JM Robertson (1986)
Gao, Y.G. et al. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 326, 694–699 (2009). (10.1126/science.1179709) / Science by YG Gao (2009)
Martemyanov, K.A., Yarunin, A.S., Liljas, A. & Gudkov, A.T. An intact conformation at the tip of elongation factor G domain IV is functionally important. FEBS Lett. 434, 205–208 (1998). (10.1016/S0014-5793(98)00982-X) / FEBS Lett. by KA Martemyanov (1998)
Peske, F., Matassova, N.B., Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Conformationally restricted elongation factor G retains GTPase activity but is inactive in translocation on the ribosome. Mol. Cell 6, 501–505 (2000). (10.1016/S1097-2765(00)00049-6) / Mol. Cell by F Peske (2000)
Wilson, K.S. & Noller, H.F. Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing. Cell 92, 131–139 (1998). (10.1016/S0092-8674(00)80905-8) / Cell by KS Wilson (1998)
Culver, G.M. & Noller, H.F. Efficient reconstitution of functional Escherichia coli 30S ribosomal subunits from a complete set of recombinant small subunit ribosomal proteins. RNA 5, 832–843 (1999). (10.1017/S1355838299990714) / RNA by GM Culver (1999)
Hickerson, R., Majumdar, Z.K., Baucom, A., Clegg, R.M. & Noller, H.F. Measurement of internal movements within the 30 S ribosomal subunit using Forster resonance energy transfer. J. Mol. Biol. 354, 459–472 (2005). (10.1016/j.jmb.2005.09.010) / J. Mol. Biol. by R Hickerson (2005)
Lieberman, K.R. et al. The 23 S rRNA environment of ribosomal protein L9 in the 50 S ribosomal subunit. J. Mol. Biol. 297, 1129–1143 (2000). (10.1006/jmbi.2000.3621) / J. Mol. Biol. by KR Lieberman (2000)

Dates

Type	When
Created	14 years, 5 months ago (March 13, 2011, 3:25 p.m.)
Deposited	2 years, 3 months ago (May 19, 2023, 12:29 a.m.)
Indexed	2 months, 1 week ago (June 12, 2025, 4:06 a.m.)
Issued	14 years, 5 months ago (March 13, 2011)
Published	14 years, 5 months ago (March 13, 2011)
Published Online	14 years, 5 months ago (March 13, 2011)
Published Print	14 years, 4 months ago (April 1, 2011)

Funders 0

None

BibTeX

@article{Ermolenko_2011, title={mRNA translocation occurs during the second step of ribosomal intersubunit rotation}, volume={18}, ISSN={1545-9985}, url={http://dx.doi.org/10.1038/nsmb.2011}, DOI={10.1038/nsmb.2011}, number={4}, journal={Nature Structural & Molecular Biology}, publisher={Springer Science and Business Media LLC}, author={Ermolenko, Dmitri N and Noller, Harry F}, year={2011}, month=mar, pages={457–462} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        12
      ]
    ],
    "date-time": "2025-06-12T08:06:57Z",
    "timestamp": 1749715617402
  },
  "reference-count": 51,
  "publisher": "Springer Science and Business Media LLC",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2011,
            3,
            13
          ]
        ],
        "date-time": "2011-03-13T00:00:00Z",
        "timestamp": 1299974400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "http://www.springer.com/tdm"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2011,
        4
      ]
    ]
  },
  "DOI": "10.1038/nsmb.2011",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        3,
        13
      ]
    ],
    "date-time": "2011-03-13T19:25:22Z",
    "timestamp": 1300044322000
  },
  "page": "457-462",
  "source": "Crossref",
  "is-referenced-by-count": 125,
  "title": "mRNA translocation occurs during the second step of ribosomal intersubunit rotation",
  "prefix": "10.1038",
  "volume": "18",
  "author": [
    {
      "given": "Dmitri N",
      "family": "Ermolenko",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Harry F",
      "family": "Noller",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "297",
  "published-online": {
    "date-parts": [
      [
        2011,
        3,
        13
      ]
    ]
  },
  "reference": [
    {
      "key": "BFnsmb2011_CR1",
      "doi-asserted-by": "publisher",
      "first-page": "2095",
      "DOI": "10.1126/science.285.5436.2095",
      "volume": "285",
      "author": "JH Cate",
      "year": "1999",
      "unstructured": "Cate, J.H., Yusupov, M.M., Yusupova, G.Z., Earnest, T.N. & Noller, H.F. X-ray crystal structures of 70S ribosome functional complexes. Science 285, 2095\u20132104 (1999).",
      "journal-title": "Science"
    },
    {
      "key": "BFnsmb2011_CR2",
      "doi-asserted-by": "publisher",
      "first-page": "1065",
      "DOI": "10.1016/j.cell.2006.08.032",
      "volume": "126",
      "author": "A Korostelev",
      "year": "2006",
      "unstructured": "Korostelev, A., Trakhanov, S., Laurberg, M. & Noller, H.F. Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements. Cell 126, 1065\u20131077 (2006).",
      "journal-title": "Cell"
    },
    {
      "key": "BFnsmb2011_CR3",
      "doi-asserted-by": "publisher",
      "first-page": "1935",
      "DOI": "10.1126/science.1131127",
      "volume": "313",
      "author": "M Selmer",
      "year": "2006",
      "unstructured": "Selmer, M. et al. Structure of the 70S ribosome complexed with mRNA and tRNA. Science 313, 1935\u20131942 (2006).",
      "journal-title": "Science"
    },
    {
      "key": "BFnsmb2011_CR4",
      "doi-asserted-by": "publisher",
      "first-page": "883",
      "DOI": "10.1126/science.1060089",
      "volume": "292",
      "author": "MM Yusupov",
      "year": "2001",
      "unstructured": "Yusupov, M.M. et al. Crystal structure of the ribosome at 5.5 \u00c5 resolution. Science 292, 883\u2013896 (2001).",
      "journal-title": "Science"
    },
    {
      "key": "BFnsmb2011_CR5",
      "doi-asserted-by": "publisher",
      "first-page": "233",
      "DOI": "10.1016/S0092-8674(01)00435-4",
      "volume": "106",
      "author": "GZ Yusupova",
      "year": "2001",
      "unstructured": "Yusupova, G.Z., Yusupov, M.M., Cate, J.H. & Noller, H.F. The path of messenger RNA through the ribosome. Cell 106, 233\u2013241 (2001).",
      "journal-title": "Cell"
    },
    {
      "key": "BFnsmb2011_CR6",
      "doi-asserted-by": "publisher",
      "first-page": "142",
      "DOI": "10.1038/342142a0",
      "volume": "342",
      "author": "D Moazed",
      "year": "1989",
      "unstructured": "Moazed, D. & Noller, H.F. Intermediate states in the movement of transfer RNA in the ribosome. Nature 342, 142\u2013148 (1989).",
      "journal-title": "Nature"
    },
    {
      "key": "BFnsmb2011_CR7",
      "doi-asserted-by": "publisher",
      "first-page": "1473",
      "DOI": "10.1261/rna.601507",
      "volume": "13",
      "author": "PC Spiegel",
      "year": "2007",
      "unstructured": "Spiegel, P.C., Ermolenko, D.N. & Noller, H.F. Elongation factor G stabilizes the hybrid-state conformation of the 70S ribosome. RNA 13, 1473\u20131482 (2007).",
      "journal-title": "RNA"
    },
    {
      "key": "BFnsmb2011_CR8",
      "doi-asserted-by": "publisher",
      "first-page": "519",
      "DOI": "10.1016/j.molcel.2007.01.014",
      "volume": "25",
      "author": "D Pan",
      "year": "2007",
      "unstructured": "Pan, D., Kirillov, S.V. & Cooperman, B.S. Kinetically competent intermediates in the translocation step of protein synthesis. Mol. Cell 25, 519\u2013529 (2007).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR9",
      "doi-asserted-by": "publisher",
      "first-page": "318",
      "DOI": "10.1038/35018597",
      "volume": "406",
      "author": "J Frank",
      "year": "2000",
      "unstructured": "Frank, J. & Agrawal, R.K. A ratchet-like inter-subunit reorganization of the ribosome during translocation. Nature 406, 318\u2013322 (2000).",
      "journal-title": "Nature"
    },
    {
      "key": "BFnsmb2011_CR10",
      "doi-asserted-by": "publisher",
      "first-page": "789",
      "DOI": "10.1016/S0092-8674(03)00427-6",
      "volume": "113",
      "author": "H Gao",
      "year": "2003",
      "unstructured": "Gao, H. et al. Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement. Cell 113, 789\u2013801 (2003).",
      "journal-title": "Cell"
    },
    {
      "key": "BFnsmb2011_CR11",
      "doi-asserted-by": "publisher",
      "first-page": "123",
      "DOI": "10.1016/S0092-8674(03)00476-8",
      "volume": "114",
      "author": "M Valle",
      "year": "2003",
      "unstructured": "Valle, M. et al. Locking and unlocking of ribosomal motions. Cell 114, 123\u2013134 (2003).",
      "journal-title": "Cell"
    },
    {
      "key": "BFnsmb2011_CR12",
      "doi-asserted-by": "publisher",
      "first-page": "19671",
      "DOI": "10.1073/pnas.0708517104",
      "volume": "104",
      "author": "J Frank",
      "year": "2007",
      "unstructured": "Frank, J., Gao, H., Sengupta, J., Gao, N. & Taylor, D.J. The process of mRNA-tRNA translocation. Proc. Natl. Acad. Sci. USA 104, 19671\u201319678 (2007).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR13",
      "doi-asserted-by": "publisher",
      "first-page": "4881",
      "DOI": "10.1073/pnas.0700762104",
      "volume": "104",
      "author": "LH Horan",
      "year": "2007",
      "unstructured": "Horan, L.H. & Noller, H.F. Intersubunit movement is required for ribosomal translocation. Proc. Natl. Acad. Sci. USA 104, 4881\u20134885 (2007).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR14",
      "doi-asserted-by": "publisher",
      "first-page": "493",
      "DOI": "10.1038/nsmb1243",
      "volume": "14",
      "author": "DN Ermolenko",
      "year": "2007",
      "unstructured": "Ermolenko, D.N. et al. The antibiotic viomycin traps the ribosome in an intermediate state of translocation. Nat. Struct. Mol. Biol. 14, 493\u2013497 (2007).",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR15",
      "doi-asserted-by": "publisher",
      "first-page": "190",
      "DOI": "10.1016/j.molcel.2008.10.001",
      "volume": "32",
      "author": "X Agirrezabala",
      "year": "2008",
      "unstructured": "Agirrezabala, X. et al. Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome. Mol. Cell 32, 190\u2013197 (2008).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR16",
      "doi-asserted-by": "publisher",
      "first-page": "16924",
      "DOI": "10.1073/pnas.0809587105",
      "volume": "105",
      "author": "P Juli\u00e1n",
      "year": "2008",
      "unstructured": "Juli\u00e1n, P. et al. Structure of ratcheted ribosomes with tRNAs in hybrid states. Proc. Natl. Acad. Sci. USA 105, 16924\u201316927 (2008).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR17",
      "doi-asserted-by": "publisher",
      "first-page": "578",
      "DOI": "10.1016/j.molcel.2008.05.004",
      "volume": "30",
      "author": "PV Cornish",
      "year": "2008",
      "unstructured": "Cornish, P.V., Ermolenko, D.N., Noller, H.F. & Ha, T. Spontaneous intersubunit rotation in single ribosomes. Mol. Cell 30, 578\u2013588 (2008).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR18",
      "doi-asserted-by": "publisher",
      "first-page": "12893",
      "DOI": "10.1073/pnas.0403884101",
      "volume": "101",
      "author": "SC Blanchard",
      "year": "2004",
      "unstructured": "Blanchard, S.C., Kim, H.D., Gonzalez, R.L. Jr., Puglisi, J.D. & Chu, S. tRNA dynamics on the ribosome during translation. Proc. Natl. Acad. Sci. USA 101, 12893\u201312898 (2004).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR19",
      "doi-asserted-by": "publisher",
      "first-page": "530",
      "DOI": "10.1016/j.jmb.2007.04.042",
      "volume": "370",
      "author": "DN Ermolenko",
      "year": "2007",
      "unstructured": "Ermolenko, D.N. et al. Observation of intersubunit movement of the ribosome in solution using FRET. J. Mol. Biol. 370, 530\u2013540 (2007).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR20",
      "doi-asserted-by": "publisher",
      "first-page": "15702",
      "DOI": "10.1073/pnas.0908077106",
      "volume": "106",
      "author": "J Fei",
      "year": "2009",
      "unstructured": "Fei, J. et al. Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation. Proc. Natl. Acad. Sci. USA 106, 15702\u201315707 (2009).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR21",
      "doi-asserted-by": "publisher",
      "first-page": "348",
      "DOI": "10.1016/j.molcel.2008.03.012",
      "volume": "30",
      "author": "J Fei",
      "year": "2008",
      "unstructured": "Fei, J., Kosuri, P., MacDougall, D.D. & Gonzalez, R.L. Jr. Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation. Mol. Cell 30, 348\u2013359 (2008).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR22",
      "doi-asserted-by": "publisher",
      "first-page": "505",
      "DOI": "10.1016/j.molcel.2007.01.022",
      "volume": "25",
      "author": "JB Munro",
      "year": "2007",
      "unstructured": "Munro, J.B., Altman, R.B., O\u2032Connor, N. & Blanchard, S.C. Identification of two distinct hybrid state intermediates on the ribosome. Mol. Cell 25, 505\u2013517 (2007).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR23",
      "doi-asserted-by": "publisher",
      "first-page": "699",
      "DOI": "10.1016/0022-2836(88)90203-3",
      "volume": "203",
      "author": "R Lill",
      "year": "1988",
      "unstructured": "Lill, R. et al. Specific recognition of the 3\u2032-terminal adenosine of tRNAPhe in the exit site of Escherichia coli ribosomes. J. Mol. Biol. 203, 699\u2013705 (1988).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR24",
      "doi-asserted-by": "publisher",
      "first-page": "369",
      "DOI": "10.1016/S0022-2836(03)00146-3",
      "volume": "327",
      "author": "SM Studer",
      "year": "2003",
      "unstructured": "Studer, S.M., Feinberg, J.S. & Joseph, S. Rapid kinetic analysis of EF-G-dependent mRNA translocation in the ribosome. J. Mol. Biol. 327, 369\u2013381 (2003).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR25",
      "doi-asserted-by": "publisher",
      "first-page": "709",
      "DOI": "10.1073/pnas.0908597107",
      "volume": "107",
      "author": "JB Munro",
      "year": "2010",
      "unstructured": "Munro, J.B. et al. Spontaneous formation of the unlocked state of the ribosome is a multistep process. Proc. Natl. Acad. Sci. USA 107, 709\u2013714 (2010).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnsmb2011_CR26",
      "doi-asserted-by": "publisher",
      "first-page": "1183",
      "DOI": "10.1016/j.jmb.2004.08.097",
      "volume": "343",
      "author": "F Peske",
      "year": "2004",
      "unstructured": "Peske, F., Savelsbergh, A., Katunin, V.I., Rodnina, M.V. & Wintermeyer, W. Conformational changes of the small ribosomal subunit during elongation factor G-dependent tRNA-mRNA translocation. J. Mol. Biol. 343, 1183\u20131194 (2004).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR27",
      "doi-asserted-by": "publisher",
      "first-page": "6772",
      "DOI": "10.1021/bi900472a",
      "volume": "48",
      "author": "X Shi",
      "year": "2009",
      "unstructured": "Shi, X., Chiu, K., Ghosh, S. & Joseph, S. Bases in 16S rRNA important for subunit association, tRNA binding, and translocation. Biochemistry 48, 6772\u20136782 (2009).",
      "journal-title": "Biochemistry"
    },
    {
      "key": "BFnsmb2011_CR28",
      "doi-asserted-by": "publisher",
      "first-page": "234",
      "DOI": "10.1038/nsmb1060",
      "volume": "13",
      "author": "S Dorner",
      "year": "2006",
      "unstructured": "Dorner, S., Brunelle, J.L., Sharma, D. & Green, R. The hybrid state of tRNA binding is an authentic translation elongation intermediate. Nat. Struct. Mol. Biol. 13, 234\u2013241 (2006).",
      "journal-title": "Nat. Struct. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR29",
      "doi-asserted-by": "publisher",
      "first-page": "1590",
      "DOI": "10.1261/rna.1076908",
      "volume": "14",
      "author": "MA Borovinskaya",
      "year": "2008",
      "unstructured": "Borovinskaya, M.A., Shoji, S., Fredrick, K. & Cate, J.H. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA 14, 1590\u20131599 (2008).",
      "journal-title": "RNA"
    },
    {
      "key": "BFnsmb2011_CR30",
      "doi-asserted-by": "publisher",
      "first-page": "545",
      "DOI": "10.1021/cb700100n",
      "volume": "2",
      "author": "MA Borovinskaya",
      "year": "2007",
      "unstructured": "Borovinskaya, M.A., Shoji, S., Holton, J.M., Fredrick, K. & Cate, J.H. A steric block in translation caused by the antibiotic spectinomycin. ACS Chem. Biol. 2, 545\u2013552 (2007).",
      "journal-title": "ACS Chem. Biol."
    },
    {
      "key": "BFnsmb2011_CR31",
      "doi-asserted-by": "publisher",
      "first-page": "9",
      "DOI": "10.1186/jbiol24",
      "volume": "4",
      "author": "AV Zavialov",
      "year": "2005",
      "unstructured": "Zavialov, A.V., Hauryliuk, V.V. & Ehrenberg, M. Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs. J. Biol. 4, 9 (2005).",
      "journal-title": "J. Biol."
    },
    {
      "key": "BFnsmb2011_CR32",
      "doi-asserted-by": "publisher",
      "first-page": "761",
      "DOI": "10.1016/S0076-6879(79)60070-8",
      "volume": "60",
      "author": "NV Belitsina",
      "year": "1979",
      "unstructured": "Belitsina, N.V., Glukhova, M.A. & Spirin, A.S. Elongation factor G-promoted translocation and polypeptide elongation in ribosomes without GTP cleavage: use of columns with matrix-bound polyuridylic acid. Methods Enzymol. 60, 761\u2013779 (1979).",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "BFnsmb2011_CR33",
      "doi-asserted-by": "publisher",
      "first-page": "437",
      "DOI": "10.1016/0006-291X(69)90934-6",
      "volume": "37",
      "author": "JW Bodley",
      "year": "1969",
      "unstructured": "Bodley, J.W., Zieve, F.J., Lin, L. & Zieve, S.T. Formation of the ribosome-G factor-GDP complex in the presence of fusidic acid. Biochem. Biophys. Res. Commun. 37, 437\u2013443 (1969).",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "BFnsmb2011_CR34",
      "doi-asserted-by": "crossref",
      "first-page": "4321",
      "DOI": "10.1016/S0021-9258(19)42519-2",
      "volume": "249",
      "author": "N Inoue-Yokosawa",
      "year": "1974",
      "unstructured": "Inoue-Yokosawa, N., Ishikawa, C. & Kaziro, Y. The role of guanosine triphosphate in translocation reaction catalyzed by elongation factor G. J. Biol. Chem. 249, 4321\u20134323 (1974).",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "BFnsmb2011_CR35",
      "doi-asserted-by": "publisher",
      "first-page": "2053",
      "DOI": "10.1038/emboj.2009.169",
      "volume": "28",
      "author": "C Ticu",
      "year": "2009",
      "unstructured": "Ticu, C., Nechifor, R., Nguyen, B., Desrosiers, M. & Wilson, K.S. Conformational changes in switch I of EF-G drive its directional cycling on and off the ribosome. EMBO J. 28, 2053\u20132065 (2009).",
      "journal-title": "EMBO J."
    },
    {
      "key": "BFnsmb2011_CR36",
      "doi-asserted-by": "publisher",
      "first-page": "772",
      "DOI": "10.1261/rna.1592509",
      "volume": "15",
      "author": "A Savelsbergh",
      "year": "2009",
      "unstructured": "Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Distinct functions of elongation factor G in ribosome recycling and translocation. RNA 15, 772\u2013780 (2009).",
      "journal-title": "RNA"
    },
    {
      "key": "BFnsmb2011_CR37",
      "doi-asserted-by": "publisher",
      "first-page": "37",
      "DOI": "10.1038/385037a0",
      "volume": "385",
      "author": "MV Rodnina",
      "year": "1997",
      "unstructured": "Rodnina, M.V., Savelsbergh, A., Katunin, V.I. & Wintermeyer, W. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome. Nature 385, 37\u201341 (1997).",
      "journal-title": "Nature"
    },
    {
      "key": "BFnsmb2011_CR38",
      "doi-asserted-by": "publisher",
      "first-page": "1517",
      "DOI": "10.1016/S1097-2765(03)00230-2",
      "volume": "11",
      "author": "A Savelsbergh",
      "year": "2003",
      "unstructured": "Savelsbergh, A. et al. An elongation factor G-induced ribosome rearrangement precedes tRNA-mRNA translocation. Mol. Cell 11, 1517\u20131523 (2003).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR39",
      "doi-asserted-by": "publisher",
      "first-page": "827",
      "DOI": "10.1126/science.1117230",
      "volume": "310",
      "author": "BS Schuwirth",
      "year": "2005",
      "unstructured": "Schuwirth, B.S. et al. Structures of the bacterial ribosome at 3.5 A resolution. Science 310, 827\u2013834 (2005).",
      "journal-title": "Science"
    },
    {
      "key": "BFnsmb2011_CR40",
      "doi-asserted-by": "publisher",
      "first-page": "865",
      "DOI": "10.1016/j.molcel.2006.08.011",
      "volume": "23",
      "author": "IK Ali",
      "year": "2006",
      "unstructured": "Ali, I.K., Lancaster, L., Feinberg, J., Joseph, S. & Noller, H.F. Deletion of a conserved, central ribosomal intersubunit RNA bridge. Mol. Cell 23, 865\u2013874 (2006).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR41",
      "doi-asserted-by": "publisher",
      "first-page": "580",
      "DOI": "10.1261/rna.2290706",
      "volume": "12",
      "author": "JS Feinberg",
      "year": "2006",
      "unstructured": "Feinberg, J.S. & Joseph, S. Ribose 2\u2032-hydroxyl groups in the 5\u2032 strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation. RNA 12, 580\u2013588 (2006).",
      "journal-title": "RNA"
    },
    {
      "key": "BFnsmb2011_CR42",
      "doi-asserted-by": "publisher",
      "first-page": "54",
      "DOI": "10.1038/nchembio.274",
      "volume": "6",
      "author": "MB Feldman",
      "year": "2010",
      "unstructured": "Feldman, M.B., Terry, D.S., Altman, R.B. & Blanchard, S.C. Aminoglycoside activity observed on single pre-translocation ribosome complexes. Nat. Chem. Biol. 6, 54\u201362 (2010).",
      "journal-title": "Nat. Chem. Biol."
    },
    {
      "key": "BFnsmb2011_CR43",
      "doi-asserted-by": "publisher",
      "first-page": "12806",
      "DOI": "10.1021/bi0264871",
      "volume": "41",
      "author": "VI Katunin",
      "year": "2002",
      "unstructured": "Katunin, V.I., Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Coupling of GTP hydrolysis by elongation factor G to translocation and factor recycling on the ribosome. Biochemistry 41, 12806\u201312812 (2002).",
      "journal-title": "Biochemistry"
    },
    {
      "key": "BFnsmb2011_CR44",
      "doi-asserted-by": "publisher",
      "first-page": "351",
      "DOI": "10.1016/0022-2836(86)90370-0",
      "volume": "192",
      "author": "JM Robertson",
      "year": "1986",
      "unstructured": "Robertson, J.M., Paulsen, H. & Wintermeyer, W. Pre-steady-state kinetics of ribosomal translocation. J. Mol. Biol. 192, 351\u2013360 (1986).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR45",
      "doi-asserted-by": "publisher",
      "first-page": "694",
      "DOI": "10.1126/science.1179709",
      "volume": "326",
      "author": "YG Gao",
      "year": "2009",
      "unstructured": "Gao, Y.G. et al. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 326, 694\u2013699 (2009).",
      "journal-title": "Science"
    },
    {
      "key": "BFnsmb2011_CR46",
      "doi-asserted-by": "publisher",
      "first-page": "205",
      "DOI": "10.1016/S0014-5793(98)00982-X",
      "volume": "434",
      "author": "KA Martemyanov",
      "year": "1998",
      "unstructured": "Martemyanov, K.A., Yarunin, A.S., Liljas, A. & Gudkov, A.T. An intact conformation at the tip of elongation factor G domain IV is functionally important. FEBS Lett. 434, 205\u2013208 (1998).",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "BFnsmb2011_CR47",
      "doi-asserted-by": "publisher",
      "first-page": "501",
      "DOI": "10.1016/S1097-2765(00)00049-6",
      "volume": "6",
      "author": "F Peske",
      "year": "2000",
      "unstructured": "Peske, F., Matassova, N.B., Savelsbergh, A., Rodnina, M.V. & Wintermeyer, W. Conformationally restricted elongation factor G retains GTPase activity but is inactive in translocation on the ribosome. Mol. Cell 6, 501\u2013505 (2000).",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "BFnsmb2011_CR48",
      "doi-asserted-by": "publisher",
      "first-page": "131",
      "DOI": "10.1016/S0092-8674(00)80905-8",
      "volume": "92",
      "author": "KS Wilson",
      "year": "1998",
      "unstructured": "Wilson, K.S. & Noller, H.F. Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing. Cell 92, 131\u2013139 (1998).",
      "journal-title": "Cell"
    },
    {
      "key": "BFnsmb2011_CR49",
      "doi-asserted-by": "publisher",
      "first-page": "832",
      "DOI": "10.1017/S1355838299990714",
      "volume": "5",
      "author": "GM Culver",
      "year": "1999",
      "unstructured": "Culver, G.M. & Noller, H.F. Efficient reconstitution of functional Escherichia coli 30S ribosomal subunits from a complete set of recombinant small subunit ribosomal proteins. RNA 5, 832\u2013843 (1999).",
      "journal-title": "RNA"
    },
    {
      "key": "BFnsmb2011_CR50",
      "doi-asserted-by": "publisher",
      "first-page": "459",
      "DOI": "10.1016/j.jmb.2005.09.010",
      "volume": "354",
      "author": "R Hickerson",
      "year": "2005",
      "unstructured": "Hickerson, R., Majumdar, Z.K., Baucom, A., Clegg, R.M. & Noller, H.F. Measurement of internal movements within the 30 S ribosomal subunit using Forster resonance energy transfer. J. Mol. Biol. 354, 459\u2013472 (2005).",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "BFnsmb2011_CR51",
      "doi-asserted-by": "publisher",
      "first-page": "1129",
      "DOI": "10.1006/jmbi.2000.3621",
      "volume": "297",
      "author": "KR Lieberman",
      "year": "2000",
      "unstructured": "Lieberman, K.R. et al. The 23 S rRNA environment of ribosomal protein L9 in the 50 S ribosomal subunit. J. Mol. Biol. 297, 1129\u20131143 (2000).",
      "journal-title": "J. Mol. Biol."
    }
  ],
  "container-title": "Nature Structural &amp; Molecular Biology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://www.nature.com/articles/nsmb.2011.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://www.nature.com/articles/nsmb.2011",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://www.nature.com/articles/nsmb.2011.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        5,
        19
      ]
    ],
    "date-time": "2023-05-19T04:29:05Z",
    "timestamp": 1684470545000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.nature.com/articles/nsmb.2011"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2011,
        3,
        13
      ]
    ]
  },
  "references-count": 51,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2011,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "BFnsmb2011"
  ],
  "URL": "http://dx.doi.org/10.1038/nsmb.2011",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.9653957.10325055",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "1545-9993",
    "1545-9985"
  ],
  "subject": [],
  "container-title-short": "Nat Struct Mol Biol",
  "published": {
    "date-parts": [
      [
        2011,
        3,
        13
      ]
    ]
  }
}