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Bibliography

Warkocki, Z., Odenwälder, P., Schmitzová, J., Platzmann, F., Stark, H., Urlaub, H., Ficner, R., Fabrizio, P., & Lührmann, R. (2009). Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Nature Structural & Molecular Biology, 16(12), 1237–1243.

Authors 9
  1. Zbigniew Warkocki (first)
  2. Peter Odenwälder (additional)
  3. Jana Schmitzová (additional)
  4. Florian Platzmann (additional)
  5. Holger Stark (additional)
  6. Henning Urlaub (additional)
  7. Ralf Ficner (additional)
  8. Patrizia Fabrizio (additional)
  9. Reinhard Lührmann (additional)
References 43 Referenced 149
  1. Will, C.L. & Lührmann, R. Spliceosome structure and function. in RNA World (eds. Gesteland, R.F., Cech, T.R. & Atkins, J.F.) 369–400 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2006).
  2. Staley, J.P. & Guthrie, C. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92, 315–326 (1998). (10.1016/S0092-8674(00)80925-3) / Cell by JP Staley (1998)
  3. Nilsen, T.W. RNA-RNA interactions in nuclear pre-mRNA splicing. in RNA Structure and Function (eds. Grundber-Manago, M., & Simons, R.W.) 279–307 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1998).
  4. Nilsen, T.W. The spliceosome: the most complex macromolecular machine in the cell? Bioessays 25, 1147–1149 (2003). (10.1002/bies.10394) / Bioessays by TW Nilsen (2003)
  5. Pyle, A.M. Translocation and unwinding mechanisms of RNA and DNA helicases. Annu. Rev. Biophys. 37, 317–336 (2008). (10.1146/annurev.biophys.37.032807.125908) / Annu. Rev. Biophys. by AM Pyle (2008)
  6. Schwer, B. & Guthrie, C. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349, 494–499 (1991). (10.1038/349494a0) / Nature by B Schwer (1991)
  7. Teigelkamp, S., McGarvey, M., Plumpton, M. & Beggs, J.D. The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. EMBO J. 13, 888–897 (1994). (10.1002/j.1460-2075.1994.tb06332.x) / EMBO J. by S Teigelkamp (1994)
  8. King, D.S. & Beggs, J.D. Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae. Nucleic Acids Res. 18, 6559–6564 (1990). (10.1093/nar/18.22.6559) / Nucleic Acids Res. by DS King (1990)
  9. Schwer, B. & Gross, C.H. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J. 17, 2086–2094 (1998). (10.1093/emboj/17.7.2086) / EMBO J. by B Schwer (1998)
  10. Kim, S.H. & Lin, R.J. Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol. Cell. Biol. 16, 6810–6819 (1996). (10.1128/MCB.16.12.6810) / Mol. Cell. Biol. by SH Kim (1996)
  11. Fabrizio, P. et al. The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. Mol. Cell (in the press).
  12. Burgess, S.M. & Guthrie, C. A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates. Cell 73, 1377–1391 (1993). (10.1016/0092-8674(93)90363-U) / Cell by SM Burgess (1993)
  13. Mefford, M.A. & Staley, J.P. Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps. RNA 15, 1386–1397 (2009). (10.1261/rna.1582609) / RNA by MA Mefford (2009)
  14. Vijayraghavan, U. & Abelson, J. PRP18, a protein required for the second reaction in pre-mRNA splicing. Mol. Cell. Biol. 10, 324–332 (1990). (10.1128/MCB.10.1.324) / Mol. Cell. Biol. by U Vijayraghavan (1990)
  15. Horowitz, D.S. & Abelson, J. Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent. Genes Dev. 7, 320–329 (1993). (10.1101/gad.7.2.320) / Genes Dev. by DS Horowitz (1993)
  16. Umen, J.G. & Guthrie, C. The second catalytic step of pre-mRNA splicing. RNA 1, 869–885 (1995). / RNA by JG Umen (1995)
  17. Ansari, A. & Schwer, B. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J. 14, 4001–4009 (1995). (10.1002/j.1460-2075.1995.tb00071.x) / EMBO J. by A Ansari (1995)
  18. Brys, A. & Schwer, B. Requirement for SLU7 in yeast pre-mRNA splicing is dictated by the distance between the branchpoint and the 3′ splice site. RNA 2, 707–717 (1996). / RNA by A Brys (1996)
  19. James, S.A., Turner, W. & Schwer, B. How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8, 1068–1077 (2002). (10.1017/S1355838202022033) / RNA by SA James (2002)
  20. Wahl, M.C., Will, C.L. & Lührmann, R. The spliceosome: design principles of a dynamic RNP machine. Cell 136, 701–718 (2009). (10.1016/j.cell.2009.02.009) / Cell by MC Wahl (2009)
  21. Tseng, C.K. & Cheng, S.C. Both catalytic steps of nuclear pre-mRNA splicing are reversible. Science 320, 1782–1784 (2008). (10.1126/science.1158993) / Science by CK Tseng (2008)
  22. Deckert, J. et al. Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions. Mol. Cell. Biol. 26, 5528–5543 (2006). (10.1128/MCB.00582-06) / Mol. Cell. Biol. by J Deckert (2006)
  23. Bessonov, S., Anokhina, M., Will, C.L., Urlaub, H. & Lührmann, R. Isolation of an active step I spliceosome and composition of its RNP core. Nature 452, 846–850 (2008). (10.1038/nature06842) / Nature by S Bessonov (2008)
  24. Makarov, E.M. et al. Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome. Science 298, 2205–2208 (2002). (10.1126/science.1077783) / Science by EM Makarov (2002)
  25. Chan, S.P., Kao, D.I., Tsai, W.Y. & Cheng, S.C. The Prp19p-associated complex in spliceosome activation. Science 302, 279–282 (2003). (10.1126/science.1086602) / Science by SP Chan (2003)
  26. Roy, J., Kim, K., Maddock, J.R., Anthony, J.G. & Woolford, J.L. Jr. The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing. RNA 1, 375–390 (1995). / RNA by J Roy (1995)
  27. Liu, Y.C., Chen, H.C., Wu, N.Y. & Cheng, S.C. A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol. Cell. Biol. 27, 5403–5413 (2007). (10.1128/MCB.00346-07) / Mol. Cell. Biol. by YC Liu (2007)
  28. Sapra, A.K., Khandelia, P. & Vijayraghavan, U. The splicing factor Prp17 interacts with the U2, U5 and U6 snRNPs and associates with the spliceosome pre- and post-catalysis. Biochem. J. 416, 365–374 (2008). (10.1042/BJ20081195) / Biochem. J. by AK Sapra (2008)
  29. Ohi, M.D. et al. Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol. Cell. Biol. 22, 2011–2024 (2002). (10.1128/MCB.22.7.2011-2024.2002) / Mol. Cell. Biol. by MD Ohi (2002)
  30. Burkhard, P., Stetefeld, J. & Strelkov, S.V. Coiled coils: a highly versatile protein folding motif. Trends Cell Biol. 11, 82–88 (2001). (10.1016/S0962-8924(00)01898-5) / Trends Cell Biol. by P Burkhard (2001)
  31. Farias, S.T. & Bonato, M.C. Preferred amino acids and thermostability. Genet. Mol. Res. 2, 383–393 (2003). / Genet. Mol. Res. by ST Farias (2003)
  32. Farias, S.T., van der Linden, M.G., Rego, T.G., Araujo, D.A. & Bonato, M.C. Thermo-search: lifestyle and thermostability analysis. In Silico Biol. 4, 377–380 (2004). (10.3233/ISB-00140) / In Silico Biol. by ST Farias (2004)
  33. Sander, B., Golas, M.M. & Stark, H. Corrim-based alignment for improved speed in single-particle image processing. J. Struct. Biol. 143, 219–228 (2003). (10.1016/j.jsb.2003.08.001) / J. Struct. Biol. by B Sander (2003)
  34. van Heel, M. & Frank, J. Use of multivariate statistics in analysing the images of biological macromolecules. Ultramicroscopy 6, 187–194 (1981). / Ultramicroscopy by M van Heel (1981)
  35. Chiu, Y.F. et al. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol. Cell. Biol. 29, 5671–5678 (2009). (10.1128/MCB.00773-09) / Mol. Cell. Biol. by YF Chiu (2009)
  36. Gozani, O., Potashkin, J. & Reed, R. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site. Mol. Cell. Biol. 18, 4752–4760 (1998). (10.1128/MCB.18.8.4752) / Mol. Cell. Biol. by O Gozani (1998)
  37. Smith, D.J., Query, C.C. & Konarska, M.M. trans-splicing to spliceosomal U2 snRNA suggests disruption of branch site-U2 pairing during pre-mRNA splicing. Mol. Cell 26, 883–890 (2007). (10.1016/j.molcel.2007.05.020) / Mol. Cell by DJ Smith (2007)
  38. Yean, S.L. & Lin, R.J. U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol. Cell. Biol. 11, 5571–5577 (1991). (10.1128/MCB.11.11.5571) / Mol. Cell. Biol. by SL Yean (1991)
  39. Fabrizio, P., McPheeters, D.S. & Abelson, J. In vitro assembly of yeast U6 snRNP: a functional assay. Genes Dev. 3, 2137–2150 (1989). (10.1101/gad.3.12b.2137) / Genes Dev. by P Fabrizio (1989)
  40. Kastner, B. et al. GraFix: sample preparation for single-particle electron cryomicroscopy. Nat. Methods 5, 53–55 (2008). (10.1038/nmeth1139) / Nat. Methods by B Kastner (2008)
  41. Shevchenko, A., Wilm, M., Vorm, O. & Mann, M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850–858 (1996). (10.1021/ac950914h) / Anal. Chem. by A Shevchenko (1996)
  42. Hu, Q. et al. The Orbitrap: a new mass spectrometer. J. Mass Spectrom. 40, 430–443 (2005). (10.1002/jms.856) / J. Mass Spectrom. by Q Hu (2005)
  43. Studier, F.W. Protein production by auto-induction in high-density shaking cultures. Protein Expr. Purif. 41, 207–234 (2005). (10.1016/j.pep.2005.01.016) / Protein Expr. Purif. by FW Studier (2005)
Dates
Type When
Created 15 years, 9 months ago (Nov. 23, 2009, 5:10 a.m.)
Deposited 6 months, 1 week ago (Feb. 13, 2025, 6:01 a.m.)
Indexed 1 month, 3 weeks ago (July 4, 2025, 12:03 p.m.)
Issued 15 years, 9 months ago (Nov. 22, 2009)
Published 15 years, 9 months ago (Nov. 22, 2009)
Published Online 15 years, 9 months ago (Nov. 22, 2009)
Published Print 15 years, 8 months ago (Dec. 1, 2009)
Funders 0

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@article{Warkocki_2009, title={Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components}, volume={16}, ISSN={1545-9985}, url={http://dx.doi.org/10.1038/nsmb.1729}, DOI={10.1038/nsmb.1729}, number={12}, journal={Nature Structural & Molecular Biology}, publisher={Springer Science and Business Media LLC}, author={Warkocki, Zbigniew and Odenwälder, Peter and Schmitzová, Jana and Platzmann, Florian and Stark, Holger and Urlaub, Henning and Ficner, Ralf and Fabrizio, Patrizia and Lührmann, Reinhard}, year={2009}, month=nov, pages={1237–1243} }