Crossref
journal-article
Springer Science and Business Media LLC
Nature Reviews Molecular Cell Biology (297)
References
111
Referenced
314
-
Wickner, W. & Leonard, M. R. Escherichia coli preprotein translocase. J. Biol. Chem. 271, 29514–29516 (1996).
(
10.1074/jbc.271.47.29514
) / J. Biol. Chem. by W Wickner (1996) -
Schatz, G. & Dobberstein, B. Common principles of protein translocation across membranes. Science 271, 1519–1526 (1996).
(
10.1126/science.271.5255.1519
) / Science by G Schatz (1996) -
Neupert, W. Protein import into mitochondria. Annu. Rev. Biochem. 66, 863–917 (1997).
(
10.1146/annurev.biochem.66.1.863
) / Annu. Rev. Biochem. by W Neupert (1997) -
Matlack, K. E. S., Mothes, W. & Rapoport, T. A. Protein translocation: tunnel vision. Cell 92, 381–390 (1998).
(
10.1016/S0092-8674(00)80930-7
) / Cell by KES Matlack (1998) -
Keegstra, K. & Froehlich, J. E. Protein import into chloroplasts. Curr. Opin. Plant Biol. 2, 471–476 (1999).
(
10.1016/S1369-5266(99)00021-7
) / Curr. Opin. Plant Biol. by K Keegstra (1999) -
Johnson, A. E. & Haigh, N. G. The ER translocon and retrotranslocation: is the shift into reverse manual or automatic? Cell 102, 709–712 (2000).
(
10.1016/S0092-8674(00)00059-3
) / Cell by AE Johnson (2000) -
Rassow, J. & Pfanner, N. The protein import machinery of the mitochondrial membranes. Traffic 1, 457–464 (2000).
(
10.1034/j.1600-0854.2000.010603.x
) / Traffic by J Rassow (2000) -
Driessen, A. J., Manting, E. H. & van der Does, C. The structural basis of protein targeting and translocation in bacteria. Nature Struct. Biol. 8, 492–498 (2001).
(
10.1038/88549
) / Nature Struct. Biol. by AJ Driessen (2001) -
Holroyd, C. & Erdmann, R. Protein translocation machineries of peroxisomes. FEBS Lett. 501, 6–10 (2001).
(
10.1016/S0014-5793(01)02617-5
) / FEBS Lett. by C Holroyd (2001) -
Robinson, C., Thompson, S. J. & Woolhead, C. Multiple pathways used for the targeting of thylakoid proteins in chloroplasts. Traffic 2, 245–251 (2001).
(
10.1034/j.1600-0854.2001.1r010.x
) / Traffic by C Robinson (2001) -
Pfanner, N., Craig, E. A. & Hönlinger, A. Mitochondrial preprotein translocase. Annu. Rev. Cell Dev. Biol. 13, 25–51 (1997).
(
10.1146/annurev.cellbio.13.1.25
) / Annu. Rev. Cell Dev. Biol. by N Pfanner (1997) -
Rapoport, T. A., Matlack, K. E. S., Plath, K., Misselwitz, B. & Staeck, O. Posttranslational protein translocation across the membrane of the endoplasmic reticulum. Biol. Chem. 380, 1143–1150 (1999).
(
10.1515/BC.1999.145
) / Biol. Chem. by TA Rapoport (1999) -
Bauer, M. F., Hofmann, S., Neupert, W. & Brunner, M. Protein translocation into mitochondria: the role of TIM complexes. Trends Cell Biol. 10, 25–31 (2000).
(
10.1016/S0962-8924(99)01684-0
) / Trends Cell Biol. by MF Bauer (2000) -
Paschen, S. A. & Neupert, W. Protein import into mitochondria. IUBMB Life 52, 101–112 (2001).
(
10.1080/15216540152845894
) / IUBMB Life by SA Paschen (2001) -
Pfanner, N. & Geissler, A. Versatility of the mitochondrial protein import machinery. Nature Rev. Mol. Cell Biol. 2, 339–349 (2001).
(
10.1038/35073006
) / Nature Rev. Mol. Cell Biol. by N Pfanner (2001) -
Berthold, J. et al. The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81, 1085–1093 (1995).
(
10.1016/S0092-8674(05)80013-3
) / Cell by J Berthold (1995) -
Moro, F., Sirrenberg, C., Schneider, H.-C., Neupert, W. & Brunner, M. The TIM17·23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. EMBO J. 18, 3667–3675 (1999).Tim44 is shown to be a dimer that recruits two molecules of mtHsp70 to the import site. A 'hand-over-hand' mode of protein import is proposed.
(
10.1093/emboj/18.13.3667
) / EMBO J. by F Moro (1999) -
Milisav, I., Moro, F., Neupert, W. & Brunner, M. Modular structure of the TIM23 preprotein translocase of mitochondria. J. Biol. Chem. 276, 25856–25861 (2001).
(
10.1074/jbc.M102132200
) / J. Biol. Chem. by I Milisav (2001) -
Sirrenberg, C., Bauer, M. F., Guiard, B., Neupert, W. & Brunner, M. Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22. Nature 384, 582–585 (1996).
(
10.1038/384582a0
) / Nature by C Sirrenberg (1996) -
Sirrenberg, C. et al. Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature 391, 912–915 (1998).
(
10.1038/36136
) / Nature by C Sirrenberg (1998) -
Kerscher, O., Holder, J., Srinivasan, M., Leung, R. S. & Jensen, R. E. The Tim54p–Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J. Cell Biol. 139, 1663–1675 (1997).
(
10.1083/jcb.139.7.1663
) / J. Cell Biol. by O Kerscher (1997) -
Kerscher, O., Sepuri, N. B. & Jensen, R. E. Tim18p is a new component of the Tim54p–Tim22p translocon in the mitochondrial inner membrane. Mol. Biol. Cell 11, 103–116 (2000).
(
10.1091/mbc.11.1.103
) / Mol. Biol. Cell by O Kerscher (2000) -
Koehler, C. M. et al. Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science. 279, 369–373 (1998).
(
10.1126/science.279.5349.369
) / Science. by CM Koehler (1998) -
Koehler, C. M. et al. Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane. Mol. Cell. Biol. 20, 1187–1193 (2000).
(
10.1128/MCB.20.4.1187-1193.2000
) / Mol. Cell. Biol. by CM Koehler (2000) -
Paschen, S. A. et al. The role of the TIM8–13 complex in the import of Tim23 into mitochondria. EMBO J. 19, 6392–6400 (2000).
(
10.1093/emboj/19.23.6392
) / EMBO J. by SA Paschen (2000) -
Kovermann, P. et al. Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel. Mol. Cell. 9, 363–373 (2002).
(
10.1016/S1097-2765(02)00446-X
) / Mol. Cell. by P Kovermann (2002) -
Bauer, M. F., Sirrenberg, C., Neupert, W. & Brunner, M. Role of Tim23 as voltage sensor and presequence receptor in protein import into mitochondria. Cell 87, 33–41 (1996).
(
10.1016/S0092-8674(00)81320-3
) / Cell by MF Bauer (1996) -
Mayer, A., Neupert, W. & Lill, R. Mitochondrial protein import: reversible binding of the presequence at the trans side of the outer membrane drives partial translocation and unfolding. Cell 80, 127–137 (1995).
(
10.1016/0092-8674(95)90457-3
) / Cell by A Mayer (1995) -
Hill, K. et al. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395, 516–521 (1998).
(
10.1038/26780
) / Nature by K Hill (1998) -
Stan, T. et al. Recognition of preproteins by the isolated TOM complex of mitochondria. EMBO J. 19, 4895–4902 (2000).
(
10.1093/emboj/19.18.4895
) / EMBO J. by T Stan (2000) -
Ahting, U. et al. Tom40, the pore-forming component of the protein-conducting TOM channel in the outer membrane of mitochondria. J. Cell Biol. 153, 1151–1160 (2001).
(
10.1083/jcb.153.6.1151
) / J. Cell Biol. by U Ahting (2001) -
Model, K. et al. Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex. J. Mol. Biol. 316, 657–666 (2002).
(
10.1006/jmbi.2001.5365
) / J. Mol. Biol. by K Model (2002) -
Kanamori, T., Nishikawa, S., Shin, I., Schultz, P. G. & Endo, T. Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking. Proc. Natl Acad. Sci. USA 94, 485–490 (1997).
(
10.1073/pnas.94.2.485
) / Proc. Natl Acad. Sci. USA by T Kanamori (1997) -
Kanamori, T. et al. Uncoupling of transfer of the presequence and unfolding of the mature domain in precursor translocation across the mitochondrial outer membrane. Proc. Natl Acad. Sci. USA 96, 3634–3639 (1999).
(
10.1073/pnas.96.7.3634
) / Proc. Natl Acad. Sci. USA by T Kanamori (1999) -
Fölsch, H., Gaume, B., Brunner, M., Neupert, W. & Stuart, R. A. C- to N-terminal translocation of preproteins into mitochondria. EMBO J. 17, 6508–6515 (1998).
(
10.1093/emboj/17.22.6508
) / EMBO J. by H Fölsch (1998) -
Donzeau, M. et al. Tim23 links the inner and outer mitochondrial membranes. Cell 101, 401–412 (2000).
(
10.1016/S0092-8674(00)80850-8
) / Cell by M Donzeau (2000) -
Truscott, K. N. et al. A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23. Nature Struct. Biol. 8, 1074–1082 (2001).
(
10.1038/nsb726
) / Nature Struct. Biol. by KN Truscott (2001) -
Rassow, J., Hartl, F.-U., Guiard, B., Pfanner, N. & Neupert, W. Polypeptides traverse the mitochondrial envelope in an extended state. FEBS Lett. 275, 190–194 (1990).
(
10.1016/0014-5793(90)81469-5
) / FEBS Lett. by J Rassow (1990) -
Künkele, K. P. et al. The preprotein translocation channel of the outer membrane of mitochondria. Cell 93, 1009–1019 (1998).
(
10.1016/S0092-8674(00)81206-4
) / Cell by KP Künkele (1998) -
Schwartz, M. P., Huang, S. & Matouschek, A. The structure of precursor proteins during import into mitochondria. J. Biol. Chem. 274, 12759–12764 (1999).
(
10.1074/jbc.274.18.12759
) / J. Biol. Chem. by MP Schwartz (1999) -
Schwartz, M. P. & Matouschek, A. The dimensions of the protein import channels in the outer and inner mitochondrial membranes. Proc. Natl Acad. Sci. USA 96, 13086–13090 (1999).
(
10.1073/pnas.96.23.13086
) / Proc. Natl Acad. Sci. USA by MP Schwartz (1999) -
Martin, J., Malke, K. & Pfanner, N. Role of energized inner mitochondrial membrane in mitochondrial protein import: Δψ drives the movement of the presequence. J. Biol. Chem. 266, 18051–18057 (1991).
(
10.1016/S0021-9258(18)55235-2
) / J. Biol. Chem. by J Martin (1991) -
Ungermann, C., Neupert, W. & Cyr, D. M. The role of Hsp70 in conferring unidirectionality on protein translocation into mitochondria. Science 266, 1250–1253 (1994).
(
10.1126/science.7973708
) / Science by C Ungermann (1994) -
Ungermann, C., Guiard, B., Neupert, W. & Cyr, D. M. The Δψ- and Hsp70/MIM44-dependent reaction cycle driving early steps of protein import into mitochondria. EMBO J. 15, 735–744 (1996).
(
10.1002/j.1460-2075.1996.tb00409.x
) / EMBO J. by C Ungermann (1996) -
Herrmann, J. M. & Neupert, W. What fuels polypeptide translocation? An energetical view on mitochondrial protein sorting. Biochim. Biophys. Acta 1459, 331–338 (2000).
(
10.1016/S0005-2728(00)00169-9
) / Biochim. Biophys. Acta by JM Herrmann (2000) -
Geissler, A., Rassow, J., Pfanner, N. & Voos, W. Mitochondrial import driving forces: enhanced trapping by matrix hsp70 stimulates translocation and reduces the membrane potential dependence of loosely folded preproteins. Mol. Cell. Biol. 21, 7097–7104 (2001).
(
10.1128/MCB.21.20.7097-7104.2001
) / Mol. Cell. Biol. by A Geissler (2001) -
Huang, S., Ratliff, K. S. & Matouschek, A. Protein unfolding by the mitochondrial membrane potential. Nature Struct. Biol. 9, 301–307 (2002).
(
10.1038/nsb772
) / Nature Struct. Biol. by S Huang (2002) -
Maarse, A. C., Blom, J., Grivell, L. A. & Meijer, M. MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria. EMBO J. 11, 3619–3628 (1992).
(
10.1002/j.1460-2075.1992.tb05446.x
) / EMBO J. by AC Maarse (1992) -
Scherer, P. E., Manning-Krieg, U. C., Jeno, P., Schatz, G. & Horst, M. Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane. Proc. Natl Acad. Sci. USA 89, 11930–11934 (1992).
(
10.1073/pnas.89.24.11930
) / Proc. Natl Acad. Sci. USA by PE Scherer (1992) -
Schneider, H.-C. et al. Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature 371, 768–774 (1994).
(
10.1038/371768a0
) / Nature by H-C Schneider (1994) -
Craig, E. A. et al. SSC1, an essential member of the yeast HSP70 multigene family, encodes a mitochondrial protein. Mol. Cell. Biol. 9, 3000–3008 (1989).
(
10.1128/MCB.9.7.3000
) / Mol. Cell. Biol. by EA Craig (1989) -
Kang, P.-J. et al. Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature 348, 137–143 (1990).
(
10.1038/348137a0
) / Nature by P-J Kang (1990) -
Rassow, J. et al. Mitochondrial protein import: biochemical and genetic evidence for interaction of matrix hsp70 and the inner membrane protein MIM44. J. Cell Biol. 127, 1547–1556 (1994).
(
10.1083/jcb.127.6.1547
) / J. Cell Biol. by J Rassow (1994) -
Kronidou, N. G. et al. Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane. Proc. Natl Acad. Sci. USA 91, 12818–12822 (1994).References 50, 53 and 54 show that Tim44 recruits mtHsp70 in a nucleotide-specific manner to the import site. Tim44 is a receptor, but not a substrate, for mtHsp70.
(
10.1073/pnas.91.26.12818
) / Proc. Natl Acad. Sci. USA by NG Kronidou (1994) -
Bömer, U. et al. Separation of structural and dynamic functions of the mitochondrial translocase: Tim44 is crucial for the inner membrane import sites in translocation of tightly folded domains, but not of loosely folded preproteins. EMBO J. 17, 4226–4237 (1998).
(
10.1093/emboj/17.15.4226
) / EMBO J. by U Bömer (1998) -
Schneider, H.-C., Westermann, B., Neupert, W. & Brunner, M. The nucleotide exchange factor MGE exerts a key function in the ATP-dependent cycle of mt-Hsp70–Tim44 interaction driving mitochondrial protein import. EMBO J. 15, 5796–5803 (1996).
(
10.1002/j.1460-2075.1996.tb00966.x
) / EMBO J. by H-C Schneider (1996) -
Gragerov, A., Zeng, L., Zhao, X., Burkholder, W. & Gottesman, M. E. Specificity of DnaK–peptide binding. J. Mol. Biol. 235, 848–854 (1994).
(
10.1006/jmbi.1994.1043
) / J. Mol. Biol. by A Gragerov (1994) -
Bukau, B. & Horwich, A. L. The Hsp70 and Hsp60 chaperone machines. Cell 58, 351–366 (1998).
(
10.1016/S0092-8674(00)80928-9
) / Cell by B Bukau (1998) -
Rüdiger, S., Buchberger, A. & Bukau, B. Interaction of Hsp70 chaperones with substrates. Nature Struct. Biol. 92, 342–349 (1997).
(
10.1038/nsb0597-342
) / Nature Struct. Biol. by S Rüdiger (1997) -
Rüdiger, S., Germeroth, L., Schneider-Mergener, J. & Bukau, B. Substrate specificity of the DnaK chaperone determined by screening cellulose-bound peptide libraries. EMBO J. 16, 1501–1507 (1997).
(
10.1093/emboj/16.7.1501
) / EMBO J. by S Rüdiger (1997) -
Mayer, M. P., Rüdiger, S. & Bukau, B. Molecular basis for interactions of the DnaK chaperone with substrates. Biol. Chem. 381, 877–885 (2000).
(
10.1515/BC.2000.109
) / Biol. Chem. by MP Mayer (2000) -
Mayer, M. P. et al. Multistep mechanism of substrate binding determines chaperone activity of Hsp70. Nature Struct. Biol. 7, 586–593 (2000).
(
10.1038/76819
) / Nature Struct. Biol. by MP Mayer (2000) -
Laloraya, S., Gambill, B. D. & Craig, E. A. A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation. Proc. Natl Acad. Sci. USA 91, 6481–6485 (1994).
(
10.1073/pnas.91.14.6481
) / Proc. Natl Acad. Sci. USA by S Laloraya (1994) -
Laloraya, S., Dekker, P. J. T., Voos, W., Craig, E. A. & Pfanner, N. Mitochondrial GrpE modulates the function of matrix Hsp70 in translocation and maturation of preproteins. Mol. Cell. Biol. 15, 7098–7105 (1995).
(
10.1128/MCB.15.12.7098
) / Mol. Cell. Biol. by S Laloraya (1995) -
Westermann, B., Prip-Buus, C., Neupert, W. & Schwarz, E. The role of the GrpE homologue, Mge1p, in mediating protein import and protein folding in mitochondria. EMBO J. 14, 3452–3460 (1995).
(
10.1002/j.1460-2075.1995.tb07351.x
) / EMBO J. by B Westermann (1995) -
Dekker, P. J. T. & Pfanner, N. Role of mitochondrial GrpE and phosphate in the ATPase cycle of matrix Hsp70. J. Mol. Biol. 270, 321–327 (1997).
(
10.1006/jmbi.1997.1131
) / J. Mol. Biol. by PJT Dekker (1997) -
Bolliger, L. et al. A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability. EMBO J. 13, 1998–2006 (1994).
(
10.1002/j.1460-2075.1994.tb06469.x
) / EMBO J. by L Bolliger (1994) -
Ikeda, E., Yoshida, S., Mitsuzawa, H., Uno, I. & Toh-e, A. YGE1 is a yeast homologue of Escherichia coli grpE and is required for maintenance of mitochondrial functions. FEBS Lett. 339, 265–268 (1994).
(
10.1016/0014-5793(94)80428-1
) / FEBS Lett. by E Ikeda (1994) -
Nakai, M., Kato, Y., Ikeda, E., Toh-e, A. & Endo, T. Yge1p, a eukaryotic Grp-E homolog, is localized in the mitochondrial matrix and interacts with mitochondrial Hsp70. Biochem. Biophys. Res. Commun. 200, 435–442 (1994).
(
10.1006/bbrc.1994.1468
) / Biochem. Biophys. Res. Commun. by M Nakai (1994) -
Neupert, W., Hartl, F. U., Craig E. A. & Pfanner, N. How do polypeptides cross the mitochondrial membranes? Cell 63, 447–450 (1990).
(
10.1016/0092-8674(90)90437-J
) / Cell by W Neupert (1990) -
Simon, S. M., Peskin, C. S. & Oster, G. F. What drives the translocation of proteins? Proc. Natl Acad. Sci. USA 89, 3770–3774 (1992).
(
10.1073/pnas.89.9.3770
) / Proc. Natl Acad. Sci. USA by SM Simon (1992) -
Simon, S. M. & Blobel, G. Mechanisms of translocation of proteins across membranes. Subcell. Biochem. 21, 1–15 (1993).
(
10.1007/978-1-4615-2912-5_1
) / Subcell. Biochem. by SM Simon (1993) -
Glick, B. S., Wachter, C., Reid, G. A. & Schatz, G. Import of cytochrome b 2 into the mitochondrial intermembrane space: the tightly folded heme-binding domain makes import dependent on matrix ATP. Protein Sci. 2, 1901–1917 (1993).
(
10.1002/pro.5560021112
) / Protein Sci. by BS Glick (1993) -
Glick, B. S. Can Hsp70 proteins act as force-generating motors? Cell 80, 11–14 (1995).This describes the concept of mtHsp70 acting as part of an import motor by exerting a pulling force on preproteins.
(
10.1016/0092-8674(95)90444-1
) / Cell by BS Glick (1995) -
Moro, F., Okamoto, K., Donzeau, M., Neupert, W. & Brunner, M. Mitochondrial protein import: molecular basis of the ATP-dependent interaction of MtHsp70 with Tim44. J. Biol. Chem. 277, 6874–6880 (2002).
(
10.1074/jbc.M107935200
) / J. Biol. Chem. by F Moro (2002) -
Okamoto, K. et al. The protein import motor of mitochondria: a targeted molecular ratchet driving unfolding and translocation. EMBO J. 21, 3659–3671 (2002).This study provides several lines of evidence that mtHsp70 promotes unfolding of folded precursors by harvesting spontaneous local unfolding events. The import motor seems to act by a Brownian-ratchet mechanism. MtHsp70 drives unfolding of DHFR and titin domains, even if they are preceded by stretches of polyglutamate to which mtHsp70 cannot bind in order to pull.
(
10.1093/emboj/cdf358
) / EMBO J. by K Okamoto (2002) -
Chauwin, J. F., Oster, G. & Glick, B. S. Strong precursor–pore interactions constrain models for mitochondrial protein import. Biophys J. 74, 1732–1743 (1998).
(
10.1016/S0006-3495(98)77884-1
) / Biophys J. by JF Chauwin (1998) -
Pfanner, N. & Meijer, M. Pulling in the proteins. Curr. Biol. 5, 132–135 (1995).
(
10.1016/S0960-9822(95)00033-9
) / Curr. Biol. by N Pfanner (1995) -
Jensen, R. E. & Johnson, A. E. Protein translocation: is Hsp70 pulling my chain? Curr. Biol. 9, R779–R782 (1999).
(
10.1016/S0960-9822(00)80012-3
) / Curr. Biol. by RE Jensen (1999) -
Ryan, M. T. & Pfanner, N. Hsp70 proteins in protein translocation. Adv. Protein Chem. 59, 223–242 (2001).
(
10.1016/S0065-3233(01)59007-5
) / Adv. Protein Chem. by MT Ryan (2001) -
Pfanner, N. T. & Geissler, A. Versatility of the mitochondrial protein import machinery. Nature Rev. Mol. Cell Biol. 2, 339–349 (2001).
(
10.1038/35073006
) / Nature Rev. Mol. Cell Biol. by NT Pfanner (2001) -
Martin, J. & Hartl, F. U. Chaperone-assisted protein folding. Curr. Opin. Struct. Biol. 7, 41–52 (1997).
(
10.1016/S0959-440X(97)80006-1
) / Curr. Opin. Struct. Biol. by J Martin (1997) -
Hartl, F. U. Molecular chaperones in cellular folding. Nature 381, 571–579 (1996).
(
10.1038/381571a0
) / Nature by FU Hartl (1996) -
Krimmer, T., Rassow, J., Kunau, W. H., Voos, W. & Pfanner, N. Mitochondrial protein import motor: the ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the peptide binding domain and the carboxy-terminal segment play a stimulatory role. Mol. Cell. Biol. 20, 5879–5887 (2000).
(
10.1128/MCB.20.16.5879-5887.2000
) / Mol. Cell. Biol. by T Krimmer (2000) -
Dekker, P. J. et al. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70–Tim44. EMBO J. 16, 5408–5419 (1997).
(
10.1093/emboj/16.17.5408
) / EMBO J. by PJ Dekker (1997) -
Spudich, J. A. How molecular motors work. Nature 372, 515–518 (1994).
(
10.1038/372515a0
) / Nature by JA Spudich (1994) -
Spudich, J. A. The myosin swinging cross-bridge model. Nature Rev. Mol. Cell Biol. 2, 387–392 (2001).
(
10.1038/35073086
) / Nature Rev. Mol. Cell Biol. by JA Spudich (2001) -
Eilers, M. & Schatz, G. Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature 322, 228–232 (1986).
(
10.1038/322228a0
) / Nature by M Eilers (1986) -
Rassow, J. et al. Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sites. J. Cell Biol. 109, 1421–1428 (1989).
(
10.1083/jcb.109.4.1421
) / J. Cell Biol. by J Rassow (1989) -
Gaume, B. et al. Unfolding of preproteins upon import into mitochondria. EMBO J. 17, 6497–6507 (1998).This study provides evidence that the kinetic stability of the amino terminus of a folded protein — rather than its overall thermodynamic stability — determines the rates of unfolding and import.
(
10.1093/emboj/17.22.6497
) / EMBO J. by B Gaume (1998) -
Rojo, E. E., Guiard, B., Neupert, W. & Stuart, R. A. Sorting of D–lactate dehydrogenase to the inner membrane of mitochondria. Analysis of topogenic signal and energetic requirements. J. Biol. Chem. 273, 8040–8047 (1998).
(
10.1074/jbc.273.14.8040
) / J. Biol. Chem. by EE Rojo (1998) -
Gärtner, F. et al. Mitochondrial import of subunit Va of cytochrome c oxidase characterized with yeast mutants. J. Biol. Chem. 270, 3788–3796 (1995).
(
10.1074/jbc.270.8.3788
) / J. Biol. Chem. by F Gärtner (1995) -
Strub, A., Rottgers, K. & Voos, W. The Hsp70 peptide-binding domain determines the interaction of the ATPase domain with Tim44 in mitochondria. EMBO J. 21, 2626–2635 (2002).
(
10.1093/emboj/21.11.2626
) / EMBO J. by A Strub (2002) -
Voisine, C. et al. The protein import motor of mitochondria: unfolding and trapping of preproteins are distinct and separable functions of matrix Hsp70. Cell 97, 565–574 (1999).This study shows that the interaction of mtHsp70 with Tim44 is essential for unfolding and import of preproteins. This observation has been used as an important argument in support of a pulling mechanism.
(
10.1016/S0092-8674(00)80768-0
) / Cell by C Voisine (1999) -
Laufen, T. et al. Mechanism of regulation of hsp70 chaperones by DnaJ cochaperones. Proc. Natl Acad. Sci. USA 96, 5452–5457 (1999).
(
10.1073/pnas.96.10.5452
) / Proc. Natl Acad. Sci. USA by T Laufen (1999) -
Merlin, A. et al. The J-related segment of Tim44 is essential for cell viability: a mutant Tim44 remains in the mitochondrial import site, but inefficiently recruits mtHsp70 and impairs protein translocation. J. Cell Biol. 145, 961–972 (1999).
(
10.1083/jcb.145.5.961
) / J. Cell Biol. by A Merlin (1999) -
Zhuang, X. et al. Fluorescence quenching: a tool for single-molecule protein-folding study. Proc. Natl Acad. Sci. USA 97, 14241–14244 (2000).This study shows that spontaneous local unfolding of tightly folded domains occurs in the millisecond range.
(
10.1073/pnas.97.26.14241
) / Proc. Natl Acad. Sci. USA by X Zhuang (2000) -
Huang, S., Ratliff, K. S., Schwartz, M. P., Spenner, J. M. & Matouschek, A. Mitochondria unfold precursor proteins by unravelling them from their N-termini. Nature Struct. Biol. 6, 1132–1138 (1999).This study provides evidence that the import machinery unfolds proteins from the amino terminus. The unfolding pathway on the surface of the mitochondria differs from unfolding in solution.
(
10.1038/70073
) / Nature Struct. Biol. by S Huang (1999) -
Lim, J. H., Martin, F., Guiard, B., Pfanner, N. & Voos, W. The mitochondrial Hsp70-dependent import system actively unfolds preproteins and shortens the lag phase of translocation. EMBO J. 20, 941–950 (2001).
(
10.1093/emboj/20.5.941
) / EMBO J. by JH Lim (2001) -
Vestweber, D. & Schatz, G. Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria. EMBO J. 7, 1147–1151 (1988).
(
10.1002/j.1460-2075.1988.tb02924.x
) / EMBO J. by D Vestweber (1988) -
Gambill, B. D. et al. A dual role for mitochondrial heat shock protein 70 in membrane translocation of preproteins. J. Cell Biol. 123, 109–117 (1993).
(
10.1083/jcb.123.1.109
) / J. Cell Biol. by BD Gambill (1993) -
Voos, W. et al. Differential requirement for the mitochondrial Hsp70–Tim44 complex in unfolding and translocation of preproteins. EMBO J. 15, 2668–2677 (1996).
(
10.1002/j.1460-2075.1996.tb00627.x
) / EMBO J. by W Voos (1996) -
Matouschek, A., Pfanner, N. & Voos, W. Protein unfolding by mitochondria. The Hsp70 import motor. EMBO Rep. 1, 404–410 (2000).
(
10.1093/embo-reports/kvd093
) / EMBO Rep. by A Matouschek (2000) -
Matouschek, A. et al. Active unfolding of precursor proteins during mitochondrial protein import. EMBO J. 16, 6727–6736 (1997).
(
10.1093/emboj/16.22.6727
) / EMBO J. by A Matouschek (1997) -
Carrion-Vazquez, M. et al. Mechanical and chemical unfolding of a single protein: a comparison. Proc. Natl Acad. Sci. USA 96, 3694–3699 (1999).This study used atomic-force microscopy measurements to show that the forces for mechanical unfolding of titin immunoglobulin-like domains are in the range of 200–280 pN.
(
10.1073/pnas.96.7.3694
) / Proc. Natl Acad. Sci. USA by M Carrion-Vazquez (1999) -
Marszalek, P. E. et al. Mechanical unfolding intermediates in titin modules. Nature 402, 100–103 (1999).
(
10.1038/47083
) / Nature by PE Marszalek (1999) -
Li, H., Oberhauser, A. F., Fowler, S. B., Clarke, J. & Fernandez, J. M. Atomic force microscopy reveals the mechanical design of a modular protein. Proc. Natl Acad. Sci. USA 97, 6527–6531 (2000).
(
10.1073/pnas.120048697
) / Proc. Natl Acad. Sci. USA by H Li (2000) -
Smith, D. A. & Radford, S. E. Protein folding: pulling back the frontiers. Curr. Biol. 10, R662–R664 (2000).
(
10.1016/S0960-9822(00)00681-3
) / Curr. Biol. by DA Smith (2000) -
Horst, M., Azem, A., Schatz, G. & Glick, B. S. What is the driving force for protein import into mitochondria? Biochim. Biophys. Acta 1318, 71–78 (1997).A detailed and careful discussion of the possible mechanism of the mitochondrial import motor, with a clear description of the power-stroke model.
(
10.1016/S0005-2728(96)00131-4
) / Biochim. Biophys. Acta by M Horst (1997) -
Misselwitz, B., Staeck, O. & Rapoport, T. A. J proteins catalytically activate Hsp70 molecules to trap a wide range of peptide sequences. Mol. Cell 2, 593–603 (1998).
(
10.1016/S1097-2765(00)80158-6
) / Mol. Cell by B Misselwitz (1998) -
Matlack, K. E. S., Misselwitz, B., Plath, K. & Rapoport, T. A. BiP acts as a molecular ratchet during posttranslational transport of prepro-α-factor across the ER membrane. Cell 97, 553–564 (1999).
(
10.1016/S0092-8674(00)80767-9
) / Cell by KES Matlack (1999)
Dates
Type | When |
---|---|
Created | 22 years, 11 months ago (Sept. 20, 2002, 4:25 p.m.) |
Deposited | 2 years, 3 months ago (May 19, 2023, 12:10 a.m.) |
Indexed | 49 minutes ago (Aug. 27, 2025, 12:09 a.m.) |
Issued | 23 years ago (Aug. 1, 2002) |
Published | 23 years ago (Aug. 1, 2002) |
Published Print | 23 years ago (Aug. 1, 2002) |
@article{Neupert_2002, title={The protein import motor of mitochondria}, volume={3}, ISSN={1471-0080}, url={http://dx.doi.org/10.1038/nrm878}, DOI={10.1038/nrm878}, number={8}, journal={Nature Reviews Molecular Cell Biology}, publisher={Springer Science and Business Media LLC}, author={Neupert, Walter and Brunner, Michael}, year={2002}, month=aug, pages={555–565} }