DOI: 10.1038/nprot.2007.291. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2

Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2

10.1038/nprot.2007.291

Crossref journal-article

Springer Science and Business Media LLC

Nature Protocols (297)

Bibliography

Chudakov, D. M., Lukyanov, S., & Lukyanov, K. A. (2007). Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. Nature Protocols, 2(8), 2024â2032.

Authors 3

Dmitriy M Chudakov (first)
Sergey Lukyanov (additional)
Konstantin A Lukyanov (additional)

References 26 Referenced 240

Lukyanov, K.A., Chudakov, D.M., Lukyanov, S. & Verkhusha, V.V. Innovation: photoactivatable fluorescent proteins. Nat. Rev. Mol. Cell Biol. 6, 885–891 (2005). (10.1038/nrm1741) / Nat. Rev. Mol. Cell Biol. by KA Lukyanov (2005)
Patterson, G.H. & Lippincott-Schwartz, J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873–1877 (2002). (10.1126/science.1074952) / Science by GH Patterson (2002)
Chudakov, D.M. et al. Photoswitchable cyan fluorescent protein for protein tracking. Nat. Biotechnol. 22, 1435–1439 (2004). (10.1038/nbt1025) / Nat. Biotechnol. by DM Chudakov (2004)
van Thor, J.J., Gensch, T., Hellingwerf, K.J. & Johnson, L.N. Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222. Nat. Struct. Biol. 9, 37–41 (2002). (10.1038/nsb739) / Nat. Struct. Biol. by JJ van Thor (2002)
Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H. & Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 12651–12656 (2002). (10.1073/pnas.202320599) / Proc. Natl. Acad. Sci. USA by R Ando (2002)
Wiedenmann, J. et al. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA 101, 15905–15910 (2004). (10.1073/pnas.0403668101) / Proc. Natl. Acad. Sci. USA by J Wiedenmann (2004)
Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N. & Miyawaki, A. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233–238 (2005). (10.1038/sj.embor.7400361) / EMBO Rep. by H Tsutsui (2005)
Gurskaya, N.G. et al. Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nat. Biotechnol. 24, 461–465 (2006). (10.1038/nbt1191) / Nat. Biotechnol. by NG Gurskaya (2006)
Mizuno, H. et al. Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein. Mol. Cell 12, 1051–1058 (2003). (10.1016/S1097-2765(03)00393-9) / Mol. Cell by H Mizuno (2003)
Lukyanov, K.A. et al. Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog. J. Biol. Chem. 275, 25879–25882 (2000). (10.1074/jbc.C000338200) / J. Biol. Chem. by KA Lukyanov (2000)
Chudakov, D.M., Feofanov, A.V., Mudrik, N.N., Lukyanov, S. & Lukyanov, K.A. Chromophore environment provides clue to “kindling fluorescent protein” riddle. J. Biol. Chem. 278, 7215–7219 (2003). (10.1074/jbc.M211988200) / J. Biol. Chem. by DM Chudakov (2003)
Chudakov, D.M. et al. Kindling fluorescent proteins for precise in vivo photolabeling. Nat. Biotechnol. 21, 191–194 (2003). (10.1038/nbt778) / Nat. Biotechnol. by DM Chudakov (2003)
Ando, R., Mizuno, H. & Miyawaki, A. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370–1373 (2004). (10.1126/science.1102506) / Science by R Ando (2004)
Stiel, A.C. et al. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem. J. 402, 35–42 (2007). (10.1042/BJ20061401) / Biochem. J. by AC Stiel (2007)
Henderson, J.N., Ai, H.W., Campbell, R.E. & Remington, S.J. Structural basis for reversible photobleaching of a green fluorescent protein homologue. Proc. Natl. Acad. Sci. USA 104, 6672–6677 (2007). (10.1073/pnas.0700059104) / Proc. Natl. Acad. Sci. USA by JN Henderson (2007)
Andresen, M. et al. Structure and mechanism of the reversible photoswitch of a fluorescent protein. Proc. Natl. Acad. Sci. USA 102, 13070–13074 (2005). (10.1073/pnas.0502772102) / Proc. Natl. Acad. Sci. USA by M Andresen (2005)
Andresen, M. et al. Structural basis for reversible photoswitching in Dronpa. Proc. Natl. Acad. Sci. USA Jul 23 (2007) [Epub ahead of print]. (10.1073/pnas.0700629104)
Zhang, L. et al. Method for real-time monitoring of protein degradation at the single cell level. Biotechniques 42, 446, 448, 450 (2007).
Hofmann, M., Eggeling, C., Jakobs, S. & Hell, S.W. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc. Natl. Acad. Sci. USA 102, 17565–17569 (2005). (10.1073/pnas.0506010102) / Proc. Natl. Acad. Sci. USA by M Hofmann (2005)
Schwentker, M.A. et al. Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching. Microsc. Res. Tech. 70, 269–280 (2007). (10.1002/jemt.20443) / Microsc. Res. Tech. by MA Schwentker (2007)
Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006). (10.1126/science.1127344) / Science by E Betzig (2006)
Chudakov, D.M., Lukyanov, S. & Lukyanov, K.A. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol. 23, 605–613 (2005). (10.1016/j.tibtech.2005.10.005) / Trends Biotechnol. by DM Chudakov (2005)
Xia, J., Kim, S.H., Macmillan, S. & Truant, R. Practical three color live cell imaging by widefield microscopy. Biol. Proced. Online 8, 63–68 (2006). (10.1251/bpo119) / Biol. Proced. Online by J Xia (2006)
Post, J.N., Lidke, K.A., Rieger, B. & Arndt-Jovin, D.J. One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryos. FEBS Lett. 579, 325–330 (2005). (10.1016/j.febslet.2004.11.092) / FEBS Lett. by JN Post (2005)
Stark, D.A. & Kulesa, P.M. An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model. Dev. Dyn. 236, 1583–1594 (2007). (10.1002/dvdy.21174) / Dev. Dyn. by DA Stark (2007)
PerkinElmer. BioTechniques Protocol Guide 41, 63 (2006). / BioTechniques Protocol Guide by PerkinElmer. (2006)

Dates

Type	When
Created	18 years ago (Aug. 16, 2007, 12:25 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 8:06 p.m.)
Indexed	2 days, 19 hours ago (Aug. 26, 2025, 2:28 a.m.)
Issued	18 years ago (Aug. 1, 2007)
Published	18 years ago (Aug. 1, 2007)
Published Online	18 years ago (Aug. 16, 2007)
Published Print	18 years ago (Aug. 1, 2007)

Funders 0

None

BibTeX

@article{Chudakov_2007, title={Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2}, volume={2}, ISSN={1750-2799}, url={http://dx.doi.org/10.1038/nprot.2007.291}, DOI={10.1038/nprot.2007.291}, number={8}, journal={Nature Protocols}, publisher={Springer Science and Business Media LLC}, author={Chudakov, Dmitriy M and Lukyanov, Sergey and Lukyanov, Konstantin A}, year={2007}, month=aug, pages={2024–2032} }

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  ],
  "URL": "http://dx.doi.org/10.1038/nprot.2007.291",
  "relation": {},
  "ISSN": [
    "1754-2189",
    "1750-2799"
  ],
  "subject": [],
  "container-title-short": "Nat Protoc",
  "published": {
    "date-parts": [
      [
        2007,
        8
      ]
    ]
  }
}