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Hilpert, K., Winkler, D. F., & Hancock, R. E. (2007). Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion. Nature Protocols, 2(6), 1333–1349.

Authors 3
  1. Kai Hilpert (first)
  2. Dirk FH Winkler (additional)
  3. Robert EW Hancock (additional)
References 36 Referenced 236
  1. Sewald, N. & Jakubke, H.-D. Peptides—Chemistry and Biology 1st edn. (Wiley-VCH, Weinheim, Germany, 2002). (10.1002/352760068X) / Peptides—Chemistry and Biology by N Sewald (2002)
  2. Paschke, M. Phage display systems and their applications. Appl. Microbiol. Biotechnol. 70, 2–11 (2006). (10.1007/s00253-005-0270-9) / Appl. Microbiol. Biotechnol. by M Paschke (2006)
  3. Westerlund-Wikstrom, B. Peptide display on bacterial flagella: principles and applications. Int. J. Med. Microbiol. 290, 223–230 (2000). (10.1016/S1438-4221(00)80119-8) / Int. J. Med. Microbiol. by B Westerlund-Wikstrom (2000)
  4. Yan, X. & Xu, Z. Ribosome-display technology: applications for directed evolution of functional proteins. Drug Discov. Today 11, 911–916 (2006). (10.1016/j.drudis.2006.08.012) / Drug Discov. Today by X Yan (2006)
  5. Merrifield, R.B. Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. J. Am. Chem. Soc. 85, 2149–2154 (1963). (10.1021/ja00897a025) / J. Am. Chem. Soc. by RB Merrifield (1963)
  6. McKay, F.C. & Albertson, N.F. New amine-masking groups for peptide synthesis. J. Am. Chem. Soc. 79, 4686–4690 (1957). (10.1021/ja01574a029) / J. Am. Chem. Soc. by FC McKay (1957)
  7. Carpino, L.A. Oxidative reaction of hydrazines. IV. Elimination of nitrogen from 1,1-disubstituted-2-arenesulfonhydrazides. J. Am. Chem. Soc. 79, 4427–4431 (1957). (10.1021/ja01573a050) / J. Am. Chem. Soc. by LA Carpino (1957)
  8. Anderson, G.W. & McGregor, A.C. t-Butyloxycarbonylamino acids and their use in peptide synthesis. J. Am. Chem. Soc. 79, 6180–6183 (1957). (10.1021/ja01580a020) / J. Am. Chem. Soc. by GW Anderson (1957)
  9. Carpino, L.A. & Han, G.Y. The 9-fluorenylmethoxycarbonyl amino-protecting group. J. Org. Chem. 37, 3404–3409 (1972). (10.1021/jo00795a005) / J. Org. Chem. by LA Carpino (1972)
  10. Houghten, R.A. General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen–antibody interaction at the level of individual amino acids. Proc. Natl. Acad. Sci. USA 82, 5131–5135 (1985). (10.1073/pnas.82.15.5131) / Proc. Natl. Acad. Sci. USA by RA Houghten (1985)
  11. Pellois, J.P. et al. Individually addressable parallel peptide synthesis on microchips. Nat. Biotechnol. 20, 922–926 (2002). (10.1038/nbt723) / Nat. Biotechnol. by JP Pellois (2002)
  12. Geysen, H.M., Meloen, R.H. & Barteling, S.J. Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc. Natl. Acad. Sci. USA 81, 3998–4002 (1984). (10.1073/pnas.81.13.3998) / Proc. Natl. Acad. Sci. USA by HM Geysen (1984)
  13. Frank, R. Spot synthesis: an easy technique for positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217–9232 (1992). (10.1016/S0040-4020(01)85612-X) / Tetrahedron by R Frank (1992)
  14. Kramer, A. & Schneider-Mergener, J. Synthesis and screening of peptide libraries on continuous cellulose membrane supports. Methods Mol. Biol. 87, 25–39 (1998). / Methods Mol. Biol. by A Kramer (1998)
  15. Toepert, F. et al. Combining SPOT synthesis and native peptide ligation to create large arrays of WW protein domains. Angew. Chem. Int. Ed. Engl. 42, 1136–1140 (2003). (10.1002/anie.200390298) / Angew. Chem. Int. Ed. Engl. by F Toepert (2003)
  16. Heine, N. et al. Synthesis and screening of peptoid arrays on cellulose membranes. Tetrahedron 59, 9919–9930 (2003). (10.1016/j.tet.2003.10.044) / Tetrahedron by N Heine (2003)
  17. Blackwell, H.E. Hitting the SPOT: small-molecule macroarrays advance combinatorial synthesis. Curr. Opin. Chem. Biol. 10, 203–212 (2006). (10.1016/j.cbpa.2006.04.026) / Curr. Opin. Chem. Biol. by HE Blackwell (2006)
  18. Hilpert, K., Hansen, G., Wessner, H., Volkmer-Engert, R. & Hohne, W. Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases. J. Biochem. (Tokyo) 138, 383–390 (2005). (10.1093/jb/mvi140) / J. Biochem. (Tokyo) by K Hilpert (2005)
  19. Kramer, A. et al. Molecular basis for the binding promiscuity of an anti-p24 (HIV-1) monoclonal antibody. Cell 91, 799–809 (1997). (10.1016/S0092-8674(00)80468-7) / Cell by A Kramer (1997)
  20. Kramer, A. et al. Spot synthesis: observations and optimizations. J. Pept. Res. 54, 319–327 (1999). (10.1034/j.1399-3011.1999.00108.x) / J. Pept. Res. by A Kramer (1999)
  21. Weiser, A.A. et al. SPOT synthesis: reliability of array-based measurement of peptide binding affinity. Anal. Biochem. 342, 300–311 (2005). (10.1016/j.ab.2005.04.033) / Anal. Biochem. by AA Weiser (2005)
  22. Molina, F., Laune, D., Gougat, C., Pau, B. & Granier, C. Improved performances of spot multiple peptide synthesis. Pept. Res. 9, 151–155 (1996). / Pept. Res. by F Molina (1996)
  23. Geysen, H.M., Barteling, S.J. & Meloen, R.H. Small peptides induce antibodies with a sequence and structural requirement for binding antigen comparable to antibodies raised against the native protein. Proc. Natl. Acad. Sci. USA 82, 178–182 (1985). (10.1073/pnas.82.1.178) / Proc. Natl. Acad. Sci. USA by HM Geysen (1985)
  24. Houghten, R.A. et al. Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery. Nature 354, 84–86 (1991). (10.1038/354084a0) / Nature by RA Houghten (1991)
  25. Zander, N., Beutling, U., Dikmans, A., Thiele, S. & Frank, R. A special cellulose membrane support for the combinatorial and parallel synthesis of peptide libraries suitable for the SC2-type manufacturing of high density multi-purpose chemical micro-arrays. in Peptides 2004: Proceedings of the Third International and 28th European Peptide Symposium (eds. Flegel, M. et al.) 405–406 (2005). / Peptides 2004: Proceedings of the Third International and 28th European Peptide Symposium by N Zander (2005)
  26. Hilpert, K., Hansen, G., Wessner, H., Schneider-Mergener, J. & Hohne, W. Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis. J. Biochem. (Tokyo) 128, 1051–1057 (2000). (10.1093/oxfordjournals.jbchem.a022833) / J. Biochem. (Tokyo) by K Hilpert (2000)
  27. Hilpert, K., Volkmer-Engert, R., Walter, T. & Hancock, R.E. High-throughput generation of small antibacterial peptides with improved activity. Nat. Biotechnol. 23, 1008–1012 (2005). (10.1038/nbt1113) / Nat. Biotechnol. by K Hilpert (2005)
  28. Hilpert, K. & Hancock, R.E.W. Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose by peptide array technology. Nat Protocols (in the press).
  29. Kamradt, T. & Volkmer-Engert, R. Cross-reactivity of T lymphocytes in infection and autoimmunity. Mol. Divers. 8, 271–280 (2004). (10.1023/B:MODI.0000036236.11774.1b) / Mol. Divers. by T Kamradt (2004)
  30. Reineke, U. et al. Mapping protein–protein contact sites using cellulose-bound peptide scans. Mol. Divers. 1, 141–148 (1996). (10.1007/BF01544952) / Mol. Divers. by U Reineke (1996)
  31. Hilpert, K., Winkler, D.F.H. & Hancock, R.E.W. Cellulose-bound peptide arrays: preparation and applications. Biotech. Genet. Eng. Rev. 24 (in the press). (10.1080/02648725.2007.10648093)
  32. Fields, G.B. & Noble, R.L. Solid phase synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Pept. Prot. Res. 35, 161–214 (1990). (10.1111/j.1399-3011.1990.tb00939.x) / Int. J. Pept. Prot. Res. by GB Fields (1990)
  33. Krchnak, V., Vagner, J. & Lebl, M. Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicator. Int. J. Pept. Protein Res. 32, 415–416 (1988). (10.1111/j.1399-3011.1988.tb01276.x) / Int. J. Pept. Protein Res. by V Krchnak (1988)
  34. Gausepohl, H. & Behn, C. Automated synthesis of solid-phase bound peptides. in Peptide Arrays on Membrane Support (eds. Koch, J. & Mahler, M.) 55–68 (Springer, Berlin, Heidelberg, 2002). (10.1007/978-3-662-09229-3_4) / Peptide Arrays on Membrane Support by H Gausepohl (2002)
  35. Bowman, M.D., Jacobson, M.M., Pujanauski, B.G. & Blackwell, H.E. Efficient synthesis of small molecule macroarrays: optimization of the macroarray synthesis platform and examination of microwave and conventional heating methods. Tetrahedron 62, 4715–4727 (2006). (10.1016/j.tet.2006.02.083) / Tetrahedron by MD Bowman (2006)
  36. Takahashi, M., Ueno, A. & Mihara, H. Peptide design based on an antibody complementarity-determining region (CDR): construction of porphyrin-binding peptides and their affinity maturation by a combinatorial method. Chem. Eur. J. 6, 3196–3203 (2000). (10.1002/1521-3765(20000901)6:17<3196::AID-CHEM3196>3.0.CO;2-T) / Chem. Eur. J. by M Takahashi (2000)
Dates
Type When
Created 18 years, 3 months ago (May 25, 2007, 3:22 a.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 8:05 p.m.)
Indexed 5 hours, 22 minutes ago (Aug. 30, 2025, 12:18 p.m.)
Issued 18 years, 3 months ago (May 24, 2007)
Published 18 years, 3 months ago (May 24, 2007)
Published Online 18 years, 3 months ago (May 24, 2007)
Published Print 18 years, 2 months ago (June 1, 2007)
Funders 0

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@article{Hilpert_2007, title={Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion}, volume={2}, ISSN={1750-2799}, url={http://dx.doi.org/10.1038/nprot.2007.160}, DOI={10.1038/nprot.2007.160}, number={6}, journal={Nature Protocols}, publisher={Springer Science and Business Media LLC}, author={Hilpert, Kai and Winkler, Dirk FH and Hancock, Robert EW}, year={2007}, month=may, pages={1333–1349} }