DOI: 10.1038/nprot.2006.129. Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes

Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes

10.1038/nprot.2006.129

Crossref journal-article

Springer Science and Business Media LLC

Nature Protocols (297)

Bibliography

Pratt, J. M., Simpson, D. M., Doherty, M. K., Rivers, J., Gaskell, S. J., & Beynon, R. J. (2006). Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes. Nature Protocols, 1(2), 1029â1043.

Authors 6

Julie M Pratt (first)
Deborah M Simpson (additional)
Mary K Doherty (additional)
Jenny Rivers (additional)
Simon J Gaskell (additional)
Robert J Beynon (additional)

References 19 Referenced 338

Julka, S. & Regnier, F.E. Recent advancements in differential proteomics based on stable isotope coding. Brief. Funct. Genomic. Proteomic. 4, 158–177 (2005). (10.1093/bfgp/4.2.158) / Brief. Funct. Genomic. Proteomic. by S Julka (2005)
Ong, S.E. & Mann, M. Mass spectrometry–based proteomics turns quantitative. Nat. Chem. Biol. 1, 252–262 (2005). (10.1038/nchembio736) / Nat. Chem. Biol. by SE Ong (2005)
Yan, W. & Chen, S.S. Mass spectrometry–based quantitative proteomic profiling. Brief. Funct. Genomic. Proteomic. 4, 27–38 (2005). (10.1093/bfgp/4.1.27) / Brief. Funct. Genomic. Proteomic. by W Yan (2005)
Gerber, S.A., Rush, J., Stemman, O., Kirschner, M.W. & Gygi, S.P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. USA 100, 6940–6945 (2003). (10.1073/pnas.0832254100) / Proc. Natl. Acad. Sci. USA by SA Gerber (2003)
Kirkpatrick, D.S., Gerber, S.A. & Gygi, S.P. The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications. Methods 35, 265–273 (2005). (10.1016/j.ymeth.2004.08.018) / Methods by DS Kirkpatrick (2005)
Beynon, R.J., Doherty, M.K., Pratt, J.M. & Gaskell, S.J. Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides. Nat. Methods 2, 587–589 (2005). (10.1038/nmeth774) / Nat. Methods by RJ Beynon (2005)
Wu, C.C. & Yates, J.R. The application of mass spectrometry to membrane proteomics. Nat. Biotechnol. 21, 262–267 (2003). (10.1038/nbt0303-262) / Nat. Biotechnol. by CC Wu (2003)
Lu, X. & Zhu, H. A novel proteomic approach for high throughput analysis of membrane proteins. Mol. Cell Proteomics 4, 1948–1958 (2005). (10.1074/mcp.M500138-MCP200) / Mol. Cell Proteomics by X Lu (2005)
Fischer, F. & Poetsch, A. Protein cleavage strategies for an improved analysis of the membrane proteome. Proteome Sci. 4, 1–12 (2006). (10.1186/1477-5956-4-2) / Proteome Sci. by F Fischer (2006)
Brancia, F.L. et al. A combination of chemical derivatisation and improved bioinformatic tools optimises protein identification for proteomics. Electrophoresis 22, 552–559 (2001). (10.1002/1522-2683(200102)22:3<552::AID-ELPS552>3.0.CO;2-C) / Electrophoresis by FL Brancia (2001)
Beardsley, R.L. & Reilly, J.P. Optimization of guanidination procedures for MALDI mass mapping. Anal. Chem. 74, 1884–1890 (2002). (10.1021/ac015613o) / Anal. Chem. by RL Beardsley (2002)
Thevis, M., Ogorzalek Loo, R.R. & Loo, J.A. In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry. J. Proteome Res. 2, 163–172 (2003). (10.1021/pr025568g) / J. Proteome Res. by M Thevis (2003)
Beynon, R.J. & Pratt, J.M. Metabolic labelling of proteins for proteomics. Mol. Cell Proteomics 4, 857–8872 (2005). (10.1074/mcp.R400010-MCP200) / Mol. Cell Proteomics by RJ Beynon (2005)
Ellison, D., Hinton, J., Hubbard, S.J. & Beynon, R.J. Limited proteolysis of native proteins: the interaction between avidin and proteinase K. Protein Sci. 4, 1337–1345 (1995). (10.1002/pro.5560040709) / Protein Sci. by D Ellison (1995)
Hubbard, S.J. The structural aspects of limited proteolysis of native proteins. Biochim. Biophys. Acta 1382, 191–206 (1998). (10.1016/S0167-4838(97)00175-1) / Biochim. Biophys. Acta by SJ Hubbard (1998)
Hubbard, S.J., Beynon, R.J. & Thornton, J.M. Assessment of conformational parameters as predictors of limited proteolytic sites in native protein structures. Protein Eng. 11, 349–359 (1998). (10.1093/protein/11.5.349) / Protein Eng. by SJ Hubbard (1998)
Wu, C., Robertson, D.H., Hubbard, S.J., Gaskell, S.J. & Beynon, R.J. Proteolysis of native proteins. Trapping of a reaction intermediate. J. Biol. Chem. 274, 1108–1115 (1999). (10.1074/jbc.274.2.1108) / J. Biol. Chem. by C Wu (1999)
Hubbard, S.J. & Beynon, R.J. Proteolysis of native proteins as a structural probe. In Proteolytic Enzymes. A Practical Approach (eds. Beynon, R.J. & Bond, J.S.) 233–264 (Oxford University Press, Oxford, 2001). / Proteolytic Enzymes. A Practical Approach by SJ Hubbard (2001)
Sambrook, J. & Russell, D.W. (eds.) Preparation and transformation of competent E. coli using calcium chloride (Protocol 25). In Molecular Cloning: A Laboratory Manual 3rd Edn. Vol 1. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2001).

Dates

Type	When
Created	19 years ago (Aug. 17, 2006, 1:21 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 8 p.m.)
Indexed	1 day, 4 hours ago (Sept. 3, 2025, 6:56 a.m.)
Issued	19 years, 1 month ago (Aug. 1, 2006)
Published	19 years, 1 month ago (Aug. 1, 2006)
Published Online	19 years ago (Aug. 17, 2006)
Published Print	19 years, 1 month ago (Aug. 1, 2006)

Funders 0

None

BibTeX

@article{Pratt_2006, title={Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes}, volume={1}, ISSN={1750-2799}, url={http://dx.doi.org/10.1038/nprot.2006.129}, DOI={10.1038/nprot.2006.129}, number={2}, journal={Nature Protocols}, publisher={Springer Science and Business Media LLC}, author={Pratt, Julie M and Simpson, Deborah M and Doherty, Mary K and Rivers, Jenny and Gaskell, Simon J and Beynon, Robert J}, year={2006}, month=aug, pages={1029–1043} }

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