Crossref
journal-article
Springer Science and Business Media LLC
Nature Methods (297)
References
36
Referenced
130
-
Elbashir, S.M. et al. Duplexes of 21–nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494–498 (2001).
(
10.1038/35078107
) / Nature by SM Elbashir (2001) -
Cullen, B.R. Transcription and processing of human microRNA precursors. Mol. Cell 16, 861–865 (2004).
(
10.1016/j.molcel.2004.12.002
) / Mol. Cell by BR Cullen (2004) -
Hammond, S.M., Bernstein, E., Beach, D. & Hannon, G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404, 293–295 (2000).
(
10.1038/35005107
) / Nature by SM Hammond (2000) -
Schwarz, D.S., Hutvágner, G., Haley, B. & Zamore, P.D. Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways. Mol. Cell 10, 537–548 (2002).
(
10.1016/S1097-2765(02)00651-2
) / Mol. Cell by DS Schwarz (2002) -
Khvorova, A., Reynolds, A. & Jayasena, S.D. Functional siRNAs and miRNAs exhibit strand bias. Cell 115, 209–216 (2003).
(
10.1016/S0092-8674(03)00801-8
) / Cell by A Khvorova (2003) -
Schwarz, D.S. et al. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115, 199–208 (2003).
(
10.1016/S0092-8674(03)00759-1
) / Cell by DS Schwarz (2003) -
Paddison, P.J., Caudy, A.A., Bernstein, E., Hannon, G.J. & Conklin, D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948–958 (2002).
(
10.1101/gad.981002
) / Genes Dev. by PJ Paddison (2002) -
Brummelkamp, T.R., Bernards, R. & Agami, R. A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550–553 (2002).
(
10.1126/science.1068999
) / Science by TR Brummelkamp (2002) -
Zeng, Y., Wagner, E.J. & Cullen, B.R. Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol. Cell 9, 1327–1333 (2002).
(
10.1016/S1097-2765(02)00541-5
) / Mol. Cell by Y Zeng (2002) -
Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J. & Elledge, S.J. A lentiviral micro-RNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102, 13212–13217 (2005).
(
10.1073/pnas.0506306102
) / Proc. Natl. Acad. Sci. USA by F Stegmeier (2005) -
Lee, Y. et al. The nuclear RNase III Drosha initiates microRNA processing. Nature 425, 415–419 (2003).
(
10.1038/nature01957
) / Nature by Y Lee (2003) -
Bernstein, E., Caudy, A.A., Hammond, S.M. & Hannon, G.J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363–366 (2001).
(
10.1038/35053110
) / Nature by E Bernstein (2001) -
Bridge, A.J., Pebernard, S., Ducraux, A., Nicoulaz, A-L. & Iggo, R. Induction of an interferon response by RNAi vectors in mammalian cells. Nat. Genet. 34, 263–264 (2003).
(
10.1038/ng1173
) / Nat. Genet. by AJ Bridge (2003) -
Fish, R.J. & Kruithof, E.K. Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. BMC Mol. Biol. 5, 9 (2004).
(
10.1186/1471-2199-5-9
) / BMC Mol. Biol. by RJ Fish (2004) -
Sledz, C.A., Holko, M., de Veer, M.J., Silverman, R.H. & Williams, B.R.G. Activation of the interferon system by short-interfering RNAs. Nat. Cell Biol. 5, 834–839 (2003).
(
10.1038/ncb1038
) / Nat. Cell Biol. by CA Sledz (2003) -
Judge, A.D. et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat. Biotechnol. 23, 457–462 (2005).
(
10.1038/nbt1081
) / Nat. Biotechnol. by AD Judge (2005) -
Hornung, V. et al. Sequence-specific potent induction of interferon-α by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat. Med. 11, 263–270 (2005).
(
10.1038/nm1191
) / Nat. Med. by V Hornung (2005) -
Jackson, A.L. et al. Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21, 635–637 (2003).
(
10.1038/nbt831
) / Nat. Biotechnol. by AL Jackson (2003) -
Jackson, A.L. et al. Widespread siRNA “off-target”transcript silencing mediated by seed region sequence complementarity. RNA 12, 1179–1187 (2006).
(
10.1261/rna.25706
) / RNA by AL Jackson (2006) -
Birmingham, A. et al. 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. Nat. Methods 3, 199–204 (2006).
(
10.1038/nmeth854
) / Nat. Methods by A Birmingham (2006) -
Manche, L., Green, S.R., Schmedt, C. & Mathews, M.B. Interactions between double-stranded RNA regulators and the protein kinase DAI. Mol. Cell. Biol. 12, 5238–5248 (1992).
(
10.1128/MCB.12.11.5238
) / Mol. Cell. Biol. by L Manche (1992) -
Persengiev, S.P., Zhu, X. & Green, M.R. Nonspecific concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). RNA 10, 12–18 (2004).
(
10.1261/rna5160904
) / RNA by SP Persengiev (2004) -
Semizarov, D. et al. Specificity of short interfering RNA determined through gene expression signatures. Proc. Natl. Acad. Sci. USA 100, 6347–6352 (2003).
(
10.1073/pnas.1131959100
) / Proc. Natl. Acad. Sci. USA by D Semizarov (2003) -
Reynolds, A. et al. Induction of the interferon response by siRNA is cell type- and duplex length-dependent. RNA 12, 988–993 (2006).
(
10.1261/rna.2340906
) / RNA by A Reynolds (2006) -
Yi, R., Doehle, B.P., Qin, Y., Macara, I.G. & Cullen, B.R. Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. RNA 11, 220–226 (2005).
(
10.1261/rna.7233305
) / RNA by R Yi (2005) -
Pei, Y. & Tuschl, T. On the art of identifying effective and specific siRNAs. Nat. Methods 3, 670–676 (2006).
(
10.1038/nmeth911
) / Nat. Methods by Y Pei (2006) -
Cullen, B.R. Induction of stable RNA interference in mammalian cells. Gene Ther. 13, 503–508 (2006).
(
10.1038/sj.gt.3302656
) / Gene Ther. by BR Cullen (2006) -
Lagos-Quintana, M., Rauhut, R., Lendeckel, W. & Tuschl, T. Identification of novel genes coding for small expressed RNAs. Science 294, 853–858 (2001).
(
10.1126/science.1064921
) / Science by M Lagos-Quintana (2001) -
Akusjarvi, G., Svensson, C. & Nygard, O. A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay. Mol. Cell. Biol. 7, 549–551 (1987).
(
10.1128/MCB.7.1.549
) / Mol. Cell. Biol. by G Akusjarvi (1987) -
Scacheri, P.C. et al. Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc. Natl. Acad. Sci. USA 101, 1892–1897 (2004).
(
10.1073/pnas.0308698100
) / Proc. Natl. Acad. Sci. USA by PC Scacheri (2004) -
Fedorov, Y. et al. Off-target effects by siRNA can induce toxic phenotype. RNA 12, 1188–1196 (2006).
(
10.1261/rna.28106
) / RNA by Y Fedorov (2006) -
Tiscornia, G., Singer, O., Ikawa, M. & Verma, I.M. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc. Natl. Acad. Sci. USA 100, 1844–1848 (2003).
(
10.1073/pnas.0437912100
) / Proc. Natl. Acad. Sci. USA by G Tiscornia (2003) -
Rubinson, D.A. et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33, 401–406 (2003).
(
10.1038/ng1117
) / Nat. Genet. by DA Rubinson (2003) -
Zeng, Y. & Cullen, B.R. RNA interference in human cells is restricted to the cytoplasm. RNA 8, 855–860 (2002).
(
10.1017/S1355838202020071
) / RNA by Y Zeng (2002) -
hither RNAi? Nat. Cell Biol. 5, 489–490 (2003).
(
10.1038/ncb0603-490
) -
Myers, J.W., Jones, J.T., Meyer, T. & Ferrell, J.E., Jr. Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing. Nat. Biotechnol. 21, 324–328 (2003).
(
10.1038/nbt792
) / Nat. Biotechnol. by JW Myers (2003)
Dates
Type | When |
---|---|
Created | 19 years ago (Aug. 23, 2006, 2:20 p.m.) |
Deposited | 2 years, 3 months ago (May 18, 2023, 8 p.m.) |
Indexed | 2 months, 3 weeks ago (June 9, 2025, 1:27 p.m.) |
Issued | 19 years ago (Aug. 23, 2006) |
Published | 19 years ago (Aug. 23, 2006) |
Published Online | 19 years ago (Aug. 23, 2006) |
Published Print | 19 years ago (Sept. 1, 2006) |
@article{Cullen_2006, title={Enhancing and confirming the specificity of RNAi experiments}, volume={3}, ISSN={1548-7105}, url={http://dx.doi.org/10.1038/nmeth913}, DOI={10.1038/nmeth913}, number={9}, journal={Nature Methods}, publisher={Springer Science and Business Media LLC}, author={Cullen, Bryan R}, year={2006}, month=aug, pages={677–681} }