DOI: 10.1038/nmeth.2488. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms

Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms

10.1038/nmeth.2488

Crossref journal-article

Springer Science and Business Media LLC

Nature Methods (297)

Bibliography

Huang, F., Hartwich, T. M. P., Rivera-Molina, F. E., Lin, Y., Duim, W. C., Long, J. J., Uchil, P. D., Myers, J. R., Baird, M. A., Mothes, W., Davidson, M. W., Toomre, D., & Bewersdorf, J. (2013). Video-rate nanoscopy using sCMOS cameraâspecific single-molecule localization algorithms. Nature Methods, 10(7), 653â658.

Authors 13

Fang Huang (first)
Tobias M P Hartwich (additional)
Felix E Rivera-Molina (additional)
Yu Lin (additional)
Whitney C Duim (additional)
Jane J Long (additional)
Pradeep D Uchil (additional)
Jordan R Myers (additional)
Michelle A Baird (additional)
Walther Mothes (additional)
Michael W Davidson (additional)
Derek Toomre (additional)
Joerg Bewersdorf (additional)

References 32 Referenced 480

Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24–32 (2009). (10.1038/nmeth.1291) / Nat. Methods by SW Hell (2009)
Gould, T.J., Hess, S.T. & Bewersdorf, J. Optical nanoscopy: from acquisition to analysis. Annu. Rev. Biomed. Eng. 14, 231–254 (2012). (10.1146/annurev-bioeng-071811-150025) / Annu. Rev. Biomed. Eng. by TJ Gould (2012)
van de Linde, S., Heilemann, M. & Sauer, M. Live-cell super-resolution imaging with synthetic fluorophores. Annu. Rev. Phys. Chem. 63, 519–540 (2012). (10.1146/annurev-physchem-032811-112012) / Annu. Rev. Phys. Chem. by S van de Linde (2012)
Mortensen, K.I., Churchman, L.S., Spudich, J.A. & Flyvbjerg, H. Optimized localization analysis for single-molecule tracking and super-resolution microscopy. Nat. Methods 7, 377–381 (2010). (10.1038/nmeth.1447) / Nat. Methods by KI Mortensen (2010)
Chao, J., Ram, S., Ward, E.S. & Ober, R.J. Ultrahigh accuracy imaging modality for super-localization microscopy. Nat. Methods 10, 335–338 (2013). (10.1038/nmeth.2396) / Nat. Methods by J Chao (2013)
Hess, S.T. et al. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proc. Natl. Acad. Sci. USA 104, 17370–17375 (2007). (10.1073/pnas.0708066104) / Proc. Natl. Acad. Sci. USA by ST Hess (2007)
Jones, S.A., Shim, S.-H., He, J. & Zhuang, X. Fast, three-dimensional super-resolution imaging of live cells. Nat. Methods 8, 499–505 (2011). (10.1038/nmeth.1605) / Nat. Methods by SA Jones (2011)
Shim, S.-H. et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc. Natl. Acad. Sci. USA 109, 13978–13983 (2012). (10.1073/pnas.1201882109) / Proc. Natl. Acad. Sci. USA by S-H Shim (2012)
Saurabh, S., Maji, S. & Bruchez, M.P. Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules. Opt. Express 20, 7338–7349 (2012). (10.1364/OE.20.007338) / Opt. Express by S Saurabh (2012)
Long, F., Zeng, S. & Huang, Z.-L. Localization-based super-resolution microscopy with an sCMOS camera. Part II: experimental methodology for comparing sCMOS with EMCCD cameras. Opt. Express 20, 17741–17759 (2012). (10.1364/OE.20.017741) / Opt. Express by F Long (2012)
Ober, R.J., Ram, S. & Ward, E.S. Localization accuracy in single-molecule microscopy. Biophys. J. 86, 1185–1200 (2004). (10.1016/S0006-3495(04)74193-4) / Biophys. J. by RJ Ober (2004)
Smith, C.S., Joseph, N., Rieger, B. & Lidke, K.A. Fast, single-molecule localization that achieves theoretically minimum uncertainty. Nat. Methods 7, 373–375 (2010). (10.1038/nmeth.1449) / Nat. Methods by CS Smith (2010)
Huang, F., Schwartz, S.L., Byars, J.M. & Lidke, K.A. Simultaneous multiple-emitter fitting for single molecule super-resolution imaging. Biomed. Opt. Express 2, 1377–1393 (2011). (10.1364/BOE.2.001377) / Biomed. Opt. Express by F Huang (2011)
Zhu, L., Zhang, W., Elnatan, D. & Huang, B. Faster STORM using compressed sensing. Nat. Methods 9, 721–723 (2012). (10.1038/nmeth.1978) / Nat. Methods by L Zhu (2012)
Zhang, M. et al. Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nat. Methods 9, 727–729 (2012). (10.1038/nmeth.2021) / Nat. Methods by M Zhang (2012)
Collins, A., Warrington, A., Taylor, K.A. & Svitkina, T. Structural organization of the actin cytoskeleton at sites of clathrin-mediated endocytosis. Curr. Biol. 21, 1167–1175 (2011). (10.1016/j.cub.2011.05.048) / Curr. Biol. by A Collins (2011)
Sironi, L. et al. Automatic quantification of microtubule dynamics enables RNAi-screening of new mitotic spindle regulators. Cytoskeleton (Hoboken) 68, 266–278 (2011). (10.1002/cm.20510) / Cytoskeleton (Hoboken) by L Sironi (2011)
Biteen, J.S., Goley, E.D., Shapiro, L. & Moerner, W.E. Three-dimensional super-resolution imaging of the midplane protein FtsZ in live Caulobacter crescentus cells using astigmatism. ChemPhysChem 13, 1007–1012 (2012). (10.1002/cphc.201100686) / ChemPhysChem by JS Biteen (2012)
Doherty, G.J. & McMahon, H.T. Mechanisms of endocytosis. Annu. Rev. Biochem. 78, 857–902 (2009). (10.1146/annurev.biochem.78.081307.110540) / Annu. Rev. Biochem. by GJ Doherty (2009)
Juette, M.F. & Bewersdorf, J. Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution. Nano Lett. 10, 4657–4663 (2010). (10.1021/nl1028792) / Nano Lett. by MF Juette (2010)
Lukinavičius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat. Chem. 5, 132–139 (2013). (10.1038/nchem.1546) / Nat. Chem. by G Lukinavičius (2013)
Benke, A. & Manley, S. Live-cell dSTORM of cellular DNA based on direct DNA labeling. ChemBioChem 13, 298–301 (2012). (10.1002/cbic.201100679) / ChemBioChem by A Benke (2012)
Vaughan, J.C., Jia, S. & Zhuang, X. Ultrabright photoactivatable fluorophores created by reductive caging. Nat. Methods 9, 1181–1184 (2012). (10.1038/nmeth.2214) / Nat. Methods by JC Vaughan (2012)
Lee, M.K., Williams, J., Twieg, R.J., Rao, J. & Moerner, W.E. Enzymatic activation of nitro-aryl fluorogens in live bacterial cells for enzymatic turnover-activated localization microscopy. Chem. Sci. 4, 220–225 (2013). (10.1039/C2SC21074F) / Chem. Sci. by MK Lee (2013)
Manley, S. et al. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nat. Methods 5, 155–157 (2008). (10.1038/nmeth.1176) / Nat. Methods by S Manley (2008)
Juette, M.F. et al. Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples. Nat. Methods 5, 527–529 (2008). (10.1038/nmeth.1211) / Nat. Methods by MF Juette (2008)
Aquino, D. et al. Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores. Nat. Methods 8, 353–359 (2011). (10.1038/nmeth.1583) / Nat. Methods by D Aquino (2011)
Testa, I. et al. Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys. J. 99, 2686–2694 (2010). (10.1016/j.bpj.2010.08.012) / Biophys. J. by I Testa (2010)
Gunewardene, M.S. et al. Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells. Biophys. J. 101, 1522–1528 (2011). (10.1016/j.bpj.2011.07.049) / Biophys. J. by MS Gunewardene (2011)
Wilks, S.S. The large-sample distribution of the likelihood ratio for testing composite hypotheses. Ann. Math. Stat. 9, 60–62 (1938). (10.1214/aoms/1177732360) / Ann. Math. Stat. by SS Wilks (1938)
Shore, E. & Small, A. Optimal acquisition scheme for subwavelength localization microscopy of bleachable fluorophores. A. Opt. Lett. 36, 289–291 (2011). (10.1364/OL.36.000289) / Opt. Lett. by E Shore (2011)
Lidke, K.A., Rieger, B., Lidke, D.S. & Jovin, T.M. The role of photon statistics in fluorescence anisotropy imaging. IEEE Trans. Image Process. 14, 1237–1245 (2005). (10.1109/TIP.2005.852458) / IEEE Trans. Image Process. by KA Lidke (2005)

Dates

Type	When
Created	12 years, 3 months ago (May 26, 2013, 4:01 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 7:01 p.m.)
Indexed	1 week ago (Aug. 29, 2025, 5:42 a.m.)
Issued	12 years, 3 months ago (May 26, 2013)
Published	12 years, 3 months ago (May 26, 2013)
Published Online	12 years, 3 months ago (May 26, 2013)
Published Print	12 years, 2 months ago (July 1, 2013)

Funders 0

None

BibTeX

@article{Huang_2013, title={Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms}, volume={10}, ISSN={1548-7105}, url={http://dx.doi.org/10.1038/nmeth.2488}, DOI={10.1038/nmeth.2488}, number={7}, journal={Nature Methods}, publisher={Springer Science and Business Media LLC}, author={Huang, Fang and Hartwich, Tobias M P and Rivera-Molina, Felix E and Lin, Yu and Duim, Whitney C and Long, Jane J and Uchil, Pradeep D and Myers, Jordan R and Baird, Michelle A and Mothes, Walther and Davidson, Michael W and Toomre, Derek and Bewersdorf, Joerg}, year={2013}, month=may, pages={653–658} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        29
      ]
    ],
    "date-time": "2025-08-29T09:42:00Z",
    "timestamp": 1756460520630
  },
  "reference-count": 32,
  "publisher": "Springer Science and Business Media LLC",
  "issue": "7",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2013,
            5,
            26
          ]
        ],
        "date-time": "2013-05-26T00:00:00Z",
        "timestamp": 1369526400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "http://www.springer.com/tdm"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2013,
        7
      ]
    ]
  },
  "DOI": "10.1038/nmeth.2488",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        5,
        26
      ]
    ],
    "date-time": "2013-05-26T20:01:46Z",
    "timestamp": 1369598506000
  },
  "page": "653-658",
  "source": "Crossref",
  "is-referenced-by-count": 480,
  "title": "Video-rate nanoscopy using sCMOS camera\u2013specific single-molecule localization algorithms",
  "prefix": "10.1038",
  "volume": "10",
  "author": [
    {
      "given": "Fang",
      "family": "Huang",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Tobias M P",
      "family": "Hartwich",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Felix E",
      "family": "Rivera-Molina",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yu",
      "family": "Lin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Whitney C",
      "family": "Duim",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jane J",
      "family": "Long",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Pradeep D",
      "family": "Uchil",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jordan R",
      "family": "Myers",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Michelle A",
      "family": "Baird",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Walther",
      "family": "Mothes",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Michael W",
      "family": "Davidson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Derek",
      "family": "Toomre",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Joerg",
      "family": "Bewersdorf",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "297",
  "published-online": {
    "date-parts": [
      [
        2013,
        5,
        26
      ]
    ]
  },
  "reference": [
    {
      "key": "BFnmeth2488_CR1",
      "doi-asserted-by": "publisher",
      "first-page": "24",
      "DOI": "10.1038/nmeth.1291",
      "volume": "6",
      "author": "SW Hell",
      "year": "2009",
      "unstructured": "Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24\u201332 (2009).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR2",
      "doi-asserted-by": "publisher",
      "first-page": "231",
      "DOI": "10.1146/annurev-bioeng-071811-150025",
      "volume": "14",
      "author": "TJ Gould",
      "year": "2012",
      "unstructured": "Gould, T.J., Hess, S.T. & Bewersdorf, J. Optical nanoscopy: from acquisition to analysis. Annu. Rev. Biomed. Eng. 14, 231\u2013254 (2012).",
      "journal-title": "Annu. Rev. Biomed. Eng."
    },
    {
      "key": "BFnmeth2488_CR3",
      "doi-asserted-by": "publisher",
      "first-page": "519",
      "DOI": "10.1146/annurev-physchem-032811-112012",
      "volume": "63",
      "author": "S van de Linde",
      "year": "2012",
      "unstructured": "van de Linde, S., Heilemann, M. & Sauer, M. Live-cell super-resolution imaging with synthetic fluorophores. Annu. Rev. Phys. Chem. 63, 519\u2013540 (2012).",
      "journal-title": "Annu. Rev. Phys. Chem."
    },
    {
      "key": "BFnmeth2488_CR4",
      "doi-asserted-by": "publisher",
      "first-page": "377",
      "DOI": "10.1038/nmeth.1447",
      "volume": "7",
      "author": "KI Mortensen",
      "year": "2010",
      "unstructured": "Mortensen, K.I., Churchman, L.S., Spudich, J.A. & Flyvbjerg, H. Optimized localization analysis for single-molecule tracking and super-resolution microscopy. Nat. Methods 7, 377\u2013381 (2010).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR5",
      "doi-asserted-by": "publisher",
      "first-page": "335",
      "DOI": "10.1038/nmeth.2396",
      "volume": "10",
      "author": "J Chao",
      "year": "2013",
      "unstructured": "Chao, J., Ram, S., Ward, E.S. & Ober, R.J. Ultrahigh accuracy imaging modality for super-localization microscopy. Nat. Methods 10, 335\u2013338 (2013).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR6",
      "doi-asserted-by": "publisher",
      "first-page": "17370",
      "DOI": "10.1073/pnas.0708066104",
      "volume": "104",
      "author": "ST Hess",
      "year": "2007",
      "unstructured": "Hess, S.T. et al. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proc. Natl. Acad. Sci. USA 104, 17370\u201317375 (2007).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnmeth2488_CR7",
      "doi-asserted-by": "publisher",
      "first-page": "499",
      "DOI": "10.1038/nmeth.1605",
      "volume": "8",
      "author": "SA Jones",
      "year": "2011",
      "unstructured": "Jones, S.A., Shim, S.-H., He, J. & Zhuang, X. Fast, three-dimensional super-resolution imaging of live cells. Nat. Methods 8, 499\u2013505 (2011).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR8",
      "doi-asserted-by": "publisher",
      "first-page": "13978",
      "DOI": "10.1073/pnas.1201882109",
      "volume": "109",
      "author": "S-H Shim",
      "year": "2012",
      "unstructured": "Shim, S.-H. et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc. Natl. Acad. Sci. USA 109, 13978\u201313983 (2012).",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "BFnmeth2488_CR9",
      "doi-asserted-by": "publisher",
      "first-page": "7338",
      "DOI": "10.1364/OE.20.007338",
      "volume": "20",
      "author": "S Saurabh",
      "year": "2012",
      "unstructured": "Saurabh, S., Maji, S. & Bruchez, M.P. Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules. Opt. Express 20, 7338\u20137349 (2012).",
      "journal-title": "Opt. Express"
    },
    {
      "key": "BFnmeth2488_CR10",
      "doi-asserted-by": "publisher",
      "first-page": "17741",
      "DOI": "10.1364/OE.20.017741",
      "volume": "20",
      "author": "F Long",
      "year": "2012",
      "unstructured": "Long, F., Zeng, S. & Huang, Z.-L. Localization-based super-resolution microscopy with an sCMOS camera. Part II: experimental methodology for comparing sCMOS with EMCCD cameras. Opt. Express 20, 17741\u201317759 (2012).",
      "journal-title": "Opt. Express"
    },
    {
      "key": "BFnmeth2488_CR11",
      "doi-asserted-by": "publisher",
      "first-page": "1185",
      "DOI": "10.1016/S0006-3495(04)74193-4",
      "volume": "86",
      "author": "RJ Ober",
      "year": "2004",
      "unstructured": "Ober, R.J., Ram, S. & Ward, E.S. Localization accuracy in single-molecule microscopy. Biophys. J. 86, 1185\u20131200 (2004).",
      "journal-title": "Biophys. J."
    },
    {
      "key": "BFnmeth2488_CR12",
      "doi-asserted-by": "publisher",
      "first-page": "373",
      "DOI": "10.1038/nmeth.1449",
      "volume": "7",
      "author": "CS Smith",
      "year": "2010",
      "unstructured": "Smith, C.S., Joseph, N., Rieger, B. & Lidke, K.A. Fast, single-molecule localization that achieves theoretically minimum uncertainty. Nat. Methods 7, 373\u2013375 (2010).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR13",
      "doi-asserted-by": "publisher",
      "first-page": "1377",
      "DOI": "10.1364/BOE.2.001377",
      "volume": "2",
      "author": "F Huang",
      "year": "2011",
      "unstructured": "Huang, F., Schwartz, S.L., Byars, J.M. & Lidke, K.A. Simultaneous multiple-emitter fitting for single molecule super-resolution imaging. Biomed. Opt. Express 2, 1377\u20131393 (2011).",
      "journal-title": "Biomed. Opt. Express"
    },
    {
      "key": "BFnmeth2488_CR14",
      "doi-asserted-by": "publisher",
      "first-page": "721",
      "DOI": "10.1038/nmeth.1978",
      "volume": "9",
      "author": "L Zhu",
      "year": "2012",
      "unstructured": "Zhu, L., Zhang, W., Elnatan, D. & Huang, B. Faster STORM using compressed sensing. Nat. Methods 9, 721\u2013723 (2012).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR15",
      "doi-asserted-by": "publisher",
      "first-page": "727",
      "DOI": "10.1038/nmeth.2021",
      "volume": "9",
      "author": "M Zhang",
      "year": "2012",
      "unstructured": "Zhang, M. et al. Rational design of true monomeric and bright photoactivatable fluorescent proteins. Nat. Methods 9, 727\u2013729 (2012).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR16",
      "doi-asserted-by": "publisher",
      "first-page": "1167",
      "DOI": "10.1016/j.cub.2011.05.048",
      "volume": "21",
      "author": "A Collins",
      "year": "2011",
      "unstructured": "Collins, A., Warrington, A., Taylor, K.A. & Svitkina, T. Structural organization of the actin cytoskeleton at sites of clathrin-mediated endocytosis. Curr. Biol. 21, 1167\u20131175 (2011).",
      "journal-title": "Curr. Biol."
    },
    {
      "key": "BFnmeth2488_CR17",
      "doi-asserted-by": "publisher",
      "first-page": "266",
      "DOI": "10.1002/cm.20510",
      "volume": "68",
      "author": "L Sironi",
      "year": "2011",
      "unstructured": "Sironi, L. et al. Automatic quantification of microtubule dynamics enables RNAi-screening of new mitotic spindle regulators. Cytoskeleton (Hoboken) 68, 266\u2013278 (2011).",
      "journal-title": "Cytoskeleton (Hoboken)"
    },
    {
      "key": "BFnmeth2488_CR18",
      "doi-asserted-by": "publisher",
      "first-page": "1007",
      "DOI": "10.1002/cphc.201100686",
      "volume": "13",
      "author": "JS Biteen",
      "year": "2012",
      "unstructured": "Biteen, J.S., Goley, E.D., Shapiro, L. & Moerner, W.E. Three-dimensional super-resolution imaging of the midplane protein FtsZ in live Caulobacter crescentus cells using astigmatism. ChemPhysChem 13, 1007\u20131012 (2012).",
      "journal-title": "ChemPhysChem"
    },
    {
      "key": "BFnmeth2488_CR19",
      "doi-asserted-by": "publisher",
      "first-page": "857",
      "DOI": "10.1146/annurev.biochem.78.081307.110540",
      "volume": "78",
      "author": "GJ Doherty",
      "year": "2009",
      "unstructured": "Doherty, G.J. & McMahon, H.T. Mechanisms of endocytosis. Annu. Rev. Biochem. 78, 857\u2013902 (2009).",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "BFnmeth2488_CR20",
      "doi-asserted-by": "publisher",
      "first-page": "4657",
      "DOI": "10.1021/nl1028792",
      "volume": "10",
      "author": "MF Juette",
      "year": "2010",
      "unstructured": "Juette, M.F. & Bewersdorf, J. Three-dimensional tracking of single fluorescent particles with submillisecond temporal resolution. Nano Lett. 10, 4657\u20134663 (2010).",
      "journal-title": "Nano Lett."
    },
    {
      "key": "BFnmeth2488_CR21",
      "doi-asserted-by": "publisher",
      "first-page": "132",
      "DOI": "10.1038/nchem.1546",
      "volume": "5",
      "author": "G Lukinavi\u010dius",
      "year": "2013",
      "unstructured": "Lukinavi\u010dius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat. Chem. 5, 132\u2013139 (2013).",
      "journal-title": "Nat. Chem."
    },
    {
      "key": "BFnmeth2488_CR22",
      "doi-asserted-by": "publisher",
      "first-page": "298",
      "DOI": "10.1002/cbic.201100679",
      "volume": "13",
      "author": "A Benke",
      "year": "2012",
      "unstructured": "Benke, A. & Manley, S. Live-cell dSTORM of cellular DNA based on direct DNA labeling. ChemBioChem 13, 298\u2013301 (2012).",
      "journal-title": "ChemBioChem"
    },
    {
      "key": "BFnmeth2488_CR23",
      "doi-asserted-by": "publisher",
      "first-page": "1181",
      "DOI": "10.1038/nmeth.2214",
      "volume": "9",
      "author": "JC Vaughan",
      "year": "2012",
      "unstructured": "Vaughan, J.C., Jia, S. & Zhuang, X. Ultrabright photoactivatable fluorophores created by reductive caging. Nat. Methods 9, 1181\u20131184 (2012).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR24",
      "doi-asserted-by": "publisher",
      "first-page": "220",
      "DOI": "10.1039/C2SC21074F",
      "volume": "4",
      "author": "MK Lee",
      "year": "2013",
      "unstructured": "Lee, M.K., Williams, J., Twieg, R.J., Rao, J. & Moerner, W.E. Enzymatic activation of nitro-aryl fluorogens in live bacterial cells for enzymatic turnover-activated localization microscopy. Chem. Sci. 4, 220\u2013225 (2013).",
      "journal-title": "Chem. Sci."
    },
    {
      "key": "BFnmeth2488_CR25",
      "doi-asserted-by": "publisher",
      "first-page": "155",
      "DOI": "10.1038/nmeth.1176",
      "volume": "5",
      "author": "S Manley",
      "year": "2008",
      "unstructured": "Manley, S. et al. High-density mapping of single-molecule trajectories with photoactivated localization microscopy. Nat. Methods 5, 155\u2013157 (2008).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR26",
      "doi-asserted-by": "publisher",
      "first-page": "527",
      "DOI": "10.1038/nmeth.1211",
      "volume": "5",
      "author": "MF Juette",
      "year": "2008",
      "unstructured": "Juette, M.F. et al. Three-dimensional sub\u2013100 nm resolution fluorescence microscopy of thick samples. Nat. Methods 5, 527\u2013529 (2008).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR27",
      "doi-asserted-by": "publisher",
      "first-page": "353",
      "DOI": "10.1038/nmeth.1583",
      "volume": "8",
      "author": "D Aquino",
      "year": "2011",
      "unstructured": "Aquino, D. et al. Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores. Nat. Methods 8, 353\u2013359 (2011).",
      "journal-title": "Nat. Methods"
    },
    {
      "key": "BFnmeth2488_CR28",
      "doi-asserted-by": "publisher",
      "first-page": "2686",
      "DOI": "10.1016/j.bpj.2010.08.012",
      "volume": "99",
      "author": "I Testa",
      "year": "2010",
      "unstructured": "Testa, I. et al. Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys. J. 99, 2686\u20132694 (2010).",
      "journal-title": "Biophys. J."
    },
    {
      "key": "BFnmeth2488_CR29",
      "doi-asserted-by": "publisher",
      "first-page": "1522",
      "DOI": "10.1016/j.bpj.2011.07.049",
      "volume": "101",
      "author": "MS Gunewardene",
      "year": "2011",
      "unstructured": "Gunewardene, M.S. et al. Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells. Biophys. J. 101, 1522\u20131528 (2011).",
      "journal-title": "Biophys. J."
    },
    {
      "key": "BFnmeth2488_CR30",
      "doi-asserted-by": "publisher",
      "first-page": "60",
      "DOI": "10.1214/aoms/1177732360",
      "volume": "9",
      "author": "SS Wilks",
      "year": "1938",
      "unstructured": "Wilks, S.S. The large-sample distribution of the likelihood ratio for testing composite hypotheses. Ann. Math. Stat. 9, 60\u201362 (1938).",
      "journal-title": "Ann. Math. Stat."
    },
    {
      "key": "BFnmeth2488_CR31",
      "doi-asserted-by": "publisher",
      "first-page": "289",
      "DOI": "10.1364/OL.36.000289",
      "volume": "36",
      "author": "E Shore",
      "year": "2011",
      "unstructured": "Shore, E. & Small, A. Optimal acquisition scheme for subwavelength localization microscopy of bleachable fluorophores. A. Opt. Lett. 36, 289\u2013291 (2011).",
      "journal-title": "Opt. Lett."
    },
    {
      "key": "BFnmeth2488_CR32",
      "doi-asserted-by": "publisher",
      "first-page": "1237",
      "DOI": "10.1109/TIP.2005.852458",
      "volume": "14",
      "author": "KA Lidke",
      "year": "2005",
      "unstructured": "Lidke, K.A., Rieger, B., Lidke, D.S. & Jovin, T.M. The role of photon statistics in fluorescence anisotropy imaging. IEEE Trans. Image Process. 14, 1237\u20131245 (2005).",
      "journal-title": "IEEE Trans. Image Process."
    }
  ],
  "container-title": "Nature Methods",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://www.nature.com/articles/nmeth.2488.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://www.nature.com/articles/nmeth.2488",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "http://www.nature.com/articles/nmeth.2488.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        5,
        18
      ]
    ],
    "date-time": "2023-05-18T23:01:35Z",
    "timestamp": 1684450895000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.nature.com/articles/nmeth.2488"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2013,
        5,
        26
      ]
    ]
  },
  "references-count": 32,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          2013,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "BFnmeth2488"
  ],
  "URL": "http://dx.doi.org/10.1038/nmeth.2488",
  "relation": {},
  "ISSN": [
    "1548-7091",
    "1548-7105"
  ],
  "subject": [],
  "container-title-short": "Nat Methods",
  "published": {
    "date-parts": [
      [
        2013,
        5,
        26
      ]
    ]
  }
}