DOI: 10.1038/nmeth.1539. Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

10.1038/nmeth.1539

Crossref journal-article

Springer Science and Business Media LLC

Nature Methods (297)

Bibliography

Doyon, Y., Vo, T. D., Mendel, M. C., Greenberg, S. G., Wang, J., Xia, D. F., Miller, J. C., Urnov, F. D., Gregory, P. D., & Holmes, M. C. (2010). Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nature Methods, 8(1), 74â79.

Authors 10

Yannick Doyon (first)
Thuy D Vo (additional)
Matthew C Mendel (additional)
Shon G Greenberg (additional)
Jianbin Wang (additional)
Danny F Xia (additional)
Jeffrey C Miller (additional)
Fyodor D Urnov (additional)
Philip D Gregory (additional)
Michael C Holmes (additional)

References 25 Referenced 346

Kim, Y.G., Cha, J. & Chandrasegaran, S. Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc. Natl. Acad. Sci. USA 93, 1156–1160 (1996). (10.1073/pnas.93.3.1156) / Proc. Natl. Acad. Sci. USA by YG Kim (1996)
Klug, A. The discovery of zinc fingers and their applications in gene regulation and genome manipulation. Annu. Rev. Biochem. 79, 213–231 (2010). (10.1146/annurev-biochem-010909-095056) / Annu. Rev. Biochem. by A Klug (2010)
Carroll, D. Progress and prospects: zinc-finger nucleases as gene therapy agents. Gene Ther. 15, 1463–1468 (2008). (10.1038/gt.2008.145) / Gene Ther. by D Carroll (2008)
Urnov, F.D., Rebar, E.J., Holmes, M.C., Zhang, H.S. & Gregory, P.D. Genome editing with engineered zinc finger nucleases. Nat. Rev. Genet. 11, 636–646 (2010). (10.1038/nrg2842) / Nat. Rev. Genet. by FD Urnov (2010)
Kim, J.S., Lee, H.J. & Carroll, D. Genome editing with modularly assembled zinc-finger nucleases. Nat. Methods 7, 91 (author reply, 91–92) (2010). (10.1038/nmeth0210-91a) / Nat. Methods by JS Kim (2010)
Miller, J.C. et al. An improved zinc-finger nuclease architecture for highly specific genome editing. Nat. Biotechnol. 25, 778–785 (2007). (10.1038/nbt1319) / Nat. Biotechnol. by JC Miller (2007)
Szczepek, M. et al. Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat. Biotechnol. 25, 786–793 (2007). (10.1038/nbt1317) / Nat. Biotechnol. by M Szczepek (2007)
Handel, E.M., Alwin, S. & Cathomen, T. Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol. Ther. 17, 104–111 (2009). (10.1038/mt.2008.233) / Mol. Ther. by EM Handel (2009)
Urnov, F.D. et al. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 435, 646–651 (2005). (10.1038/nature03556) / Nature by FD Urnov (2005)
Sollu, C. et al. Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res. advance online publication 16 August 2010 (doi:10.1093/nar/gkq720). (10.1093/nar/gkq720) / Nucleic Acids Research by Cem Şöllü (2010)
Bitinaite, J., Wah, D.A., Aggarwal, A.K. & Schildkraut, I. FokI dimerization is required for DNA cleavage. Proc. Natl. Acad. Sci. USA 95, 10570–10575 (1998). (10.1073/pnas.95.18.10570) / Proc. Natl. Acad. Sci. USA by J Bitinaite (1998)
Cai, C.Q. et al. Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol. Biol. 69, 699–709 (2009). (10.1007/s11103-008-9449-7) / Plant Mol. Biol. by CQ Cai (2009)
Doyon, Y. et al. Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat. Biotechnol. 26, 702–708 (2008). (10.1038/nbt1409) / Nat. Biotechnol. by Y Doyon (2008)
Geurts, A.M. et al. Knockout rats via embryo microinjection of zinc-finger nucleases. Science 325, 433 (2009). (10.1126/science.1172447) / Science by AM Geurts (2009)
Shukla, V.K. et al. Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature 459, 437–441 (2009). (10.1038/nature07992) / Nature by VK Shukla (2009)
Hampsey, M. A review of phenotypes in Saccharomyces cerevisiae. Yeast 13, 1099–1133 (1997). (10.1002/(SICI)1097-0061(19970930)13:12<1099::AID-YEA177>3.0.CO;2-7) / Yeast by M Hampsey (1997)
Perez, E.E. et al. Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases. Nat. Biotechnol. 26, 808–816 (2008). (10.1038/nbt1410) / Nat. Biotechnol. by EE Perez (2008)
Muhlrad, D., Hunter, R. & Parker, R. A rapid method for localized mutagenesis of yeast genes. Yeast 8, 79–82 (1992). (10.1002/yea.320080202) / Yeast by D Muhlrad (1992)
Wah, D.A., Bitinaite, J., Schildkraut, I. & Aggarwal, A.K. Structure of FokI has implications for DNA cleavage. Proc. Natl. Acad. Sci. USA 95, 10564–10569 (1998). (10.1073/pnas.95.18.10564) / Proc. Natl. Acad. Sci. USA by DA Wah (1998)
Kumar, S. & Nussinov, R. Close-range electrostatic interactions in proteins. ChemBioChem 3, 604–617 (2002). (10.1002/1439-7633(20020703)3:7<604::AID-CBIC604>3.0.CO;2-X) / ChemBioChem by S Kumar (2002)
Guschin, D.Y. et al. A rapid and general assay for monitoring endogenous gene modification. Methods Mol. Biol. 649, 247–256 (2010). (10.1007/978-1-60761-753-2_15) / Methods Mol. Biol. by DY Guschin (2010)
Doyon, Y. et al. Transient cold shock enhances zinc-finger nuclease-mediated gene disruption. Nat. Methods 7, 459–460 (2010). (10.1038/nmeth.1456) / Nat. Methods by Y Doyon (2010)
Holt, N. et al. Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo. Nat. Biotechnol. 28, 839–847 (2010). (10.1038/nbt.1663) / Nat. Biotechnol. by N Holt (2010)
Del Prete, G.Q. et al. Derivation and characterization of a simian immunodeficiency virus SIVmac239 variant with tropism for CXCR4. J. Virol. 83, 9911–9922 (2009). (10.1128/JVI.00533-09) / J. Virol. by GQ Del Prete (2009)
Guo, J., Gaj, T. & Barbas, C.F., III. Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases. J. Mol. Biol. 400, 96–107 (2010). (10.1016/j.jmb.2010.04.060) / J. Mol. Biol. by J Guo (2010)

Dates

Type	When
Created	14 years, 9 months ago (Dec. 5, 2010, 2:28 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 6:43 p.m.)
Indexed	6 days, 4 hours ago (Aug. 30, 2025, 12:59 p.m.)
Issued	14 years, 9 months ago (Dec. 5, 2010)
Published	14 years, 9 months ago (Dec. 5, 2010)
Published Online	14 years, 9 months ago (Dec. 5, 2010)
Published Print	14 years, 8 months ago (Jan. 1, 2011)

Funders 0

None

BibTeX

@article{Doyon_2010, title={Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures}, volume={8}, ISSN={1548-7105}, url={http://dx.doi.org/10.1038/nmeth.1539}, DOI={10.1038/nmeth.1539}, number={1}, journal={Nature Methods}, publisher={Springer Science and Business Media LLC}, author={Doyon, Yannick and Vo, Thuy D and Mendel, Matthew C and Greenberg, Shon G and Wang, Jianbin and Xia, Danny F and Miller, Jeffrey C and Urnov, Fyodor D and Gregory, Philip D and Holmes, Michael C}, year={2010}, month=dec, pages={74–79} }

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    "date-time": "2023-05-18T22:43:12Z",
    "timestamp": 1684449792000
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  "resource": {
    "primary": {
      "URL": "https://www.nature.com/articles/nmeth.1539"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
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  "subject": [],
  "container-title-short": "Nat Methods",
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}