DOI: 10.1038/ncomms14516. Dissecting the molecular organization of the translocon-associated protein complex

Dissecting the molecular organization of the translocon-associated protein complex

10.1038/ncomms14516

Crossref journal-article

Springer Science and Business Media LLC

Nature Communications (297)

Abstract

AbstractIn eukaryotic cells, one-third of all proteins must be transported across or inserted into the endoplasmic reticulum (ER) membrane by the ER protein translocon. The translocon-associated protein (TRAP) complex is an integral component of the translocon, assisting the Sec61 protein-conducting channel by regulating signal sequence and transmembrane helix insertion in a substrate-dependent manner. Here we use cryo-electron tomography (CET) to study the structure of the native translocon in evolutionarily divergent organisms and disease-linked TRAP mutant fibroblasts from human patients. The structural differences detected by subtomogram analysis form a basis for dissecting the molecular organization of the TRAP complex. We assign positions to the four TRAP subunits within the complex, providing insights into their individual functions. The revealed molecular architecture of a central translocon component advances our understanding of membrane protein biogenesis and sheds light on the role of TRAP in human congenital disorders of glycosylation.

Bibliography

Pfeffer, S., Dudek, J., Schaffer, M., Ng, B. G., Albert, S., Plitzko, J. M., Baumeister, W., Zimmermann, R., Freeze, H. H., Engel, B. D., & FÃ¶rster, F. (2017). Dissecting the molecular organization of the translocon-associated protein complex. Nature Communications, 8(1).

Authors 11

Stefan Pfeffer (first)
Johanna Dudek (additional)
Miroslava Schaffer (additional)
Bobby G. Ng (additional)
Sahradha Albert (additional)
Jürgen M. Plitzko (additional)
Wolfgang Baumeister (additional)
Richard Zimmermann (additional)
Hudson H. Freeze (additional)
Benjamin D. Engel (additional)
Friedrich Förster (additional)

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Dates

Type	When
Created	8 years, 6 months ago (Feb. 20, 2017, 5:30 a.m.)
Deposited	2 years, 8 months ago (Dec. 22, 2022, 6:52 p.m.)
Indexed	1 month ago (July 19, 2025, 11:43 p.m.)
Issued	8 years, 6 months ago (Feb. 20, 2017)
Published	8 years, 6 months ago (Feb. 20, 2017)
Published Online	8 years, 6 months ago (Feb. 20, 2017)

Funders 0

None

BibTeX

@article{Pfeffer_2017, title={Dissecting the molecular organization of the translocon-associated protein complex}, volume={8}, ISSN={2041-1723}, url={http://dx.doi.org/10.1038/ncomms14516}, DOI={10.1038/ncomms14516}, number={1}, journal={Nature Communications}, publisher={Springer Science and Business Media LLC}, author={Pfeffer, Stefan and Dudek, Johanna and Schaffer, Miroslava and Ng, Bobby G. and Albert, Sahradha and Plitzko, Jürgen M. and Baumeister, Wolfgang and Zimmermann, Richard and Freeze, Hudson H. and Engel, Benjamin D. and Förster, Friedrich}, year={2017}, month=feb }

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