Volta phase plate cryo-EM of the small protein complex Prx3
10.1038/ncomms10534
Crossref journal-article
Springer Science and Business Media LLC
Nature Communications (297)
Abstract

AbstractCryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

Bibliography

Khoshouei, M., Radjainia, M., Phillips, A. J., Gerrard, J. A., Mitra, A. K., Plitzko, J. M., Baumeister, W., & Danev, R. (2016). Volta phase plate cryo-EM of the small protein complex Prx3. Nature Communications, 7(1).

Authors 8
  1. Maryam Khoshouei (first)
  2. Mazdak Radjainia (additional)
  3. Amy J. Phillips (additional)
  4. Juliet A. Gerrard (additional)
  5. Alok K. Mitra (additional)
  6. Jürgen M. Plitzko (additional)
  7. Wolfgang Baumeister (additional)
  8. Radostin Danev (additional)
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Dates
Type When
Created 9 years, 7 months ago (Jan. 28, 2016, 6:08 a.m.)
Deposited 2 years, 7 months ago (Jan. 4, 2023, 6:52 a.m.)
Indexed 1 year ago (Aug. 3, 2024, 4:16 p.m.)
Issued 9 years, 7 months ago (Jan. 28, 2016)
Published 9 years, 7 months ago (Jan. 28, 2016)
Published Online 9 years, 7 months ago (Jan. 28, 2016)
Funders 0

None

@article{Khoshouei_2016, title={Volta phase plate cryo-EM of the small protein complex Prx3}, volume={7}, ISSN={2041-1723}, url={http://dx.doi.org/10.1038/ncomms10534}, DOI={10.1038/ncomms10534}, number={1}, journal={Nature Communications}, publisher={Springer Science and Business Media LLC}, author={Khoshouei, Maryam and Radjainia, Mazdak and Phillips, Amy J. and Gerrard, Juliet A. and Mitra, Alok K. and Plitzko, Jürgen M. and Baumeister, Wolfgang and Danev, Radostin}, year={2016}, month=jan }