Crossref journal-article
Springer Science and Business Media LLC
Nature Cell Biology (297)
Bibliography

Patla, I., Volberg, T., Elad, N., Hirschfeld-Warneken, V., Grashoff, C., Fässler, R., Spatz, J. P., Geiger, B., & Medalia, O. (2010). Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nature Cell Biology, 12(9), 909–915.

Authors 9
  1. Israel Patla (first)
  2. Tova Volberg (additional)
  3. Nadav Elad (additional)
  4. Vera Hirschfeld-Warneken (additional)
  5. Carsten Grashoff (additional)
  6. Reinhard Fässler (additional)
  7. Joachim P. Spatz (additional)
  8. Benjamin Geiger (additional)
  9. Ohad Medalia (additional)
References 47 Referenced 203
  1. Abercrombie, M. & Dunn, G. A. Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy. Exp. Cell. Res. 92, 57–62 (1975). (10.1016/0014-4827(75)90636-9) / Exp. Cell. Res. by M Abercrombie (1975)
  2. Wiesner, S., Lange, A. & Fässler, R. Local call: from integrins to actin assembly. Trends Cell Biol. 16, 327–329 (2006). (10.1016/j.tcb.2006.05.002) / Trends Cell Biol. by S Wiesner (2006)
  3. Ginsberg, M., Pierschbacher, M. D., Ruoslahti, E., Marguerie, G. & Plow, E. Inhibition of fibronectin binding to platelets by proteolytic fragments and synthetic peptides which support fibroblast adhesion. J. Biol. Chem. 260, 3931–3936 (1985). (10.1016/S0021-9258(18)89211-0) / J. Biol. Chem. by M Ginsberg (1985)
  4. Burridge, K., Fath, K., Kelly, T., Nuckolls, G. & Turner, C. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol. 4, 487–525 (1988). (10.1146/annurev.cb.04.110188.002415) / Annu. Rev. Cell Biol. by K Burridge (1988)
  5. Campbell, I. D. Studies of focal adhesion assembly. Biochem. Soc. Trans. 36, 263–266 (2008). (10.1042/BST0360263) / Biochem. Soc. Trans. by ID Campbell (2008)
  6. Zamir, E. & Geiger, B. Molecular complexity and dynamics of cell–matrix adhesions. J. Cell Sci. 114, 3583–3590 (2001). (10.1242/jcs.114.20.3583) / J. Cell Sci. by E Zamir (2001)
  7. Zaidel-Bar, R., Itzkovitz, S., Ma'ayan, A., Iyengar, R. & Geiger, B. Functional atlas of the integrin adhesome. Nat. Cell Biol. 9, 858–867 (2007). (10.1038/ncb0807-858) / Nat. Cell Biol. by R Zaidel-Bar (2007)
  8. Legate, K. R., Wickstrom, S. A. & Fässler, R. Genetic and cell biological analysis of integrin outside–in signaling. Genes Dev. 23, 397–418 (2009). (10.1101/gad.1758709) / Genes Dev. by KR Legate (2009)
  9. Geiger, B., Spatz, J. P. & Bershadsky, A. D. Environmental sensing through focal adhesions. Nat. Rev. Mol. Cell Biol. 10, 21–33 (2009). (10.1038/nrm2593) / Nat. Rev. Mol. Cell Biol. by B Geiger (2009)
  10. Winograd-Katz, S. E., Itzkovitz, S., Kam, Z. & Geiger, B. Multiparametric analysis of focal adhesion formation by RNAi-mediated gene knockdown. J. Cell Biol. 186, 423–436 (2009). (10.1083/jcb.200901105) / J. Cell Biol. by SE Winograd-Katz (2009)
  11. Delon, I. & Brown, N. H. Integrins and the actin cytoskeleton. Curr. Opin. Cell Biol. 19, 43–50 (2007). (10.1016/j.ceb.2006.12.013) / Curr. Opin. Cell Biol. by I Delon (2007)
  12. Feltkamp, C. A., Pijnenburg, M. A. & Roos, E. Organization of talin and vinculin in adhesion plaques of wet-cleaved chicken embryo fibroblasts. J. Cell Sci. 100, 579–587 (1991). (10.1242/jcs.100.3.579) / J. Cell Sci. by CA Feltkamp (1991)
  13. Nicol, A. et al. Labeling of structural elements at the ventral plasma membrane of fibroblasts with the immunogold technique. J. Histochem. Cytochem. 35, 499–506 (1987). (10.1177/35.4.2880891) / J. Histochem. Cytochem. by A Nicol (1987)
  14. Rinnerthaler, G., Geiger, B. & Small, J. V. Contact formation during fibroblast locomotion: involvement of membrane ruffles and microtubules. J. Cell Biol. 106, 747–760 (1988). (10.1083/jcb.106.3.747) / J. Cell Biol. by G Rinnerthaler (1988)
  15. Turner, C. E. & Miller, J. T. Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: identification of a vinculin and pp125Fak-binding region. J. Cell Sci. 107, 1583–1591 (1994). (10.1242/jcs.107.6.1583) / J. Cell Sci. by CE Turner (1994)
  16. Salgia, R. et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J. Biol. Chem. 270, 5039–5047 (1995). (10.1074/jbc.270.10.5039) / J. Biol. Chem. by R Salgia (1995)
  17. Medalia, O. et al. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298, 1209–1213 (2002). (10.1126/science.1076184) / Science by O Medalia (2002)
  18. Vogel, V. & Sheetz, M. Local force and geometry sensing regulate cell functions. Nat. Rev. Mol. Cell Biol. 7, 265–275 (2006). (10.1038/nrm1890) / Nat. Rev. Mol. Cell Biol. by V Vogel (2006)
  19. Riveline, D. et al. Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. J. Cell Biol. 153, 1175–1186 (2001). (10.1083/jcb.153.6.1175) / J. Cell Biol. by D Riveline (2001)
  20. Chrzanowska-Wodnicka, M. & Burridge, K. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133, 1403–1415 (1996). (10.1083/jcb.133.6.1403) / J. Cell Biol. by M Chrzanowska-Wodnicka (1996)
  21. Lo, S. H. Focal adhesions: what's new inside. Dev. Biol. 294, 280–291 (2006). (10.1016/j.ydbio.2006.03.029) / Dev. Biol. by SH Lo (2006)
  22. Petit, V. & Thiery, J. P. Focal adhesions: structure and dynamics. Biol. Cell 92, 477–494 (2000). (10.1016/S0248-4900(00)01101-1) / Biol. Cell by V Petit (2000)
  23. Singer, I. I. The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Cell 16, 675–685 (1979). (10.1016/0092-8674(79)90040-0) / Cell by II Singer (1979)
  24. Waterman-Storer, C. M., Salmon, W. C. & Salmon, E. D. Feedback interactions between cell–cell adherens junctions and cytoskeletal dynamics in newt lung epithelial cells. Mol. Biol. Cell 11, 2471–2483 (2000). (10.1091/mbc.11.7.2471) / Mol. Biol. Cell by CM Waterman-Storer (2000)
  25. Nicolas, A., Besser, A. & Safran, S. A. Dynamics of cellular focal adhesions on deformable substrates: consequences for cell force microscopy. Biophys. J. 95, 527–539 (2008). (10.1529/biophysj.107.127399) / Biophys. J. by A Nicolas (2008)
  26. Beningo, K. A. & Wang, Y. L. Flexible substrata for the detection of cellular traction forces. Trends Cell Biol. 12, 79–84 (2002). (10.1016/S0962-8924(01)02205-X) / Trends Cell Biol. by KA Beningo (2002)
  27. Wolfenson, H. et al. A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions. PLoS One 4, e4304 (2009). (10.1371/journal.pone.0004304) / PLoS One by H Wolfenson (2009)
  28. Zamir, E., Geiger, B. & Kam, Z. Quantitative multicolor compositional imaging resolves molecular domains in cell–matrix adhesions. PLoS ONE 3, e1901 (2008). (10.1371/journal.pone.0001901) / PLoS ONE by E Zamir (2008)
  29. Frank, J. Electron Tomography (ed. Frank, J.) 1–13 (Plenum, 1992). (10.1007/978-1-4757-2163-8) / Electron Tomography by J Frank (1992)
  30. van Heel, M. Multivariate statistical classification of noisy images (randomly oriented biological macromolecules). Ultramicroscopy 13, 165–183 (1984). (10.1016/0304-3991(84)90066-4) / Ultramicroscopy by M van Heel (1984)
  31. Wolfenson, H., Henis, Y. I., Geiger, B. & Bershadsky, A. D. The heel and toe of the cell's foot: a multifaceted approach for understanding the structure and dynamics of focal adhesions. Cell Motil. Cytoskeleton 66, 1017–1029 (2009). (10.1002/cm.20410) / Cell Motil. Cytoskeleton by H Wolfenson (2009)
  32. Jimenez, J., Santisteban, A., Carazo, J. M. & Carrascosa, J. L. Computer graphic display method for visualizing three-dimensional biological structures. Science 232, 1113–1115 (1986). (10.1126/science.3754654) / Science by J Jimenez (1986)
  33. Avnur, Z. & Geiger, B. Substrate-attached membranes of cultured cells isolation and characterization of ventral cell membranes and the associated cytoskeleton. J. Mol. Biol. 153, 361–379 (1981). (10.1016/0022-2836(81)90283-7) / J. Mol. Biol. by Z Avnur (1981)
  34. Slot, J. W. & Geuze, H. J. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur. J. Cell Biol. 38, 87–93 (1985). / Eur. J. Cell Biol. by JW Slot (1985)
  35. Schwartz, M. A. & DeSimone, D. W. Cell adhesion receptors in mechanotransduction. Curr. Opin. Cell Biol. 20, 551–556 (2008). (10.1016/j.ceb.2008.05.005) / Curr. Opin. Cell Biol. by MA Schwartz (2008)
  36. Narumiya, S., Ishizaki, T. & Uehata, M. Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 325, 273–284 (2000). (10.1016/S0076-6879(00)25449-9) / Methods Enzymol. by S Narumiya (2000)
  37. Katoh, K., Kano, Y. & Ookawara, S. Rho-kinase dependent organization of stress fibers and focal adhesions in cultured fibroblasts. Genes Cells 12, 623–638 (2007). (10.1111/j.1365-2443.2007.01073.x) / Genes Cells by K Katoh (2007)
  38. Cavalcanti-Adam, E. A. et al. Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands. Biophys. J. 92, 2964–2974 (2007). (10.1529/biophysj.106.089730) / Biophys. J. by EA Cavalcanti-Adam (2007)
  39. Finer, J. T., Simmons, R. M. & Spudich, J. A. Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature 368, 113–119 (1994). (10.1038/368113a0) / Nature by JT Finer (1994)
  40. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006). (10.1126/science.1127344) / Science by E Betzig (2006)
  41. Dubochet, J. et al. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21, 129–228 (1988). (10.1017/S0033583500004297) / Q. Rev. Biophys. by J Dubochet (1988)
  42. Sartori, A. et al. Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. J. Struct. Biol. 160, 135–145 (2007). (10.1016/j.jsb.2007.07.011) / J. Struct. Biol. by A Sartori (2007)
  43. Slot, J. W. & Geuze, H. J. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur. J. Cell Biol. 38, 87–93 (1985). / Eur. J. Cell Biol. by JW Slot (1985)
  44. Avnur, Z. & Geiger, B. Substrate-attached membranes of cultured cells isolation and characterization of ventral cell membranes and the associated cytoskeleton. J. Mol. Biol. 153, 361–379 (1981). (10.1016/0022-2836(81)90283-7) / J. Mol. Biol. by Z Avnur (1981)
  45. Nickell, S. et al. TOM software toolbox: acquisition and analysis for electron tomography. J. Struct. Biol. 149, 227–234 (2005). (10.1016/j.jsb.2004.10.006) / J. Struct. Biol. by S Nickell (2005)
  46. Frangakis, A. S. & Hegerl, R. Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion. J. Struct. Biol. 135, 239–250 (2001). (10.1006/jsbi.2001.4406) / J. Struct. Biol. by AS Frangakis (2001)
  47. Hegerl, R. The EM Program Package: a platform for image processing in biological electron microscopy. J. Struct. Biol. 116, 30–34 (1996). (10.1006/jsbi.1996.0006) / J. Struct. Biol. by R Hegerl (1996)
Dates
Type When
Created 15 years ago (Aug. 8, 2010, 2 p.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 3:34 p.m.)
Indexed 2 months ago (June 23, 2025, 10:04 a.m.)
Issued 15 years ago (Aug. 8, 2010)
Published 15 years ago (Aug. 8, 2010)
Published Online 15 years ago (Aug. 8, 2010)
Published Print 14 years, 11 months ago (Sept. 1, 2010)
Funders 0

None

@article{Patla_2010, title={Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography}, volume={12}, ISSN={1476-4679}, url={http://dx.doi.org/10.1038/ncb2095}, DOI={10.1038/ncb2095}, number={9}, journal={Nature Cell Biology}, publisher={Springer Science and Business Media LLC}, author={Patla, Israel and Volberg, Tova and Elad, Nadav and Hirschfeld-Warneken, Vera and Grashoff, Carsten and Fässler, Reinhard and Spatz, Joachim P. and Geiger, Benjamin and Medalia, Ohad}, year={2010}, month=aug, pages={909–915} }