Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins
10.1038/ncb0102-e15
Crossref journal-article
Springer Science and Business Media LLC
Nature Cell Biology (297)
Bibliography

van Roessel, P., & Brand, A. H. (2002). Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nature Cell Biology, 4(1), E15–E20.

Authors 2
  1. Peter van Roessel (first)
  2. Andrea H. Brand (additional)
References 82 Referenced 193
  1. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W. & Prasher, D. C. Green fluorescent protein as a marker for gene expression. Science 263, 802–805 (1994). (10.1126/science.8303295) / Science by M Chalfie (1994)
  2. Heim, R. & Tsien, R. Y. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6, 178–182 (1996). (10.1016/S0960-9822(02)00450-5) / Curr. Biol. by R Heim (1996)
  3. Patterson, G., Day, R. & Piston, D. Fluorescent protein spectra. J. Cell Sci. 114, 837–838 (2001). (10.1242/jcs.114.5.837) / J. Cell Sci. by G Patterson (2001)
  4. Matz, M. V. et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnol. 17, 969–973 (1999). (10.1038/13657) / Nature Biotechnol. by MV Matz (1999)
  5. Baird, G. S., Zacharias, D. A. & Tsien, R. Y. Biochemistry, mutagenesis and oligomerization of DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 97, 11984–11989 (2000). (10.1073/pnas.97.22.11984) / Proc. Natl. Acad. Sci. USA by GS Baird (2000)
  6. Rizzuto, R. et al. Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6, 183–188 (1996). (10.1016/S0960-9822(02)00451-7) / Curr. Biol. by R Rizzuto (1996)
  7. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J. F. Two-color green fluorescent protein time-lapse imaging. Biotechniques 25, 838–842, 844–846 (1998). (10.2144/98255bt01) / Biotechniques by J Ellenberg (1998)
  8. Falk, M. M. Connexin-specific distribution within gap junctions revealed in living cells. J. Cell Sci. 113, 4109–4120 (2000). (10.1242/jcs.113.22.4109) / J. Cell Sci. by MM Falk (2000)
  9. Miller, D. M. 3rd et al. Two-color GFP expression system for C. elegans. Biotechniques 26, 914–918, 920–921 (1999). (10.2144/99265rr01) / Biotechniques by DM Miller 3rd (1999)
  10. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41–51 (2000). (10.1016/S0896-6273(00)00084-2) / Neuron by G Feng (2000)
  11. Hawley, T. S., Telford, W. G. & Hawley, R. G. 'Rainbow' reporters for multispectral marking and lineage analysis of hematopoietic stem cells. Stem Cells 19, 118–124 (2001). (10.1634/stemcells.19-2-118) / Stem Cells by TS Hawley (2001)
  12. Tsukamoto, T. et al. Visualization of gene activity in living cells. Nature Cell Biol. 2, 871–878 (2000). (10.1038/35046510) / Nature Cell Biol. by T Tsukamoto (2000)
  13. Li, X. et al. Generation of destabilized green fluorescent protein as a transcription reporter. J. Biol. Chem. 273, 34970–34975 (1998). (10.1074/jbc.273.52.34970) / J. Biol. Chem. by X Li (1998)
  14. Dantuma, N. P., Lindsten, K., Glas, R., Jellne, M. & Masucci, M. G. Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells. Nature Biotechnol. 18, 538–543 (2000). (10.1038/75406) / Nature Biotechnol. by NP Dantuma (2000)
  15. Terskikh, A. et al. 'Fluorescent timer': protein that changes color with time. Science 290, 1585–1588 (2000). (10.1126/science.290.5496.1585) / Science by A Terskikh (2000)
  16. Bellaiche, Y., Gho, M., Kaltschmidt, J. A., Brand, A. H. & Schweisguth, F. Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division. Nature Cell Biol. 3, 50–57 (2001). (10.1038/35050558) / Nature Cell Biol. by Y Bellaiche (2001)
  17. Straight, A. F., Belmont, A. S., Robinett, C. C. & Murray, A. W. GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion. Curr. Biol. 6, 1599–1608 (1996). (10.1016/S0960-9822(02)70783-5) / Curr. Biol. by AF Straight (1996)
  18. Presley, J. F. et al. ER-to-Golgi transport visualized in living cells. Nature 389, 81–85 (1997). (10.1038/38001) / Nature by JF Presley (1997)
  19. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M. & Pozzan, T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635–642 (1995). (10.1016/S0960-9822(95)00128-X) / Curr. Biol. by R Rizzuto (1995)
  20. Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999). (10.1016/S0896-6273(00)80701-1) / Neuron by T Lee (1999)
  21. Litman, P., Amieva, M. R. & Furthmayr, H. Imaging of dynamic changes of the actin cytoskeleton in microextensions of live NIH3T3 cells with a GFP fusion of the F-actin binding domain of Moesin. BMC Cell Biol. 1, 1 (2000), ( http://www.biomedcentral.com/1471-2121/1/1/ ) (10.1186/1471-2121-1-1) / BMC Cell Biol. by P Litman (2000)
  22. Edwards, K. A., Demsky, M., Montague, R. A., Weymouth, N. & Kiehart, D. P. GFP–moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev. Biol. 191, 103–117 (1997). (10.1006/dbio.1997.8707) / Dev. Biol. by KA Edwards (1997)
  23. Murray, M. J., Merritt, D. J., Brand, A. H. & Whitington, P. M. In vivo dynamics of axon pathfinding in the Drosophila CNS: a time-lapse study of an identified motor neuron. J. Neurobiol. 37, 607–621 (1998). (10.1002/(SICI)1097-4695(199812)37:4<607::AID-NEU9>3.0.CO;2-Q) / J. Neurobiol. by MJ Murray (1998)
  24. Nonet, M. L. Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein–GFP fusions. J. Neurosci. Methods 89, 33–40 (1999). (10.1016/S0165-0270(99)00031-X) / J. Neurosci. Methods by ML Nonet (1999)
  25. Estes, P. S., Ho, G. L., Narayanan, R. & Ramaswami, M. Synaptic localization and restricted diffusion of a Drosophila neuronal synaptobrevin—green fluorescent protein chimera in vivo. J. Neurogenet. 13, 233–255 (2000). (10.3109/01677060009084496) / J. Neurogenet. by PS Estes (2000)
  26. Wacker, I. et al. Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein. J. Cell Sci. 110, 1453–1463 (1997). (10.1242/jcs.110.13.1453) / J. Cell Sci. by I Wacker (1997)
  27. El Meskini, R. et al. A signal sequence is sufficient for green fluorescent protein to be routed to regulated secretory granules. Endocrinology 142, 864–873 (2001). (10.1210/endo.142.2.7929) / Endocrinology by R El Meskini (2001)
  28. Gonzalez, C. & Bejarano, L. A. Protein traps: using intracellular localization for cloning. Trends Cell Biol. 10, 162–165 (2000). (10.1016/S0962-8924(00)01726-8) / Trends Cell Biol. by C Gonzalez (2000)
  29. Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 1, 287–292 (2000). (10.1093/embo-reports/kvd058) / EMBO Rep. by JC Simpson (2000)
  30. Bertrand, E. et al. Localization of ASH1 mRNA particles in living yeast. Mol. Cell 2, 437–445 (1998). (10.1016/S1097-2765(00)80143-4) / Mol. Cell by E Bertrand (1998)
  31. Beach, D. L., Salmon, E. D. & Bloom, K. Localization and anchoring of mRNA in budding yeast. Curr. Biol. 9, 569–578 (1999). (10.1016/S0960-9822(99)80260-7) / Curr. Biol. by DL Beach (1999)
  32. Rook, M. S., Lu, M. & Kosik, K. S. CaMKIIα 3′ untranslated region-directed mRNA translocation in living neurons: visualization by GFP linkage. J. Neurosci. 20, 6385–6393 (2000). (10.1523/JNEUROSCI.20-17-06385.2000) / J. Neurosci. by MS Rook (2000)
  33. Elliott, G. & O'Hare, P. Intercellular trafficking and protein delivery by a herpesvirus structural protein. Cell 88, 223–233 (1997). (10.1016/S0092-8674(00)81843-7) / Cell by G Elliott (1997)
  34. Entchev, E. V., Schwabedissen, A. & Gonzalez-Gaitan, M. Gradient formation of the TGF-β homolog Dpp. Cell 103, 981–991 (2000). (10.1016/S0092-8674(00)00200-2) / Cell by EV Entchev (2000)
  35. Teleman, A. A. & Cohen, S. M. Dpp gradient formation in the Drosophila wing imaginal disc. Cell 103, 971–980 (2000). (10.1016/S0092-8674(00)00199-9) / Cell by AA Teleman (2000)
  36. Kohara, K., Kitamura, A., Morishima, M. & Tsumoto, T. Activity-dependent transfer of brain-derived neurotrophic factor to postsynaptic neurons. Science 291, 2419–2423 (2001). (10.1126/science.1057415) / Science by K Kohara (2001)
  37. Patterson, G. H., Piston, D. W. & Barisas, B. G. Forster distances between green fluorescent protein pairs. Anal. Biochem. 284, 438–440 (2000). (10.1006/abio.2000.4708) / Anal. Biochem. by GH Patterson (2000)
  38. Stryer, L. Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47, 819–846 (1978). (10.1146/annurev.bi.47.070178.004131) / Annu. Rev. Biochem. by L Stryer (1978)
  39. Suzuki, Y., Yasunaga, T., Ohkura, R., Wakabayashi, T. & Sutoh, K. Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps. Nature 396, 380–383 (1998). (10.1038/24640) / Nature by Y Suzuki (1998)
  40. Janetopoulos, C., Jin, T. & Devreotes, P. Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 291, 2408–2411 (2001). (10.1126/science.1055835) / Science by C Janetopoulos (2001)
  41. Majoul, I., Straub, M., Hell, S. W., Duden, R. & Söling, H.-D. KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET. Dev. Cell 1, 139–153 (2001). (10.1016/S1534-5807(01)00004-1) / Dev. Cell by I Majoul (2001)
  42. De Angelis, D. A., Miesenbock, G., Zemelman, B. V. & Rothman, J. E. PRIM: proximity imaging of green fluorescent protein-tagged polypeptides. Proc. Natl. Acad. Sci. USA 95, 12312–12316 (1998). (10.1073/pnas.95.21.12312) / Proc. Natl. Acad. Sci. USA by DA De Angelis (1998)
  43. Gordon, G. W., Berry, G., Liang, X. H., Levine, B. & Herman, B. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74, 2702–2713 (1998). (10.1016/S0006-3495(98)77976-7) / Biophys. J. by GW Gordon (1998)
  44. Xia, Z. & Liu, Y. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81, 2395–2402 (2001). (10.1016/S0006-3495(01)75886-9) / Biophys. J. by Z Xia (2001)
  45. Gadella, T. W. J. J., Jovin, T. M. & Clegg, R. M. Fluorescence lifetime imaging microscopy (FLIM)—spatial resolution of microstructures on the nanosecond time-scale. Biophys. Chem. 48, 221–239 (1993). (10.1016/0301-4622(93)85012-7) / Biophys. Chem. by TWJJ Gadella (1993)
  46. Ng, T. et al. Imaging protein kinase Cα activation in cells. Science 283, 2085–2089 (1999). (10.1126/science.283.5410.2085) / Science by T Ng (1999)
  47. Wouters, F. S. & Bastiaens, P. I. Fluorescence lifetime imaging of receptor tyrosine kinase activity in cells. Curr. Biol. 9, 1127–1130 (1999). (10.1016/S0960-9822(99)80484-9) / Curr. Biol. by FS Wouters (1999)
  48. Verveer, P. J., Wouters, F. S., Reynolds, A. R. & Bastiaens, P. I. Quantitative imaging of lateral ErbB1 receptor signal propagation in the plasma membrane. Science 290, 1567–1570 (2000). (10.1126/science.290.5496.1567) / Science by PJ Verveer (2000)
  49. Xu, Y., Piston, D. W. & Johnson, C. H. A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. USA 96, 151–156 (1999). (10.1073/pnas.96.1.151) / Proc. Natl. Acad. Sci. USA by Y Xu (1999)
  50. Angers, S. et al. Detection of β2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc. Natl. Acad. Sci. USA 97, 3684–3689 (2000). / Proc. Natl. Acad. Sci. USA by S Angers (2000)
  51. Baubet, V. et al. Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level. Proc. Natl. Acad. Sci. USA 97, 7260–7265 (2000). (10.1073/pnas.97.13.7260) / Proc. Natl. Acad. Sci. USA by V Baubet (2000)
  52. Miyawaki, A. et al. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388, 882–887 (1997). (10.1038/42264) / Nature by A Miyawaki (1997)
  53. Miyawaki, A., Griesbeck, O., Heim, R. & Tsien, R. Y. Dynamic and quantitative Ca2+ measurements using improved CAMeleons. Proc. Natl. Acad. Sci. USA 96, 2135–2140 (1999). (10.1073/pnas.96.5.2135) / Proc. Natl. Acad. Sci. USA by A Miyawaki (1999)
  54. Griesbeck, O., Baird, G. S., Campbell, R. E., Zacharias, D. A. & Tsien, R. Y. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J. Biol. Chem. 276, 29188–29194 (2001). (10.1074/jbc.M102815200) / J. Biol. Chem. by O Griesbeck (2001)
  55. Emmanouilidou, E. et al. Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted CAMeleon. Curr. Biol. 9, 915–918 (1999). (10.1016/S0960-9822(99)80398-4) / Curr. Biol. by E Emmanouilidou (1999)
  56. Allen, G. J. et al. CAMeleon calcium indicator reports cytoplasmic calcium dynamics in Arabidopsis guard cells. Plant J. 19, 735–747 (1999). (10.1046/j.1365-313x.1999.00574.x) / Plant J. by GJ Allen (1999)
  57. Kerr, R. et al. Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans. Neuron 26, 583–594 (2000). (10.1016/S0896-6273(00)81196-4) / Neuron by R Kerr (2000)
  58. Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G. & Tsien, R. Y. Measurement of cytosolic, mitochondrial and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803–6808 (1998). (10.1073/pnas.95.12.6803) / Proc. Natl. Acad. Sci. USA by J Llopis (1998)
  59. Kneen, M., Farinas, J., Li, Y. & Verkman, A. S. Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74, 1591–1599 (1998). (10.1016/S0006-3495(98)77870-1) / Biophys. J. by M Kneen (1998)
  60. Miesenbock, G., De Angelis, D. A. & Rothman, J. E. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192–195 (1998). (10.1038/28190) / Nature by G Miesenbock (1998)
  61. Yuste, R., Miller, R. B., Holthoff, K., Zhang, S. & Miesenbock, G. Synapto-pHluorins: chimeras between pH-sensitive mutants of green fluorescent protein and synaptic vesicle membrane proteins as reporters of neurotransmitter release. Methods Enzymol. 327, 522–546 (2000). (10.1016/S0076-6879(00)27300-X) / Methods Enzymol. by R Yuste (2000)
  62. Kuner, T. & Augustine, G. J. A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Neuron 27, 447–459 (2000). (10.1016/S0896-6273(00)00056-8) / Neuron by T Kuner (2000)
  63. Baird, G. S., Zacharias, D. A. & Tsien, R. Y. Circular permutation and receptor insertion within green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96, 11241–11246 (1999). (10.1073/pnas.96.20.11241) / Proc. Natl. Acad. Sci. USA by GS Baird (1999)
  64. Nagai, T., Sawano, A., Park, E. S. & Miyawaki, A. Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. USA 98, 3197–3202 (2001). (10.1073/pnas.051636098) / Proc. Natl. Acad. Sci. USA by T Nagai (2001)
  65. Nakai, J., Ohkura, M. & Imoto, K. A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein. Nature Biotechnol. 19, 137–141 (2001). (10.1038/84397) / Nature Biotechnol. by J Nakai (2001)
  66. Nagai, Y. et al. A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo. Nature Biotechnol. 18, 313–316 (2000). (10.1038/73767) / Nature Biotechnol. by Y Nagai (2000)
  67. Sato, M., Hida, N., Ozawa, T. & Umezawa, Y. Fluorescent indicators for cyclic GMP based on cyclic GMP-dependent protein kinase Iα and green fluorescent proteins. Anal. Chem. 72, 5918–5924 (2000). (10.1021/ac0006167) / Anal. Chem. by M Sato (2000)
  68. Honda, A. et al. Spatiotemporal dynamics of guanosine 3′,5′-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator. Proc. Natl. Acad. Sci. USA 98, 2437–2442 (2001). (10.1073/pnas.051631298) / Proc. Natl. Acad. Sci. USA by A Honda (2001)
  69. Mochizuki, N. et al. Spatio-temporal images of growth-factor-induced activation of Ras and Rap1. Nature 411, 1065–1068 (2001). (10.1038/35082594) / Nature by N Mochizuki (2001)
  70. Vanderklish, P. W. et al. Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 97, 2253–2258 (2000). (10.1073/pnas.040565597) / Proc. Natl. Acad. Sci. USA by PW Vanderklish (2000)
  71. Xu, X. et al. Detection of programmed cell death using fluorescence energy transfer. Nucleic Acids Res. 26, 2034–2035 (1998). (10.1093/nar/26.8.2034) / Nucleic Acids Res. by X Xu (1998)
  72. Luo, K. Q., Yu, V. C., Pu, Y. & Chang, D. C. Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells. Biochem. Biophys. Res. Commun. 283, 1054–1060 (2001). (10.1006/bbrc.2001.4896) / Biochem. Biophys. Res. Commun. by KQ Luo (2001)
  73. Day, R. N. Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy. Mol. Endocrinol. 12, 1410–1419 (1998). (10.1210/mend.12.9.0168) / Mol. Endocrinol. by RN Day (1998)
  74. Day, R. N., Periasamy, A. & Schaufele, F. Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus. Methods 25, 4–18 (2001). (10.1006/meth.2001.1211) / Methods by RN Day (2001)
  75. Zaccolo, M. et al. A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nature Cell Biol. 2, 25–29 (2000). (10.1038/71345) / Nature Cell Biol. by M Zaccolo (2000)
  76. Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990). (10.1126/science.2321027) / Science by W Denk (1990)
  77. Falk, M. M. & Lauf, U. High resolution, fluorescence deconvolution microscopy and tagging with the autofluorescent tracers CFP, GFP and YFP to study the structural composition of gap junctions in living cells. Microsc. Res. Tech. 52, 251–262 (2001). (10.1002/1097-0029(20010201)52:3<251::AID-JEMT1011>3.0.CO;2-#) / Microsc. Res. Tech. by MM Falk (2001)
  78. Straub, M., Lodemann, P., Holroyd, P., Jahn, R. & Hell, S. W. Live cell imaging by multifocal multiphoton microscopy. Eur. J. Cell Biol. 79, 726–734 (2000). (10.1078/0171-9335-00105) / Eur. J. Cell Biol. by M Straub (2000)
  79. Sako, Y., Minoghchi, S. & Yanagida, T. Single-molecule imaging of EGFR signalling on the surface of living cells. Nature Cell Biol. 2, 168–172 (2000). (10.1038/35004044) / Nature Cell Biol. by Y Sako (2000)
  80. Lansford, R., Bearman, G. & Fraser, S. E. Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy. J. Biomed. Opt. 6, 311–318 (2001). (10.1117/1.1383780) / J. Biomed. Opt. by R Lansford (2001)
  81. Khatchatouriants, A., Lewis, A., Rothman, Z., Loew, L. & Treinin, M. GFP is a selective non-linear optical sensor of electrophysiological processes in Caenorhabditis elegans. Biophys. J. 79, 2345–2352 (2000). (10.1016/S0006-3495(00)76480-0) / Biophys. J. by A Khatchatouriants (2000)
  82. Sakai, R., Repunte-Canonigo, V., Raj, C. D. & Knopfel, T. Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein. Eur. J. Neurosci. 13, 2314–2318 (2001). (10.1046/j.0953-816x.2001.01617.x) / Eur. J. Neurosci. by R Sakai (2001)
Dates
Type When
Created 23 years ago (July 26, 2002, 4:30 a.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 3:58 p.m.)
Indexed 2 weeks, 4 days ago (Aug. 5, 2025, 8:11 a.m.)
Issued 23 years, 7 months ago (Jan. 1, 2002)
Published 23 years, 7 months ago (Jan. 1, 2002)
Published Print 23 years, 7 months ago (Jan. 1, 2002)
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@article{van_Roessel_2002, title={Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins}, volume={4}, ISSN={1476-4679}, url={http://dx.doi.org/10.1038/ncb0102-e15}, DOI={10.1038/ncb0102-e15}, number={1}, journal={Nature Cell Biology}, publisher={Springer Science and Business Media LLC}, author={van Roessel, Peter and Brand, Andrea H.}, year={2002}, month=jan, pages={E15–E20} }