Crossref
journal-article
Springer Science and Business Media LLC
Nature Biotechnology (297)
Authors
8
- Malte Wachsmuth (first)
- Christian Conrad (additional)
- Jutta Bulkescher (additional)
- Birgit Koch (additional)
- Robert Mahen (additional)
- Mayumi Isokane (additional)
- Rainer Pepperkok (additional)
- Jan Ellenberg (additional)
References
49
Referenced
149
-
Digman, M.A. & Gratton, E. Lessons in fluctuation correlation spectroscopy. Annu. Rev. Phys. Chem. 62, 645–668 (2011).
(
10.1146/annurev-physchem-032210-103424
) / Annu. Rev. Phys. Chem. by MA Digman (2011) -
Weidemann, T. & Schwille, P. Dual-color fluorescence cross-correlation spectroscopy with continuous laser excitation in a confocal setup. Methods Enzymol. 518, 43–70 (2013).
(
10.1016/B978-0-12-388422-0.00003-0
) / Methods Enzymol. by T Weidemann (2013) -
Simpson, J.C., Neubrand, V.E., Wiemann, S. & Pepperkok, R. Illuminating the human genome. Histochem. Cell Biol. 115, 23–29 (2001).
(
10.1007/s004180000236
) / Histochem. Cell Biol. by JC Simpson (2001) -
Simpson, J.C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 1, 287–292 (2000).
(
10.1093/embo-reports/kvd058
) / EMBO Rep. by JC Simpson (2000) -
Honda, R., Korner, R. & Nigg, E.A. Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. Mol. Biol. Cell 14, 3325–3341 (2003).
(
10.1091/mbc.e02-11-0769
) / Mol. Biol. Cell by R Honda (2003) -
Mahen, R. et al. Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. Mol. Biol. Cell 25, 3610–3618 (2014).
(
10.1091/mbc.e14-06-1091
) / Mol. Biol. Cell by R Mahen (2014) -
Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E. & Webb, W.W. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16, 1055–1069 (1976).
(
10.1016/S0006-3495(76)85755-4
) / Biophys. J. by D Axelrod (1976) -
Elson, E.L. & Magde, D. Fluorescence correlation spectroscopy. I Conceptual basis and theory. Biopolymers 13, 1–27 (1974).
(
10.1002/bip.1974.360130102
) / Biopolymers by EL Elson (1974) -
Stryer, L. Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47, 819–846 (1978).
(
10.1146/annurev.bi.47.070178.004131
) / Annu. Rev. Biochem. by L Stryer (1978) -
Schwille, P., Meyer-Almes, F.J. & Rigler, R. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys. J. 72, 1878–1886 (1997).
(
10.1016/S0006-3495(97)78833-7
) / Biophys. J. by P Schwille (1997) -
Weidemann, T. et al. Counting nucleosomes in living cells with a combination of fluorescence correlation spectroscopy and confocal imaging. J. Mol. Biol. 334, 229–240 (2003).
(
10.1016/j.jmb.2003.08.063
) / J. Mol. Biol. by T Weidemann (2003) -
Schmidt, U. et al. Assembly and mobility of exon-exon junction complexes in living cells. RNA 15, 862–876 (2009).
(
10.1261/rna.1387009
) / RNA by U Schmidt (2009) -
Kaur, G. et al. Probing transcription factor diffusion dynamics in the living mammalian embryo with photoactivatable fluorescence correlation spectroscopy. Nat. Commun. 4, 1637 (2013).
(
10.1038/ncomms2657
) / Nat. Commun. by G Kaur (2013) -
Müller, K.P. et al. Multiscale analysis of dynamics and interactions of heterochromatin protein 1 by fluorescence fluctuation microscopy. Biophys. J. 97, 2876–2885 (2009).
(
10.1016/j.bpj.2009.08.057
) / Biophys. J. by KP Müller (2009) -
Yu, S.R. et al. Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules. Nature 461, 533–536 (2009).
(
10.1038/nature08391
) / Nature by SR Yu (2009) -
Ries, J. et al. Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy. Opt. Express 18, 11073–11082 (2010).
(
10.1364/OE.18.011073
) / Opt. Express by J Ries (2010) -
Carpenter, A.E. & Sabatini, D.M. Systematic genome-wide screens of gene function. Nat. Rev. Genet. 5, 11–22 (2004).
(
10.1038/nrg1248
) / Nat. Rev. Genet. by AE Carpenter (2004) -
Pepperkok, R. & Ellenberg, J. High-throughput fluorescence microscopy for systems biology. Nat. Rev. Mol. Cell Biol. 7, 690–696 (2006).
(
10.1038/nrm1979
) / Nat. Rev. Mol. Cell Biol. by R Pepperkok (2006) -
Sommer, C. & Gerlich, D.W. Machine learning in cell biology - teaching computers to recognize phenotypes. J. Cell Sci. 126, 5529–5539 (2013).
(
10.1242/jcs.123604
) / J. Cell Sci. by C Sommer (2013) -
Conrad, C. et al. Micropilot: automation of fluorescence microscopy-based imaging for systems biology. Nat. Methods 8, 246–249 (2011).
(
10.1038/nmeth.1558
) / Nat. Methods by C Conrad (2011) -
Hutchins, J.R. et al. Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science 328, 593–599 (2010).
(
10.1126/science.1181348
) / Science by JR Hutchins (2010) -
Wood, C. et al. Fluorescence correlation spectroscopy as tool for high-content-screening in yeast (HCS-FCS). Proc. SPIE 7905, 79050H (2011).
(
10.1117/12.873947
) / Proc. SPIE by C Wood (2011) -
Capoulade, J., Wachsmuth, M., Hufnagel, L. & Knop, M. Quantitative fluorescence imaging of protein diffusion and interaction in living cells. Nat. Biotechnol. 29, 835–839 (2011).
(
10.1038/nbt.1928
) / Nat. Biotechnol. by J Capoulade (2011) -
Sankaran, J., Shi, X., Ho, L.Y., Stelzer, E.H. & Wohland, T. ImFCS: a software for imaging FCS data analysis and visualization. Opt. Express 18, 25468–25481 (2010).
(
10.1364/OE.18.025468
) / Opt. Express by J Sankaran (2010) -
Guo, S.M. et al. Bayesian approach to the analysis of fluorescence correlation spectroscopy data II: application to simulated and in vitro data. Anal. Chem. 84, 3880–3888 (2012).
(
10.1021/ac2034375
) / Anal. Chem. by SM Guo (2012) -
Lewis, J.D. et al. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 69, 905–914 (1992).
(
10.1016/0092-8674(92)90610-O
) / Cell by JD Lewis (1992) -
Will, C.L. et al. Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. EMBO J. 21, 4978–4988 (2002).
(
10.1093/emboj/cdf480
) / EMBO J. by CL Will (2002) -
Ebert, D.H. et al. Activity-dependent phosphorylation of MeCP2 threonine 308 regulates interaction with NCoR. Nature 499, 341–345 (2013).
(
10.1038/nature12348
) / Nature by DH Ebert (2013) -
Ma, Z., Moore, R., Xu, X. & Barber, G.N. DDX24 negatively regulates cytosolic RNA-mediated innate immune signaling. PLoS Pathog. 9, e1003721 (2013).
(
10.1371/journal.ppat.1003721
) / PLoS Pathog. by Z Ma (2013) -
Burrows, A.C., Prokop, J. & Summers, M.K. Skp1-Cul1-F-box ubiquitin ligase (SCF(betaTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry. J. Biol. Chem. 287, 39021–39029 (2012).
(
10.1074/jbc.M112.390328
) / J. Biol. Chem. by AC Burrows (2012) -
Regard, J.B. et al. Verge: a novel vascular early response gene. J. Neurosci. 24, 4092–4103 (2004).
(
10.1523/JNEUROSCI.4252-03.2004
) / J. Neurosci. by JB Regard (2004) -
Tokai, N. et al. Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle. EMBO J. 15, 457–467 (1996).
(
10.1002/j.1460-2075.1996.tb00378.x
) / EMBO J. by N Tokai (1996) -
Beck, M. et al. The quantitative proteome of a human cell line. Mol. Syst. Biol. 7, 549 (2011).
(
10.1038/msb.2011.82
) / Mol. Syst. Biol. by M Beck (2011) -
Milo, R., Jorgensen, P., Moran, U., Weber, G. & Springer, M. BioNumbers–the database of key numbers in molecular and cell biology. Nucleic Acids Res. 38, D750–D753 (2010).
(
10.1093/nar/gkp889
) / Nucleic Acids Res. by R Milo (2010) -
Carmena, M., Wheelock, M., Funabiki, H. & Earnshaw, W.C. The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis. Nat. Rev. Mol. Cell Biol. 13, 789–803 (2012).
(
10.1038/nrm3474
) / Nat. Rev. Mol. Cell Biol. by M Carmena (2012) -
Sampath, S.C. et al. The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Cell 118, 187–202 (2004).
(
10.1016/j.cell.2004.06.026
) / Cell by SC Sampath (2004) -
Romano, A. et al. CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1. J. Cell Biol. 161, 229–236 (2003).
(
10.1083/jcb.200207117
) / J. Cell Biol. by A Romano (2003) -
van der Waal, M.S. et al. Mps1 promotes rapid centromere accumulation of Aurora B. EMBO Rep. 13, 847–854 (2012).
(
10.1038/embor.2012.93
) / EMBO Rep. by MS van der Waal (2012) -
Wang, F. et al. A positive feedback loop involving Haspin and Aurora B promotes CPC accumulation at centromeres in mitosis. Curr. Biol. 21, 1061–1069 (2011).
(
10.1016/j.cub.2011.05.016
) / Curr. Biol. by F Wang (2011) -
Xu, Z. et al. INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization. J. Cell Biol. 187, 637–653 (2009).
(
10.1083/jcb.200906053
) / J. Cell Biol. by Z Xu (2009) -
Hayashi-Takanaka, Y., Yamagata, K., Nozaki, N. & Kimura, H. Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase. J. Cell Biol. 187, 781–790 (2009).
(
10.1083/jcb.200904137
) / J. Cell Biol. by Y Hayashi-Takanaka (2009) -
Kiyomitsu, T., Iwasaki, O., Obuse, C. & Yanagida, M. Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. J. Cell Biol. 188, 791–807 (2010).
(
10.1083/jcb.200908096
) / J. Cell Biol. by T Kiyomitsu (2010) -
Huang, S. Non-genetic heterogeneity of cells in development: more than just noise. Development 136, 3853–3862 (2009).
(
10.1242/dev.035139
) / Development by S Huang (2009) -
Held, M. et al. CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Nat. Methods 7, 747–754 (2010).
(
10.1038/nmeth.1486
) / Nat. Methods by M Held (2010) -
Wurzenberger, C. et al. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J. Cell Biol. 198, 173–183 (2012).
(
10.1083/jcb.201112112
) / J. Cell Biol. by C Wurzenberger (2012) -
Schwille, P., Kummer, S., Heikal, A.A., Moerner, W.E. & Webb, W.W. Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins. Proc. Natl. Acad. Sci. USA 97, 151–156 (2000).
(
10.1073/pnas.97.1.151
) / Proc. Natl. Acad. Sci. USA by P Schwille (2000) -
Wachsmuth, M., Waldeck, W. & Langowski, J. Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy. J. Mol. Biol. 298, 677–689 (2000).
(
10.1006/jmbi.2000.3692
) / J. Mol. Biol. by M Wachsmuth (2000) -
Bancaud, A. et al. Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. EMBO J. 28, 3785–3798 (2009).
(
10.1038/emboj.2009.340
) / EMBO J. by A Bancaud (2009) -
Baum, M., Erdel, F., Wachsmuth, M. & Rippe, K. Retrieving the intracellular topology from multi-scale protein mobility mapping in living cells. Nat. Commun. 5, 4494 (2014).
(
10.1038/ncomms5494
) / Nat. Commun. by M Baum (2014)
Dates
Type | When |
---|---|
Created | 10 years, 5 months ago (March 16, 2015, 11:48 a.m.) |
Deposited | 2 years, 3 months ago (May 18, 2023, 2:30 p.m.) |
Indexed | 2 weeks, 1 day ago (Aug. 21, 2025, 12:59 p.m.) |
Issued | 10 years, 5 months ago (March 16, 2015) |
Published | 10 years, 5 months ago (March 16, 2015) |
Published Online | 10 years, 5 months ago (March 16, 2015) |
Published Print | 10 years, 5 months ago (April 1, 2015) |
@article{Wachsmuth_2015, title={High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells}, volume={33}, ISSN={1546-1696}, url={http://dx.doi.org/10.1038/nbt.3146}, DOI={10.1038/nbt.3146}, number={4}, journal={Nature Biotechnology}, publisher={Springer Science and Business Media LLC}, author={Wachsmuth, Malte and Conrad, Christian and Bulkescher, Jutta and Koch, Birgit and Mahen, Robert and Isokane, Mayumi and Pepperkok, Rainer and Ellenberg, Jan}, year={2015}, month=mar, pages={384–389} }