DOI: 10.1038/nature10641. Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket

10.1038/nature10641

Crossref journal-article

Springer Science and Business Media LLC

Nature (297)

Bibliography

Nakashima, R., Sakurai, K., Yamasaki, S., Nishino, K., & Yamaguchi, A. (2011). Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket. Nature, 480(7378), 565â569.

Authors 5

Ryosuke Nakashima (first)
Keisuke Sakurai (additional)
Seiji Yamasaki (additional)
Kunihiko Nishino (additional)
Akihito Yamaguchi (additional)

References 38 Referenced 293

Ma, D., Cook, D. N., Hearst, J. E. & Nikaido, H. Efflux pumps and drug resistance in Gram-negative bacteria. Trends Microbiol. 2, 489–493 (1994) (10.1016/0966-842X(94)90654-8) / Trends Microbiol. by D Ma (1994)
Okusu, H., Ma, D. & Nikaido, H. AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants. J. Bacteriol. 178, 306–308 (1996) (10.1128/jb.178.1.306-308.1996) / J. Bacteriol. by H Okusu (1996)
Paulsen, I. T., Nguyen, L., Sliwinski, M. K., Rabus, R. & Saier, M. H., Jr Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes. J. Mol. Biol. 301, 75–100 (2000) (10.1006/jmbi.2000.3961) / J. Mol. Biol. by IT Paulsen (2000)
Nishino, K. & Yamaguchi, A. Analysis of a complete library of putative drug transporter genes in Escherichia coli. J. Bacteriol. 183, 5803–5812 (2001) (10.1128/JB.183.20.5803-5812.2001) / J. Bacteriol. by K Nishino (2001)
Poole, K. Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms. J. Mol. Microbiol. Biotechnol. 3, 255–264 (2001) / J. Mol. Microbiol. Biotechnol. by K Poole (2001)
Nishino, K., Latifi, T. & Groisman, E. A. Virulence and drug resistance roles of multidrug efflux systems of Salmonera enterica serovar Typhimurium. Mol. Microbiol. 59, 126–141 (2006) (10.1111/j.1365-2958.2005.04940.x) / Mol. Microbiol. by K Nishino (2006)
Poole, K., Krebes, K., McNally, C. & Neshat, S. Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon. J. Bacteriol. 175, 7363–7372 (1993) (10.1128/jb.175.22.7363-7372.1993) / J. Bacteriol. by K Poole (1993)
Sulavik, M. C. et al. Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes. Antimicrob. Agents Chemother. 45, 1126–1136 (2001) (10.1128/AAC.45.4.1126-1136.2001) / Antimicrob. Agents Chemother. by MC Sulavik (2001)
Tikhonova, E. B. & Zgurskaya, H. I. AcrA, AcrB and TolC of Escherichia coli form a stable intermembrane multidrug efflux complex. J. Biol. Chem. 279, 32116–32124 (2004) (10.1074/jbc.M402230200) / J. Biol. Chem. by EB Tikhonova (2004)
Symmons, M. F., Bokma, E., Koronakis, E., Hughes, C. & Koronakis, V. The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc. Natl Acad. Sci. USA 106, 7173–7178 (2009) (10.1073/pnas.0900693106) / Proc. Natl Acad. Sci. USA by MF Symmons (2009)
Fralick, J. A. Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J. Bacteriol. 178, 5803–5805 (1996) (10.1128/jb.178.19.5803-5805.1996) / J. Bacteriol. by JA Fralick (1996)
Koronakis, V., Sharff, A., Koronakis, E., Luisi, B. & Hughes, C. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405, 914–919 (2000) (10.1038/35016007) / Nature by V Koronakis (2000)
Zgurskaya, H. I. & Nikaido, H. AcrA is a highly asymmetric protein capable of spanning the periplasm. J. Mol. Biol. 285, 409–420 (1999) (10.1006/jmbi.1998.2313) / J. Mol. Biol. by HI Zgurskaya (1999)
Mikolosko, J., Bobyk, K., Zgurskaya, H. I. & Ghosh, P. Conformational flexibility in the multidrug efflux system protein AcrA. Structure 14, 577–587 (2006) (10.1016/j.str.2005.11.015) / Structure by J Mikolosko (2006)
Murakami, S., Nakashima, R., Yamashita, E., Matsumoto, T. & Yamaguchi, A. Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature 443, 173–179 (2006) (10.1038/nature05076) / Nature by S Murakami (2006)
Murakami, S., Nakashima, R., Yamashita, E. & Yamaguchi, A. Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419, 587–593 (2002) (10.1038/nature01050) / Nature by S Murakami (2002)
Yu, E. W., McDermott, G., Zgurskaya, H. I., Nikaido, H. & Koshland, D. E., Jr Structural basis of multidrug-binding capacity of the AcrB multidrug efflux pump. Science 300, 976–980 (2003) (10.1126/science.1083137) / Science by EW Yu (2003)
Yu, E. W., Aires, J. R., McDermott, G. & Nikaido, H. A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study. J. Bacteriol. 187, 6804–6815 (2005) (10.1128/JB.187.19.6804-6815.2005) / J. Bacteriol. by EW Yu (2005)
Törnroth-Horsefield, S. et al. Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist. Structure 15, 1663–1673 (2007) (10.1016/j.str.2007.09.023) / Structure by S Törnroth-Horsefield (2007)
Drew, D. et al. The structure of the efflux pump AcrB in complex with bile acid. Mol. Membr. Biol. 25, 677–682 (2008) (10.1080/09687680802552257) / Mol. Membr. Biol. by D Drew (2008)
Pos, K. M., Schiefner, A., Seeger, M. A. & Diederichs, K. Crystallographic analysis of AcrB. FEBS Lett. 564, 333–339 (2004) (10.1016/S0014-5793(04)00272-8) / FEBS Lett. by KM Pos (2004)
Murakami, S. Multidrug efflux transporter, AcrB-the pumping mechanism. Curr. Opin. Struct. Biol. 18, 459–465 (2008) (10.1016/j.sbi.2008.06.007) / Curr. Opin. Struct. Biol. by S Murakami (2008)
Seeger, M. A. et al. Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science 313, 1295–1298 (2006) (10.1126/science.1131542) / Science by MA Seeger (2006)
Seeger, M. A. et al. Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB. Nature Struct. Mol. Biol. 15, 199–205 (2008) (10.1038/nsmb.1379) / Nature Struct. Mol. Biol. by MA Seeger (2008)
Sennhauser, G., Amstutz, P., Briand, C., Storchenegger, O. & Grutter, M. G. Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. PLoS Biol. 5, e7 (2007) (10.1371/journal.pbio.0050007) / PLoS Biol. by G Sennhauser (2007)
Sennhauser, G., Bukowska, M. A., Briand, C. & Grutter, M. G. Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa. J. Mol. Biol. 389, 134–145 (2009) (10.1016/j.jmb.2009.04.001) / J. Mol. Biol. by G Sennhauser (2009)
Schumacher, M. A. et al. Structural mechanisms of QacR induction and multidrug recognition. Science 294, 2158–2163 (2001) (10.1126/science.1066020) / Science by MA Schumacher (2001)
Bohnert, J. A. et al. Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB. J. Bacteriol. 190, 8225–8229 (2008) (10.1128/JB.00912-08) / J. Bacteriol. by JA Bohnert (2008)
Medek, P., Benes, P. & Sochor, J. Computation of tunnels in protein molecules using Delaunay triangulation. J. WSCG 15, 107–114 (2007) / J. WSCG by P Medek (2007)
Pos, K. M. Drug transport mechanism of the AcrB efflux pump. Biochim. Biophys. Acta 1794, 782–793 (2009) (10.1016/j.bbapap.2008.12.015) / Biochim. Biophys. Acta by KM Pos (2009)
Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307–326 (1997) (10.1016/S0076-6879(97)76066-X) / Methods Enzymol. by Z Otwinowski (1997)
Vagin, A. & Teplyakov, A. MOLREP: an automated program for molecular replacement. J. Appl. Crystallogr. 30, 1022–1025 (1997) (10.1107/S0021889897006766) / J. Appl. Crystallogr. by A Vagin (1997)
Collaborative Computational Project. Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D 50, 760–763 (1994) (10.1107/S0907444994003112)
Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 60, 2126–2132 (2004) (10.1107/S0907444904019158) / Acta Crystallogr. D by P Emsley (2004)
Brunger, A. T. Version 1.2 of the crystallography and NMR system. Nature Protocols 2, 2728–2733 (2007) (10.1038/nprot.2007.406) / Nature Protocols by AT Brunger (2007)
Murshudov, G. N., Vagin, A. A. & Dodson, E. J. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr. D 53, 240–255 (1997) (10.1107/S0907444996012255) / Acta Crystallogr. D by GN Murshudov (1997)
Blattner, F. R. et al. The complete genome sequence of Escherichia coli K-12. Science 277, 1453–1462 (1997) (10.1126/science.277.5331.1453) / Science by FR Blattner (1997)
Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177, 4121–4130 (1995) (10.1128/jb.177.14.4121-4130.1995) / J. Bacteriol. by LM Guzman (1995)

Dates

Type	When
Created	13 years, 9 months ago (Nov. 25, 2011, 7:16 a.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 1:56 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 1, 2025, 11:59 p.m.)
Issued	13 years, 9 months ago (Nov. 27, 2011)
Published	13 years, 9 months ago (Nov. 27, 2011)
Published Online	13 years, 9 months ago (Nov. 27, 2011)
Published Print	13 years, 8 months ago (Dec. 1, 2011)

Funders 0

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BibTeX

@article{Nakashima_2011, title={Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket}, volume={480}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature10641}, DOI={10.1038/nature10641}, number={7378}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Nakashima, Ryosuke and Sakurai, Keisuke and Yamasaki, Seiji and Nishino, Kunihiko and Yamaguchi, Akihito}, year={2011}, month=nov, pages={565–569} }

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