Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
10.1038/nature06293
Crossref journal-article
Springer Science and Business Media LLC
Nature (297)
Bibliography

Livet, J., Weissman, T. A., Kang, H., Draft, R. W., Lu, J., Bennis, R. A., Sanes, J. R., & Lichtman, J. W. (2007). Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature, 450(7166), 56–62.

Authors 8
  1. Jean Livet (first)
  2. Tamily A. Weissman (additional)
  3. Hyuno Kang (additional)
  4. Ryan W. Draft (additional)
  5. Ju Lu (additional)
  6. Robyn A. Bennis (additional)
  7. Joshua R. Sanes (additional)
  8. Jeff W. Lichtman (additional)
References 42 Referenced 1,579
  1. Denk, W. & Horstmann, H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol. 2, e329 (2004) (10.1371/journal.pbio.0020329) / PLoS Biol. by W Denk (2004)
  2. Briggman, K. L. & Denk, W. Towards neural circuit reconstruction with volume electron microscopy techniques. Curr. Opin. Neurobiol. 16, 562–570 (2006) (10.1016/j.conb.2006.08.010) / Curr. Opin. Neurobiol. by KL Briggman (2006)
  3. Micheva, K. D. & Smith, S. J. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55, 25–36 (2007) (10.1016/j.neuron.2007.06.014) / Neuron by KD Micheva (2007)
  4. Gan, W. B., Grutzendler, J., Wong, W. T., Wong, R. O. & Lichtman, J. W. Multicolor “DiOlistic” labeling of the nervous system using lipophilic dye combinations. Neuron 27, 219–225 (2000) (10.1016/S0896-6273(00)00031-3) / Neuron by WB Gan (2000)
  5. Shaner, N. C., Steinbach, P. A. & Tsien, R. Y. A guide to choosing fluorescent proteins. Nature Methods 2, 905–909 (2005) (10.1038/nmeth819) / Nature Methods by NC Shaner (2005)
  6. Hadjantonakis, A. K., Macmaster, S. & Nagy, A. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol. 2, 11 (2002) (10.1186/1472-6750-2-11) / BMC Biotechnol. by AK Hadjantonakis (2002)
  7. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41–51 (2000) (10.1016/S0896-6273(00)00084-2) / Neuron by G Feng (2000)
  8. Kasthuri, N. & Lichtman, J. W. The role of neuronal identity in synaptic competition. Nature 424, 426–430 (2003) (10.1038/nature01836) / Nature by N Kasthuri (2003)
  9. Zong, H., Espinosa, J. S., Su, H. H., Muzumdar, M. D. & Luo, L. Mosaic analysis with double markers in mice. Cell 121, 479–492 (2005) (10.1016/j.cell.2005.02.012) / Cell by H Zong (2005)
  10. Branda, C. S. & Dymecki, S. M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev. Cell 6, 7–28 (2004) (10.1016/S1534-5807(03)00399-X) / Dev. Cell by CS Branda (2004)
  11. Lam, K. P. & Rajewsky, K. Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo . Proc. Natl Acad. Sci. USA 95, 13171–13175 (1998) (10.1073/pnas.95.22.13171) / Proc. Natl Acad. Sci. USA by KP Lam (1998)
  12. Lee, G. & Saito, I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene 216, 55–65 (1998) (10.1016/S0378-1119(98)00325-4) / Gene by G Lee (1998)
  13. Caroni, P. Overexpression of growth-associated proteins in the neurons of adult transgenic mice. J. Neurosci. Methods 71, 3–9 (1997) (10.1016/S0165-0270(96)00121-5) / J. Neurosci. Methods by P Caroni (1997)
  14. Guo, C., Yang, W. & Lobe, C. G. A Cre recombinase transgene with mosaic, widespread tamoxifen-inducible action. Genesis 32, 8–18 (2002) (10.1002/gene.10021) / Genesis by C Guo (2002)
  15. Metzger, D., Clifford, J., Chiba, H. & Chambon, P. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc. Natl Acad. Sci. USA 92, 6991–6995 (1995) (10.1073/pnas.92.15.6991) / Proc. Natl Acad. Sci. USA by D Metzger (1995)
  16. Garrick, D., Fiering, S., Martin, D. I. & Whitelaw, E. Repeat-induced gene silencing in mammals. Nature Genet. 18, 56–59 (1998) (10.1038/ng0198-56) / Nature Genet. by D Garrick (1998)
  17. Palmiter, R. D. & Brinster, R. L. Germ-line transformation of mice. Annu. Rev. Genet. 20, 465–499 (1986) (10.1146/annurev.ge.20.120186.002341) / Annu. Rev. Genet. by RD Palmiter (1986)
  18. Rodriguez, C. I. & Dymecki, S. M. Origin of the precerebellar system. Neuron 27, 475–486 (2000) (10.1016/S0896-6273(00)00059-3) / Neuron by CI Rodriguez (2000)
  19. Rowan, S. & Cepko, C. L. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev. Biol. 271, 388–402 (2004) (10.1016/j.ydbio.2004.03.039) / Dev. Biol. by S Rowan (2004)
  20. Eccles, J. C., Ito, M. & Szentágothai, J. in The Cerebellum as a Neuronal Machine (Springer, Berlin, 1967) (10.1007/978-3-662-13147-3) / The Cerebellum as a Neuronal Machine by JC Eccles (1967)
  21. Wu, H. S., Sugihara, I. & Shinoda, Y. Projection patterns of single mossy fibers originating from the lateral reticular nucleus in the rat cerebellar cortex and nuclei. J. Comp. Neurol. 411, 97–118 (1999) (10.1002/(SICI)1096-9861(19990816)411:1<97::AID-CNE8>3.0.CO;2-O) / J. Comp. Neurol. by HS Wu (1999)
  22. Palay, S. L. & Chan-Palay, V. in Cerebellar Cortex (Springer, New York, 1974) (10.1007/978-3-642-65581-4) / Cerebellar Cortex by SL Palay (1974)
  23. Bushong, E. A., Martone, M. E., Jones, Y. Z. & Ellisman, M. H. Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains. J. Neurosci. 22, 183–192 (2002) (10.1523/JNEUROSCI.22-01-00183.2002) / J. Neurosci. by EA Bushong (2002)
  24. Ogata, K. & Kosaka, T. Structural and quantitative analysis of astrocytes in the mouse hippocampus. Neuroscience 113, 221–233 (2002) (10.1016/S0306-4522(02)00041-6) / Neuroscience by K Ogata (2002)
  25. Silver, D. P. & Livingston, D. M. Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity. Mol. Cell 8, 233–243 (2001) (10.1016/S1097-2765(01)00295-7) / Mol. Cell by DP Silver (2001)
  26. Gong, S. et al. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425, 917–925 (2003) (10.1038/nature02033) / Nature by S Gong (2003)
  27. Lein, E. S. et al. Genome-wide atlas of gene expression in the adult mouse brain. Nature 445, 168–176 (2007) (10.1038/nature05453) / Nature by ES Lein (2007)
  28. Zacharias, D. A., Violin, J. D., Newton, A. C. & Tsien, R. Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913–916 (2002) (10.1126/science.1068539) / Science by DA Zacharias (2002)
  29. Rizzo, M. A., Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnol. 22, 445–449 (2004) (10.1038/nbt945) / Nature Biotechnol. by MA Rizzo (2004)
  30. Karasawa, S., Araki, T., Nagai, T., Mizuno, H. & Miyawaki, A. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381, 307–312 (2004) (10.1042/BJ20040321) / Biochem. J. by S Karasawa (2004)
  31. Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl Acad. Sci. USA 99, 7877–7882 (2002) (10.1073/pnas.082243699) / Proc. Natl Acad. Sci. USA by RE Campbell (2002)
  32. Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnol. 22, 1567–1572 (2004) (10.1038/nbt1037) / Nature Biotechnol. by NC Shaner (2004)
  33. Kay, J. N. et al. Transient requirement for ganglion cells during assembly of retinal synaptic layers. Development 131, 1331–1342 (2004) (10.1242/dev.01040) / Development by JN Kay (2004)
  34. Buffelli, M. et al. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424, 430–434 (2003) (10.1038/nature01844) / Nature by M Buffelli (2003)
  35. Zuo, Y. et al. Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination. J. Neurosci. 24, 10999–11009 (2004) (10.1523/JNEUROSCI.3934-04.2004) / J. Neurosci. by Y Zuo (2004)
  36. Fiala, J. C. Reconstruct: a free editor for serial section microscopy. J. Microsc. 218, 52–61 (2005) (10.1111/j.1365-2818.2005.01466.x) / J. Microsc. by JC Fiala (2005)
  37. Kakoki, M. et al. Altering the expression in mice of genes by modifying their 3′ regions. Dev. Cell 6, 597–606 (2004) (10.1016/S1534-5807(04)00094-2) / Dev. Cell by M Kakoki (2004)
  38. Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1, 4 (2001) (10.1186/1471-213X-1-4) / BMC Dev. Biol. by S Srinivas (2001)
  39. Marquardt, T. et al. Pax6 is required for the multipotent state of retinal progenitor cells. Cell 105, 43–55 (2001) (10.1016/S0092-8674(01)00295-1) / Cell by T Marquardt (2001)
  40. Gorski, J. A. et al. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J. Neurosci. 22, 6309–6314 (2002) (10.1523/JNEUROSCI.22-15-06309.2002) / J. Neurosci. by JA Gorski (2002)
  41. Ringrose, L., Chabanis, S., Angrand, P. O., Woodroofe, C. & Stewart, A. F. Quantitative comparison of DNA looping in vitro and in vivo: chromatin increases effective DNA flexibility at short distances. EMBO J. 18, 6630–6641 (1999) (10.1093/emboj/18.23.6630) / EMBO J. by L Ringrose (1999)
  42. Zheng, B., Sage, M., Sheppeard, E. A., Jurecic, V. & Bradley, A. Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications. Mol. Cell. Biol. 20, 648–655 (2000) (10.1128/MCB.20.2.648-655.2000) / Mol. Cell. Biol. by B Zheng (2000)
Dates
Type When
Created 17 years, 10 months ago (Oct. 31, 2007, 2:10 p.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 2:11 p.m.)
Indexed 10 hours, 41 minutes ago (Sept. 6, 2025, 4:14 p.m.)
Issued 17 years, 10 months ago (Nov. 1, 2007)
Published 17 years, 10 months ago (Nov. 1, 2007)
Published Print 17 years, 10 months ago (Nov. 1, 2007)
Funders 0

None

@article{Livet_2007, title={Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system}, volume={450}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature06293}, DOI={10.1038/nature06293}, number={7166}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Livet, Jean and Weissman, Tamily A. and Kang, Hyuno and Draft, Ryan W. and Lu, Ju and Bennis, Robyn A. and Sanes, Joshua R. and Lichtman, Jeff W.}, year={2007}, month=nov, pages={56–62} }