DOI: 10.1038/nature05135. Stoichiometry and turnover in single, functioning membrane protein complexes

Stoichiometry and turnover in single, functioning membrane protein complexes

10.1038/nature05135

Crossref journal-article

Springer Science and Business Media LLC

Nature (297)

Bibliography

Leake, M. C., Chandler, J. H., Wadhams, G. H., Bai, F., Berry, R. M., & Armitage, J. P. (2006). Stoichiometry and turnover in single, functioning membrane protein complexes. Nature, 443(7109), 355â358.

Authors 6

Mark C. Leake (first)
Jennifer H. Chandler (additional)
George H. Wadhams (additional)
Fan Bai (additional)
Richard M. Berry (additional)
Judith P. Armitage (additional)

References 27 Referenced 560

Macnab, R. M. in Escherichia coli and Salmonella: Cellular and Molecular Biology (ed. Neidhardt, F. C.) 123–145 (American Society for Microbiology, Washington DC, 1996) / Escherichia coli and Salmonella: Cellular and Molecular Biology by RM Macnab (1996)
Berry, R. M. & Armitage, J. P. The bacterial flagella motor. Adv. Microb. Physiol. 41, 291–337 (1999) (10.1016/S0065-2911(08)60169-1) / Adv. Microb. Physiol. by RM Berry (1999)
Berg, H. C. The rotary motor of bacterial flagella. Annu. Rev. Biochem. 72, 19–54 (2003) (10.1146/annurev.biochem.72.121801.161737) / Annu. Rev. Biochem. by HC Berg (2003)
Block, S. M. & Berg, H. C. Successive incorporation of force-generating units in the bacterial rotary motor. Nature 309, 470–472 (1984) (10.1038/309470a0) / Nature by SM Block (1984)
Blair, D. F. & Berg, H. C. Restoration of torque in defective flagellar motors. Science 242, 1678–1681 (1988) (10.1126/science.2849208) / Science by DF Blair (1988)
Silverman, M. & Simon, M. Flagellar rotation and the mechanism of bacterial motility. Nature 249, 73–74 (1974) (10.1038/249073a0) / Nature by M Silverman (1974)
Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509–544 (1998) (10.1146/annurev.biochem.67.1.509) / Annu. Rev. Biochem. by RY Tsien (1998)
Mashanov, G. I., Tacon, D., Peckham, M. & Molloy, J. E. The spatial and temporal dynamics of pleckstrin homology domain binding at the plasma membrane measured by imaging single molecules in live mouse myoblasts. J. Biol. Chem. 279, 15274–15280 (2004) (10.1074/jbc.M312140200) / J. Biol. Chem. by GI Mashanov (2004)
Mullineaux, C. W. & Sarcina, M. Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching. Trends Plant Sci. 7, 237–240 (2002) (10.1016/S1360-1385(02)02283-5) / Trends Plant Sci. by CW Mullineaux (2002)
Ray, N., Nenninger, A., Mullineaux, C. W. & Robinson, C. Location and mobility of twin-arginine translocase subunits in the Escherichia coli plasma membrane. J. Biol. Chem. 280, 17961–17968 (2005) (10.1074/jbc.M413521200) / J. Biol. Chem. by N Ray (2005)
Goodwin, J. S. & Kenworthy, A. K. Photobleaching approaches to investigate diffusional mobility and trafficking of Ras in living cells. Methods 37, 154–164 (2005) (10.1016/j.ymeth.2005.05.013) / Methods by JS Goodwin (2005)
Mullineaux, C. W., Nenninger, A., Ray, N. & Robinson, C. Diffusion of green fluorescent protein in three cell environments in Escherichia coli. J. Bacteriol. 188, 3442–3448 (2006) (10.1128/JB.188.10.3442-3448.2006) / J. Bacteriol. by CW Mullineaux (2006)
Berry, R. M., Turner, L. & Berg, H. C. Mechanical limits of bacterial flagellar motors probed by electrorotation. Biophys. J. 69, 280–286 (1995) (10.1016/S0006-3495(95)79900-3) / Biophys. J. by RM Berry (1995)
Khan, S., Dapice, M. & Reese, T. S. Effects of mot gene expression on the structure of the flagellar motor. J. Mol. Biol. 202, 575–584 (1988) (10.1016/0022-2836(88)90287-2) / J. Mol. Biol. by S Khan (1988)
Reid, S. W. et al. The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11. Proc. Natl Acad. Sci. USA 103, 8066–8071 (2006) (10.1073/pnas.0509932103) / Proc. Natl Acad. Sci. USA by SW Reid (2006)
Kojima, S. & Blair, D. F. Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry 43, 26–34 (2004) (10.1021/bi035405l) / Biochemistry by S Kojima (2004)
Sowa, Y. et al. Direct observation of steps in rotation of the bacterial flagellar motor. Nature 437, 916–919 (2005) (10.1038/nature04003) / Nature by Y Sowa (2005)
Sourjik, V. & Berg, H. C. Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions. Mol. Microbiol. 37, 740–751 (2000) (10.1046/j.1365-2958.2000.02044.x) / Mol. Microbiol. by V Sourjik (2000)
Turner, L., Ryu, W. S. & Berg, H. C. Real-time imaging of fluorescent flagellar filaments. J. Bacteriol. 182, 2793–2801 (2000) (10.1128/JB.182.10.2793-2801.2000) / J. Bacteriol. by L Turner (2000)
Dickson, R. M., Cubitt, A. B., Tsien, R. Y. & Moerner, W. E. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388, 355–358 (1997) (10.1038/41048) / Nature by RM Dickson (1997)
Chu, S. Biology and polymer physics at the single-molecule level. Phil. Trans. R. Soc. Lond. A 361, 689–698 (2003) (10.1098/rsta.2002.1157) / Phil. Trans. R. Soc. Lond. A by S Chu (2003)
Leake, M. C., Wilson, D., Gautel, M. & Simmons, R. M. The elasticity of single titin molecules using a two-bead optical tweezers assay. Biophys. J. 87, 1112–1135 (2004) (10.1529/biophysj.103.033571) / Biophys. J. by MC Leake (2004)
Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721–727 (1993) (10.1038/365721a0) / Nature by K Svoboda (1993)
Mashanov, G. I., Tacon, D., Knight, A. E., Peckham, M. & Molloy, J. E. Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy. Methods 29, 142–152 (2003) (10.1016/S1046-2023(02)00305-5) / Methods by GI Mashanov (2003)
Deich, J., Judd, E. M., McAdams, H. H. & Moerner, W. E. Visualization of the movement of single histidine kinase molecules in live Caulobacter cells. Proc. Natl Acad. Sci. USA 101, 15921–15926 (2004) (10.1073/pnas.0404200101) / Proc. Natl Acad. Sci. USA by J Deich (2004)
Van Way, S. M., Hosking, E. R., Braun, T. F. & Manson, M. D. Mot protein assembly into the bacterial flagellum: a model based on mutational analysis of the motB gene. J. Mol. Biol. 297, 7–24 (2000) (10.1006/jmbi.2000.3548) / J. Mol. Biol. by SM Van Way (2000)
Demot, R. & Vanderleyden, J. The C-terminal sequence conservation between OMPA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains peptidoglycan. Mol. Microbiol. 12, 333–336 (1994) (10.1111/j.1365-2958.1994.tb01021.x) / Mol. Microbiol. by R Demot (1994)

Dates

Type	When
Created	18 years, 11 months ago (Sept. 13, 2006, 1:32 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 1:58 p.m.)
Indexed	2 hours, 10 minutes ago (Sept. 6, 2025, 4:23 p.m.)
Issued	19 years ago (Sept. 1, 2006)
Published	19 years ago (Sept. 1, 2006)
Published Online	18 years, 11 months ago (Sept. 13, 2006)
Published Print	19 years ago (Sept. 1, 2006)

Funders 0

None

BibTeX

@article{Leake_2006, title={Stoichiometry and turnover in single, functioning membrane protein complexes}, volume={443}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature05135}, DOI={10.1038/nature05135}, number={7109}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Leake, Mark C. and Chandler, Jennifer H. and Wadhams, George H. and Bai, Fan and Berry, Richard M. and Armitage, Judith P.}, year={2006}, month=sep, pages={355–358} }

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