DOI: 10.1038/nature04719. Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography

Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography

10.1038/nature04719

Crossref journal-article

Springer Science and Business Media LLC

Nature (297)

Bibliography

Liu, J., Taylor, D. W., Krementsova, E. B., Trybus, K. M., & Taylor, K. A. (2006). Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography. Nature, 442(7099), 208â211.

Authors 5

Jun Liu (first)
Dianne W. Taylor (additional)
Elena B. Krementsova (additional)
Kathleen M. Trybus (additional)
Kenneth A. Taylor (additional)

References 30 Referenced 189

Reck-Peterson, S. L., Provance, D. W. Jr, Mooseker, M. S. & Mercer, J. A. Class V myosins. Biochim. Biophys. Acta 1496, 36–51 (2000) (10.1016/S0167-4889(00)00007-0) / Biochim. Biophys. Acta by SL Reck-Peterson (2000)
Mehta, A. D. et al. Myosin-V is a processive actin-based motor. Nature 400, 590–593 (1999) (10.1038/23072) / Nature by AD Mehta (1999)
Forkey, J. N., Quinlan, M. E., Shaw, M. A., Corrie, J. E. & Goldman, Y. E. Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization. Nature 422, 399–404 (2003) (10.1038/nature01529) / Nature by JN Forkey (2003)
Yildiz, A. et al. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 300, 2061–2065 (2003) (10.1126/science.1084398) / Science by A Yildiz (2003)
Wang, F. et al. Effect of ADP and ionic strength on the kinetic and motile properties of recombinant mouse myosin V. J. Biol. Chem. 275, 4329–4335 (2000) (10.1074/jbc.275.6.4329) / J. Biol. Chem. by F Wang (2000)
Homma, K., Saito, J., Ikebe, R. & Ikebe, M. Ca2+-dependent regulation of the motor activity of myosin V. J. Biol. Chem. 275, 34766–34771 (2000) (10.1074/jbc.M003132200) / J. Biol. Chem. by K Homma (2000)
Trybus, K. M., Krementsova, E. & Freyzon, Y. Kinetic characterization of a monomeric unconventional myosin V construct. J. Biol. Chem. 274, 27448–27456 (1999) (10.1074/jbc.274.39.27448) / J. Biol. Chem. by KM Trybus (1999)
Wang, F. et al. Regulated conformation of myosin V. J. Biol. Chem. 279, 2333–2336 (2004) (10.1074/jbc.C300488200) / J. Biol. Chem. by F Wang (2004)
Krementsov, D. N., Krementsova, E. B. & Trybus, K. M. Myosin V: Regulation by calcium, calmodulin, and the tail domain. J. Cell Biol. 164, 877–886 (2004) (10.1083/jcb.200310065) / J. Cell Biol. by DN Krementsov (2004)
Li, X. D., Mabuchi, K., Ikebe, R. & Ikebe, M. Ca2+-induced activation of ATPase activity of myosin Va is accompanied with a large conformational change. Biochem. Biophys. Res. Commun. 315, 538–545 (2004) (10.1016/j.bbrc.2004.01.084) / Biochem. Biophys. Res. Commun. by XD Li (2004)
Frank, J. Three-Dimensional Electron Microscopy of Macromolecular Assemblies (Academic, San Diego, CA, 1996) / Three-Dimensional Electron Microscopy of Macromolecular Assemblies by J Frank (1996)
Terrak, M., Wu, G., Stafford, W. F., Lu, R. C. & Dominguez, R. Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications. EMBO J. 22, 362–371 (2003) (10.1093/emboj/cdg058) / EMBO J. by M Terrak (2003)
Coureux, P. D., Sweeney, H. L. & Houdusse, A. Three myosin V structures delineate essential features of chemo-mechanical transduction. EMBO J. 23, 4527–4537 (2004) (10.1038/sj.emboj.7600458) / EMBO J. by PD Coureux (2004)
Tama, F., Miyashita, O. & Brooks, C. L. Normal mode based flexible fitting of high-resolution structure into low-resolution experimental data from cryo-EM. J. Struct. Biol. 147, 315–326 (2004) (10.1016/j.jsb.2004.03.002) / J. Struct. Biol. by F Tama (2004)
Cheney, R. E. et al. Brain myosin-V is a two-headed unconventional myosin with motor activity. Cell 75, 13–23 (1993) (10.1016/S0092-8674(05)80080-7) / Cell by RE Cheney (1993)
Sweeney, H. L. et al. Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocket. J. Biol. Chem. 273, 6262–6270 (1998) (10.1074/jbc.273.11.6262) / J. Biol. Chem. by HL Sweeney (1998)
Sellers, J. R. Regulation of cytoplasmic and smooth muscle myosin. Curr. Opin. Cell Biol. 3, 98–104 (1991) (10.1016/0955-0674(91)90171-T) / Curr. Opin. Cell Biol. by JR Sellers (1991)
Hackney, D. D., Levitt, J. D. & Suhan, J. Kinesin undergoes a 9 S to 6 S conformational transition. J. Biol. Chem. 267, 8696–8701 (1992) (10.1016/S0021-9258(18)42499-4) / J. Biol. Chem. by DD Hackney (1992)
Wendt, T., Taylor, D., Trybus, K. M. & Taylor, K. Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2. Proc. Natl Acad. Sci. USA 98, 4361–4366 (2001) (10.1073/pnas.071051098) / Proc. Natl Acad. Sci. USA by T Wendt (2001)
Stock, M. F. et al. Formation of the compact confomer of kinesin requires a COOH-terminal heavy chain domain and inhibits microtubule-stimulated ATPase activity. J. Biol. Chem. 274, 14617–14623 (1999) (10.1074/jbc.274.21.14617) / J. Biol. Chem. by MF Stock (1999)
Coy, D. L., Hancock, W. O., Wagenbach, M. & Howard, J. Kinesin's tail domain is an inhibitory regulator of the motor domain. Nature Cell Biol. 1, 288–292 (1999) (10.1038/13001) / Nature Cell Biol. by DL Coy (1999)
Hackney, D. D. & Stock, M. F. Kinesin's IAK tail domain inhibits initial microtubule-stimulated ADP release. Nature Cell Biol. 2, 257–260 (2000) (10.1038/35010525) / Nature Cell Biol. by DD Hackney (2000)
Wang, F. S., Wolenski, J. S., Cheney, R. E., Mooseker, M. S. & Jay, D. G. Function of myosin-V in filopodial extension of neuronal growth cones. Science 273, 660–663 (1996) (10.1126/science.273.5275.660) / Science by FS Wang (1996)
Espreafico, E. M. et al. Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains. J. Cell Biol. 119, 1541–1557 (1992) (10.1083/jcb.119.6.1541) / J. Cell Biol. by EM Espreafico (1992)
Star, E. N., Kwiatkowski, D. J. & Murthy, V. N. Rapid turnover of actin in dendritic spines and its regulation by activity. Nature Neurosci. 5, 239–246 (2002) (10.1038/nn811) / Nature Neurosci. by EN Star (2002)
Forscher, P. & Smith, S. J. Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone. J. Cell Biol. 107, 1505–1516 (1988) (10.1083/jcb.107.4.1505) / J. Cell Biol. by P Forscher (1988)
Ludtke, S. J., Baldwin, P. R. & Chiu, W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128, 82–97 (1999) (10.1006/jsbi.1999.4174) / J. Struct. Biol. by SJ Ludtke (1999)
Winkler, H. & Taylor, K. A. Accurate marker-free alignment with simultaneous geometry determination and reconstruction of tilt series in electron tomography. Ultramicroscopy 106, 240–254 (2006) (10.1016/j.ultramic.2005.07.007) / Ultramicroscopy by H Winkler (2006)
Winkler, H. & Taylor, K. A. Focus gradient correction applied to tilt series image data used in electron tomography. J. Struct. Biol. 143, 24–32 (2003) (10.1016/S1047-8477(03)00120-5) / J. Struct. Biol. by H Winkler (2003)
Rayment, I. et al. Structure of the actin–myosin complex and its implications for muscle contraction. Science 261, 58–65 (1993) (10.1126/science.8316858) / Science by I Rayment (1993)

Dates

Type	When
Created	19 years, 4 months ago (April 19, 2006, 1:40 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 1:54 p.m.)
Indexed	1 month ago (July 26, 2025, 5:08 a.m.)
Issued	19 years, 4 months ago (April 16, 2006)
Published	19 years, 4 months ago (April 16, 2006)
Published Online	19 years, 4 months ago (April 16, 2006)
Published Print	19 years, 1 month ago (July 1, 2006)

Funders 0

None

BibTeX

@article{Liu_2006, title={Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography}, volume={442}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature04719}, DOI={10.1038/nature04719}, number={7099}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Liu, Jun and Taylor, Dianne W. and Krementsova, Elena B. and Trybus, Kathleen M. and Taylor, Kenneth A.}, year={2006}, month=apr, pages={208–211} }

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