Exotoxin A–eEF2 complex structure indicates ADP ribosylation by ribosome mimicry
10.1038/nature03871
Crossref journal-article
Springer Science and Business Media LLC
Nature (297)
Bibliography

Jørgensen, R., Merrill, A. R., Yates, S. P., Marquez, V. E., Schwan, A. L., Boesen, T., & Andersen, G. R. (2005). Exotoxin A–eEF2 complex structure indicates ADP ribosylation by ribosome mimicry. Nature, 436(7053), 979–984.

Authors 7
  1. René Jørgensen (first)
  2. A. Rod Merrill (additional)
  3. Susan P. Yates (additional)
  4. Victor E. Marquez (additional)
  5. Adrian L. Schwan (additional)
  6. Thomas Boesen (additional)
  7. Gregers R. Andersen (additional)
References 38 Referenced 116
  1. Wedekind, J. E. et al. Refined crystallographic structure of Pseudomonas aeruginosa exotoxin A and its implications for the molecular mechanism of toxicity. J. Mol. Biol. 314, 823–837 (2001) (10.1006/jmbi.2001.5195) / J. Mol. Biol. by JE Wedekind (2001)
  2. Li, M., Dyda, F., Benhar, I., Pastan, I. & Davies, D. R. Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation. Proc. Natl Acad. Sci. USA 93, 6902–6906 (1996) (10.1073/pnas.93.14.6902) / Proc. Natl Acad. Sci. USA by M Li (1996)
  3. Weiss, M. S., Blanke, S. R., Collier, R. J. & Eisenberg, D. Structure of the isolated catalytic domain of diphtheria toxin. Biochemistry 34, 773–781 (1995) (10.1021/bi00003a010) / Biochemistry by MS Weiss (1995)
  4. Bell, C. E. & Eisenberg, D. Crystal structure of diphtheria toxin bound to nicotinamide adenine dinucleotide. Biochemistry 35, 1137–1149 (1996) (10.1021/bi9520848) / Biochemistry by CE Bell (1996)
  5. Bell, C. E., Yeates, T. O. & Eisenberg, D. Unusual conformation of nicotinamide adenine dinucleotide (NAD) bound to diphtheria toxin: a comparison with NAD bound to the oxidoreductase enzymes. Protein Sci. 6, 2084–2096 (1997) (10.1002/pro.5560061004) / Protein Sci. by CE Bell (1997)
  6. Han, S., Arvai, A. S., Clancy, S. B. & Tainer, J. A. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J. Mol. Biol. 305, 95–107 (2001) (10.1006/jmbi.2000.4292) / J. Mol. Biol. by S Han (2001)
  7. Menetrey, J. et al. NAD binding induces conformational changes in Rho ADP-ribosylating Clostridium botulinum C3 exoenzyme. J. Biol. Chem. 277, 30950–30957 (2002) (10.1074/jbc.M201844200) / J. Biol. Chem. by J Menetrey (2002)
  8. Evans, H. R. et al. The crystal structure of C3stau2 from Staphylococcus aureus and its complex with NAD. J. Biol. Chem. 278, 45924–45930 (2003) (10.1074/jbc.M307719200) / J. Biol. Chem. by HR Evans (2003)
  9. Han, S., Craig, J. A., Putnam, C. D., Carozzi, N. B. & Tainer, J. A. Evolution and mechanism from structures of an ADP-ribosylating toxin and NAD complex. Nature Struct. Biol. 6, 932–936 (1999) (10.1038/13300) / Nature Struct. Biol. by S Han (1999)
  10. Lyczak, J. B., Cannon, C. L. & Pier, G. B. Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect. 2, 1051–1060 (2000) (10.1016/S1286-4579(00)01259-4) / Microbes Infect. by JB Lyczak (2000)
  11. Mattos-Guaraldi, A. L., Moreira, L. O., Damasco, P. V. & Hirata Junior, R. Diphtheria remains a threat to health in the developing world–an overview. Mem. Inst. Oswaldo Cruz 98, 987–993 (2003) (10.1590/S0074-02762003000800001) / Mem. Inst. Oswaldo Cruz by AL Mattos-Guaraldi (2003)
  12. Van Ness, B. G., Howard, J. B. & Bodley, J. W. ADP-ribosylation of elongation factor 2 by diphtheria toxin. Isolation and properties of the novel ribosyl-amino acid and its hydrolysis products. J. Biol. Chem. 255, 10717–10720 (1980) (10.1016/S0021-9258(19)70366-4) / J. Biol. Chem. by BG Van Ness (1980)
  13. Collier, R. J. Understanding the mode of action of diphtheria toxin: a perspective on progress during the 20th century. Toxicon 39, 1793–1803 (2001) (10.1016/S0041-0101(01)00165-9) / Toxicon by RJ Collier (2001)
  14. Armstrong, S. & Merrill, A. R. Toward the elucidation of the catalytic mechanism of the mono-ADP-ribosyltransferase activity of Pseudomonas aeruginosa exotoxin A. Biochemistry 43, 183–194 (2004) (10.1021/bi034772u) / Biochemistry by S Armstrong (2004)
  15. Beattie, B. K., Prentice, G. A. & Merrill, A. R. Investigation into the catalytic role for the tryptophan residues within domain III of Pseudomonas aeruginosa exotoxin A. Biochemistry 35, 15134–15142 (1996) (10.1021/bi961985t) / Biochemistry by BK Beattie (1996)
  16. Zhou, G. C. et al. Inhibitors of ADP-ribosylating bacterial toxins based on oxacarbenium ion character at their transition states. J. Am. Chem. Soc. 126, 5690–5698 (2004) (10.1021/ja038159+) / J. Am. Chem. Soc. by GC Zhou (2004)
  17. Marquez, V. E. et al. Thiazole-4-carboxamide adenine dinucleotide (TAD). Analogues stable to phosphodiesterase hydrolysis. J. Med. Chem. 29, 1726–1731 (1986) (10.1021/jm00159a027) / J. Med. Chem. by VE Marquez (1986)
  18. Yates, S. P. et al. Structure-function analysis of water soluble inhibitors of the catalytic domain of exotoxin A from Pseudomonas aeruginosa. Biochem. J. 385, 667–675 (2004) (10.1042/BJ20041480) / Biochem. J. by SP Yates (2004)
  19. Jorgensen, R. et al. Two crystal structures demonstrate very large conformational changes of the eukaryotic ribosomal translocase. Nature Struct. Biol. 10, 379–385 (2003) (10.1038/nsb923) / Nature Struct. Biol. by R Jorgensen (2003)
  20. Yates, S. P. & Merrill, A. R. Characterization of oxidized nicotinamide adenine dinucleotide (NAD+) analogues using a high-pressure-liquid-chromatography-based NAD+-glycohydrolase assay and comparison with fluorescence-based measurements. Anal. Biochem. 340, 41–51 (2005) (10.1016/j.ab.2005.01.051) / Anal. Biochem. by SP Yates (2005)
  21. Douglas, C. M. & Collier, R. J. Exotoxin A of Pseudomonas aeruginosa: substitution of glutamic acid 553 with aspartic acid drastically reduces toxicity and enzymatic activity. J. Bacteriol. 169, 4967–4971 (1987) (10.1128/jb.169.11.4967-4971.1987) / J. Bacteriol. by CM Douglas (1987)
  22. Lukac, M. & Collier, R. J. Pseudomonas aeruginosa exotoxin A: effects of mutating tyrosine-470 and tyrosine-481 to phenylalanine. Biochemistry 27, 7629–7632 (1988) (10.1021/bi00420a009) / Biochemistry by M Lukac (1988)
  23. Jorgensen, R. et al. Crystal structure of ADP-ribosylated ribosomal translocase from Saccharomyces cerevisiae. J. Biol. Chem. 279, 45919–45925 (2004) (10.1074/jbc.M406218200) / J. Biol. Chem. by R Jorgensen (2004)
  24. Parikh, S. L. & Schramm, V. L. Transition state structure for ADP-ribosylation of eukaryotic elongation factor 2 catalyzed by diphtheria toxin. Biochemistry 43, 1204–1212 (2004) (10.1021/bi035907z) / Biochemistry by SL Parikh (2004)
  25. Liu, S. & Leppla, S. H. Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins. Mol. Cell 12, 603–613 (2003) (10.1016/j.molcel.2003.08.003) / Mol. Cell by S Liu (2003)
  26. Yates, S. P. & Merrill, A. R. A catalytic loop within Pseudomonas aeruginosa exotoxin A modulates its transferase activity. J. Biol. Chem. 276, 35029–35036 (2001) (10.1074/jbc.M105002200) / J. Biol. Chem. by SP Yates (2001)
  27. Kounnas, M. Z. et al. The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A. J. Biol. Chem. 267, 12420–12423 (1992) (10.1016/S0021-9258(18)42291-0) / J. Biol. Chem. by MZ Kounnas (1992)
  28. Chiron, M. F., Fryling, C. M. & FitzGerald, D. J. Cleavage of pseudomonas exotoxin and diphtheria toxin by a furin-like enzyme prepared from beef liver. J. Biol. Chem. 269, 18167–18176 (1994) (10.1016/S0021-9258(17)32431-6) / J. Biol. Chem. by MF Chiron (1994)
  29. Corda, D. & Di Girolamo, M. Functional aspects of protein mono-ADP-ribosylation. EMBO J. 22, 1953–1958 (2003) (10.1093/emboj/cdg209) / EMBO J. by D Corda (2003)
  30. Spahn, C. M. et al. Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation. EMBO J. 23, 1008–1019 (2004) (10.1038/sj.emboj.7600102) / EMBO J. by CM Spahn (2004)
  31. Ogle, J. M. et al. Recognition of cognate transfer RNA by the 30S ribosomal subunit. Science 292, 897–902 (2001) (10.1126/science.1060612) / Science by JM Ogle (2001)
  32. Jorgensen, R., Carr-Schmid, A., Ortiz, P. A., Kinzy, T. G. & Andersen, G. R. Purification and crystallization of the yeast elongation factor eEF2. Acta Crystallogr. D 58, 712–715 (2002) (10.1107/S0907444902003001) / Acta Crystallogr. D by R Jorgensen (2002)
  33. Armstrong, S., Yates, S. P. & Merrill, A. R. Insight into the catalytic mechanism of Pseudomonas aeruginosa exotoxin A. Studies of toxin interaction with eukaryotic elongation factor-2. J. Biol. Chem. 277, 46669–46675 (2002) (10.1074/jbc.M206916200) / J. Biol. Chem. by S Armstrong (2002)
  34. Vagin, A. & Teplyakov, A. An approach to multi-copy search in molecular replacement. Acta Crystallogr. D 56, 1622–1624 (2000) (10.1107/S0907444900013780) / Acta Crystallogr. D by A Vagin (2000)
  35. Jones, T. A., Cowan, S., Zou, J.-Y. & Kjeldgaard, M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110–119 (1991) (10.1107/S0108767390010224) / Acta Crystallogr. A by TA Jones (1991)
  36. Brunger, A. T. et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905–921 (1998) (10.1107/S0907444998003254) / Acta Crystallogr. D by AT Brunger (1998)
  37. Hayward, S. & Berendsen, H. J. Systematic analysis of domain motions in proteins from conformational change: new results on citrate synthase and T4 lysozyme. Proteins 30, 144–154 (1998) (10.1002/(SICI)1097-0134(19980201)30:2<144::AID-PROT4>3.0.CO;2-N) / Proteins by S Hayward (1998)
  38. DeLano, W. L. The PyMOL User's Manua (DeLano Scientific, San Carlos, 2002) / The PyMOL User's Manua by WL DeLano (2002)
Dates
Type When
Created 20 years ago (Aug. 17, 2005, 1:07 p.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 1:47 p.m.)
Indexed 1 week, 1 day ago (Aug. 28, 2025, 8:17 a.m.)
Issued 20 years, 1 month ago (Aug. 1, 2005)
Published 20 years, 1 month ago (Aug. 1, 2005)
Published Print 20 years, 1 month ago (Aug. 1, 2005)
Funders 0

None

@article{J_rgensen_2005, title={Exotoxin A–eEF2 complex structure indicates ADP ribosylation by ribosome mimicry}, volume={436}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature03871}, DOI={10.1038/nature03871}, number={7053}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Jørgensen, René and Merrill, A. Rod and Yates, Susan P. and Marquez, Victor E. and Schwan, Adrian L. and Boesen, Thomas and Andersen, Gregers R.}, year={2005}, month=aug, pages={979–984} }