Molecular model for a complete clathrin lattice from electron cryomicroscopy
10.1038/nature03079
Crossref journal-article
Springer Science and Business Media LLC
Nature (297)
Bibliography

Fotin, A., Cheng, Y., Sliz, P., Grigorieff, N., Harrison, S. C., Kirchhausen, T., & Walz, T. (2004). Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature, 432(7017), 573–579.

Authors 7
  1. Alexander Fotin (first)
  2. Yifan Cheng (additional)
  3. Piotr Sliz (additional)
  4. Nikolaus Grigorieff (additional)
  5. Stephen C. Harrison (additional)
  6. Tomas Kirchhausen (additional)
  7. Thomas Walz (additional)
References 41 Referenced 459
  1. Kirchhausen, T. Clathrin. Annu. Rev. Biochem. 69, 699–727 (2000) (10.1146/annurev.biochem.69.1.699) / Annu. Rev. Biochem. by T Kirchhausen (2000)
  2. Robinson, M. S. Adaptable adaptors for coated vesicles. Trends Cell Biol. 14, 167–174 (2004) (10.1016/j.tcb.2004.02.002) / Trends Cell Biol. by MS Robinson (2004)
  3. Brodsky, F. M., Chen, C. Y., Knuehl, C., Towler, M. C. & Wakeham, D. E. Biological basket weaving: formation and function of clathrin-coated vesicles. Annu. Rev. Cell Dev. Biol. 17, 517–568 (2001) (10.1146/annurev.cellbio.17.1.517) / Annu. Rev. Cell Dev. Biol. by FM Brodsky (2001)
  4. Crowther, R. A., Finch, J. T. & Pearse, B. M. On the structure of coated vesicles. J. Mol. Biol. 103, 785–798 (1976) (10.1016/0022-2836(76)90209-6) / J. Mol. Biol. by RA Crowther (1976)
  5. Kirchhausen, T. & Harrison, S. C. Protein organization in clathrin trimers. Cell 23, 755–761 (1981) (10.1016/0092-8674(81)90439-6) / Cell by T Kirchhausen (1981)
  6. Ungewickell, E. & Branton, D. Assembly units of clathrin coats. Nature 289, 420–422 (1981) (10.1038/289420a0) / Nature by E Ungewickell (1981)
  7. Kirchhausen, T., Harrison, S. C. & Heuser, J. Configuration of clathrin trimers: Evidence from electron microscopy. J. Ultrastruct. Mol. Struct. Res. 94, 199–208 (1986) (10.1016/0889-1605(86)90067-4) / J. Ultrastruct. Mol. Struct. Res. by T Kirchhausen (1986)
  8. Ungewickell, E. Biochemical and immunological studies on clathrin light chains and their binding sites on clathrin triskelions. EMBO J. 8, 1401–1408 (1983) (10.1002/j.1460-2075.1983.tb01598.x) / EMBO J. by E Ungewickell (1983)
  9. Kirchhausen, T., Harrison, S. C., Parham, P. & Brodsky, F. M. Location and distribution of the light chains in clathrin trimers. Proc. Natl Acad. Sci. USA 80, 2481–2485 (1983) (10.1073/pnas.80.9.2481) / Proc. Natl Acad. Sci. USA by T Kirchhausen (1983)
  10. Kirchhausen, T. et al. Clathrin light chains LCA and LCB are similar, polymorphic and share repeated heptad motifs. Science 236, 320–324 (1987) (10.1126/science.3563513) / Science by T Kirchhausen (1987)
  11. Jackson, A. P., Seow, H. F., Holmes, N., Drickamer, K. & Parham, P. Clathrin light chains contain brain-specific insertion sequences and a region of homology with intermediate filaments. Nature 326, 154–159 (1987) (10.1038/326154a0) / Nature by AP Jackson (1987)
  12. Vigers, G. P., Crowther, R. A. & Pearse, B. M. Three-dimensional structure of clathrin cages in ice. EMBO J. 5, 529–534 (1986) (10.1002/j.1460-2075.1986.tb04242.x) / EMBO J. by GP Vigers (1986)
  13. Vigers, G. P., Crowther, R. A. & Pearse, B. M. Location of the 100 kd-50 kd accessory proteins in clathrin coats. EMBO J. 5, 2079–2085 (1986) (10.1002/j.1460-2075.1986.tb04469.x) / EMBO J. by GP Vigers (1986)
  14. Smith, C. J., Grigorieff, N. & Pearse, B. M. Clathrin coats at 21 Å resolution: a cellular assembly designed to recycle multiple membrane receptors. EMBO J. 17, 4943–4953 (1998) (10.1093/emboj/17.17.4943) / EMBO J. by CJ Smith (1998)
  15. Musacchio, A. et al. Functional organization of clathrin in coats: combining electron cryomicroscopy and X-ray crystallography. Mol. Cell 3, 761–770 (1999) (10.1016/S1097-2765(01)80008-3) / Mol. Cell by A Musacchio (1999)
  16. Ter Haar, E., Musacchio, A., Harrison, S. C. & Kirchhausen, T. Atomic structure of clathrin—a β propeller terminal domain joins an α zigzag linker. Cell 95, 563–573 (1998) (10.1016/S0092-8674(00)81623-2) / Cell by E Ter Haar (1998)
  17. Ybe, J. A. et al. Clathrin self-assembly is mediated by a tandemly repeated superhelix. Nature 399, 371–375 (1999) (10.1038/20708) / Nature by JA Ybe (1999)
  18. Rosenthal, P. B. & Henderson, R. Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. J. Mol. Biol. 333, 721–745 (2003) (10.1016/j.jmb.2003.07.013) / J. Mol. Biol. by PB Rosenthal (2003)
  19. Liu, S.-H., Wong, M. L., Craik, C. S. & Brodsky, F. M. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell 83, 257–267 (1995) (10.1016/0092-8674(95)90167-1) / Cell by S-H Liu (1995)
  20. Kirchhausen, T. & Toyoda, T. Immunoelectron microscopic evidence for the extended conformation of light chains in clathrin trimers. J. Biol. Chem. 268, 10268–10273 (1993) (10.1016/S0021-9258(18)82199-8) / J. Biol. Chem. by T Kirchhausen (1993)
  21. Scarmato, P. & Kirchhausen, T. Analysis of clathrin light chain-heavy chain interactions using truncated mutants of rat liver light chain LCB3. J. Biol. Chem. 265, 3661–3668 (1990) (10.1016/S0021-9258(19)39644-9) / J. Biol. Chem. by P Scarmato (1990)
  22. Nathke, I. S. et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68, 899–910 (1992) (10.1016/0092-8674(92)90033-9) / Cell by IS Nathke (1992)
  23. Chen, C. Y. et al. Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans. EMBO J. 21, 6072–6082 (2002) (10.1093/emboj/cdf594) / EMBO J. by CY Chen (2002)
  24. Ungewickell, E. et al. Role of auxilin in uncoating clathrin-coated vesicles. Nature 378, 632–635 (1995) (10.1038/378632a0) / Nature by E Ungewickell (1995)
  25. Heuser, J. Three-dimensional visualization of coated vesicle formation in fibroblasts. J. Cell Biol. 84, 560–583 (1980) (10.1083/jcb.84.3.560) / J. Cell Biol. by J Heuser (1980)
  26. Ehrlich, M. et al. Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell 118, 591–605 (2004) (10.1016/j.cell.2004.08.017) / Cell by M Ehrlich (2004)
  27. Fotin, A. et al. Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating. Nature doi:10.1038/nature03078 (this issue) (10.1038/nature03078)
  28. Matsui, W. & Kirchhausen, T. Stabilization of clathrin coats by the core of the clathrin-associated protein complex AP-2. Biochemistry 29, 10791–10798 (1990) (10.1021/bi00500a011) / Biochemistry by W Matsui (1990)
  29. Thon, F. Zur Defokussierungsabhängigkeit des Phasenkontrastes bei der elektronenmikroskopischen Abbildung. Z. Naturforsch. 21a, 476–478 (1966) (10.1515/zna-1966-0417) / Z. Naturforsch. by F Thon (1966)
  30. van Heel, M., Harauz, G., Orlova, E. V., Schmidt, R. & Schatz, M. A new generation of the IMAGIC image processing system. J. Struct. Biol. 116, 17–24 (1996) (10.1006/jsbi.1996.0004) / J. Struct. Biol. by M van Heel (1996)
  31. van Heel, M. et al. Single-particle electron cryo-microscopy: towards atomic resolution. Q. Rev. Biophys. 33, 307–369 (2000) (10.1017/S0033583500003644) / Q. Rev. Biophys. by M van Heel (2000)
  32. Mindell, J. A. & Grigorieff, N. Accurate determination of local defocus and specimen tilt in electron microscopy. J. Struct. Biol. 142, 334–347 (2003) (10.1016/S1047-8477(03)00069-8) / J. Struct. Biol. by JA Mindell (2003)
  33. Grigorieff, N. Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 Å in ice. J. Mol. Biol. 277, 1033–1046 (1998) (10.1006/jmbi.1998.1668) / J. Mol. Biol. by N Grigorieff (1998)
  34. Ludtke, S. J., Baldwin, P. R. & Chiu, W. EMAN: semiautomated software for high-resolution single-particle reconstructions. J. Struct. Biol. 128, 82–97 (1999) (10.1006/jsbi.1999.4174) / J. Struct. Biol. by SJ Ludtke (1999)
  35. Huang, C. C., Couch, G. S., Pettersen, E. F. & Ferrin, T. E. Chimera: an extensible molecular modeling application constructed using standard components. Pacif. Symp. Biocomput. 1, 724 (1996) / Pacif. Symp. Biocomput. by CC Huang (1996)
  36. Jones, T. A., Zou, J.-Y. & Cowan, S. W. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110–119 (1991) (10.1107/S0108767390010224) / Acta Crystallogr. A by TA Jones (1991)
  37. Jones, T. A. (ed.) A, yaap, asap, @#*? A Set of Averaging Programs (SERC Daresbury Laboratory, Warrington, 1992)
  38. Kleywegt, G. J. & Jones, T. A. Software for handling macromolecular envelopes. Acta Crystallogr. D 55, 941–944 (1999) (10.1107/S0907444999001031) / Acta Crystallogr. D by GJ Kleywegt (1999)
  39. Marti-Renom, M. A. et al. Comparative protein structure modeling of genes and genomes. Annu. Rev. Biophys. Biomol. Struct. 29, 291–325 (2000) (10.1146/annurev.biophys.29.1.291) / Annu. Rev. Biophys. Biomol. Struct. by MA Marti-Renom (2000)
  40. Sali, A. & Blundell, T. L. Comparative protein modelling by satisfaction of spatial restraints. J. Mol. Biol. 234, 779–815 (1993) (10.1006/jmbi.1993.1626) / J. Mol. Biol. by A Sali (1993)
  41. Collaborative Computational Project. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D50, 760–763 (1994) / Acta Crystallogr. by Collaborative Computational Project (1994)
Dates
Type When
Created 20 years, 9 months ago (Oct. 24, 2004, 1:01 p.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 2:28 p.m.)
Indexed 3 days, 9 hours ago (Aug. 20, 2025, 8:24 a.m.)
Issued 20 years, 9 months ago (Oct. 24, 2004)
Published 20 years, 9 months ago (Oct. 24, 2004)
Published Online 20 years, 9 months ago (Oct. 24, 2004)
Published Print 20 years, 8 months ago (Dec. 1, 2004)
Funders 0

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@article{Fotin_2004, title={Molecular model for a complete clathrin lattice from electron cryomicroscopy}, volume={432}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature03079}, DOI={10.1038/nature03079}, number={7017}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Fotin, Alexander and Cheng, Yifan and Sliz, Piotr and Grigorieff, Nikolaus and Harrison, Stephen C. and Kirchhausen, Tomas and Walz, Thomas}, year={2004}, month=oct, pages={573–579} }