DOI: 10.1038/nature00827. Three-dimensional structure of the bacterial protein-translocation complex SecYEG

Three-dimensional structure of the bacterial protein-translocation complex SecYEG

10.1038/nature00827

Crossref journal-article

Springer Science and Business Media LLC

Nature (297)

Bibliography

Breyton, C., Haase, W., Rapoport, T. A., KÃ¼hlbrandt, W., & Collinson, I. (2002). Three-dimensional structure of the bacterial protein-translocation complex SecYEG. Nature, 418(6898), 662â665.

Authors 5

Cécile Breyton (first)
Winfried Haase (additional)
Tom A. Rapoport (additional)
Werner Kühlbrandt (additional)
Ian Collinson (additional)

References 30 Referenced 211

Matlack, K., Mothes, W. & Rapoport, T. Protein translocation: tunnel vision. Cell 92, 381–390 (1998) (10.1016/S0092-8674(00)80930-7) / Cell by K Matlack (1998)
Brundage, L., Hendrick, J. P., Schiebel, E., Driessen, A. J. & Wickner, W. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 62, 649–657 (1990) (10.1016/0092-8674(90)90111-Q) / Cell by L Brundage (1990)
Akimaru, J., Matsuyama, S. I., Tokuda, H. & Mizushima, S. Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli. Proc. Natl Acad. Sci. USA 88, 6545–6549 (1991) (10.1073/pnas.88.15.6545) / Proc. Natl Acad. Sci. USA by J Akimaru (1991)
Gorlich, D., Prehn, S., Hartmann, E., Kalies, K. & Rapoport, T. A. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Cell 71, 489–503 (1992) (10.1016/0092-8674(92)90517-G) / Cell by D Gorlich (1992)
Hanein, D. et al. Oligomeric ring of the Sec61p complex induced by ligands required for protein translocation. Cell 87, 721–732 (1996) (10.1016/S0092-8674(00)81391-4) / Cell by D Hanein (1996)
Ménétret, J.-F. et al. The structure of ribosome–channel complexes engaged in protein translocation. Mol. Cell 6, 1219–1232 (2000) (10.1016/S1097-2765(00)00118-0) / Mol. Cell by J-F Ménétret (2000)
Beckmann, R. et al. Architecture of the protein-conducting channel associated with the translocating 80S ribosome. Cell 107, 361–372 (2001) (10.1016/S0092-8674(01)00541-4) / Cell by R Beckmann (2001)
Manting, E., van der Does, C., Remigy, H., Engel, A. & Driessen, A. J. M. SecYEG assembles into a tetramer to form the active protein translocation channel. EMBO J. 19, 852–861 (2000) (10.1093/emboj/19.5.852) / EMBO J. by E Manting (2000)
Collinson, I. et al. Projection structure and oligomeric properties of a bacterial core protein translocase. EMBO J. 20, 2462–2471 (2001) (10.1093/emboj/20.10.2462) / EMBO J. by I Collinson (2001)
Nishiyama, K., Suzuki, T. & Tokuda, H. Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell 85, 71–81 (1996) (10.1016/S0092-8674(00)81083-1) / Cell by K Nishiyama (1996)
Akiyama, Y. & Ito, K. Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli. EMBO J. 6, 3465–3470 (1987) (10.1002/j.1460-2075.1987.tb02670.x) / EMBO J. by Y Akiyama (1987)
Duong, F. & Wickner, W. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme. EMBO J. 16, 2756–2768 (1997) (10.1093/emboj/16.10.2756) / EMBO J. by F Duong (1997)
Nishiyama, K., Hanada, M. & Tokuda, H. Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature. EMBO J. 13, 3272–3277 (1994) (10.1002/j.1460-2075.1994.tb06628.x) / EMBO J. by K Nishiyama (1994)
Bessonneau, P., Besson, V., Collinson, I. & Duong, F. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure. EMBO J. 21, 995–1003 (2002) (10.1093/emboj/21.5.995) / EMBO J. by P Bessonneau (2002)
Kaufmann, A., Manting, E. H., Veenendaal, A. K., Driessen, A. J. & van der Does, C. Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighbouring SecE. Biochemistry 38, 9115–9125 (1999) (10.1021/bi990539d) / Biochemistry by A Kaufmann (1999)
Flower, A. M., Osborne, R. S. & Silhavy, T. J. The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE. EMBO J. 14, 884–893 (1995) (10.1002/j.1460-2075.1995.tb07070.x) / EMBO J. by AM Flower (1995)
Plath, K., Mothes, W., Wilkinson, B. M., Stirling, C. J. & Rapoport, T. A. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane. Cell 94, 795–807 (1998) (10.1016/S0092-8674(00)81738-9) / Cell by K Plath (1998)
Veenendaal, A. K., van der Does, C. & Driessen, A. J. Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis. J. Biol. Chem. 276, 32559–32566 (2001) (10.1074/jbc.M103912200) / J. Biol. Chem. by AK Veenendaal (2001)
Economou, A. & Wickner, W. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78, 835–843 (1994) (10.1016/S0092-8674(94)90582-7) / Cell by A Economou (1994)
van der Wolk, J. P., de Wit, J. G. & Driessen, A. J. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events. EMBO J. 16, 7297–7304 (1997) (10.1093/emboj/16.24.7297) / EMBO J. by JP van der Wolk (1997)
Eichler, J., Brunner, J. & Wickner, W. The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase. EMBO J. 16, 2188–2196 (1997) (10.1093/emboj/16.9.2188) / EMBO J. by J Eichler (1997)
Shilton, B. et al. Escherichia coli SecA shape and dimensions. FEBS Lett. 436, 277–282 (1998) (10.1016/S0014-5793(98)01141-7) / FEBS Lett. by B Shilton (1998)
Kleymann, G., Ostermeier, C., Heitmann, K., Haase, W. & Michel, H. Use of antibody fragments (Fv) in immunocytochemistry. J. Histochem. Cytochem. 43, 607–614 (1995) (10.1177/43.6.7769231) / J. Histochem. Cytochem. by G Kleymann (1995)
Fujimoto, K. SDS-digested freeze-fracture replica labeling electron microscopy to study the two-dimensional distribution of integral membrane proteins and phospholipids in biomembranes: practical procedure, interpretation and application. Histochem. Cell Biol. 107, 87–96 (1997) (10.1007/s004180050092) / Histochem. Cell Biol. by K Fujimoto (1997)
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Dates

Type	When
Created	22 years, 11 months ago (Sept. 17, 2002, 8:15 p.m.)
Deposited	2 years, 3 months ago (May 18, 2023, 2:08 p.m.)
Indexed	18 hours, 9 minutes ago (Aug. 22, 2025, 12:45 a.m.)
Issued	23 years ago (Aug. 1, 2002)
Published	23 years ago (Aug. 1, 2002)
Published Print	23 years ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Breyton_2002, title={Three-dimensional structure of the bacterial protein-translocation complex SecYEG}, volume={418}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/nature00827}, DOI={10.1038/nature00827}, number={6898}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Breyton, Cécile and Haase, Winfried and Rapoport, Tom A. and Kühlbrandt, Werner and Collinson, Ian}, year={2002}, month=aug, pages={662–665} }

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