DOI: 10.1038/jcbfm.1995.98. Effects of Central Inhibition of Nitric Oxide Synthase on Focal Cerebral Ischemia in Rats

Effects of Central Inhibition of Nitric Oxide Synthase on Focal Cerebral Ischemia in Rats

10.1038/jcbfm.1995.98

Crossref journal-article

SAGE Publications

Journal of Cerebral Blood Flow & Metabolism (179)

Abstract

We have investigated whether central inhibition of nitric oxide synthase (NOS) could modify the tissue damage of focal cerebral ischemia produced by occlusion of the middle cerebral artery (MCA) in rats. NG-Nitro-l-arginine methyl ester (l-NAME) was administered intracerebroventricularly at two doses 15 min prior to occlusion of the MCA, as well as 4 and 24 h following occlusion. After the injection of l-NAME, the catalytic activity of the constitutive NOS, considered to be mainly neuronal, was effectively suppressed in the subcortical gray matter bilaterally, but not in the ischemic territory. Seven days after the MCA occlusion, the brains were evaluated for histopathologic damage. High-dose administration of l-NAME (120 μg/kg 15 min prior to MCA occlusion, followed by, 150 μg/kg 4 and 24 h after occlusion) produced an enlargement of the infarct area and increased the volume of ischemic damage. These results indicate that extensive inhibition of NOS by a central route can increase the cerebral infarct size in focal ischemia even if NOS is not inhibited in the ischemic tissue and suggest that NO may also play a potentially beneficial role as well as a neurodestructive role in the pathophysiological mechanisms of focal cerebral ischemia.

Bibliography

Hamada, J., Greenberg, J. H., Croul, S., Dawson, T. M., & Reivich, M. (1995). Effects of Central Inhibition of Nitric Oxide Synthase on Focal Cerebral Ischemia in Rats. Journal of Cerebral Blood Flow & Metabolism, 15(5), 779â786.

Authors 5

Junichi Hamada (first)
Joel H. Greenberg (additional)
Sid Croul (additional)
Ted M. Dawson (additional)
Martin Reivich (additional)

References 52 Referenced 29

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{'key': 'bibr18-jcbfm.1995.98', 'first-page': '92', 'volume-title': 'Cerebral Blood Flow and Metabolism', 'author': 'Edvinsson L', 'year': '1993'} / Cerebral Blood Flow and Metabolism by Edvinsson L (1993)
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10.1016/0006-2952(93)90484-E

Dates

Type	When
Created	14 years, 4 months ago (April 8, 2011, 1:21 p.m.)
Deposited	5 months, 2 weeks ago (March 11, 2025, 4:27 a.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:27 a.m.)
Issued	29 years, 11 months ago (Sept. 1, 1995)
Published	29 years, 11 months ago (Sept. 1, 1995)
Published Online	29 years, 11 months ago (Sept. 1, 1995)
Published Print	29 years, 11 months ago (Sept. 1, 1995)

Funders 0

None

BibTeX

@article{Hamada_1995, title={Effects of Central Inhibition of Nitric Oxide Synthase on Focal Cerebral Ischemia in Rats}, volume={15}, ISSN={1559-7016}, url={http://dx.doi.org/10.1038/jcbfm.1995.98}, DOI={10.1038/jcbfm.1995.98}, number={5}, journal={Journal of Cerebral Blood Flow & Metabolism}, publisher={SAGE Publications}, author={Hamada, Junichi and Greenberg, Joel H. and Croul, Sid and Dawson, Ted M. and Reivich, Martin}, year={1995}, month=sep, pages={779–786} }

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