DOI: 10.1038/jcbfm.1993.19. Glial Glycogen Stores Affect Neuronal Survival during Glucose Deprivation in vitro

Glial Glycogen Stores Affect Neuronal Survival during Glucose Deprivation in vitro

10.1038/jcbfm.1993.19

Crossref journal-article

SAGE Publications

Journal of Cerebral Blood Flow & Metabolism (179)

Abstract

Glia perform several energy-dependent functions that may aid neuronal survival under pathological conditions. Glycogen is the major energy reserve in brain, and it is localized almost exclusively to astrocytes. Using murine cortical cell cultures containing both glia and neurons, we examined the effect of altered glial glycogen stores on neuronal survival following glucose deprivation. As previously reported, cultures exposed for several hours to media lacking glucose developed widespread neuronal degeneration without glial degeneration. If glial astrocyte glycogen content was increased to 2–3 times control levels by a 24-h pretreatment with 1 μ M insulin or 0.5 m M methionine sulfoximine (MSO), glucose deprivation-induced neuronal degeneration was attenuated. These protective effects were blocked if glycogen levels were reduced back to control levels by a 30-min exposure to 1 m M dibutyryl cyclic AMP or 20 μ M norepinephrine prior to glucose deprivation. Astrocyte glycogen stores may be an important factor influencing neuronal survival under conditions of energy substrate limitation.

Bibliography

Swanson, R. A., & Choi, D. W. (1993). Glial Glycogen Stores Affect Neuronal Survival during Glucose Deprivation in vitro. Journal of Cerebral Blood Flow & Metabolism, 13(1), 162â169.

Authors 2

Raymond A. Swanson (first)
Dennis W. Choi (additional)

References 56 Referenced 188

10.1111/j.1471-4159.1978.tb10477.x
10.1007/BF01262651
10.1111/j.1471-4159.1971.tb00186.x
10.1016/0166-2236(85)90150-X
10.1002/jemt.1060030406
10.1523/JNEUROSCI.10-08-02493.1990
10.1007/BF02833601
10.1016/0006-8993(86)90529-9
10.1111/j.1528-1157.1987.tb03642.x
{'key': 'bibr10-jcbfm.1993.19', 'first-page': '764', 'volume': '15', 'author': 'Harris KM', 'year': '1989', 'journal-title': 'Soc Neurosci Abstr'} / Soc Neurosci Abstr by Harris KM (1989)
10.1016/0301-0082(79)90018-2
10.1038/jcbfm.1981.17
10.1002/glia.440040108
10.1111/j.1476-5381.1970.tb09551.x
10.1002/glia.440040107
10.1007/BF00965901
10.1016/0165-0270(87)90041-0
10.1523/JNEUROSCI.08-06-02153.1988
10.1016/S0079-6336(74)80003-3
10.1016/S0021-9258(19)52451-6
10.1016/S0021-9258(18)51740-3
10.1126/science.14400
10.1016/0006-8993(89)90179-0
10.1038/jcbfm.1990.62
10.1111/j.1471-4159.1968.tb05904.x
10.1016/S0006-291X(72)80093-7
10.1111/j.1471-4159.1971.tb00187.x
{'key': 'bibr28-jcbfm.1993.19', 'first-page': '133', 'volume-title': 'Dynamic Properties of Glial Cells', 'author': 'Passonneau JV', 'year': '1978'} / Dynamic Properties of Glial Cells by Passonneau JV (1978)
10.1016/0197-0186(86)90025-2
10.1007/BF01261377
10.1210/endo-82-1-1
10.1021/bi00757a001
10.1111/j.1471-4159.1990.tb01194.x
{'key': 'bibr34-jcbfm.1993.19', 'first-page': '46', 'volume-title': 'Methods in Enzymology', 'volume': '1', 'author': 'Rose K', 'year': '1993'} / Methods in Enzymology by Rose K (1993)
10.1016/0165-0270(85)90048-2
10.1126/science.3375817
10.1523/JNEUROSCI.05-01-00023.1985
Siesjö BK. (1978) Brain Energy Metabolism, New York, John Wiley and Sons, pp 196–209
10.1038/jcbfm.1989.20
10.1016/0304-3940(90)90478-R
10.1016/0006-8993(90)90028-A
10.1016/0306-4522(89)90298-4
10.1111/j.1471-4159.1989.tb09180.x
10.1111/j.1471-4159.1990.tb01898.x
10.1161/01.STR.21.2.322
{'key': 'bibr46-jcbfm.1993.19', 'first-page': '543', 'volume': '13', 'author': 'Tews JK', 'year': '1964', 'journal-title': 'Biochem Biophys Res Comm'} / Biochem Biophys Res Comm by Tews JK (1964)
10.1111/j.1471-4159.1992.tb08884.x
10.1073/pnas.85.22.8727
{'key': 'bibr49-jcbfm.1993.19', 'first-page': '1', 'volume': '17', 'author': 'Varon S', 'year': '1979', 'journal-title': 'Neurosci Res Prog Bull'} / Neurosci Res Prog Bull by Varon S (1979)
10.1016/0006-8993(87)90937-1
10.1016/0301-0082(89)90005-1
10.1016/0304-3940(88)90499-5
10.1111/j.1471-4159.1986.tb02863.x
10.1016/0006-8993(91)91222-M
10.1111/j.1471-4159.1988.tb01820.x
10.1016/0304-3940(89)90165-1

Dates

Type	When
Created	14 years, 4 months ago (April 8, 2011, 1:15 p.m.)
Deposited	5 months, 3 weeks ago (March 11, 2025, 5:27 a.m.)
Indexed	2 days, 12 hours ago (Sept. 3, 2025, 7:18 a.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	32 years, 8 months ago (Jan. 1, 1993)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{Swanson_1993, title={Glial Glycogen Stores Affect Neuronal Survival during Glucose Deprivation in vitro}, volume={13}, ISSN={1559-7016}, url={http://dx.doi.org/10.1038/jcbfm.1993.19}, DOI={10.1038/jcbfm.1993.19}, number={1}, journal={Journal of Cerebral Blood Flow & Metabolism}, publisher={SAGE Publications}, author={Swanson, Raymond A. and Choi, Dennis W.}, year={1993}, month=jan, pages={162–169} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        3
      ]
    ],
    "date-time": "2025-09-03T11:18:40Z",
    "timestamp": 1756898320518,
    "version": "3.38.0"
  },
  "reference-count": 56,
  "publisher": "SAGE Publications",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1993,
            1,
            1
          ]
        ],
        "date-time": "1993-01-01T00:00:00Z",
        "timestamp": 725846400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.sagepub.com/page/policies/text-and-data-mining-license"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        1
      ]
    ]
  },
  "abstract": "<jats:p> Glia perform several energy-dependent functions that may aid neuronal survival under pathological conditions. Glycogen is the major energy reserve in brain, and it is localized almost exclusively to astrocytes. Using murine cortical cell cultures containing both glia and neurons, we examined the effect of altered glial glycogen stores on neuronal survival following glucose deprivation. As previously reported, cultures exposed for several hours to media lacking glucose developed widespread neuronal degeneration without glial degeneration. If glial astrocyte glycogen content was increased to 2\u20133 times control levels by a 24-h pretreatment with 1 \u03bc M insulin or 0.5 m M methionine sulfoximine (MSO), glucose deprivation-induced neuronal degeneration was attenuated. These protective effects were blocked if glycogen levels were reduced back to control levels by a 30-min exposure to 1 m M dibutyryl cyclic AMP or 20 \u03bc M norepinephrine prior to glucose deprivation. Astrocyte glycogen stores may be an important factor influencing neuronal survival under conditions of energy substrate limitation. </jats:p>",
  "DOI": "10.1038/jcbfm.1993.19",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2011,
        4,
        8
      ]
    ],
    "date-time": "2011-04-08T17:15:12Z",
    "timestamp": 1302282912000
  },
  "page": "162-169",
  "source": "Crossref",
  "is-referenced-by-count": 188,
  "title": "Glial Glycogen Stores Affect Neuronal Survival during Glucose Deprivation in vitro",
  "prefix": "10.1177",
  "volume": "13",
  "author": [
    {
      "given": "Raymond A.",
      "family": "Swanson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Dennis W.",
      "family": "Choi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Neurology, Washington University School of Medicine, St. Louis, Missouri, U.S.A."
        }
      ]
    }
  ],
  "member": "179",
  "published-online": {
    "date-parts": [
      [
        1993,
        1,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "bibr1-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1978.tb10477.x"
    },
    {
      "key": "bibr2-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01262651"
    },
    {
      "key": "bibr3-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1971.tb00186.x"
    },
    {
      "key": "bibr4-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-2236(85)90150-X"
    },
    {
      "key": "bibr5-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060030406"
    },
    {
      "key": "bibr6-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.10-08-02493.1990"
    },
    {
      "key": "bibr7-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02833601"
    },
    {
      "key": "bibr8-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(86)90529-9"
    },
    {
      "key": "bibr9-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1528-1157.1987.tb03642.x"
    },
    {
      "key": "bibr10-jcbfm.1993.19",
      "first-page": "764",
      "volume": "15",
      "author": "Harris KM",
      "year": "1989",
      "journal-title": "Soc Neurosci Abstr"
    },
    {
      "key": "bibr11-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0301-0082(79)90018-2"
    },
    {
      "key": "bibr12-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1981.17"
    },
    {
      "key": "bibr13-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/glia.440040108"
    },
    {
      "key": "bibr14-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1476-5381.1970.tb09551.x"
    },
    {
      "key": "bibr15-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/glia.440040107"
    },
    {
      "key": "bibr16-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00965901"
    },
    {
      "key": "bibr17-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-0270(87)90041-0"
    },
    {
      "key": "bibr18-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.08-06-02153.1988"
    },
    {
      "key": "bibr19-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6336(74)80003-3"
    },
    {
      "key": "bibr20-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)52451-6"
    },
    {
      "key": "bibr21-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)51740-3"
    },
    {
      "key": "bibr22-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.14400"
    },
    {
      "key": "bibr23-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(89)90179-0"
    },
    {
      "key": "bibr24-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1990.62"
    },
    {
      "key": "bibr25-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1968.tb05904.x"
    },
    {
      "key": "bibr26-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-291X(72)80093-7"
    },
    {
      "key": "bibr27-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1971.tb00187.x"
    },
    {
      "key": "bibr28-jcbfm.1993.19",
      "first-page": "133",
      "volume-title": "Dynamic Properties of Glial Cells",
      "author": "Passonneau JV",
      "year": "1978"
    },
    {
      "key": "bibr29-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0197-0186(86)90025-2"
    },
    {
      "key": "bibr30-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01261377"
    },
    {
      "key": "bibr31-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1210/endo-82-1-1"
    },
    {
      "key": "bibr32-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00757a001"
    },
    {
      "key": "bibr33-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1990.tb01194.x"
    },
    {
      "key": "bibr34-jcbfm.1993.19",
      "first-page": "46",
      "volume-title": "Methods in Enzymology",
      "volume": "1",
      "author": "Rose K",
      "year": "1993"
    },
    {
      "key": "bibr35-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-0270(85)90048-2"
    },
    {
      "key": "bibr36-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3375817"
    },
    {
      "key": "bibr37-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.05-01-00023.1985"
    },
    {
      "key": "bibr38-jcbfm.1993.19",
      "unstructured": "Siesj\u00f6 BK. (1978) Brain Energy Metabolism, New York, John Wiley and Sons, pp 196\u2013209"
    },
    {
      "key": "bibr39-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1989.20"
    },
    {
      "key": "bibr40-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(90)90478-R"
    },
    {
      "key": "bibr41-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(90)90028-A"
    },
    {
      "key": "bibr42-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0306-4522(89)90298-4"
    },
    {
      "key": "bibr43-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1989.tb09180.x"
    },
    {
      "key": "bibr44-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1990.tb01898.x"
    },
    {
      "key": "bibr45-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.STR.21.2.322"
    },
    {
      "key": "bibr46-jcbfm.1993.19",
      "first-page": "543",
      "volume": "13",
      "author": "Tews JK",
      "year": "1964",
      "journal-title": "Biochem Biophys Res Comm"
    },
    {
      "key": "bibr47-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1992.tb08884.x"
    },
    {
      "key": "bibr48-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.22.8727"
    },
    {
      "key": "bibr49-jcbfm.1993.19",
      "first-page": "1",
      "volume": "17",
      "author": "Varon S",
      "year": "1979",
      "journal-title": "Neurosci Res Prog Bull"
    },
    {
      "key": "bibr50-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(87)90937-1"
    },
    {
      "key": "bibr51-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0301-0082(89)90005-1"
    },
    {
      "key": "bibr52-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(88)90499-5"
    },
    {
      "key": "bibr53-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1986.tb02863.x"
    },
    {
      "key": "bibr54-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(91)91222-M"
    },
    {
      "key": "bibr55-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1988.tb01820.x"
    },
    {
      "key": "bibr56-jcbfm.1993.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(89)90165-1"
    }
  ],
  "container-title": "Journal of Cerebral Blood Flow &amp; Metabolism",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.sagepub.com/doi/pdf/10.1038/jcbfm.1993.19",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.sagepub.com/doi/pdf/10.1038/jcbfm.1993.19",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        3,
        11
      ]
    ],
    "date-time": "2025-03-11T09:27:32Z",
    "timestamp": 1741685252000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.sagepub.com/doi/10.1038/jcbfm.1993.19"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        1
      ]
    ]
  },
  "references-count": 56,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1993,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1038/jcbfm.1993.19"
  ],
  "URL": "http://dx.doi.org/10.1038/jcbfm.1993.19",
  "relation": {},
  "ISSN": [
    "0271-678X",
    "1559-7016"
  ],
  "subject": [],
  "container-title-short": "J Cereb Blood Flow Metab",
  "published": {
    "date-parts": [
      [
        1993,
        1
      ]
    ]
  }
}