DOI: 10.1038/jcbfm.1984.26. Extracellular pH, Potassium, and Calcium Activities in Progressive Ischaemia of Rat Cortex

Extracellular pH, Potassium, and Calcium Activities in Progressive Ischaemia of Rat Cortex

10.1038/jcbfm.1984.26

Crossref journal-article

SAGE Publications

Journal of Cerebral Blood Flow & Metabolism (179)

Abstract

We measured the relationships between changes in extracellular pH (pHe), potassium (Ke), and calcium (Cae) activities and DC potential (DCe) in progressive ischaemia of rat cerebral cortex. pHe and Ke, or Cae and Ke, were measured at the same point simultaneously, using triple-barrelled, double-ion-sensitive microelectrodes. Ischaemia was produced using bilateral carotid artery occlusion and hypotension in rats under 50% N2O-0.4% halothane anaesthesia. Unilateral carotid artery occlusion did not affect blood flow, but bilateral occlusion reduced flow to ∼40% of normal. Autoregulation of blood pressure (BP) changes was lost after bilateral occlusion, and so progressive hypotension produced a linear decrease in flow. pHe began to decrease at high levels of flow (30–35 ml 100 g−1 min−1) and showed stepwise acidotic shifs with reductions in BP. Ke was affected at flows of ∼15 ml 100 g−1 min −1, during which time it was critically dependent on BP. When Ke reached 6 m M, it increased rapidly to 40 m M and was associated with a negative shift in DCe. When Ke reached ∼10 m M, Cae decreased rapidly to ∼0.1 m M. pHe had reached 6.87 when Ke increased rapidly and showed a transient alkalotic shift of ∼0.14 units at that time. Possible mechanisms for the sequence of ion changes described are discussed.

Bibliography

Harris, R. J., & Symon, L. (1984). Extracellular pH, Potassium, and Calcium Activities in Progressive Ischaemia of Rat Cortex. Journal of Cerebral Blood Flow & Metabolism, 4(2), 178â186.

Authors 2

Robert J. Harris (first)
Lindsay Symon (additional)

References 43 Referenced 140

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Dates

Type	When
Created	14 years, 4 months ago (April 8, 2011, 1:28 p.m.)
Deposited	5 months, 3 weeks ago (March 10, 2025, 4:33 p.m.)
Indexed	2 days, 17 hours ago (Sept. 3, 2025, 6:39 a.m.)
Issued	41 years, 3 months ago (June 1, 1984)
Published	41 years, 3 months ago (June 1, 1984)
Published Online	41 years, 3 months ago (June 1, 1984)
Published Print	41 years, 3 months ago (June 1, 1984)

Funders 0

None

BibTeX

@article{Harris_1984, title={Extracellular pH, Potassium, and Calcium Activities in Progressive Ischaemia of Rat Cortex}, volume={4}, ISSN={1559-7016}, url={http://dx.doi.org/10.1038/jcbfm.1984.26}, DOI={10.1038/jcbfm.1984.26}, number={2}, journal={Journal of Cerebral Blood Flow & Metabolism}, publisher={SAGE Publications}, author={Harris, Robert J. and Symon, Lindsay}, year={1984}, month=jun, pages={178–186} }

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  "abstract": "<jats:p> We measured the relationships between changes in extracellular pH (pH<jats:sub>e</jats:sub>), potassium (K<jats:sub>e</jats:sub>), and calcium (Ca<jats:sub>e</jats:sub>) activities and DC potential (DC<jats:sub>e</jats:sub>) in progressive ischaemia of rat cerebral cortex. pH<jats:sub>e</jats:sub> and K<jats:sub>e</jats:sub>, or Ca<jats:sub>e</jats:sub> and K<jats:sub>e</jats:sub>, were measured at the same point simultaneously, using triple-barrelled, double-ion-sensitive microelectrodes. Ischaemia was produced using bilateral carotid artery occlusion and hypotension in rats under 50% N<jats:sub>2</jats:sub>O-0.4% halothane anaesthesia. Unilateral carotid artery occlusion did not affect blood flow, but bilateral occlusion reduced flow to \u223c40% of normal. Autoregulation of blood pressure (BP) changes was lost after bilateral occlusion, and so progressive hypotension produced a linear decrease in flow. pH<jats:sub>e</jats:sub> began to decrease at high levels of flow (30\u201335 ml 100 g<jats:sup>\u22121</jats:sup> min<jats:sup>\u22121</jats:sup>) and showed stepwise acidotic shifs with reductions in BP. K<jats:sub>e</jats:sub> was affected at flows of \u223c15 ml 100 g<jats:sup>\u22121</jats:sup> min <jats:sup>\u22121</jats:sup>, during which time it was critically dependent on BP. When K<jats:sub>e</jats:sub> reached 6 m M, it increased rapidly to 40 m M and was associated with a negative shift in DC<jats:sub>e</jats:sub>. When K<jats:sub>e</jats:sub> reached \u223c10 m M, Ca<jats:sub>e</jats:sub> decreased rapidly to \u223c0.1 m M. pH<jats:sub>e</jats:sub> had reached 6.87 when K<jats:sub>e</jats:sub> increased rapidly and showed a transient alkalotic shift of \u223c0.14 units at that time. Possible mechanisms for the sequence of ion changes described are discussed. </jats:p>",
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