DOI: 10.1038/jcbfm.1983.7. Simultaneous Measurement of Local Glucose Utilization and Blood Flow in the Rat Brain: An Autoradiographic Method Using Two Tracers Labeled with Carbon-14

Simultaneous Measurement of Local Glucose Utilization and Blood Flow in the Rat Brain: An Autoradiographic Method Using Two Tracers Labeled with Carbon-14

10.1038/jcbfm.1983.7

Crossref journal-article

SAGE Publications

Journal of Cerebral Blood Flow & Metabolism (179)

Abstract

A simplified technique that uses two radionuclide tracers has been devised to measure local cerebral glucose utilization (lCGU) and local cerebral blood flow (lCBF) in the same rat. The method employs [14C]-2-deoxyglucose and [14C]iodoantipyrine to produce an autoradiogram before and another after extraction into chloroform of the [14C]iodoantipyrine from the brain sections. The chloroform-extracted autoradiogram yields lCGU, and the difference in tissue carbon-14 concentration between the two autoradiograms permits calculation of lCBF. The double-isotope technique provides values of lCGU and lCBF that are statistically indistinguishable from those derived from conventional single-isotope methods.

Bibliography

Furlow, T. W., & Harrison, L. E. (1983). Simultaneous Measurement of Local Glucose Utilization and Blood Flow in the Rat Brain: An Autoradiographic Method Using Two Tracers Labeled with Carbon-14. Journal of Cerebral Blood Flow & Metabolism, 3(1), 62â66.

Authors 2

Thomas W. Furlow (first)
Lynn E. Harrison (additional)

References 11 Referenced 35

{'key': 'bibr1-jcbfm.1983.7', 'first-page': 'S72', 'volume': '1', 'author': 'Diemer NH', 'year': '1981', 'journal-title': 'J Cereb Blood Flow Metabol'} / J Cereb Blood Flow Metabol by Diemer NH (1981)
10.1111/j.1471-4159.1980.tb11239.x
10.1111/j.1399-6576.1976.tb05013.x
10.1038/jcbfm.1982.3
10.1161/01.STR.12.5.589
10.1161/01.STR.12.5.581
10.1159/000115232
10.1159/000115227
{'key': 'bibr9-jcbfm.1983.7', 'first-page': '125', 'volume-title': 'Research Publications for the Association of Nervous and Mental Diseases, Vol. 53: Brain Dysfunction in Metabolic Disorders', 'author': 'Reivich M', 'year': '1974'} / Research Publications for the Association of Nervous and Mental Diseases, Vol. 53: Brain Dysfunction in Metabolic Disorders by Reivich M (1974)
10.1152/ajpcell.1978.234.3.C59 / Am J Physiol by Sakurada O (1978)
10.1111/j.1471-4159.1977.tb10649.x

Dates

Type	When
Created	14 years, 4 months ago (April 8, 2011, 1:27 p.m.)
Deposited	5 months, 2 weeks ago (March 10, 2025, 7:05 p.m.)
Indexed	5 months, 2 weeks ago (March 11, 2025, 12:37 a.m.)
Issued	42 years, 5 months ago (March 1, 1983)
Published	42 years, 5 months ago (March 1, 1983)
Published Online	42 years, 5 months ago (March 1, 1983)
Published Print	42 years, 5 months ago (March 1, 1983)

Funders 0

None

BibTeX

@article{Furlow_1983, title={Simultaneous Measurement of Local Glucose Utilization and Blood Flow in the Rat Brain: An Autoradiographic Method Using Two Tracers Labeled with Carbon-14}, volume={3}, ISSN={1559-7016}, url={http://dx.doi.org/10.1038/jcbfm.1983.7}, DOI={10.1038/jcbfm.1983.7}, number={1}, journal={Journal of Cerebral Blood Flow & Metabolism}, publisher={SAGE Publications}, author={Furlow, Thomas W. and Harrison, Lynn E.}, year={1983}, month=mar, pages={62–66} }

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