DOI: 10.1038/jcbfm.1983.13. Intracellular pH in the Brain following Transient Ischemia

Intracellular pH in the Brain following Transient Ischemia

10.1038/jcbfm.1983.13

Crossref journal-article

SAGE Publications

Journal of Cerebral Blood Flow & Metabolism (179)

Abstract

The objective of the present study was to discover whether or not intracellular alkalosis develops in the brain in the recovery period following transient ischemia. Forebrain ischemia of 15-min duration was induced by four-vessel occlusion in rats, with recovery periods of 15, 60, and 180 min. Intracellular pH was derived both by the HCO3−–H2CO3 method and from the creatine kinase equilibrium. The ischemia was associated with energy failure and marked accumulation of lactic acid in the cerebral cortex. Recirculation brought about rapid rephosphorylation of adenine nucleotides and gradual normalization of lactic acid levels. After 15 min of recovery, the HCO3−–H2CO3 method indicated persisting acidosis, but the creatine kinase reaction did not. After 60 min, a shift of pH in the alkaline direction was demonstrated in both methods. This alkalosis had disappeared after 3 h of recovery. It is concluded that resumption of ATP production after ischemia is followed by a rapid rise in intracellular pH, which transiently increases above normal.

Authors 3

Hideo Mabe (first)
Photjanee Blomqvist (additional)
Bo K. Siesjö (additional)

References 27 Referenced 114

10.1097/00000542-198003000-00002
10.1111/j.1471-4159.1972.tb01309.x
10.1161/01.STR.8.1.77
10.1002/ana.410080310
{'key': 'bibr5-jcbfm.1983.13', 'first-page': '515', 'volume-title': 'The Enzymes', 'volume': '6', 'author': 'Kuby SA', 'year': '1962'} / The Enzymes by Kuby SA (1962)
10.1111/j.1471-4159.1977.tb07709.x
10.1016/0006-8993(74)91050-6
10.1016/0006-8993(74)90782-3
10.1111/j.1471-4159.1973.tb07582.x
10.1111/j.1471-4159.1973.tb00283.x
10.3171/jns.1975.43.3.0318
10.1111/j.1748-1716.1970.tb04778.x
10.1161/01.STR.9.4.327
10.1111/j.1471-4159.1978.tb06553.x
10.1161/01.STR.9.4.335
10.1038/jcbfm.1981.9
10.1016/0006-8993(81)91068-4
10.1111/j.1748-1716.1966.tb03294.x
10.1111/j.1471-4159.1973.tb07567.x
10.1161/01.STR.10.3.267
10.1002/ana.410110510
10.1038/jcbfm.1981.34
10.1073/pnas.61.3.1079
10.1161/01.STR.12.2.236
{'volume-title': 'Brain Energy Metabolism', 'year': '1978', 'author': 'Siesjö BK', 'key': 'bibr25-jcbfm.1983.13'} / Brain Energy Metabolism by Siesjö BK (1978)
10.1038/jcbfm.1981.18
10.1111/j.1471-4159.1972.tb01308.x

Dates

Type	When
Created	14 years, 4 months ago (April 8, 2011, 1:27 p.m.)
Deposited	5 months, 3 weeks ago (March 10, 2025, 12:34 p.m.)
Indexed	2 days, 3 hours ago (Sept. 3, 2025, 7:10 a.m.)
Issued	42 years, 6 months ago (March 1, 1983)
Published	42 years, 6 months ago (March 1, 1983)
Published Online	42 years, 6 months ago (March 1, 1983)
Published Print	42 years, 6 months ago (March 1, 1983)

Funders 0

None

BibTeX

@article{Mabe_1983, title={Intracellular pH in the Brain following Transient Ischemia}, volume={3}, ISSN={1559-7016}, url={http://dx.doi.org/10.1038/jcbfm.1983.13}, DOI={10.1038/jcbfm.1983.13}, number={1}, journal={Journal of Cerebral Blood Flow & Metabolism}, publisher={SAGE Publications}, author={Mabe, Hideo and Blomqvist, Photjanee and Siesjö, Bo K.}, year={1983}, month=mar, pages={109–114} }

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