DOI: 10.1038/embor.2010.17. Mitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA

Mitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA

10.1038/embor.2010.17

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Although a considerable amount of data have been gathered on mitochondrial translocases, which control the import of a large number of nuclear‐encoded proteins, the preceding steps taking place in the cytosol are poorly characterized. The localization of messenger RNAs (mRNAs) on the surface of mitochondria was recently shown to involve specific classes of protein and could be an important regulatory step. By using an improved statistical fluorescent in situ hybridization technique, we analysed the elements of the ATP2 open reading frame that control its mRNA asymmetric localization. The amino‐terminal mitochondrial targeting peptide (MTS) and translation of two elements in the coding sequence, R1 and R2, were required for anchoring of ATP2 mRNA to mitochondria. Unexpectedly, any MTS can replace ATP2 MTS, whereas R1 and R2 are specifically required to maintain perimitochondrial mRNA localization. These data connect the well‐known MTS–translocase interaction step with a site‐specific translation step and offer a mechanistic description for a co‐translational import process.

Bibliography

Garcia, M., Delaveau, T., Goussard, S., & Jacq, C. (2010). Mitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA. EMBO Reports, 11(4), 285â291. Portico.

Authors 4

Mathilde Garcia (first)
Thierry Delaveau (additional)
Sebastien Goussard (additional)
Claude Jacq (additional)

References 25 Referenced 53

10.1016/S1937-6448(08)01802-9
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10.1091/mbc.E06-09-0827
GarciaM DarzacqX DevauxF SingerR JacqC(2007b)Yeast Mitochondrial Transcriptomics. Totowa New Jersey USA: Humana
{'key': 'e_1_2_6_6_1', 'first-page': '197', 'volume-title': 'J Cell Biol', 'author': 'Garcia‐Rodriguez LJ', 'year': '2007'} / J Cell Biol by Garcia‐Rodriguez LJ (2007)
10.1016/S0014-5793(02)02528-0
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10.1146/annurev.biochem.76.052705.163409
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10.1126/science.1120499

Dates

Type	When
Created	15 years, 5 months ago (March 12, 2010, 4:51 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:56 p.m.)
Indexed	4 months, 2 weeks ago (April 9, 2025, 12:48 a.m.)
Issued	15 years, 5 months ago (March 12, 2010)
Published	15 years, 5 months ago (March 12, 2010)
Published Online	15 years, 5 months ago (March 12, 2010)
Published Print	15 years, 4 months ago (April 1, 2010)

Funders 0

None

BibTeX

@article{Garcia_2010, title={Mitochondrial presequence and open reading frame mediate asymmetric localization of messenger RNA}, volume={11}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/embor.2010.17}, DOI={10.1038/embor.2010.17}, number={4}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Garcia, Mathilde and Delaveau, Thierry and Goussard, Sebastien and Jacq, Claude}, year={2010}, month=mar, pages={285–291} }

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