DOI: 10.1038/embor.2009.202. Inter‐subunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V<sub>1</sub>‐ATPase

Inter‐subunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V1‐ATPase

10.1038/embor.2009.202

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

V‐type ATPases (V‐ATPases) are categorized as rotary ATP synthase/ATPase complexes. The V‐ATPases are distinct from F‐ATPases in terms of their rotation scheme, architecture and subunit composition. However, there is no detailed structural information on V‐ATPases despite the abundant biochemical and biophysical research. Here, we report a crystallographic study of V1‐ATPase, from Thermus thermophilus, which is a soluble component consisting of A, B, D and F subunits. The structure at 4.5 Å resolution reveals inter‐subunit interactions and nucleotide binding. In particular, the structure of the central stalk composed of D and F subunits was shown to be characteristic of V1‐ATPases. Small conformational changes of respective subunits and significant rearrangement of the quaternary structure observed in the three AB pairs were related to the interaction with the straight central stalk. The rotation mechanism is discussed based on a structural comparison between V1‐ATPases and F1‐ATPases.

Bibliography

Numoto, N., Hasegawa, Y., Takeda, K., & Miki, K. (2009). Interâsubunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V1âATPase. EMBO Reports, 10(11), 1228â1234. Portico.

Authors 4

Nobutaka Numoto (first)
Yu Hasegawa (additional)
Kazuki Takeda (additional)
Kunio Miki (additional)

References 28 Referenced 53

Dates

Type	When
Created	15 years, 10 months ago (Sept. 25, 2009, 11:20 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:59 p.m.)
Indexed	1 year, 1 month ago (July 13, 2024, 7:14 p.m.)
Issued	15 years, 10 months ago (Sept. 25, 2009)
Published	15 years, 10 months ago (Sept. 25, 2009)
Published Online	15 years, 10 months ago (Sept. 25, 2009)
Published Print	15 years, 9 months ago (Nov. 1, 2009)

Funders 0

None

BibTeX

@article{Numoto_2009, title={Inter‐subunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V1‐ATPase}, volume={10}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/embor.2009.202}, DOI={10.1038/embor.2009.202}, number={11}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Numoto, Nobutaka and Hasegawa, Yu and Takeda, Kazuki and Miki, Kunio}, year={2009}, month=sep, pages={1228–1234} }

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