DOI: 10.1038/embor.2008.72. The La‐related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes

10.1038/embor.2008.72

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

The positive transcription elongation factor b (P‐TEFb) is a heterodimeric complex composed of cyclin‐dependent kinase 9 and its regulator cyclin T1/2. It stimulates transcription elongation by phosphorylation of serine 2 residues in the carboxy‐terminal domain of polymerase II. 7SK RNA and HEXIM proteins can antagonize transcriptional stimulation by sequestering P‐TEFb in a catalytically inactive ribonucleoprotein (RNP). Here, we show that the previously uncharacterized La‐related protein 7 (LARP7) has a role in 7SK‐mediated regulation of transcription. LARP7 binds to the highly conserved 3′‐terminal U‐rich stretch of 7SK RNA and is an integral part of the 7SK RNP. On stimulation, LARP7 remains associated with 7SK RNA, whereas P‐TEFb is released. Interestingly, reduction of LARP7 by RNA interference enhances transcription from cellular polymerase II promoters, as well as a TAT‐dependent HIV‐1 promoter. Thus, LARP7 is a negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system.

Bibliography

Markert, A., Grimm, M., Martinez, J., Wiesner, J., Meyerhans, A., Meyuhas, O., Sickmann, A., & Fischer, U. (2008). The Laârelated protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes. EMBO Reports, 9(6), 569â575. Portico.

Authors 8

Andreas Markert (first)
Michael Grimm (additional)
Javier Martinez (additional)
Julia Wiesner (additional)
Andreas Meyerhans (additional)
Oded Meyuhas (additional)
Albert Sickmann (additional)
Utz Fischer (additional)

References 22 Referenced 155

Dates

Type	When
Created	17 years, 3 months ago (May 16, 2008, 5:11 a.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:57 p.m.)
Indexed	1 month ago (July 28, 2025, 2:41 a.m.)
Issued	17 years, 3 months ago (May 16, 2008)
Published	17 years, 3 months ago (May 16, 2008)
Published Online	17 years, 3 months ago (May 16, 2008)
Published Print	17 years, 2 months ago (June 1, 2008)

Funders 0

None

BibTeX

@article{Markert_2008, title={The La‐related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes}, volume={9}, ISSN={1469-3178}, url={http://dx.doi.org/10.1038/embor.2008.72}, DOI={10.1038/embor.2008.72}, number={6}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Markert, Andreas and Grimm, Michael and Martinez, Javier and Wiesner, Julia and Meyerhans, Andreas and Meyuhas, Oded and Sickmann, Albert and Fischer, Utz}, year={2008}, month=may, pages={569–575} }

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