Synthetic DNA delivery systems
10.1038/71889
Crossref journal-article
Springer Science and Business Media LLC
Nature Biotechnology (297)
Bibliography

Luo, D., & Saltzman, W. M. (2000). Synthetic DNA delivery systems. Nature Biotechnology, 18(1), 33–37.

Authors 2
  1. Dan Luo (first)
  2. W. Mark Saltzman (additional)
References 80 Referenced 1,246
  1. Rolland, A.P. From genes to gene medicines: recent advances in nonviral gene delivery. Crit. Rev. Ther. Drug Carrier Syst. 15, 143– 198 (1998). (10.1615/CritRevTherDrugCarrierSyst.v15.i2.20) / Crit. Rev. Ther. Drug Carrier Syst. by AP Rolland (1998)
  2. Fasbender, A., Zabner, J., Zeiher, B.G. & Welsh, M.J. A low rate of cell proliferation and reduced DNA uptake limit cationic lipid-mediated gene transfer to primary cultures of ciliated human airway epithelia. Gene Ther. 4, 1173–1180 (1997). (10.1038/sj.gt.3300524) / Gene Ther. by A Fasbender (1997)
  3. Matsui, H., Johnson, L.G., Randell, S.H. & Boucher, R.C. Loss of binding and entry of liposome–DNA complexes decreases transfection efficiency in differentiated airway epithelial cells. J. Biol. Chem. 272, 1117–1126 ( 1997). (10.1074/jbc.272.2.1117) / J. Biol. Chem by H Matsui (1997)
  4. Anderson, W.F. Human gene therapy. Nature 392, 25– 30 (1998). (10.1038/32801) / Nature by WF Anderson (1998)
  5. Crystal, R.G. Transfer of genes to humans: early lessons and obstacles to success. Science 270, 404–410 ( 1995). (10.1126/science.270.5235.404) / Science by RG Crystal (1995)
  6. Tripathy, S.K., Black, H.B., Goldwasser, E. & Leiden, J.M. Immune responses to transgene-encoded proteins limit the stability of gene expression after injection of replication-defective adenovirus vectors. Nat. Med. 2, 545–550 ( 1996). (10.1038/nm0596-545) / Nat. Med. by SK Tripathy (1996)
  7. Mann, M.J. et al. Pressure-mediated oligonucleotide transfection of rat and human cardiovascular tissues. Proc. Natl. Acad. Sci. USA 96, 6411–6416 (1999). (10.1073/pnas.96.11.6411) / Proc. Natl. Acad. Sci. USA by MJ Mann (1999)
  8. Liu, F., Song, Y. & Liu, D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 6, 1258– 1266 (1999). (10.1038/sj.gt.3300947) / Gene Ther by F Liu (1999)
  9. Zhang, G., Budker, V. & Wolff, J.A. High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA. Hum. Gene Ther. 10, 1735–1737 ( 1999). (10.1089/10430349950017734) / Hum. Gene Ther. by G Zhang (1999)
  10. Pillai, R et al. Ultrasonic nebulization of catonic lipid-based gene delivery systems for airway administration. Pharm. Res. 15, 1743– 1747.
  11. Yang, N.S., Burkholder, J., Roberts, B., Martinell, B. & McCabe, D. In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment. Proc. Natl. Acad. Sci. USA 87, 9568–9572 (1990). (10.1073/pnas.87.24.9568) / Proc. Natl. Acad. Sci. USA by NS Yang (1990)
  12. Yang, N.S. & Sun, W.H. Gene gun and other non-viral approaches for cancer gene therapy. Nat. Med. 1, 481 –483 (1995). (10.1038/nm0595-481) / Nat. Med. by NS Yang (1995)
  13. Ye, G.N., Daniell, H. & Sanford, J.C. Optimization of delivery of foreign DNA into higher-plant chloroplasts. Plant Mol. Biol. 15, 809– 819 (1990). (10.1007/BF00039421) / Plant Mol. Biol. by GN Ye (1990)
  14. Qiu, P., Ziegelhoffer, P., Sun, J. & Yang, N.S. Gene gun delivery of mRNA in situ results in efficient transgene expression and genetic immunization. Gene Ther. 3, 262–268 (1996). / Gene Ther. by P Qiu (1996)
  15. Fynan, E.F. et al. DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc. Natl. Acad. Sci. USA 90 , 11478–11482 (1993). (10.1073/pnas.90.24.11478) / Proc. Natl. Acad. Sci. USA by EF Fynan (1993)
  16. Wong, T.K. & Neumann, E. Electric field mediated gene transfer . Biochem. Biophys. Res. Commun. 107, 584 –587 (1982). (10.1016/0006-291X(82)91531-5) / Biochem. Biophys. Res. Commun. by TK Wong (1982)
  17. Neumann, E., Schaefer-Ridder, M., Wang, Y. & Hofschneider, P.H. Gene transfer into mouse lyoma cells by electroporation in high electric fields . EMBO J. 1, 841–845 (1982). (10.1002/j.1460-2075.1982.tb01257.x) / EMBO J. by E Neumann (1982)
  18. Banga, A.K. & Prausnitz, M.R. Assessing the potential of skin electroporation for the delivery of protein- and gene-based drugs. Trends Biotechnol. 16, 408–412 (1998). (10.1016/S0167-7799(98)01238-4) / Trends Biotechnol. by AK Banga (1998)
  19. Oshima, Y. et al. Targeted gene transfer to corneal endothelium in vivo by electric pulse. Gene Ther. 5, 1347–1354 (1998). (10.1038/sj.gt.3300725) / Gene Ther. by Y Oshima (1998)
  20. Aihara, H. & Miyazaki, J. Gene transfer into muscle by electroporation in vivo. Nat. Biotechnol. 16, 867– 870 (1998). (10.1038/nbt0998-867) / Nat. Biotechnol. by H Aihara (1998)
  21. Rizzuto, G. et al. Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. Proc. Natl. Acad. Sci. USA 96, 6417–6422 (1999). (10.1073/pnas.96.11.6417) / Proc. Natl. Acad. Sci. USA by G Rizzuto (1999)
  22. Vaheri, A. & Pagano, J.S. Infectious poliovirus RNA: a sensitive method of assay. Virology 27, 434– 436 (1965). (10.1016/0042-6822(65)90126-1) / Virology by A Vaheri (1965)
  23. Felgner, P.L. Particulate systems and polymers for in vitro and in vivo delivery of polynucleotides . Adv. Drug Deliv. Rev. 5, 163– 187 (1990). (10.1016/0169-409X(90)90015-K) / Adv. Drug Deliv. Rev. by PL Felgner (1990)
  24. Graham, F.L. & Eb, A.J.V.D. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467 (1973). (10.1016/0042-6822(73)90341-3) / Virology by FL Graham (1973)
  25. Felgner, P.L. et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84, 7413–7417 (1987). (10.1073/pnas.84.21.7413) / Proc. Natl. Acad. Sci. USA by PL Felgner (1987)
  26. Lee, R.J. & Huang, L. Lipidic vector systems for gene transfer . Crit. Rev. Ther. Drug Carrier Syst. 14, 173–206 (1997). (10.1615/CritRevTherDrugCarrierSyst.v14.i2.30) / Crit. Rev. Ther. Drug Carrier Syst. by RJ Lee (1997)
  27. Templeton, N.S. & Lasic, D.D. New directions in liposome gene delivery. Mol Biotechnol 11, 175–180 (1999). (10.1007/BF02915810) / Mol Biotechnol by NS Templeton (1999)
  28. Monkkonen, J. & Urtti, A. Lipid fusion in oligonucleotide and gene delivery with cationic lipids. Adv. Drug Del. Rev. 34, 37–49 (1998). (10.1016/S0169-409X(98)00004-0) / Adv. Drug Del. Rev. by J Monkkonen (1998)
  29. Maurer, N. et al. Lipid-based systems for the intracellular delivery of genetic drugs. Mol. Membr. Biol. 16, 129–140 (1999). (10.1080/096876899294869) / Mol. Membr. Biol. by N Maurer (1999)
  30. Schuber, F., Kichler, A., Boeckler, C. & Frisch, B. Liposomes: from membrane models to gene therapy. Pure Appl. Chem. 70, 89–96 ( 1998). (10.1351/pac199870010089) / Pure Appl. Chem. by F Schuber (1998)
  31. Smith, J., Zhang, Y.L. & Niven, R. Toward development of a non-viral gene therapeutic. Adv. Drug Del. Rev. 26, 135–150 (1997). (10.1016/S0169-409X(97)00031-8) / Adv. Drug Del. Rev. by J Smith (1997)
  32. Filion, M.C. & Phillips, N.C. Major limitations in the use of cationic liposomes for DNA delivery. Int. J. Pharmaceut. 162, 159–170 (1998). (10.1016/S0378-5173(97)00423-7) / Int. J. Pharmaceut. by MC Filion (1998)
  33. Wolff, J.A. et al. Direct gene transfer into mouse muscle in vivo. Science 247, 1465–1468 ( 1990). (10.1126/science.1690918) / Science by JA Wolff (1990)
  34. Zabner, J. et al. Comparison of DNA–lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo. J. Clin. Invest. 100, 1529–1537 (1997). (10.1172/JCI119676) / J. Clin. Invest. by J Zabner (1997)
  35. Zauner, W., Ogris, M. & Wagner, E. Polylysine-based transfection systems utilizing receptor- mediated delivery. Adv. Drug Del. Rev. 30, 97–113 (1998). (10.1016/S0169-409X(97)00110-5) / Adv. Drug Del. Rev. by W Zauner (1998)
  36. Kim, J.S., Kim, B.I., Maruyama, A., Akaike, T. & Kim, S.W. A new non-viral DNA delivery vector: the terplex system . J. Contr. Release 53, 175– 182 (1998). (10.1016/S0168-3659(97)00251-4) / J. Contr. Release by JS Kim (1998)
  37. Wu, C.H., Wilson, J.M. & Wu, G.Y. Targeting genes: delivery and persistent expression of a foreign gene driven by mammalian regulatory elements in vivo. J. Biol. Chem. 264, 16985–16987 (1989). (10.1016/S0021-9258(18)71447-6) / J. Biol. Chem. by CH Wu (1989)
  38. Schaffer, D.V. & Lauffenburger, D.A. Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery. J. Biol. Chem. 273, 28004 –28009 (1998). (10.1074/jbc.273.43.28004) / J. Biol. Chem. by DV Schaffer (1998)
  39. Sorgi, F.L., Bhattacharya, S. & Huang, L. Protamine sulfate enhances lipid-mediated gene transfer . Gene Ther. 4, 961–968 (1997). (10.1038/sj.gt.3300484) / Gene Ther. by FL Sorgi (1997)
  40. Schwartz, B. et al. Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo. Gene Ther. 6, 282–292 ( 1999). (10.1038/sj.gt.3300795) / Gene Ther. by B Schwartz (1999)
  41. Paul, R.W. et al. Gene transfer using a novel fusion protein, GAL 4/invasin. Hum Gene Ther. 8, 1253–1262 (1997). (10.1089/hum.1997.8.10-1253) / Hum Gene Ther. by RW Paul (1997)
  42. Smith, L.C. et al. Synthetic peptide-based DNA complexes for nonviral gene delivery . Adv. Drug Del. Rev. 30, 115– 131 (1998). (10.1016/S0169-409X(97)00111-7) / Adv. Drug Del. Rev. by LC Smith (1998)
  43. Karak, N. & Maiti, S. Dendritic polymers: a class of novel material. J. Polymer Mater. 14, 105 ( 1997).
  44. Haensler, J. & Szoka, F.C., Jr. Polyamidoamine cascade polymers mediate efficient transfection of cells in culture. Bioconjug. Chem. 4, 372–379 (1993). (10.1021/bc00023a012) / Bioconjug. Chem. by J Haensler (1993)
  45. Kukowska-Latallo, J.F. et al. Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers. Proc. Natl. Acad. Sci. USA 93, 4897–4902 ( 1996). (10.1073/pnas.93.10.4897) / Proc. Natl. Acad. Sci. USA by JF Kukowska-Latallo (1996)
  46. Tang, M.X., Redemann, C.T. & Szoka, F.C. Jr. In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjug. Chem. 7, 703–714 (1996). (10.1021/bc9600630) / Bioconjug. Chem. by MX Tang (1996)
  47. Choi, J.S., Lee, E.J., Choi, Y.H., Jeong, Y.J. & Park, J.S. Poly(ethylene glycol)-block-poly(l-lysine) dendrimer: novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA. Bioconjug. Chem. 10, 62–65 (1999). (10.1021/bc9800668) / Bioconjug. Chem. by JS Choi (1999)
  48. Godbey, W., Wu, K., Hirasaki, G. & Mikos, A. Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency. Gene Ther. 6, 1380–1388 (1999). (10.1038/sj.gt.3300976) / Gene Ther. by W Godbey (1999)
  49. Diebold, S.S., Kursa, M., Wagner, E., Cotten, M. & Zenke, M. Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells. J. Biol. Chem. 274, 19087–19094 (1999). (10.1074/jbc.274.27.19087) / J. Biol. Chem. by SS Diebold (1999)
  50. Ogris, M. et al. The size of DNA/transferrin–PEI complexes is an important factor for gene expression in cultured cells. Gene Ther. 5, 1425–1433 (1998). (10.1038/sj.gt.3300745) / Gene Ther. by M Ogris (1998)
  51. Chemin, I. et al. Liver-directed gene transfer: a linear polyethlenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo . J. Viral Hepat. 5, 369– 375 (1998). (10.1046/j.1365-2893.1998.00126.x) / J. Viral Hepat. by I Chemin (1998)
  52. Truong-Le, V.L., August, J.T. & Leong, K.W. Controlled gene delivery by DNA-gelatin nanospheres . Hum. Gene Ther. 9, 1709– 1717 (1998). (10.1089/hum.1998.9.12-1709) / Hum. Gene Ther. by VL Truong-Le (1998)
  53. Leong, K.W. et al. DNA–polycation nanospheres as nonviral gene delivery vehicles . J. Contr. Release 53, 183– 193 (1998). (10.1016/S0168-3659(97)00252-6) / J. Contr. Release by KW Leong (1998)
  54. van de Wetering, P., Moret, E.E., Schuurmans-Nieuwenbroek, N.M., van Steenbergen, M.J. & Hennink, W.E. Structure–activity relationships of water-soluble cationic methacrylate/methacrylamide polymers for nonviral gene delivery. Bioconjug. Chem. 10, 589–597 (1999). (10.1021/bc980148w) / Bioconjug. Chem. by P van de Wetering (1999)
  55. Van de Woude, I. et al. Novel pyridinium surfactants for efficient, nontoxic in vitro gene delivery. Proc. Natl. Acad. Sci. USA 94, 1160–1165 (1997). (10.1073/pnas.94.4.1160) / Proc. Natl. Acad. Sci. USA by I Van de Woude (1997)
  56. Jones, D.H., Corris, S., McDonald, S., Clegg, J.C. & Farrar, G.H. Poly(dl-lactide-co-glycolide)-encapsulated plasmid DNA elicits systemic and mucosal antibody responses to encoded protein after oral administration. Vaccine 15, 814 –817 (1997). (10.1016/S0264-410X(96)00266-6) / Vaccine by DH Jones (1997)
  57. Ando, S., Putnam, D., Pack, D.W. & Langer, R. PLGA microspheres containing plasmid DNA: preservation of supercoiled DNA via cryopreparation and carbohydrate stabilization. J. Pharm. Sci. 88, 126–130 (1999). (10.1021/js9801687) / J. Pharm. Sci. by S Ando (1999)
  58. Wang, D., Robinson, D.R., Kwon, G.S. & Samuel, J. Encapsulation of plasmid DNA in biodegradable poly(d,l-lactic- co-glycolic acid) microspheres as a novel approach for immunogene delivery . J. Contr. Release 57, 9– 18 (1999). (10.1016/S0168-3659(98)00099-6) / J. Contr. Release by D Wang (1999)
  59. Luo, D., Woodrow-Mumford, K., Belcheva, N. & Saltzman, W.M. Controlled DNA delivery systems. Pharm. Res. 16, 1300–1308 (1999). (10.1023/A:1014870102295) / Pharm. Res. by D Luo (1999)
  60. Shea, L.D., Smiley, E., Bonadio, J. & Mooney, D.J. DNA delivery from polymer matrices for tissue engineering. Nat. Biotechnol. 17, 551–554 ( 1999). (10.1038/9853) / Nat. Biotechnol. by LD Shea (1999)
  61. Pouton, C.W. & Seymour, L.W. Key issues in non-viral gene delivery . Adv. Drug Del. Rev. 34, 3– 19 (1998). (10.1016/S0169-409X(98)00048-9) / Adv. Drug Del. Rev. by CW Pouton (1998)
  62. El Ouahabi, A. et al. The role of endosome destabilizing activity in the gene transfer process mediated by cationic lipids. FEBS Lett. 414 , 187–192 (1997). (10.1016/S0014-5793(97)00973-3) / FEBS Lett. by A El Ouahabi (1997)
  63. Wagner, E. et al. Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc. Natl. Acad. Sci. USA 89, 6099– 6103 (1992). (10.1073/pnas.89.13.6099) / Proc. Natl. Acad. Sci. USA by E Wagner (1992)
  64. Boussif, O. et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. USA 92, 7297–7301 ( 1995). (10.1073/pnas.92.16.7297) / Proc. Natl. Acad. Sci. USA by O Boussif (1995)
  65. Duguid, J.G. et al. A physicochemical approach for predicting the effectiveness of peptide-based gene delivery systems for use in plasmid-based gene therapy. Biophys. J. 74, 2802–2814 ( 1998). (10.1016/S0006-3495(98)77987-1) / Biophys. J. by JG Duguid (1998)
  66. Uherek, C., Fominaya, J. & Wels, W. A modular DNA carrier protein based on the structure of diphtheria toxin mediates target cell-specific gene delivery. J. Biol. Chem. 273, 8835–8841 (1998). (10.1074/jbc.273.15.8835) / J. Biol. Chem. by C Uherek (1998)
  67. Greber, U.F., Willetts, M., Webster, P. & Helenius, A. Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75, 477–486 ( 1993). (10.1016/0092-8674(93)90382-Z) / Cell by UF Greber (1993)
  68. Wagner, E., Plank, C., Zatloukal, K., Cotten, M. & Birnstiel, M.L. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle. Proc. Natl. Acad. Sci. USA 89, 7934–7938 ( 1992). (10.1073/pnas.89.17.7934) / Proc. Natl. Acad. Sci. USA by E Wagner (1992)
  69. Plank, C., Oberhauser, B., Mechtler, K., Koch, C. & Wagner, E. The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems . J. Biol. Chem. 269, 12918– 12924 (1994). (10.1016/S0021-9258(18)99963-1) / J. Biol. Chem. by C Plank (1994)
  70. Katayose, S. & Kataoka, K. Water-soluble polyion complex associates of DNA and poly(ethylene glycol)-poly(l-lysine) block copolymer. Bioconjug. Chem. 8, 702–707 (1997). (10.1021/bc9701306) / Bioconjug. Chem. by S Katayose (1997)
  71. Xu, B., Wiehle, S., Roth, J.A. & Cristiano, R.J. The contribution of poly-l-lysine, epidermal growth factor and streptavidin to EGF/PLL/DNA polyplex formation. Gene Ther. 5, 1235– 1243 (1998). (10.1038/sj.gt.3300719) / Gene Ther. by B Xu (1998)
  72. Ross, G.F. et al. Enhanced reporter gene expression in cells transfected in the presence of DMI-2, an acid nuclease inhibitor. Gene Ther. 5, 1244–1250 (1998). (10.1038/sj.gt.3300721) / Gene Ther. by GF Ross (1998)
  73. Schaffer, D.V., Fidelman, N.A., Dan, N. & Lauffenburger, D.A. Vector unpackaging as a potential barrier for receptor-mediated polyplex gene delivery . Biotechnol. Bioeng., in press (1999). (10.1002/(SICI)1097-0290(20000305)67:5<598::AID-BIT10>3.0.CO;2-G)
  74. Pollard, H. et al. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J. Biol. Chem. 273 , 7507–7511 (1998). (10.1074/jbc.273.13.7507) / J. Biol. Chem. by H Pollard (1998)
  75. Godbey, W.T., Wu, K.K. & Mikos, A.G. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. USA 96, 5177–5181 (1999). (10.1073/pnas.96.9.5177) / Proc. Natl. Acad. Sci. USA by WT Godbey (1999)
  76. Holmes, A.R., Dohrman, A.F., Ellison, A.R., Goncz, K.K., & Gruenert, D.C. Intracellular compartmentalization of DNA fragments in cultured airway epithelial cells mediated by cationic lipids. Pharm. Res. 16, 1020– 1025 (1999). (10.1023/A:1018927531003) / Pharm. Res. by AR Holmes (1999)
  77. Branden, L.J., Mohamed, A.J. & Smith, C.I. A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA. Nat. Biotechnol. 17, 784–787 ( 1999). (10.1038/11726) / Nat. Biotechnol. by LJ Branden (1999)
  78. Ziemienowicz, A., Gorlich, D., Lanka, E., Hohn, B. & Rossi, L. Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium. Proc. Natl. Acad. Sci. USA 96, 3729–3733 (1999). (10.1073/pnas.96.7.3729) / Proc. Natl. Acad. Sci. USA by A Ziemienowicz (1999)
  79. Subramanian, A., Ranganathan, P. & Diamond, S.L. Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells. Nat. Biotechnol. 17 , 873–877 (1999). (10.1038/12860) / Nat. Biotechnol. by A Subramanian (1999)
  80. Mahato, R.I., Takakura, Y. & Hashida, M. Nonviral vectors for in vivo gene delivery: physicochemical and pharmacokinetic considerations. Crit. Rev. Ther. Drug Carrier Syst. 14, 133–172 ( 1997). (10.1615/CritRevTherDrugCarrierSyst.v14.i2.20) / Crit. Rev. Ther. Drug Carrier Syst. by RI Mahato (1997)
Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 4:48 a.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 3:17 p.m.)
Indexed 1 week, 4 days ago (Aug. 24, 2025, 7:02 p.m.)
Issued 25 years, 8 months ago (Jan. 1, 2000)
Published 25 years, 8 months ago (Jan. 1, 2000)
Published Print 25 years, 8 months ago (Jan. 1, 2000)
Funders 0

None

@article{Luo_2000, title={Synthetic DNA delivery systems}, volume={18}, ISSN={1546-1696}, url={http://dx.doi.org/10.1038/71889}, DOI={10.1038/71889}, number={1}, journal={Nature Biotechnology}, publisher={Springer Science and Business Media LLC}, author={Luo, Dan and Saltzman, W. Mark}, year={2000}, month=jan, pages={33–37} }