DOI: 10.1038/46558. Large-scale analysis of the yeast genome by transposon tagging and gene disruption

Large-scale analysis of the yeast genome by transposon tagging and gene disruption

10.1038/46558

Crossref journal-article

Springer Science and Business Media LLC

Nature (297)

Bibliography

Ross-Macdonald, P., Coelho, P. S. R., Roemer, T., Agarwal, S., Kumar, A., Jansen, R., Cheung, K.-H., Sheehan, A., Symoniatis, D., Umansky, L., Heidtman, M., Nelson, F. K., Iwasaki, H., Hager, K., Gerstein, M., Miller, P., Roeder, G. S., & Snyder, M. (1999). Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature, 402(6760), 413â418.

Authors 18

Petra Ross-Macdonald (first)
Paulo S. R. Coelho (additional)
Terry Roemer (additional)
Seema Agarwal (additional)
Anuj Kumar (additional)
Ronald Jansen (additional)
Kei-Hoi Cheung (additional)
Amy Sheehan (additional)
Dawn Symoniatis (additional)
Lara Umansky (additional)
Matthew Heidtman (additional)
F. Kenneth Nelson (additional)
Hiroshi Iwasaki (additional)
Karl Hager (additional)
Mark Gerstein (additional)
Perry Miller (additional)
G. Shirleen Roeder (additional)
Michael Snyder (additional)

References 30 Referenced 397

Goffeau,A. et al. Life with 6000 genes. Science 274, 546–567 (1996). (10.1126/science.274.5287.546) / Science by A Goffeau (1996)
Mewes,H. W. et al. Overview of the yeast genome. Nature 387, 7–65 (1997). (10.1038/42755) / Nature by HW Mewes (1997)
Hieter,P. & Boguski,M. Functional genomics: it's all how you read it. Science 278, 601–602 (1997). (10.1126/science.278.5338.601) / Science by P Hieter (1997)
Velculescu,V. E. et al. Characterization of the yeast transcriptome. Cell 88, 243–251 (1997). (10.1016/S0092-8674(00)81845-0) / Cell by VE Velculescu (1997)
DeRisi,J. L., Iyer,V. R. & Brown,P. O. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278, 680–686 (1997). (10.1126/science.278.5338.680) / Science by JL DeRisi (1997)
Shoemaker,D. D., Lashkari,D. A., Morris,D., Mittmann,M. & Davis,R. W. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy. Nature Genet. 14, 450–456 (1996). (10.1038/ng1296-450) / Nature Genet. by DD Shoemaker (1996)
Smith,V., Chou,K. N., Lashkari,D., Botstein,D. & Brown,P. O. Functional analysis of the genes of yeast chromosome V by genetic footprinting. Science 274, 2069–2074 (1996). (10.1126/science.274.5295.2069) / Science by V Smith (1996)
Burns,N. et al. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8, 1087–1105 (1994). (10.1101/gad.8.9.1087) / Genes Dev. by N Burns (1994)
Ross-Macdonald,P.-R., Sheehan,A., Roeder,G. S. & Snyder,M. A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA 94, 190–195 (1997). (10.1073/pnas.94.1.190) / Proc. Natl Acad. Sci. USA by P-R Ross-Macdonald (1997)
Ross-Macdonald,P. et al. Methods for large-scale analysis of gene expression, protein localization, and disruption phenotypes in Saccharomyces cerevisiae. Methods Mol. Cell. Biol. 5, 298–308 (1995). / Methods Mol. Cell. Biol. by P Ross-Macdonald (1995)
Cherry,M. et al. SGD: Saccharomyces genome database. Nucleic Acids Res. 26, 73–79 (1998). (10.1093/nar/26.1.73) / Nucleic Acids Res. by M Cherry (1998)
Schatz,P. J., Pillus,L., Grisafi,P., Solomon,F. & Botstein,D. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol. Cell. Biol. 6, 3711–3721 (1986). (10.1128/MCB.6.11.3711) / Mol. Cell. Biol. by PJ Schatz (1986)
Page,B. D. & Snyder,M. CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev. 6, 1414–1429 (1992). (10.1101/gad.6.8.1414) / Genes Dev. by BD Page (1992)
Stearns,T., Hoyt,M. A. & Botstein,D. Yeast mutants sensitive to antimicrotubule drugs define three genes that affect microtubule function. Genetics 124, 251–262 (1990). (10.1093/genetics/124.2.251) / Genetics by T Stearns (1990)
Evangelista,C. C. Jr, Rodriguez,T. A. M., Limbach,M. P. & Zitomer,R. S. Rox3 and Rts1 function in the global stress response pathway in baker's yeast. Genetics 142, 1083–1093 (1996). (10.1093/genetics/142.4.1083) / Genetics by CC Evangelista Jr (1996)
White,W. H. & Johnson,D. I. Characterization of synthetic-lethal mutants reveals a role for the Saccharomyces cerevisiae guanine-nucleotide exchange factor Cdc24p in vacuole function and Na+ tolerance. Genetics 147, 43–55 (1997). (10.1093/genetics/147.1.43) / Genetics by WH White (1997)
Lussier,M. et al. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147, 435–450 (1997). (10.1093/genetics/147.2.435) / Genetics by M Lussier (1997)
Lodi,T., Goffrini,P., Ferrero,I. & Donnini,C. IMP2, a gene involved in the expression of glucose-repressible genes in Saccharomyces cerevisiae. Microbiology 141, 2201–2209 (1995). (10.1099/13500872-141-9-2201) / Microbiology by T Lodi (1995)
Christman,M. F., Dietrich,F. S. & Fink,G. R. Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. Cell 55, 413–425 (1988). (10.1016/0092-8674(88)90027-X) / Cell by MF Christman (1988)
Shore,D. & Nasmyth,K. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements. Cell 51, 721–732 (1987). (10.1016/0092-8674(87)90095-X) / Cell by D Shore (1987)
Chu,S. et al. The transcriptional program of sporulation in budding yeast. Science 282, 699–705 (1998). (10.1126/science.282.5389.699) / Science by S Chu (1998)
Engebrecht,J. & Roeder,G. S. Mer1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis. Mol. Cell. Biol. 10, 2379–2389 (1990). (10.1128/MCB.10.5.2379) / Mol. Cell. Biol. by J Engebrecht (1990)
Sambrook,J., Fritsch,E. F. & Maniatis,T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Press, Cold Spring Harbor, New York, 1989). / Molecular Cloning: A Laboratory Manual by J Sambrook (1989)
Altschul,S. F., Gish,W., Miller,W., Meyers,E. W. & Lipman,D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403–410 (1990). (10.1016/S0022-2836(05)80360-2) / J. Mol. Biol. by SF Altschul (1990)
Kaufman,L. & Rousseeuw,P. J. Finding Groups in Data (Wiley, New York, 1990). (10.1002/9780470316801) / Finding Groups in Data by L Kaufman (1990)
Felsenstein,J. PHYLIP—phylogeny inference package (version 3.2). Cladistics 5, 164–166 (1989). / Cladistics by J Felsenstein (1989)
Fitch,W. M. & Margoliash,E. Construction of phylogenetic trees. Science 155, 279–284 (1967). (10.1126/science.155.3760.279) / Science by WM Fitch (1967)
Adams,A., Gottschling,D. E., Kaiser,C. A. & Stearns,T. Methods in Yeast Genetics (Cold Spring Harbor Press, Cold Spring Harbor, New York, 1997). / Methods in Yeast Genetics by A Adams (1997)
Goode,B. L. et al. Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast. J. Cell Biol. 144, 83–89 (1999). (10.1083/jcb.144.1.83) / J. Cell Biol. by BL Goode (1999)
Lee,W. C., Xue,Z. X. & Melese,T. The NSR1 gene encodes a protein that specifically binds nuclear localization sequences and has two RNA recognition motifs. J. Cell Biol. 113, 1–12 (1991). (10.1083/jcb.113.1.1) / J. Cell Biol. by WC Lee (1991)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 4:33 a.m.)
Deposited	2 years, 3 months ago (May 17, 2023, 8:26 p.m.)
Indexed	4 weeks, 2 days ago (July 28, 2025, 2:18 a.m.)
Issued	25 years, 9 months ago (Nov. 1, 1999)
Published	25 years, 9 months ago (Nov. 1, 1999)
Published Print	25 years, 9 months ago (Nov. 1, 1999)

Funders 0

None

BibTeX

@article{Ross_Macdonald_1999, title={Large-scale analysis of the yeast genome by transposon tagging and gene disruption}, volume={402}, ISSN={1476-4687}, url={http://dx.doi.org/10.1038/46558}, DOI={10.1038/46558}, number={6760}, journal={Nature}, publisher={Springer Science and Business Media LLC}, author={Ross-Macdonald, Petra and Coelho, Paulo S. R. and Roemer, Terry and Agarwal, Seema and Kumar, Anuj and Jansen, Ronald and Cheung, Kei-Hoi and Sheehan, Amy and Symoniatis, Dawn and Umansky, Lara and Heidtman, Matthew and Nelson, F. Kenneth and Iwasaki, Hiroshi and Hager, Karl and Gerstein, Mark and Miller, Perry and Roeder, G. Shirleen and Snyder, Michael}, year={1999}, month=nov, pages={413–418} }

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