Visualization of gene activity in living cells
10.1038/35046510
Crossref journal-article
Springer Science and Business Media LLC
Nature Cell Biology (297)
Bibliography

Tsukamoto, T., Hashiguchi, N., Janicki, S. M., Tumbar, T., Belmont, A. S., & Spector, D. L. (2000). Visualization of gene activity in living cells. Nature Cell Biology, 2(12), 871–878.

Authors 6
  1. Toshiro Tsukamoto (first)
  2. Noriyo Hashiguchi (additional)
  3. Susan M. Janicki (additional)
  4. Tudorita Tumbar (additional)
  5. Andrew S. Belmont (additional)
  6. David L. Spector (additional)
References 43 Referenced 251
  1. Spector, D. L. Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9, 265–315 ( 1993). (10.1146/annurev.cb.09.110193.001405) / Annu. Rev. Cell Biol. by DL Spector (1993)
  2. Lamond, A. I. & Earnshaw, W. C. Structure and function in the nucleus. Science 280, 547– 553 (1998). (10.1126/science.280.5363.547) / Science by AI Lamond (1998)
  3. Misteli, T. & Spector, D. L. Applications of the green fluorescent protein in cell biology and biotechnology. Nature Biotechnol. 15, 961–964 (1997). (10.1038/nbt1097-961) / Nature Biotechnol. by T Misteli (1997)
  4. Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509–544 (1998). (10.1146/annurev.biochem.67.1.509) / Annu. Rev. Biochem. by RY Tsien (1998)
  5. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J. F. Dual-colour imaging with GFP variants . Trends Cell. Biol. 9, 52– 56 (1999). (10.1016/S0962-8924(98)01420-2) / Trends Cell. Biol. by J Ellenberg (1999)
  6. Belmont, A. S. & Straight, A.F. In vivo visualization of chromosomes using lac operator–repressor binding. Trends Cell. Biol. 8, 121–124 ( 1998). (10.1016/S0962-8924(97)01211-7) / Trends Cell. Biol. by AS Belmont (1998)
  7. Robinett, C. C. et al. In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition . J. Cell Biol. 135, 1685– 1700 (1996). (10.1083/jcb.135.6.1685) / J. Cell Biol. by CC Robinett (1996)
  8. Li, G., Sudlow, G. & Belmont, A. S. Interphase cell cycle dynamics of a late-replicating, heterochromatic homogeneously staining region: precise choreography of condensation/decondensation and nuclear positioning. J. Cell Biol. 140, 975–989 (1998). (10.1083/jcb.140.5.975) / J. Cell Biol. by G Li (1998)
  9. Tumbar, T., Sudlow, G. & Belmont, A. S. Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. J. Cell Biol. 145 , 1341–1354 (1999). (10.1083/jcb.145.7.1341) / J. Cell Biol. by T Tumbar (1999)
  10. Straight, A. F., Belmont, A. S., Robinett, C. C. & Murray, A. W. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion. Curr. Biol. 6, 1599–1608 (1996). (10.1016/S0960-9822(02)70783-5) / Curr. Biol. by AF Straight (1996)
  11. Minshull, J. et al. Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast. Curr. Biol. 6, 1609–1620 (1996). (10.1016/S0960-9822(02)70784-7) / Curr. Biol. by J Minshull (1996)
  12. Miyazawa, S. et al. Peroxisome targeting signal of rat liver acyl-coenzyme A oxidase resides at the carboxy terminus. Mol. Cell. Biol. 9 , 83–91 (1989). (10.1128/MCB.9.1.83) / Mol. Cell. Biol. by S Miyazawa (1989)
  13. Miller, J. H. & Reznikoff, W. A. The Operon, 17–220 (Cold Spring Harbor Laboratory Press, New York, 1980). / The Operon by JH Miller (1980)
  14. Maul, G. G., Negorev, D., Bell, P. & Ishov, A. M. Properties and assembly mechanisms of ND10, PML bodies, or PODs. J. Struct. Biol. 129, 278–287 ( 2000). (10.1006/jsbi.2000.4239) / J. Struct. Biol. by GG Maul (2000)
  15. Zhong, S., Salomoni, P. & Pandolfi, P. P. The transcriptional role of PML and the nuclear body . Nature Cell Biol. 2, E85– E90 (2000). (10.1038/35010583) / Nature Cell Biol. by S Zhong (2000)
  16. Weintraub, H. & Groudine, M. Chromosomal subunits in active genes have an altered conformation. Science 193, 848–856 (1976). (10.1126/science.948749) / Science by H Weintraub (1976)
  17. Paranjape, S. M., Kamakaka, R. T. & Kadonaga, J. T. Role of chromatin structure in the regulation of transcription by RNA polymerase II. Annu. Rev. Biochem. 63, 265–297 (1994). (10.1146/annurev.bi.63.070194.001405) / Annu. Rev. Biochem. by SM Paranjape (1994)
  18. Armstrong, J. A. & Emerson, B. M. Transcription of chromatin: these are complex times. Curr. Opin. Genet. Dev. 8, 165–172 ( 1998). (10.1016/S0959-437X(98)80137-8) / Curr. Opin. Genet. Dev. by JA Armstrong (1998)
  19. Kuo, M. H. & Allis, C. D. Roles of histone acetyltransferases and deacetylases in gene regulation. Bioessays 20, 615–626 (1998). (10.1002/(SICI)1521-1878(199808)20:8<615::AID-BIES4>3.0.CO;2-H) / Bioessays by MH Kuo (1998)
  20. Pederson, T. Chromatin structure and gene transcription: nucleosomes permit a new synthesis . Int. Rev. Cytol. 55, 1– 21 (1978). (10.1016/S0074-7696(08)61884-7) / Int. Rev. Cytol. by T Pederson (1978)
  21. Manuelidis, L. Different central nervous system cell types display distinct and nonrandom arrangements of satellite DNA sequences. Proc. Natl Acad. Sci. USA 81, 3123–3127 ( 1984). (10.1073/pnas.81.10.3123) / Proc. Natl Acad. Sci. USA by L Manuelidis (1984)
  22. Manuelidis, L. & Borden, J. Reproducible compartmentalization of individual chromosome domains in human CNS cells revealed by in situ hybridization and three-dimensional reconstruction. Chromosoma 96 , 397–410 (1988). (10.1007/BF00303033) / Chromosoma by L Manuelidis (1988)
  23. Ferguson, M. & Ward, D. C. Cell cycle dependent chromosomal movement in pre-mitotic human T-lymphocyte nuclei. Chromosoma 101, 557–565 (1992). (10.1007/BF00660315) / Chromosoma by M Ferguson (1992)
  24. Dietzel, S. et al. Three-dimensional distribution of centromeric or paracentromeric heterochromatin of chromosomes 1, 7, 15, and 17 in human lymphocyte nuclei studied with light microscopic axial tomography. Bioimaging 3, 121–133 (1995). (10.1002/1361-6374(199509)3:3<121::AID-BIO3>3.3.CO;2-5) / Bioimaging by S Dietzel (1995)
  25. Abney, J. R., Cutler, B., Fillbach, M. L., Axelrod, D. & Scalettar, B. A. Chromatin dynamics in interphase nuclei and its implications for nuclear structure. J. Cell Biol. 137, 1459–1468 (1997). (10.1083/jcb.137.7.1459) / J. Cell Biol. by JR Abney (1997)
  26. Marshall, W. F. et al. Interphase chromosomes undergo constrained diffusional motion in living cells. Curr. Biol. 7, 930– 939 (1997). (10.1016/S0960-9822(06)00412-X) / Curr. Biol. by WF Marshall (1997)
  27. Manuelidis, L. Individual interphase chromosome domains revealed by in-situ hybridization . Hum. Genet. 71, 288–293 (1985). (10.1007/BF00388453) / Hum. Genet. by L Manuelidis (1985)
  28. Janevski, J., Park, P. C. & De Boni, U. Organization of centromeric domains in hepatocyte nuclei: rearrangement associated with de novo activation of the vitellogenin gene family in Xenopus laevis. Exp. Cell Res. 217 , 227–239 (1995). (10.1006/excr.1995.1082) / Exp. Cell Res. by J Janevski (1995)
  29. Bartholdi, M. F. Nuclear distribution of centromeres during the cell cycle of human diploid fibroblasts. J. Cell Sci. 99, 255– 263 (1991). (10.1242/jcs.99.2.255) / J. Cell Sci. by MF Bartholdi (1991)
  30. Funabiki, H., Hagan, I., Uzawa, S. & Yanagida, M. Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. J. Cell Biol. 121, 961– 976 (1993). (10.1083/jcb.121.5.961) / J. Cell Biol. by H Funabiki (1993)
  31. LaSalle, J. M. & Lalande, M. Homologous association of oppositely imprinted chromosomal domains. Science 272, 725–728 (1996). (10.1126/science.272.5262.725) / Science by JM LaSalle (1996)
  32. Borden, J. & Manuelidis, L. Movement of the X chromosome in epilepsy. Science 242, 1687 –1691 (1988). (10.1126/science.3201257) / Science by J Borden (1988)
  33. Guldner, H. H., Szostecki, C., Grotzinger, T. & Will, H. IFN enhance expression of Sp100, an autoantigen in primary biliary cirrhosis . J. Immunol. 149, 4067– 4073 (1992). (10.4049/jimmunol.149.12.4067) / J. Immunol. by HH Guldner (1992)
  34. Koken, M. H. et al. The t(15;17) translocation alters a nuclear body in a retinoic acid-reversible fashion. EMBO J. 13, 1073 –1083 (1994). (10.1002/j.1460-2075.1994.tb06356.x) / EMBO J. by MH Koken (1994)
  35. Korioth, F., Gieffers, C., Maul, G. G. & Frey, J. Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment. J. Cell Biol. 130, 1–13 (1995). (10.1083/jcb.130.1.1) / J. Cell Biol. by F Korioth (1995)
  36. Maul, G. G., Yu, E., Ishov, A. M. & Epstein, A. L. Nuclear domain 10 (ND10) associated proteins are also present in nuclear bodies and redistribute to hundreds of nuclear sites after stress. J. Cell. Biochem. 59, 498–513 (1995). (10.1002/jcb.240590410) / J. Cell. Biochem. by GG Maul (1995)
  37. Ishov, A. M. & Maul, G. G. The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition. J. Cell Biol. 134, 815–826 ( 1996). (10.1083/jcb.134.4.815) / J. Cell Biol. by AM Ishov (1996)
  38. Maul, G. G., Jensen, D. E., Ishov, A. M., Herlyn, M. & Rauscher, F. J. III Nuclear redistribution of BRCA1 during viral infection. Cell Growth Differ. 9 , 743–755 (1998). / Cell Growth Differ. by GG Maul (1998)
  39. Gongora, C. et al. Molecular cloning of a new interferon-induced PML nuclear body-associated protein. J. Biol. Chem. 272, 19457–19463 (1997). (10.1074/jbc.272.31.19457) / J. Biol. Chem. by C Gongora (1997)
  40. Chen, C. & Okayama, H. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745–2752 (1987). (10.1128/MCB.7.8.2745) / Mol. Cell. Biol. by C Chen (1987)
  41. Mintz, P. J., Patterson, S. D., Neuwald, A. F., Spahr, C. S. & Spector, D. L. Purification and biochemical characterization of interchromatin granule clusters. EMBO J. 18, 4308–4320 ( 1999). (10.1093/emboj/18.15.4308) / EMBO J. by PJ Mintz (1999)
  42. Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning (Cold Spring Harbor Laboratory Press, New York, 1989). / Molecular Cloning by J Sambrook (1989)
  43. Spector, D. L., Goldman, R. D. & Leinwand, L. A. Cells: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1998). / Cells: A Laboratory Manual by DL Spector (1998)
Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 4:47 a.m.)
Deposited 2 years, 3 months ago (May 18, 2023, 3:24 p.m.)
Indexed 1 month ago (July 28, 2025, 5:19 p.m.)
Issued 24 years, 9 months ago (Nov. 10, 2000)
Published 24 years, 9 months ago (Nov. 10, 2000)
Published Online 24 years, 9 months ago (Nov. 10, 2000)
Published Print 24 years, 9 months ago (Dec. 1, 2000)
Funders 0

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@article{Tsukamoto_2000, title={Visualization of gene activity in living cells}, volume={2}, ISSN={1476-4679}, url={http://dx.doi.org/10.1038/35046510}, DOI={10.1038/35046510}, number={12}, journal={Nature Cell Biology}, publisher={Springer Science and Business Media LLC}, author={Tsukamoto, Toshiro and Hashiguchi, Noriyo and Janicki, Susan M. and Tumbar, Tudorita and Belmont, Andrew S. and Spector, David L.}, year={2000}, month=nov, pages={871–878} }