DOI: 10.1034/j.1600-0854.2003.00072.x. Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway

Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway

10.1034/j.1600-0854.2003.00072.x

Crossref journal-article

Wiley

Traffic (311)

Abstract

We employed our recently developed immuno‐electron microscopic method (W. Möbius, Y. Ohno‐Iwashita, E. G. van Donselaar, V. M. Oorschot, Y. Shimada, T. Fujimoto, H. F. Heijnen, H. J. Geuze and J. W. Slot, J Histochem Cytochem 2002; 50: 43–55) to analyze the distribution of cholesterol in the endocytic pathway of human B lymphocytes. We could distinguish 6 categories of endocytic compartments on the basis of morphology, BSA gold uptake kinetics and organelle marker analysis. Of all cholesterol detected in the endocytic pathway, we found 20% in the recycling tubulo‐vesicles and 63% present in two types of multivesicular bodies. In the multivesicular bodies, most of the cholesterol was contained in the internal membrane vesicles, the precursors of exosomes secreted by B cells. Cholesterol was almost absent from lysosomes, that contained the bulk of the lipid bis(monoacylglycero)phosphate, also termed lysobisphosphatidic acid. Thus, cholesterol displays a highly differential distribution in the various membrane domains of the endocytic pathway.

Bibliography

MÃ¶bius, W., Van Donselaar, E., OhnoâIwashita, Y., Shimada, Y., Heijnen, H. F. G., Slot, J. W., & Geuze, H. J. (2003). Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway. Traffic, 4(4), 222â231. Portico.

Authors 7

W. Möbius (first)
E. Van Donselaar (additional)
Y. Ohno‐Iwashita (additional)
Y. Shimada (additional)
H. F. G. Heijnen (additional)
J. W. Slot (additional)
H. J. Geuze (additional)

References 77 Referenced 372

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Dates

Type	When
Created	16 years ago (Aug. 10, 2009, 11:19 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 4:38 a.m.)
Indexed	5 days, 18 hours ago (Aug. 31, 2025, 7:13 p.m.)
Issued	22 years, 5 months ago (April 1, 2003)
Published	22 years, 5 months ago (April 1, 2003)
Published Online	22 years, 4 months ago (April 9, 2003)
Published Print	22 years, 5 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{M_bius_2003, title={Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic Pathway}, volume={4}, ISSN={1600-0854}, url={http://dx.doi.org/10.1034/j.1600-0854.2003.00072.x}, DOI={10.1034/j.1600-0854.2003.00072.x}, number={4}, journal={Traffic}, publisher={Wiley}, author={Möbius, W. and Van Donselaar, E. and Ohno‐Iwashita, Y. and Shimada, Y. and Heijnen, H. F. G. and Slot, J. W. and Geuze, H. J.}, year={2003}, month=apr, pages={222–231} }

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