DOI: 10.1034/j.1600-0854.2002.31206.x. <i>Drosophila</i> Sec10 is Required for Hormone Secretion but not General Exocytosis or Neurotransmission

Drosophila Sec10 is Required for Hormone Secretion but not General Exocytosis or Neurotransmission

10.1034/j.1600-0854.2002.31206.x

Crossref journal-article

Wiley

Traffic (311)

Abstract

The sec6/8, or exocyst, complex is implicated in trafficking of secretory vesicles to fusion sites in the plasma membrane. Genetic analyses have been done primarily in yeast, where mutation of the eight protein subunits similarly disrupts polarized vesicle fusion. The goal of this study was to assay the sec6/8 complex in Drosophila, and specifically to test its widely hypothesized functions in synaptogenesis and neurotransmission. We used a transgenic RNAi approach to remove the most highly conserved complex component, Drosophila sec10 (dSec10). Ubiquitous dSec10 RNAi resulted in early postembryonic lethality, demonstrating that dSec10 is essential. Surprisingly, tissue‐specific dSec10 RNAi revealed no essential requirement in nervous system, musculature, gut or epidermis. Assays of polarized secretion in all these tissues failed to reveal any role for dSec10. In particular, the neuromuscular synapse showed no defects in morphogenesis or vesicle trafficking/fusion underlying neurotransmission. The essential requirement for dSec10 was restricted to the ring gland, the Drosophila organ specialized for endocrine function. The developmental arrest of dSec10 RNAi animals was partially rescued by feeding ecdysone, suggesting dSec10 mediates steroid hormone secretion. We conclude that dSec10 has no detectable role in most forms of polarized trafficking/exocytosis, including neurotransmission, but rather is essential for endocrine secretion.

Bibliography

Andrews, H. K., Zhang, Y. Q., Trotta, N., & Broadie, K. (2002). Drosophila Sec10 is Required for Hormone Secretion but not General Exocytosis or Neurotransmission. Traffic, 3(12), 906â921. Portico.

Authors 4

Hillary K. Andrews (first)
Yong Q. Zhang (additional)
Nick Trotta (additional)
Kendal Broadie (additional)

References 62 Referenced 45

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5:13 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 12:40 p.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 1:45 p.m.)
Issued	22 years, 9 months ago (Dec. 1, 2002)
Published	22 years, 9 months ago (Dec. 1, 2002)
Published Online	22 years, 8 months ago (Dec. 9, 2002)
Published Print	22 years, 9 months ago (Dec. 1, 2002)

Funders 0

None

BibTeX

@article{Andrews_2002, title={Drosophila Sec10 is Required for Hormone Secretion but not General Exocytosis or Neurotransmission}, volume={3}, ISSN={1600-0854}, url={http://dx.doi.org/10.1034/j.1600-0854.2002.31206.x}, DOI={10.1034/j.1600-0854.2002.31206.x}, number={12}, journal={Traffic}, publisher={Wiley}, author={Andrews, Hillary K. and Zhang, Yong Q. and Trotta, Nick and Broadie, Kendal}, year={2002}, month=dec, pages={906–921} }

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  "journal-issue": {
    "issue": "12",
    "published-print": {
      "date-parts": [
        [
          2002,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1034/j.1600-0854.2002.31206.x"
  ],
  "URL": "http://dx.doi.org/10.1034/j.1600-0854.2002.31206.x",
  "relation": {},
  "ISSN": [
    "1398-9219",
    "1600-0854"
  ],
  "subject": [],
  "container-title-short": "Traffic",
  "published": {
    "date-parts": [
      [
        2002,
        12
      ]
    ]
  }
}