DOI: 10.1034/j.1600-0781.2002.00765.x. Cyclosporin‐A suppresses p53‐dependent repair DNA synthesis and apoptosis following ultraviolet‐B irradiation

Cyclosporin‐A suppresses p53‐dependent repair DNA synthesis and apoptosis following ultraviolet‐B irradiation

10.1034/j.1600-0781.2002.00765.x

Crossref journal-article

Wiley

Photodermatology, Photoimmunology & Photomedicine (311)

Abstract

Background: The combination of cyclosporin‐A (CS‐A) and ultraviolet‐B (UV‐B) irradiation is not recommended in the treatment of psoriasis, because risks of UV‐B‐induced skin cancer are increased. The recommendation, however, has not well been confirmed by basic researches.Purpose: In this study, we investigated the effects of CS‐A on UV‐B‐induced repair DNA synthesis, apoptosis and p53 expression.Methods: Following the short‐term administration of CS‐A (5 and 50 mg/kg/day) or vehicle (V) alone, female BALB/c mice, 8–10 weeks old, were treated with UV‐B irradiation (100 and 500 mJ2 cm) or tape stripping (TS). After the treatment, the effects of CS‐A on the increased rate of epidermal DNA synthesis were examined by using 5′‐bromodeoxyuridine (BrdU) pulse‐labelling techniques. In separate experiments, the effects of CS‐A on the number of sunburn cells, nick‐end labelling + cells and p53 + cells were examined 24 h after UV‐B irradiation.Results: Cyclosporin‐A significantly suppressed the UV‐B‐induced increase in BrdU uptake, which occurs to repair DNA damage, while there were no significant effects on the stripping (S)‐induced increase or the rate of normal epidermal proliferation, which is not associated with any DNA injuries. The number of sunburn cells, nick‐end labelling + cells and p53 + cells was significantly reduced by pretreatment with CS‐A.Conclusion: Cyclosporin‐A interferes with the self‐ protective mechanisms involved in both repair and apoptotic removal of UV‐B‐induced DNA damage. The loss of p53 expression is responsible for the effects of CS‐A.

Bibliography

Sugie, N., Fujii, N., & Danno, K. (2002). CyclosporinâA suppresses p53âdependent repair DNA synthesis and apoptosis following ultravioletâB irradiation. Photodermatology, Photoimmunology & Photomedicine, 18(4), 163â168. Portico.

Authors 3

N. Sugie (first)
N. Fujii (additional)
K. Danno (additional)

References 20 Referenced 26

{'key': 'e_1_2_5_2_2', 'first-page': '587', 'volume-title': 'Psoriasis', 'author': 'Roenigk HH', 'year': '1998'} / Psoriasis by Roenigk HH (1998)
10.1001/archderm.1989.01670160093019 / Arch Dermatol / Risk assessment of PUVA and cyclosporine by Stern RS (1989)
10.1016/S0140-6736(97)80015-3
10.1016/S0041-1345(96)00153-4
10.1073/pnas.93.1.274
{'key': 'e_1_2_5_7_2', 'first-page': '136', 'article-title': 'Sunlight and sunburn in human skin cancer: p53, apoptosis, and tumor promotion', 'volume': '1', 'author': 'Brash DE', 'year': '1996', 'journal-title': 'J\xa0Invest Dermatol Proc'} / J Invest Dermatol Proc / Sunlight and sunburn in human skin cancer: p53, apoptosis, and tumor promotion by Brash DE (1996)
10.1016/S0190-9622(83)70160-X
10.1111/j.1600-0714.1995.tb01196.x
10.1111/j.1751-1097.1970.tb06038.x
10.1016/0923-1811(92)90019-8
10.1111/j.1751-1097.1981.tb08987.x
10.1046/j.1365-2133.1998.02306.x
10.1111/j.1751-1097.1987.tb07401.x
10.1046/j.1523-1747.1999.00474.x
10.1111/1523-1747.ep12263506
10.1038/372773a0
10.1046/j.1523-1747.1998.00090.x
10.1097/00007890-198709000-00021
{'key': 'e_1_2_5_20_2', 'first-page': '526', 'article-title': 'Increased ultraviolet radiation‐induced skin cancers in cyclosporine‐treated mice', 'volume': '19', 'author': 'Nelson EW', 'year': '1987', 'journal-title': 'Transplant Proc'} / Transplant Proc / Increased ultraviolet radiation‐induced skin cancers in cyclosporine‐treated mice by Nelson EW (1987)
10.1097/00007890-198708000-00022

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:35 p.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 4:06 a.m.)
Indexed	1 year ago (Aug. 11, 2024, 9:49 a.m.)
Issued	23 years ago (Aug. 1, 2002)
Published	23 years ago (Aug. 1, 2002)
Published Online	22 years, 10 months ago (Oct. 4, 2002)
Published Print	23 years ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Sugie_2002, title={Cyclosporin‐A suppresses p53‐dependent repair DNA synthesis and apoptosis following ultraviolet‐B irradiation}, volume={18}, ISSN={1600-0781}, url={http://dx.doi.org/10.1034/j.1600-0781.2002.00765.x}, DOI={10.1034/j.1600-0781.2002.00765.x}, number={4}, journal={Photodermatology, Photoimmunology & Photomedicine}, publisher={Wiley}, author={Sugie, N. and Fujii, N. and Danno, K.}, year={2002}, month=aug, pages={163–168} }

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