DOI: 10.1034/j.1399-3054.1998.1040205.x. Purification and characterization of strongly chitin‐binding chitinases from salicylic acid‐treated leek (<i>Allium porrum</i>)

Purification and characterization of strongly chitin‐binding chitinases from salicylic acid‐treated leek (Allium porrum)

10.1034/j.1399-3054.1998.1040205.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

Six chitinases (EC 3.2.1.14) were purified from salicylate‐treated leek (Allium porrum L.). They all strongly bind to chitin and can roughly be divided into two groups. One group has blocked N‐termini, is completely inhibited by 1 mM AgNO3, has a relatively narrow pH optimum, a temperature optimum of 40°C and cannot degrade the tetramer of chitin. The other group has unblocked N‐termini showing homology to the chitin‐binding lectin WGA and is therefore considered as class I chitinases. This group is only moderately inhibited by 1 mM AgNO3 (30%), has a relatively broad pH optimum, has a higher temperature optimum (50 to 60°C) and can degrade the tetramer of chitin to dimers. Furthermore, all isoforms have molecular masses around 34 kDa as estimated by SDS‐PAGE. They have isoelectric points ranging from 4 to 8 and no detectable lysozyme activity. Two isoforms investigated in more detail differ in their antifungal potential.

Bibliography

Vergauwen, R., Van Leuven, F., & Van Laere, A. (1998). Purification and characterization of strongly chitinâbinding chitinases from salicylic acidâtreated leek (Allium porrum). Physiologia Plantarum, 104(2), 175â182. Portico.

Authors 3

Rudy Vergauwen (first)
Fred Van Leuven (additional)
André Van Laere (additional)

References 0 Referenced 26

None

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:23 p.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 3:44 a.m.)
Indexed	1 year, 2 months ago (June 13, 2024, 1:14 p.m.)
Issued	26 years, 11 months ago (Oct. 1, 1998)
Published	26 years, 11 months ago (Oct. 1, 1998)
Published Online	23 years, 7 months ago (Jan. 17, 2002)
Published Print	26 years, 11 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{Vergauwen_1998, title={Purification and characterization of strongly chitin‐binding chitinases from salicylic acid‐treated leek (Allium porrum)}, volume={104}, ISSN={1399-3054}, url={http://dx.doi.org/10.1034/j.1399-3054.1998.1040205.x}, DOI={10.1034/j.1399-3054.1998.1040205.x}, number={2}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Vergauwen, Rudy and Van Leuven, Fred and Van Laere, André}, year={1998}, month=oct, pages={175–182} }

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