DOI: 10.1034/j.1398-9995.2001.00115.x. Calreticulin is an interleukin‐3‐sensitive calcium‐binding protein in human basophil leukocytes

Calreticulin is an interleukin‐3‐sensitive calcium‐binding protein in human basophil leukocytes

10.1034/j.1398-9995.2001.00115.x

Crossref journal-article

Wiley

Allergy (311)

Abstract

Background: IL‐3 enhances basophil histamine release upon stimulation with any known secretagogue. The molecular mechanism behind this regulation is not known, although some observations suggest that IL‐3 modulates the calcium part of the signal transduction mechanism. The inhibitory action of glucocorticoids on basophils can be reversed by stimulation with IL‐3. Methods: Calcium‐binding proteins in the basophil cell line KU812 were identified by two‐dimensional gel electrophoresis, Calcium‐overlay assay, N‐terminal sequence analysis, and mass spectometry. The presence of the same proteins in purified human basophil leukocytes was established by comigration of KU812 and human basophil proteins on the two‐dimensional gels. The expression of the calcium‐binding proteins in the absence and presence of IL‐3 and/or anti‐IgE was determined by densitometric measurement of the spots on the two‐dimensional gels. Results: Calreticulin was identified on the two‐dimensional gel of KU812 proteins. A protein with exactly the same migration pattern was found on the gels of proteins from purified human basophils. Immunoblotting with a specific anti‐human calreticulin antibody confirmed that this protein was calreticulin. Subsequent analysis showed that the expression of calreticulin in the basophils is upregulated twofold upon stimulation with rhIL‐3, even in doses below those needed for enhancement of histamine release. Conclusions: The expression of calreticulin in human basophil leukocytes is regulated by IL‐3. Calreticulin is known to modulate IP3‐dependent Ca2+ influx in different cell systems, and calreticulin overexpression inhibits steroid‐induced transcriptional activation. Therefore, modulation of calreticulin expression may be one mechanism by which IL‐3 exerts its effects on human basophils.

Bibliography

Lyngholm, M. J., Nielsen, H. V., Holm, M., SchiÃ¸tz, P. O., & Johnsen, A. H. (2001). Calreticulin is an interleukinâ3âsensitive calciumâbinding protein in human basophil leukocytes. Allergy, 56(1), 21â28. Portico.

Authors 5

M. J. Lyngholm (first)
H. V. Nielsen (additional)
M. Holm (additional)
P. O. Schiøtz (additional)
A. H. Johnsen (additional)

References 36 Referenced 10

10.1172/JCI114047
10.4049/jimmunol.148.7.2223 / J Immunol / Histamine‐containing cells obtained from the nose hours after antigen challenge have functional and phenotypic characteristics of basophils by Iliopoulos O (1992)
10.1182/blood.V79.6.1491.1491 / Blood / Effects of in vivo administration of interleukin‐2 (IL‐2) and IL‐ 4, alone and in combination, on ex vivo human basophil histamine release by White MV (1992)
10.4049/jimmunol.141.11.3958 / J Immunol / Modulation of human basophil histamine release by hemopoietic growth factors by Hirai K (1988)
10.1084/jem.177.3.605
10.1002/eji.1830210217
10.1006/clin.1993.1067
10.1002/jcp.1041450111
10.1159/000319280
10.4049/jimmunol.150.3.980 / J Immunol / Biphasic Ca2+ responses in human basophils. Evidence that the initial transient elevation associated with the mobilization of intracellular calcium is an insufficient signal for degranulation by MacGlashan DJ (1993)
10.1016/0092-8674(95)90473-5
10.1016/0022-1759(93)90069-J
10.1007/BF01973840
{'key': 'e_1_2_6_15_2', 'first-page': '222', 'volume-title': 'Cell biology. A laboratory handbook', 'author': 'Celis JE', 'year': '1994'} / Cell biology. A laboratory handbook by Celis JE (1994)
{'key': 'e_1_2_6_16_2', 'first-page': '309', 'volume-title': 'Cell biology. A laboratory handbook', 'author': 'Hoffman HJ', 'year': '1994'} / Cell biology. A laboratory handbook by Hoffman HJ (1994)
10.1038/379466a0
10.1016/0968-0004(94)90153-8
10.1111/j.1398-9995.1998.tb03950.x
10.1182/blood.V90.1.372
10.1111/j.1398-9995.1998.tb03891.x
10.1002/jlb.49.1.29
10.4049/jimmunol.151.11.6358 / J Immunol / IL‐3 alters free arachidonic acid generation in C5a‐stimulated human basophils by MacGlashan DWJ (1993)
10.1016/S0091-6749(96)70196-3
10.1016/0091-6749(90)90046-7
10.1083/jcb.130.4.847
10.1074/jbc.271.16.9332
10.1074/jbc.271.46.29436
10.1016/S0143-4160(97)90075-0
10.1083/jcb.144.5.857
10.1016/S0021-9258(19)61952-6 / J Biol Chem / Decreasing calreticulin expression lowers the Ca2+ response to bradykinin and increases sensitivity to ionomycin in NG‐108–15 cells by Liu N (1994)
10.1038/386843a0
10.1093/nar/23.16.3268
10.1038/367476a0
10.1006/excr.1996.3408
10.1038/367480a0
10.4049/jimmunol.143.4.1310 / J Immunol / Regulation of human basophil mediator release by cytokines. I. Interaction with antiinflammatory steroids by Schleimer RP (1989)

Dates

Type	When
Created	22 years, 5 months ago (March 10, 2003, 8:45 p.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 3:49 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 3, 2024, 9:51 p.m.)
Issued	24 years, 7 months ago (Jan. 1, 2001)
Published	24 years, 7 months ago (Jan. 1, 2001)
Published Online	16 years, 11 months ago (Sept. 23, 2008)
Published Print	24 years, 7 months ago (Jan. 1, 2001)

Funders 0

None

BibTeX

@article{Lyngholm_2001, title={Calreticulin is an interleukin‐3‐sensitive calcium‐binding protein in human basophil leukocytes}, volume={56}, ISSN={1398-9995}, url={http://dx.doi.org/10.1034/j.1398-9995.2001.00115.x}, DOI={10.1034/j.1398-9995.2001.00115.x}, number={1}, journal={Allergy}, publisher={Wiley}, author={Lyngholm, M. J. and Nielsen, H. V. and Holm, M. and Schiøtz, P. O. and Johnsen, A. H.}, year={2001}, month=jan, pages={21–28} }

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