DOI: 10.1021/ja993254x. NMR Evidence for an Unusually Low N1 p<i>K</i><sub>a</sub> for Uracil Bound to Uracil DNA Glycosylase: Implications for Catalysis

NMR Evidence for an Unusually Low N1 pKa for Uracil Bound to Uracil DNA Glycosylase: Implications for Catalysis

10.1021/ja993254x

Crossref journal-article

American Chemical Society (ACS)

Journal of the American Chemical Society (316)

Bibliography

Drohat, A. C., & Stivers, J. T. (2000). NMR Evidence for an Unusually Low N1 pKa for Uracil Bound to Uracil DNA Glycosylase:â Implications for Catalysis. Journal of the American Chemical Society, 122(8), 1840â1841.

Authors 2

Alexander C. Drohat (first)
James T. Stivers (additional)

References 24 Referenced 72

10.1021/cr980321h / Chem. Rev. by David S. S. (1998)
10.1021/ja00704a008 / J. Am. Chem. Soc. by Zoltewicz J. A. (1970)
10.1021/ja00756a009 / J. J Am Chem Soc by Garrett E. R. (1972)
{'key': 'ja993254xb00003/ja993254xb00003_1', 'first-page': '29', 'volume': '11', 'author': 'Shapiro R.', 'year': '1972', 'journal-title': 'Biochemistry'} / Biochemistry by Shapiro R. (1972)
10.1093/emboj/17.17.5214 / EMBO. J. by Parikh S. S. (1998)
10.1002/(SICI)1097-0134(19990401)35:1<13::AID-PROT2>3.0.CO;2-2 / Proteins by Xiao G. (1999)
10.1021/bi9818669 / Biochemistry by Stivers J. T. (1999)
{'key': 'ja993254xb00006/ja993254xb00006_1', 'first-page': '11875', 'volume': '38', 'author': 'Drohat A. C.', 'year': '1999', 'journal-title': 'Biochemistry'} / Biochemistry by Drohat A. C. (1999)
{'key': 'ja993254xb00006/ja993254xb00006_2', 'first-page': '11886', 'volume': '38', 'author': 'Drohat A. C.', 'year': '1999', 'journal-title': 'Biochemistry'} / Biochemistry by Drohat A. C. (1999)
10.1021/ja00077a062 / J. Am. Chem. Soc. by Gerlt J. A. (1993)
{'key': 'ja993254xb00009/ja993254xb00009_1', 'first-page': '9', 'volume': '93', 'author': 'Shan S. O.', 'year': '1996', 'journal-title': 'Proc. Natl. Acad. Sci. U.S.A.'} / Proc. Natl. Acad. Sci. U.S.A. by Shan S. O. (1996)
{'key': 'ja993254xb00010/ja993254xb00010_1', 'first-page': '9220', 'volume': '38', 'author': 'Luo N.', 'year': '1999', 'journal-title': 'Biochemistry'} / Biochemistry by Luo N. (1999)
10.1021/bi982986j / Biochemistry by Shroyer M. J. (1999)
{'key': 'ja993254xb00012/ja993254xb00012_1', 'volume-title': 'Enzyme Structure and Mechanism', 'author': 'Fersht A. R.', 'year': '1985'} / Enzyme Structure and Mechanism by Fersht A. R. (1985)
10.1021/ja9640051 / J. Am. Chem. Soc. by Legault P. (1997)
10.1016/S0079-6565(97)00023-X / Prog. Nucl. Magn. Reson. Spectrosc. by Wijmenga S. S. (1998)
{'key': 'ja993254xb00017/ja993254xb00017_1', 'volume-title': 'P. J. Nuclear Magnetic Resonance', 'author': 'From', 'year': '1995'} / P. J. Nuclear Magnetic Resonance by From (1995)
{'key': 'ja993254xb00018/ja993254xb00018_1', 'first-page': '10', 'volume': '8', 'author': 'Shapiro R.', 'year': '1969', 'journal-title': 'Biochemistry'} / Biochemistry by Shapiro R. (1969)
10.1021/ja01534a041 / J. Am. Chem. Soc. by Bruice T. C. (1958)
{'key': 'ja993254xb00021/ja993254xb00021_1', 'volume': '253', 'author': 'Ka 0.', 'year': '1991', 'journal-title': 'J. Science'} / J. Science by Ka 0. (1991)
{'key': 'ja993254xb00022/ja993254xb00022_1', 'first-page': '245', 'volume': '308', 'author': 'Mildvan A. S.', 'year': '1999', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. by Mildvan A. S. (1999)
10.1007/BF00211777 / J. Biomol. NMR by Wishart D. S. (1995)
10.1006/jmrb.1995.1109 / J. Magn. Reson. B by Mori S. (1995)
Leatherbarrow, R. J.GraFit 4.0; Erithacus Software Ltd. Staines, U.K., 1998.

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 1:56 a.m.)
Deposited	3 years, 10 months ago (Oct. 17, 2021, 7:15 p.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:24 a.m.)
Issued	25 years, 6 months ago (Feb. 15, 2000)
Published	25 years, 6 months ago (Feb. 15, 2000)
Published Online	25 years, 6 months ago (Feb. 15, 2000)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Drohat_2000, title={NMR Evidence for an Unusually Low N1 pKa for Uracil Bound to Uracil DNA Glycosylase: Implications for Catalysis}, volume={122}, ISSN={1520-5126}, url={http://dx.doi.org/10.1021/ja993254x}, DOI={10.1021/ja993254x}, number={8}, journal={Journal of the American Chemical Society}, publisher={American Chemical Society (ACS)}, author={Drohat, Alexander C. and Stivers, James T.}, year={2000}, month=feb, pages={1840–1841} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        24
      ]
    ],
    "date-time": "2025-07-24T11:24:54Z",
    "timestamp": 1753356294177
  },
  "reference-count": 24,
  "publisher": "American Chemical Society (ACS)",
  "issue": "8",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        3,
        1
      ]
    ]
  },
  "DOI": "10.1021/ja993254x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T05:56:09Z",
    "timestamp": 1027662969000
  },
  "page": "1840-1841",
  "source": "Crossref",
  "is-referenced-by-count": 72,
  "title": "NMR Evidence for an Unusually Low N1 p<i>K</i><sub>a</sub> for Uracil Bound to Uracil DNA Glycosylase:\u2009 Implications for Catalysis",
  "prefix": "10.1021",
  "volume": "122",
  "author": [
    {
      "given": "Alexander C.",
      "family": "Drohat",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute for Standards and Technology 9600 Gudelsky Drive, Rockville, Maryland 20850"
        }
      ]
    },
    {
      "given": "James T.",
      "family": "Stivers",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Center for Advanced Research in Biotechnology of the University of Maryland Biotechnology Institute and the National Institute for Standards and Technology 9600 Gudelsky Drive, Rockville, Maryland 20850"
        }
      ]
    }
  ],
  "member": "316",
  "published-online": {
    "date-parts": [
      [
        2000,
        2,
        15
      ]
    ]
  },
  "reference": [
    {
      "key": "ja993254xb00001/ja993254xb00001_1",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1021/cr980321h",
      "volume": "98",
      "author": "David S. S.",
      "year": "1998",
      "journal-title": "Chem. Rev."
    },
    {
      "key": "ja993254xb00002/ja993254xb00002_1",
      "doi-asserted-by": "crossref",
      "first-page": "50",
      "DOI": "10.1021/ja00704a008",
      "volume": "92",
      "author": "Zoltewicz J. A.",
      "year": "1970",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "ja993254xb00002/ja993254xb00002_2",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1021/ja00756a009",
      "volume": "94",
      "author": "Garrett E. R.",
      "year": "1972",
      "journal-title": "J. J Am Chem Soc"
    },
    {
      "key": "ja993254xb00003/ja993254xb00003_1",
      "first-page": "29",
      "volume": "11",
      "author": "Shapiro R.",
      "year": "1972",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00004/ja993254xb00004_1",
      "doi-asserted-by": "crossref",
      "first-page": "26",
      "DOI": "10.1093/emboj/17.17.5214",
      "volume": "17",
      "author": "Parikh S. S.",
      "year": "1998",
      "journal-title": "EMBO. J."
    },
    {
      "key": "ja993254xb00004/ja993254xb00004_2",
      "doi-asserted-by": "crossref",
      "first-page": "24",
      "DOI": "10.1002/(SICI)1097-0134(19990401)35:1<13::AID-PROT2>3.0.CO;2-2",
      "volume": "35",
      "author": "Xiao G.",
      "year": "1999",
      "journal-title": "Proteins"
    },
    {
      "key": "ja993254xb00005/ja993254xb00005_1",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1021/bi9818669",
      "volume": "38",
      "author": "Stivers J. T.",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00006/ja993254xb00006_1",
      "first-page": "11875",
      "volume": "38",
      "author": "Drohat A. C.",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00006/ja993254xb00006_2",
      "first-page": "11886",
      "volume": "38",
      "author": "Drohat A. C.",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00008/ja993254xb00008_1",
      "doi-asserted-by": "crossref",
      "first-page": "11568",
      "DOI": "10.1021/ja00077a062",
      "volume": "115",
      "author": "Gerlt J. A.",
      "year": "1993",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "ja993254xb00009/ja993254xb00009_1",
      "first-page": "9",
      "volume": "93",
      "author": "Shan S. O.",
      "year": "1996",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "ja993254xb00010/ja993254xb00010_1",
      "first-page": "9220",
      "volume": "38",
      "author": "Luo N.",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00011/ja993254xb00011_1",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1021/bi982986j",
      "volume": "38",
      "author": "Shroyer M. J.",
      "year": "1999",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00012/ja993254xb00012_1",
      "volume-title": "Enzyme Structure and Mechanism",
      "author": "Fersht A. R.",
      "year": "1985"
    },
    {
      "key": "ja993254xb00013/ja993254xb00013_1",
      "doi-asserted-by": "crossref",
      "first-page": "6628",
      "DOI": "10.1021/ja9640051",
      "volume": "119",
      "author": "Legault P.",
      "year": "1997",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "ja993254xb00014/ja993254xb00014_1",
      "doi-asserted-by": "crossref",
      "first-page": "387",
      "DOI": "10.1016/S0079-6565(97)00023-X",
      "volume": "32",
      "author": "Wijmenga S. S.",
      "year": "1998",
      "journal-title": "Prog. Nucl. Magn. Reson. Spectrosc."
    },
    {
      "key": "ja993254xb00017/ja993254xb00017_1",
      "volume-title": "P. J. Nuclear Magnetic Resonance",
      "author": "From",
      "year": "1995"
    },
    {
      "key": "ja993254xb00018/ja993254xb00018_1",
      "first-page": "10",
      "volume": "8",
      "author": "Shapiro R.",
      "year": "1969",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ja993254xb00020/ja993254xb00020_1",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1021/ja01534a041",
      "volume": "80",
      "author": "Bruice T. C.",
      "year": "1958",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "ja993254xb00021/ja993254xb00021_1",
      "volume": "253",
      "author": "Ka 0.",
      "year": "1991",
      "journal-title": "J. Science"
    },
    {
      "key": "ja993254xb00022/ja993254xb00022_1",
      "first-page": "245",
      "volume": "308",
      "author": "Mildvan A. S.",
      "year": "1999",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "ja993254xb00023/ja993254xb00023_1",
      "doi-asserted-by": "crossref",
      "first-page": "40",
      "DOI": "10.1007/BF00211777",
      "volume": "6",
      "author": "Wishart D. S.",
      "year": "1995",
      "journal-title": "J. Biomol. NMR"
    },
    {
      "key": "ja993254xb00024/ja993254xb00024_1",
      "doi-asserted-by": "crossref",
      "first-page": "8",
      "DOI": "10.1006/jmrb.1995.1109",
      "volume": "108",
      "author": "Mori S.",
      "year": "1995",
      "journal-title": "J. Magn. Reson. B"
    },
    {
      "key": "ja993254xb00025/ja993254xb00025_1",
      "unstructured": "Leatherbarrow, R. J.GraFit 4.0; Erithacus Software Ltd.\u2009 Staines, U.K., 1998."
    }
  ],
  "container-title": "Journal of the American Chemical Society",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pubs.acs.org/doi/pdf/10.1021/ja993254x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        10,
        17
      ]
    ],
    "date-time": "2021-10-17T23:15:39Z",
    "timestamp": 1634512539000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pubs.acs.org/doi/10.1021/ja993254x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        2,
        15
      ]
    ]
  },
  "references-count": 24,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          2000,
          3,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1021/ja993254x"
  ],
  "URL": "http://dx.doi.org/10.1021/ja993254x",
  "relation": {},
  "ISSN": [
    "0002-7863",
    "1520-5126"
  ],
  "subject": [],
  "container-title-short": "J. Am. Chem. Soc.",
  "published": {
    "date-parts": [
      [
        2000,
        2,
        15
      ]
    ]
  }
}