DOI: 10.1021/bi9605954. High Frequency (139.5 GHz) Electron Paramagnetic Resonance Spectroscopy of the GTP Form of p21 <i>ras</i> with Selective <sup>17</sup>O Labeling of Threonine

High Frequency (139.5 GHz) Electron Paramagnetic Resonance Spectroscopy of the GTP Form of p21 ras with Selective 17O Labeling of Threonine

10.1021/bi9605954

Crossref journal-article

American Chemical Society (ACS)

Biochemistry (316)

Bibliography

Halkides, C. J., Bellew, B. F., Gerfen, G. J., Farrar, C. T., Carter, P. H., Ruo, B., Evans, D. A., Griffin, R. G., & Singel, D. J. (1996). High Frequency (139.5 GHz) Electron Paramagnetic Resonance Spectroscopy of the GTP Form of p21 ras with Selective 17O Labeling of Threonine. Biochemistry, 35(37), 12194â12200.

Authors 9

Christopher J. Halkides (first)
Brendan F. Bellew (additional)
Gary J. Gerfen (additional)
Christian T. Farrar (additional)
Percy H. Carter (additional)
Bernice Ruo (additional)
David A. Evans (additional)
Robert G. Griffin (additional)
David J. Singel (additional)

References 52 Referenced 20

{'key': 'atypb1/atypb2', 'volume-title': 'Electron Paramagnetic Resonance of Transition Ions', 'author': 'Abragam A.', 'year': '1970'} / Electron Paramagnetic Resonance of Transition Ions by Abragam A. (1970)
10.1006/jmra.1995.9975 / J. Magn. Reson., Ser. A by Becerra L. R. (1995)
{'key': 'atypb5/atypb6', 'volume-title': 'Biochemistry 35, 12186−12193', 'author': 'Bellew B. F.', 'year': '1996'} / Biochemistry 35, 12186−12193 by Bellew B. F. (1996)
{'key': 'atypb7/atypb8', 'volume-title': 'Nature 348, 125−132', 'author': 'Bourne H. R.', 'year': '1990'} / Nature 348, 125−132 by Bourne H. R. (1990)
10.1016/S0020-1693(00)87542-8 / Inorg. Chim. Acta by Bruckner S. (1993)
10.1021/ja00234a011 / J. Am. Chem. Soc. by Carrell C. J. (1988)
{'key': 'atypb13/atypb14', 'volume': '29', 'author': 'Carrell H. L.', 'year': '1973', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. by Carrell H. L. (1973)
{'key': 'atypb15/atypb16', 'volume': '36', 'author': 'Ciunik Z.', 'year': '1980', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. by Ciunik Z. (1980)
{'key': 'atypb17/atypb18', 'volume': '43', 'author': 'Clegg W.', 'year': '1987', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. by Clegg W. (1987)
{'key': 'atypb19/atypb20', 'volume': '29', 'author': 'DeBeer R.', 'year': '1973', 'journal-title': 'Acta Crystallogr.'} / Acta Crystallogr. by DeBeer R. (1973)
10.1021/ja00281a049 / J. Am. Chem. Soc. by Evans D. A. (1986)
10.1021/ja00356a055 / J. Am. Chem. Soc. by Evans D. A. (1983)
{'key': 'atypb25/atypb26', 'volume-title': 'Mol. Cell. Biol. 11, 4822−4829', 'author': 'Farnsworth C. L.', 'year': '1991'} / Mol. Cell. Biol. 11, 4822−4829 by Farnsworth C. L. (1991)
10.1111/j.1432-1033.1987.tb10540.x / Eur. J. Biochem. by Feuerstein J. (1987)
{'key': 'atypb29/atypb30', 'volume-title': 'Gaussian', 'author': 'Frisch M. J.', 'year': '1992'} / Gaussian by Frisch M. J. (1992)
{'key': 'atypb31/atypb32', 'volume-title': 'Cell. Biol. 12', 'author': 'Gideon P.', 'year': '1992'} / Cell. Biol. 12 by Gideon P. (1992)
Glotfelty, H. W. (1978) Ph.D. Thesis, University of Kansas.
10.1016/0022-2860(73)87015-2 / J. Mol. Struct. by Glusker J. P. (1973)
{'key': 'atypb37/atypb38', 'volume-title': 'Biochemistry 33, 4019−4035', 'author': 'Halkides C. J.', 'year': '1994'} / Biochemistry 33, 4019−4035 by Halkides C. J. (1994)
10.1063/1.448975 / J. Chem. Phys. by Hay P. J. (1985)
{'key': 'atypb41/atypb42', 'volume': '82', 'author': 'Hay P. J.', 'year': '1985', 'journal-title': 'J. Chem. Phys.'} / J. Chem. Phys. by Hay P. J. (1985)
{'key': 'atypb43/atypb44', 'first-page': '13', 'volume': '96', 'author': 'Hyde J. S.', 'year': '1992', 'journal-title': 'J. Magn. Reson.'} / J. Magn. Reson. by Hyde J. S. (1992)
10.1016/S0021-9258(18)54022-9 / J. Biol. Chem. by John J. (1993)
10.1111/j.1432-1033.1984.tb08234.x / Eur. J. Biochem. by Kalbitzer H. R. (1984)
10.1021/ic50155a010 / Inorg. Chem. by Karipides A. (1976)
{'key': 'atypb51/atypb52', 'volume-title': 'Biochemistry 31, 9608−9611', 'author': 'Latwesen D. G.', 'year': '1992'} / Biochemistry 31, 9608−9611 by Latwesen D. G. (1992)
{'key': 'atypb53/atypb54', 'volume-title': 'Bull. Soc. Chim. Belg. 92, 257−262', 'author': 'Lenstra A. T. H.', 'year': '1983'} / Bull. Soc. Chim. Belg. 92, 257−262 by Lenstra A. T. H. (1983)
{'key': 'atypb55/atypb56', 'volume-title': 'Biochemistry 26, 2243−2250', 'author': 'Lodato D. T.', 'year': '1987'} / Biochemistry 26, 2243−2250 by Lodato D. T. (1987)
{'key': 'atypb57/atypb58', 'volume-title': 'Trends Biochem. Sci. 18, 250−254', 'author': 'Marshall M. S.', 'year': '1993'} / Trends Biochem. Sci. 18, 250−254 by Marshall M. S. (1993)
{'key': 'atypb59/atypb60', 'volume-title': 'Science 247, 939−945', 'author': 'Milburn M.', 'year': '1990'} / Science 247, 939−945 by Milburn M. (1990)
{'key': 'atypb61/atypb62', 'volume-title': 'Biochemistry 31, 10208−10216', 'author': 'Miller A.-F.', 'year': '1992'} / Biochemistry 31, 10208−10216 by Miller A.-F. (1992)
{'key': 'atypb63/atypb64', 'volume-title': 'Biochemistry 32, 7367−7376', 'author': 'Miller A.-F.', 'year': '1993'} / Biochemistry 32, 7367−7376 by Miller A.-F. (1993)
{'key': 'atypb65/atypb66', 'volume-title': 'Science 260, 1658−1661', 'author': 'Moodie S. A.', 'year': '1993'} / Science 260, 1658−1661 by Moodie S. A. (1993)
{'key': 'atypb67/atypb68', 'volume-title': 'Nature 375, 554−560', 'author': 'Nassar N.', 'year': '1995'} / Nature 375, 554−560 by Nassar N. (1995)
{'key': 'atypb69/atypb70', 'volume': '9', 'author': 'Pai E. F.', 'year': '1990', 'journal-title': 'EMBO J.'} / EMBO J. by Pai E. F. (1990)
{'key': 'atypb71/atypb72', 'volume-title': 'Biochemistry 19, 5472−5480', 'author': 'Reed G. H.', 'year': '1980'} / Biochemistry 19, 5472−5480 by Reed G. H. (1980)
{'key': 'atypb73/atypb74', 'first-page': '142', 'volume': '6', 'author': 'Reed G. H.', 'year': '1984', 'journal-title': 'Biol. Magn. Reson.'} / Biol. Magn. Reson. by Reed G. H. (1984)
{'key': 'atypb75/atypb76', 'volume-title': 'Biochemistry 24, 7827−7833', 'author': 'Sabat M.', 'year': '1985'} / Biochemistry 24, 7827−7833 by Sabat M. (1985)
10.1021/jo00798a070 / J. Org. Chem. by Sawyer C. B. (1972)
{'key': 'atypb79/atypb80', 'volume-title': 'Oncogene 9, 2153−2157', 'author': 'Shirouzu M.', 'year': '1994'} / Oncogene 9, 2153−2157 by Shirouzu M. (1994)
{'key': 'atypb81/atypb82', 'volume': '72', 'author': 'Shoemaker D. P.', 'year': '1950', 'journal-title': 'J. Am. Chem. Soc.'} / J. Am. Chem. Soc. by Shoemaker D. P. (1950)
{'key': 'atypb83/atypb84', 'volume-title': 'Biochemistry 34, 3470−3477', 'author': 'Sung Y.-S.', 'year': '1995'} / Biochemistry 34, 3470−3477 by Sung Y.-S. (1995)
{'key': 'atypb85/atypb86', 'volume-title': 'Biochemistry 32, 7799−7810', 'author': 'Tan X.', 'year': '1993'} / Biochemistry 32, 7799−7810 by Tan X. (1993)
{'key': 'atypb87/atypb88', 'volume': '102', 'author': 'Tan X.', 'year': '1995', 'journal-title': 'J. Chem. Phys.'} / J. Chem. Phys. by Tan X. (1995)
10.1016/0022-2836(91)90753-S / J. Mol. Biol. by Tong L. (1991)
{'key': 'atypb91/atypb92', 'volume-title': 'Z. Kristallogr. 173, 193−198', 'author': 'Vasic P.', 'year': '1985'} / Z. Kristallogr. 173, 193−198 by Vasic P. (1985)
10.1038/335093a0 / Nature by Vogel U. S. (1988)
10.1063/1.448800 / J. Chem. Phys. by Wadt W. R. (1985)
Weber, A. E. (1987) Ph.D. Thesis, Harvard University.
{'key': 'atypb99/atypb100', 'volume-title': 'Cell 80, 533−541', 'author': 'White M. A.', 'year': '1995'} / Cell 80, 533−541 by White M. A. (1995)
{'key': 'bi9605954b00001/bi9605954b00001_1', 'volume-title': 'product of the ras genes', 'author': 'Abbreviations'} / product of the ras genes by Abbreviations
An alternative explanation for the small value of the experimentally determined hyperfine coupling constant is that the incorporation of isotopically labeled threonine is lower than expected. However, the enrichment is determined by GC/MS analysis of protein hydrolyzate in both the ESEEM work and the present EPR study (Figure 1). The 89% enrichment of the protein, relative to the starting amino acid, falls in the range previously observed for the enrichment of threonine residues, 75−89% (Halkideset al., 1994). Thus, the level of incorporation of labeled threonine is consistently high and is explicitly taken into each analysis.

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 1:21 a.m.)
Deposited	3 years, 10 months ago (Oct. 14, 2021, 1:15 a.m.)
Indexed	1 year, 11 months ago (Oct. 6, 2023, 8:02 a.m.)
Issued	29 years, 8 months ago (Jan. 1, 1996)
Published	29 years, 8 months ago (Jan. 1, 1996)
Published Online	28 years, 11 months ago (Sept. 17, 1996)
Published Print	29 years, 8 months ago (Jan. 1, 1996)

Funders 0

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BibTeX

@article{Halkides_1996, title={High Frequency (139.5 GHz) Electron Paramagnetic Resonance Spectroscopy of the GTP Form of p21 ras with Selective 17O Labeling of Threonine}, volume={35}, ISSN={1520-4995}, url={http://dx.doi.org/10.1021/bi9605954}, DOI={10.1021/bi9605954}, number={37}, journal={Biochemistry}, publisher={American Chemical Society (ACS)}, author={Halkides, Christopher J. and Bellew, Brendan F. and Gerfen, Gary J. and Farrar, Christian T. and Carter, Percy H. and Ruo, Bernice and Evans, David A. and Griffin, Robert G. and Singel, David J.}, year={1996}, month=jan, pages={12194–12200} }

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    {
      "key": "atypb1/atypb2",
      "volume-title": "Electron Paramagnetic Resonance of Transition Ions",
      "author": "Abragam A.",
      "year": "1970"
    },
    {
      "key": "atypb3/atypb4",
      "doi-asserted-by": "crossref",
      "first-page": "40",
      "DOI": "10.1006/jmra.1995.9975",
      "volume": "117",
      "author": "Becerra L. R.",
      "year": "1995",
      "journal-title": "J. Magn. Reson., Ser. A"
    },
    {
      "key": "atypb5/atypb6",
      "volume-title": "Biochemistry 35, 12186\u221212193",
      "author": "Bellew B. F.",
      "year": "1996"
    },
    {
      "key": "atypb7/atypb8",
      "volume-title": "Nature 348, 125\u2212132",
      "author": "Bourne H. R.",
      "year": "1990"
    },
    {
      "key": "atypb9/atypb10",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/S0020-1693(00)87542-8",
      "volume": "214",
      "author": "Bruckner S.",
      "year": "1993",
      "journal-title": "Inorg. Chim. Acta"
    },
    {
      "key": "atypb11/atypb12",
      "doi-asserted-by": "crossref",
      "DOI": "10.1021/ja00234a011",
      "volume": "110",
      "author": "Carrell C. J.",
      "year": "1988",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "atypb13/atypb14",
      "volume": "29",
      "author": "Carrell H. L.",
      "year": "1973",
      "journal-title": "Acta Crystallogr."
    },
    {
      "key": "atypb15/atypb16",
      "volume": "36",
      "author": "Ciunik Z.",
      "year": "1980",
      "journal-title": "Acta Crystallogr."
    },
    {
      "key": "atypb17/atypb18",
      "volume": "43",
      "author": "Clegg W.",
      "year": "1987",
      "journal-title": "Acta Crystallogr."
    },
    {
      "key": "atypb19/atypb20",
      "volume": "29",
      "author": "DeBeer R.",
      "year": "1973",
      "journal-title": "Acta Crystallogr."
    },
    {
      "key": "atypb21/atypb22",
      "doi-asserted-by": "crossref",
      "first-page": "6757",
      "DOI": "10.1021/ja00281a049",
      "volume": "108",
      "author": "Evans D. A.",
      "year": "1986",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "atypb23/atypb24",
      "doi-asserted-by": "crossref",
      "first-page": "5946",
      "DOI": "10.1021/ja00356a055",
      "volume": "105",
      "author": "Evans D. A.",
      "year": "1983",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "atypb25/atypb26",
      "volume-title": "Mol. Cell. Biol. 11, 4822\u22124829",
      "author": "Farnsworth C. L.",
      "year": "1991"
    },
    {
      "key": "atypb27/atypb28",
      "doi-asserted-by": "crossref",
      "first-page": "55",
      "DOI": "10.1111/j.1432-1033.1987.tb10540.x",
      "volume": "162",
      "author": "Feuerstein J.",
      "year": "1987",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "atypb29/atypb30",
      "volume-title": "Gaussian",
      "author": "Frisch M. J.",
      "year": "1992"
    },
    {
      "key": "atypb31/atypb32",
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      "year": "1992"
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    {
      "key": "atypb33/atypb34",
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      "key": "atypb35/atypb36",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/0022-2860(73)87015-2",
      "volume": "15",
      "author": "Glusker J. P.",
      "year": "1973",
      "journal-title": "J. Mol. Struct."
    },
    {
      "key": "atypb37/atypb38",
      "volume-title": "Biochemistry 33, 4019\u22124035",
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      "year": "1994"
    },
    {
      "key": "atypb39/atypb40",
      "doi-asserted-by": "crossref",
      "first-page": "299",
      "DOI": "10.1063/1.448975",
      "volume": "82",
      "author": "Hay P. J.",
      "year": "1985",
      "journal-title": "J. Chem. Phys."
    },
    {
      "key": "atypb41/atypb42",
      "volume": "82",
      "author": "Hay P. J.",
      "year": "1985",
      "journal-title": "J. Chem. Phys."
    },
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      "key": "atypb43/atypb44",
      "first-page": "13",
      "volume": "96",
      "author": "Hyde J. S.",
      "year": "1992",
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    },
    {
      "key": "atypb45/atypb46",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/S0021-9258(18)54022-9",
      "volume": "268",
      "author": "John J.",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "atypb47/atypb48",
      "doi-asserted-by": "crossref",
      "DOI": "10.1111/j.1432-1033.1984.tb08234.x",
      "volume": "141",
      "author": "Kalbitzer H. R.",
      "year": "1984",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "atypb49/atypb50",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1021/ic50155a010",
      "volume": "15",
      "author": "Karipides A.",
      "year": "1976",
      "journal-title": "Inorg. Chem."
    },
    {
      "key": "atypb51/atypb52",
      "volume-title": "Biochemistry 31, 9608\u22129611",
      "author": "Latwesen D. G.",
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    },
    {
      "key": "atypb53/atypb54",
      "volume-title": "Bull. Soc. Chim. Belg. 92, 257\u2212262",
      "author": "Lenstra A. T. H.",
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    },
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      "key": "atypb55/atypb56",
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      "key": "atypb57/atypb58",
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      "year": "1993"
    },
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      "key": "atypb59/atypb60",
      "volume-title": "Science 247, 939\u2212945",
      "author": "Milburn M.",
      "year": "1990"
    },
    {
      "key": "atypb61/atypb62",
      "volume-title": "Biochemistry 31, 10208\u221210216",
      "author": "Miller A.-F.",
      "year": "1992"
    },
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      "key": "atypb63/atypb64",
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      "key": "atypb65/atypb66",
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    {
      "key": "atypb67/atypb68",
      "volume-title": "Nature 375, 554\u2212560",
      "author": "Nassar N.",
      "year": "1995"
    },
    {
      "key": "atypb69/atypb70",
      "volume": "9",
      "author": "Pai E. F.",
      "year": "1990",
      "journal-title": "EMBO J."
    },
    {
      "key": "atypb71/atypb72",
      "volume-title": "Biochemistry 19, 5472\u22125480",
      "author": "Reed G. H.",
      "year": "1980"
    },
    {
      "key": "atypb73/atypb74",
      "first-page": "142",
      "volume": "6",
      "author": "Reed G. H.",
      "year": "1984",
      "journal-title": "Biol. Magn. Reson."
    },
    {
      "key": "atypb75/atypb76",
      "volume-title": "Biochemistry 24, 7827\u22127833",
      "author": "Sabat M.",
      "year": "1985"
    },
    {
      "key": "atypb77/atypb78",
      "doi-asserted-by": "crossref",
      "first-page": "4225",
      "DOI": "10.1021/jo00798a070",
      "volume": "37",
      "author": "Sawyer C. B.",
      "year": "1972",
      "journal-title": "J. Org. Chem."
    },
    {
      "key": "atypb79/atypb80",
      "volume-title": "Oncogene 9, 2153\u22122157",
      "author": "Shirouzu M.",
      "year": "1994"
    },
    {
      "key": "atypb81/atypb82",
      "volume": "72",
      "author": "Shoemaker D. P.",
      "year": "1950",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "atypb83/atypb84",
      "volume-title": "Biochemistry 34, 3470\u22123477",
      "author": "Sung Y.-S.",
      "year": "1995"
    },
    {
      "key": "atypb85/atypb86",
      "volume-title": "Biochemistry 32, 7799\u22127810",
      "author": "Tan X.",
      "year": "1993"
    },
    {
      "key": "atypb87/atypb88",
      "volume": "102",
      "author": "Tan X.",
      "year": "1995",
      "journal-title": "J. Chem. Phys."
    },
    {
      "key": "atypb89/atypb90",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/0022-2836(91)90753-S",
      "volume": "217",
      "author": "Tong L.",
      "year": "1991",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "atypb91/atypb92",
      "volume-title": "Z. Kristallogr. 173, 193\u2212198",
      "author": "Vasic P.",
      "year": "1985"
    },
    {
      "key": "atypb93/atypb94",
      "doi-asserted-by": "crossref",
      "first-page": "93",
      "DOI": "10.1038/335093a0",
      "volume": "335",
      "author": "Vogel U. S.",
      "year": "1988",
      "journal-title": "Nature"
    },
    {
      "key": "atypb95/atypb96",
      "doi-asserted-by": "crossref",
      "first-page": "284",
      "DOI": "10.1063/1.448800",
      "volume": "82",
      "author": "Wadt W. R.",
      "year": "1985",
      "journal-title": "J. Chem. Phys."
    },
    {
      "key": "atypb97/atypb98",
      "unstructured": "Weber, A. E. (1987) Ph.D. Thesis, Harvard University."
    },
    {
      "key": "atypb99/atypb100",
      "volume-title": "Cell 80, 533\u2212541",
      "author": "White M. A.",
      "year": "1995"
    },
    {
      "key": "bi9605954b00001/bi9605954b00001_1",
      "volume-title": "product of the ras genes",
      "author": "Abbreviations"
    },
    {
      "key": "bi9605954b00003/bi9605954b00003_1",
      "unstructured": "An alternative explanation for the small value of the experimentally determined hyperfine coupling constant is that the incorporation of isotopically labeled threonine is lower than expected. However, the enrichment is determined by GC/MS analysis of protein hydrolyzate in both the ESEEM work and the present EPR study (Figure 1). The 89% enrichment of the protein, relative to the starting amino acid, falls in the range previously observed for the enrichment of threonine residues, 75\u221289% (Halkideset al., 1994). Thus, the level of incorporation of labeled threonine is consistently high and is explicitly taken into each analysis."
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