DOI: 10.1021/bc049843w. Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEGylated Proteins

Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEGylated Proteins

10.1021/bc049843w

Crossref journal-article

American Chemical Society (ACS)

Bioconjugate Chemistry (316)

Bibliography

Fee, C. J., & Van Alstine, J. M. (2004). Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEGylated Proteins. Bioconjugate Chemistry, 15(6), 1304â1313.

Authors 2

Conan J. Fee (first)
James M. Van Alstine (additional)

References 37 Referenced 145

{'key': 'bc049843wb00001/bc049843wb00001_1', 'volume-title': 'Biopolymer Mixtures', 'author': 'Fisher D.', 'year': '1995'} / Biopolymer Mixtures by Fisher D. (1995)
{'key': 'bc049843wb00002/bc049843wb00002_1', 'volume-title': 'Strategies for the preparation and characterization of poly(ethylene glycol) (PEG) conjugated pharmaceutical proteins. Polym. Prepr. 38 (1), 565−66', 'author': 'Fung W.-J.', 'year': '1997'} / Strategies for the preparation and characterization of poly(ethylene glycol) (PEG) conjugated pharmaceutical proteins. Polym. Prepr. 38 (1), 565−66 by Fung W.-J. (1997)
{'issue': '11', 'key': 'bc049843wb00003/bc049843wb00003_1', 'volume': '252', 'author': 'Abuchowski A.', 'year': '1977', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. by Abuchowski A. (1977)
10.1007/978-1-4899-0703-5 / Poly(ethylene glycol) Chemistry: Biotechnical and Biomedical Applications by Harris J. M. (1992)
{'key': 'bc049843wb00005/bc049843wb00005_1', 'volume-title': 's, (1997) Poly(ethylene glycol):\u2009 Chemistry and Biological Applications', 'author': 'Harris J. M.'} / s, (1997) Poly(ethylene glycol): Chemistry and Biological Applications by Harris J. M.
10.1016/0022-1759(92)90139-K / J. Immunol. Methods by Cunningham-Rundles C. (1992)
{'issue': '1', 'key': 'bc049843wb00007/bc049843wb00007_1', 'first-page': '18', 'volume': '68', 'author': 'Francis G. E.', 'year': '1998', 'journal-title': 'Int. J.Hematol.'} / Int. J.Hematol. by Francis G. E. (1998)
{'key': 'bc049843wb00008/bc049843wb00008_1', 'volume-title': 'Controlled Drug Delivery:\u2009 Designing technologies for the future', 'author': 'Gombotz W. R.', 'year': '2000'} / Controlled Drug Delivery: Designing technologies for the future by Gombotz W. R. (2000)
{'key': 'bc049843wb00009/bc049843wb00009_1', 'volume-title': 'Factors with impact on the success of protein and virus PEGylation. Polym. Prepr. 41 (1), 1012−3', 'author': 'Fisher D.', 'year': '2000'} / Factors with impact on the success of protein and virus PEGylation. Polym. Prepr. 41 (1), 1012−3 by Fisher D. (2000)
Bullock, J., Chowdhury, S., and Johnston, D. (1996) Characterization of poly(ethylene glycol) modified superoxide dismutase: comparison of capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.Ann. Chem. 68, 3258−64. (10.1021/ac960240q)
Kendrick, B. S., Kerwin, B. A., Chang, B. S., and Philo, J. S. (2001) Online size-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein−protein or protein−ligand association states.Anal. Biochem. 299(2), 136−46. (10.1006/abio.2001.5411)
Hellberg, U., Ivarsson, J.P., and Johansson, B.L. (1996) Characteristics of Superdex prep grade media for gel filtration of proteins and peptides.Process Biochem. 31(2), 163−72. (10.1016/0032-9592(95)00044-5)
{'key': 'bc049843wb00013/bc049843wb00013_1', 'volume-title': 'Size exclusion reaction chromatography (SERC):\u2009 a new technique for protein PEGylation. Biotechnol. Bioeng. 82 (2), 200−06', 'author': 'Fee C. J.', 'year': '2003'} / Size exclusion reaction chromatography (SERC): a new technique for protein PEGylation. Biotechnol. Bioeng. 82 (2), 200−06 by Fee C. J. (2003)
{'key': 'bc049843wb00014/bc049843wb00014_1', 'volume-title': 'Analysis of poly(ethylene glycol) modified superoxide dismutase by chromatographic, electrophoretic, light scattering, chemical and enzymatic methods. Chem. Pharm. Bull. 36 (8), 3079−91', 'author': 'McGoff P.', 'year': '1988'} / Analysis of poly(ethylene glycol) modified superoxide dismutase by chromatographic, electrophoretic, light scattering, chemical and enzymatic methods. Chem. Pharm. Bull. 36 (8), 3079−91 by McGoff P. (1988)
{'key': 'bc049843wb00015/bc049843wb00015_1', 'volume-title': 'Surface modification of horseradish peroxidase with poly(ethylene glycol)s of various molecular masses. Biotechnol. Appl. Biochem. 17 (1), 115−30', 'author': 'Fortier G.', 'year': '1993'} / Surface modification of horseradish peroxidase with poly(ethylene glycol)s of various molecular masses. Biotechnol. Appl. Biochem. 17 (1), 115−30 by Fortier G. (1993)
{'key': 'bc049843wb00016/bc049843wb00016_1', 'volume-title': 'Enhancement of pH-stability of a low molecular mass endoglucanase from Fusarium oxysporum by protein PEGylation. Carbohydr. Res. 314 (1−2), 95−99', 'author': 'Christakopoulos P.', 'year': '1998'} / Enhancement of pH-stability of a low molecular mass endoglucanase from Fusarium oxysporum by protein PEGylation. Carbohydr. Res. 314 (1−2), 95−99 by Christakopoulos P. (1998)
10.1016/S0142-9612(00)00193-9 / Peptide and protein PEGylation: a review of problems and solutions. Biomaterials 22, 405−17 by Veronese F. M. (2001)
{'key': 'bc049843wb00018/bc049843wb00018_1', 'author': 'Benoit H.', 'year': '1966', 'journal-title': 'J. Chim. Phys. 63 (11−12), 1507−14.'} / J. Chim. Phys. 63 (11−12), 1507−14. by Benoit H. (1966)
10.1016/0021-9673(91)85014-7 / J. Chromatogr. by Kunitani M. (1991)
{'key': 'bc049843wb00020/bc049843wb00020_1', 'volume-title': 'Protein Purification (Janson, J.-C., and Rydén', 'author': 'Hagel L.', 'year': '1998', 'edition': '2'} / Protein Purification (Janson, J.-C., and Rydén by Hagel L. (1998)
10.1016/S0021-9673(00)88914-1 / J. Chromatogr. by Kuga S. (1981)
{'key': 'bc049843wb00022/bc049843wb00022_1', 'first-page': '93', 'volume': '930', 'author': 'Hunter A. K.', 'year': '2001', 'journal-title': 'J. Chromtogr. A'} / J. Chromtogr. A by Hunter A. K. (2001)
{'key': 'bc049843wb00023/bc049843wb00023_1', 'volume-title': 'Poly(ethylene glycol) Chemistry and Biological Applications (Harris, J. M., and Zalipsky, S.', 'author': 'Karlström G.', 'year': '1997'} / Poly(ethylene glycol) Chemistry and Biological Applications (Harris, J. M., and Zalipsky, S. by Karlström G. (1997)
{'key': 'bc049843wb00024/bc049843wb00024_1', 'volume-title': 'Aqueous solution properties oligo and poly(ethylene oxide) by light scattering and intrinsic viscosity. Polymer 38, 2885−91', 'author': 'Seigou K.', 'year': '1997'} / Aqueous solution properties oligo and poly(ethylene oxide) by light scattering and intrinsic viscosity. Polymer 38, 2885−91 by Seigou K. (1997)
{'key': 'bc049843wb00025/bc049843wb00025_1', 'volume': '280', 'author': 'Yoko M.', 'year': '2002', 'journal-title': 'J. Colloid Polym. Sci.'} / J. Colloid Polym. Sci. by Yoko M. (2002)
10.1021/j100269a014 / J. Phys. Chem. by Andersson M. (1985)
{'key': 'bc049843wb00027/bc049843wb00027_1', 'volume': '122', 'author': 'Smith G. D.', 'year': '2000', 'journal-title': 'J. Am. Chem. Soc.'} / J. Am. Chem. Soc. by Smith G. D. (2000)
{'key': 'bc049843wb00028/bc049843wb00028_1', 'volume-title': 'Polymers at interfaces', 'author': 'Fleer G. J.', 'year': '1993'} / Polymers at interfaces by Fleer G. J. (1993)
{'key': 'bc049843wb00029/bc049843wb00029_1', 'volume-title': 'Protein-induced changes in poly(ethylene glycol) brushes:\u2009 molecular weight and temperature difference. Langmuir 17, 7628−36', 'author': 'Efremova N. V.', 'year': '2001'} / Protein-induced changes in poly(ethylene glycol) brushes: molecular weight and temperature difference. Langmuir 17, 7628−36 by Efremova N. V. (2001)
{'key': 'bc049843wb00030/bc049843wb00030_1', 'volume-title': 'Stability of poly(ethylene -glycol) graft coatings. Langmuir 14, 2722−29', 'author': 'Emoto K.', 'year': '1998'} / Stability of poly(ethylene -glycol) graft coatings. Langmuir 14, 2722−29 by Emoto K. (1998)
10.1016/S0378-4347(96)00522-1 / J. Chromatogr. B by Delgado C. (1997)
10.1006/jcis.1998.5513 / J. Colloid Interface Sci. by Malmsten M. (1998)
{'key': 'bc049843wb00033/bc049843wb00033_1', 'volume-title': 'Mechanism of protein-poly(ethylene glycol) interaction from a diffusive point of view. Macromolecules 35, 1389−98', 'author': 'Vergara A.', 'year': '2002'} / Mechanism of protein-poly(ethylene glycol) interaction from a diffusive point of view. Macromolecules 35, 1389−98 by Vergara A. (2002)
{'key': 'bc049843wb00034/bc049843wb00034_1', 'volume-title': 'Partition of cell particles and macromolecules', 'author': 'Albertsson P.', 'year': '1986'} / Partition of cell particles and macromolecules by Albertsson P. (1986)
10.1016/0003-2697(86)90431-8 / Partitioning in aqueous two-phase systems: an overview. Anal. Chem. 155 (2), 215−42 by Walter H. (1986)
{'key': 'bc049843wb00036/bc049843wb00036_1', 'volume-title': 'PEGylations of a lysine-deficient TNF-a with full bioactivity. Nature Biotechnol. 21, 546−52.', 'author': 'Yamamoto Y.', 'year': '2002'} / PEGylations of a lysine-deficient TNF-a with full bioactivity. Nature Biotechnol. 21, 546−52. by Yamamoto Y. (2002)
{'key': 'bc049843wb00037/bc049843wb00037_1', 'author': 'Na D. H.', 'year': '2002', 'journal-title': 'J. Chromtogr. B 754, (259−63).'} / J. Chromtogr. B 754, (259−63). by Na D. H. (2002)

Dates

Type	When
Created	20 years, 9 months ago (Nov. 17, 2004, 12:31 a.m.)
Deposited	2 years, 5 months ago (March 13, 2023, 9:03 p.m.)
Indexed	2 weeks ago (Aug. 21, 2025, 12:58 p.m.)
Issued	20 years, 10 months ago (Oct. 30, 2004)
Published	20 years, 10 months ago (Oct. 30, 2004)
Published Online	20 years, 10 months ago (Oct. 30, 2004)
Published Print	20 years, 10 months ago (Nov. 1, 2004)

Funders 0

None

BibTeX

@article{Fee_2004, title={Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEGylated Proteins}, volume={15}, ISSN={1520-4812}, url={http://dx.doi.org/10.1021/bc049843w}, DOI={10.1021/bc049843w}, number={6}, journal={Bioconjugate Chemistry}, publisher={American Chemical Society (ACS)}, author={Fee, Conan J. and Van Alstine, James M.}, year={2004}, month=oct, pages={1304–1313} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T16:58:58Z",
    "timestamp": 1755795538600
  },
  "reference-count": 37,
  "publisher": "American Chemical Society (ACS)",
  "issue": "6",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        11,
        1
      ]
    ]
  },
  "DOI": "10.1021/bc049843w",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        11,
        17
      ]
    ],
    "date-time": "2004-11-17T05:31:32Z",
    "timestamp": 1100669492000
  },
  "page": "1304-1313",
  "source": "Crossref",
  "is-referenced-by-count": 145,
  "title": "Prediction of the Viscosity Radius and the Size Exclusion Chromatography Behavior of PEGylated Proteins",
  "prefix": "10.1021",
  "volume": "15",
  "author": [
    {
      "given": "Conan J.",
      "family": "Fee",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Materials and Process Engineering, University of Waikato, Private Bag 3105, Hamilton 2020, New Zealand, and Protein Separations, General Electric Healthcare, Bjorkgatan 30, Uppsala 75184, Sweden"
        }
      ]
    },
    {
      "given": "James M.",
      "family": "Van Alstine",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Materials and Process Engineering, University of Waikato, Private Bag 3105, Hamilton 2020, New Zealand, and Protein Separations, General Electric Healthcare, Bjorkgatan 30, Uppsala 75184, Sweden"
        }
      ]
    }
  ],
  "member": "316",
  "published-online": {
    "date-parts": [
      [
        2004,
        10,
        30
      ]
    ]
  },
  "reference": [
    {
      "key": "bc049843wb00001/bc049843wb00001_1",
      "volume-title": "Biopolymer Mixtures",
      "author": "Fisher D.",
      "year": "1995"
    },
    {
      "key": "bc049843wb00002/bc049843wb00002_1",
      "volume-title": "Strategies for the preparation and characterization of poly(ethylene glycol) (PEG) conjugated pharmaceutical proteins. Polym. Prepr. 38 (1), 565\u221266",
      "author": "Fung W.-J.",
      "year": "1997"
    },
    {
      "issue": "11",
      "key": "bc049843wb00003/bc049843wb00003_1",
      "volume": "252",
      "author": "Abuchowski A.",
      "year": "1977",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "bc049843wb00004/bc049843wb00004_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1007/978-1-4899-0703-5",
      "volume-title": "Poly(ethylene glycol) Chemistry:\u2009 Biotechnical and Biomedical Applications",
      "author": "Harris J. M.",
      "year": "1992"
    },
    {
      "key": "bc049843wb00005/bc049843wb00005_1",
      "volume-title": "s, (1997) Poly(ethylene glycol):\u2009 Chemistry and Biological Applications",
      "author": "Harris J. M."
    },
    {
      "issue": "2",
      "key": "bc049843wb00006/bc049843wb00006_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/0022-1759(92)90139-K",
      "volume": "152",
      "author": "Cunningham-Rundles C.",
      "year": "1992",
      "journal-title": "J. Immunol. Methods"
    },
    {
      "issue": "1",
      "key": "bc049843wb00007/bc049843wb00007_1",
      "first-page": "18",
      "volume": "68",
      "author": "Francis G. E.",
      "year": "1998",
      "journal-title": "Int. J.Hematol."
    },
    {
      "key": "bc049843wb00008/bc049843wb00008_1",
      "volume-title": "Controlled Drug Delivery:\u2009 Designing technologies for the future",
      "author": "Gombotz W. R.",
      "year": "2000"
    },
    {
      "key": "bc049843wb00009/bc049843wb00009_1",
      "volume-title": "Factors with impact on the success of protein and virus PEGylation. Polym. Prepr. 41 (1), 1012\u22123",
      "author": "Fisher D.",
      "year": "2000"
    },
    {
      "key": "bc049843wb00010/bc049843wb00010_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Bullock, J., Chowdhury, S., and Johnston, D. (1996) Characterization of poly(ethylene glycol) modified superoxide dismutase:\u2009 comparison of capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.Ann. Chem. 68, 3258\u221264.",
      "DOI": "10.1021/ac960240q"
    },
    {
      "key": "bc049843wb00011/bc049843wb00011_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Kendrick, B. S., Kerwin, B. A., Chang, B. S., and Philo, J. S. (2001) Online size-exclusion high-performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and protein\u2212protein or protein\u2212ligand association states.Anal. Biochem. 299(2), 136\u221246.",
      "DOI": "10.1006/abio.2001.5411"
    },
    {
      "key": "bc049843wb00012/bc049843wb00012_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Hellberg, U., Ivarsson, J.P., and Johansson, B.L. (1996) Characteristics of Superdex prep grade media for gel filtration of proteins and peptides.Process Biochem. 31(2), 163\u221272.",
      "DOI": "10.1016/0032-9592(95)00044-5"
    },
    {
      "key": "bc049843wb00013/bc049843wb00013_1",
      "volume-title": "Size exclusion reaction chromatography (SERC):\u2009 a new technique for protein PEGylation. Biotechnol. Bioeng. 82 (2), 200\u221206",
      "author": "Fee C. J.",
      "year": "2003"
    },
    {
      "key": "bc049843wb00014/bc049843wb00014_1",
      "volume-title": "Analysis of poly(ethylene glycol) modified superoxide dismutase by chromatographic, electrophoretic, light scattering, chemical and enzymatic methods. Chem. Pharm. Bull. 36 (8), 3079\u221291",
      "author": "McGoff P.",
      "year": "1988"
    },
    {
      "key": "bc049843wb00015/bc049843wb00015_1",
      "volume-title": "Surface modification of horseradish peroxidase with poly(ethylene glycol)s of various molecular masses. Biotechnol. Appl. Biochem. 17 (1), 115\u221230",
      "author": "Fortier G.",
      "year": "1993"
    },
    {
      "key": "bc049843wb00016/bc049843wb00016_1",
      "volume-title": "Enhancement of pH-stability of a low molecular mass endoglucanase from Fusarium oxysporum by protein PEGylation. Carbohydr. Res. 314 (1\u22122), 95\u221299",
      "author": "Christakopoulos P.",
      "year": "1998"
    },
    {
      "key": "bc049843wb00017/bc049843wb00017_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/S0142-9612(00)00193-9",
      "volume-title": "Peptide and protein PEGylation:\u2009 a review of problems and solutions. Biomaterials 22, 405\u221217",
      "author": "Veronese F. M.",
      "year": "2001"
    },
    {
      "key": "bc049843wb00018/bc049843wb00018_1",
      "author": "Benoit H.",
      "year": "1966",
      "journal-title": "J. Chim. Phys. 63 (11\u221212), 1507\u221214."
    },
    {
      "key": "bc049843wb00019/bc049843wb00019_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/0021-9673(91)85014-7",
      "volume": "588",
      "author": "Kunitani M.",
      "year": "1991",
      "journal-title": "J. Chromatogr."
    },
    {
      "key": "bc049843wb00020/bc049843wb00020_1",
      "volume-title": "Protein Purification (Janson, J.-C., and Ryd\u00e9n",
      "author": "Hagel L.",
      "year": "1998",
      "edition": "2"
    },
    {
      "issue": "3",
      "key": "bc049843wb00021/bc049843wb00021_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/S0021-9673(00)88914-1",
      "volume": "206",
      "author": "Kuga S.",
      "year": "1981",
      "journal-title": "J. Chromatogr."
    },
    {
      "key": "bc049843wb00022/bc049843wb00022_1",
      "first-page": "93",
      "volume": "930",
      "author": "Hunter A. K.",
      "year": "2001",
      "journal-title": "J. Chromtogr. A"
    },
    {
      "key": "bc049843wb00023/bc049843wb00023_1",
      "volume-title": "Poly(ethylene glycol) Chemistry and Biological Applications (Harris, J. M., and Zalipsky, S.",
      "author": "Karlstr\u00f6m G.",
      "year": "1997"
    },
    {
      "key": "bc049843wb00024/bc049843wb00024_1",
      "volume-title": "Aqueous solution properties oligo and poly(ethylene oxide) by light scattering and intrinsic viscosity. Polymer 38, 2885\u221291",
      "author": "Seigou K.",
      "year": "1997"
    },
    {
      "key": "bc049843wb00025/bc049843wb00025_1",
      "volume": "280",
      "author": "Yoko M.",
      "year": "2002",
      "journal-title": "J. Colloid Polym. Sci."
    },
    {
      "issue": "23",
      "key": "bc049843wb00026/bc049843wb00026_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1021/j100269a014",
      "volume": "89",
      "author": "Andersson M.",
      "year": "1985",
      "journal-title": "J. Phys. Chem."
    },
    {
      "key": "bc049843wb00027/bc049843wb00027_1",
      "volume": "122",
      "author": "Smith G. D.",
      "year": "2000",
      "journal-title": "J. Am. Chem. Soc."
    },
    {
      "key": "bc049843wb00028/bc049843wb00028_1",
      "volume-title": "Polymers at interfaces",
      "author": "Fleer G. J.",
      "year": "1993"
    },
    {
      "key": "bc049843wb00029/bc049843wb00029_1",
      "volume-title": "Protein-induced changes in poly(ethylene glycol) brushes:\u2009 molecular weight and temperature difference. Langmuir 17, 7628\u221236",
      "author": "Efremova N. V.",
      "year": "2001"
    },
    {
      "key": "bc049843wb00030/bc049843wb00030_1",
      "volume-title": "Stability of poly(ethylene -glycol) graft coatings. Langmuir 14, 2722\u221229",
      "author": "Emoto K.",
      "year": "1998"
    },
    {
      "issue": "2",
      "key": "bc049843wb00031/bc049843wb00031_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/S0378-4347(96)00522-1",
      "volume": "692",
      "author": "Delgado C.",
      "year": "1997",
      "journal-title": "J. Chromatogr. B"
    },
    {
      "issue": "2",
      "key": "bc049843wb00032/bc049843wb00032_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1006/jcis.1998.5513",
      "volume": "202",
      "author": "Malmsten M.",
      "year": "1998",
      "journal-title": "J. Colloid Interface Sci."
    },
    {
      "key": "bc049843wb00033/bc049843wb00033_1",
      "volume-title": "Mechanism of protein-poly(ethylene glycol) interaction from a diffusive point of view. Macromolecules 35, 1389\u221298",
      "author": "Vergara A.",
      "year": "2002"
    },
    {
      "key": "bc049843wb00034/bc049843wb00034_1",
      "volume-title": "Partition of cell particles and macromolecules",
      "author": "Albertsson P.",
      "year": "1986"
    },
    {
      "key": "bc049843wb00035/bc049843wb00035_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1016/0003-2697(86)90431-8",
      "volume-title": "Partitioning in aqueous two-phase systems:\u2009 an overview. Anal. Chem. 155 (2), 215\u221242",
      "author": "Walter H.",
      "year": "1986"
    },
    {
      "key": "bc049843wb00036/bc049843wb00036_1",
      "volume-title": "PEGylations of a lysine-deficient TNF-a with full bioactivity. Nature Biotechnol. 21, 546\u221252.",
      "author": "Yamamoto Y.",
      "year": "2002"
    },
    {
      "key": "bc049843wb00037/bc049843wb00037_1",
      "author": "Na D. H.",
      "year": "2002",
      "journal-title": "J. Chromtogr. B 754, (259\u221263)."
    }
  ],
  "container-title": "Bioconjugate Chemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pubs.acs.org/doi/pdf/10.1021/bc049843w",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        3,
        14
      ]
    ],
    "date-time": "2023-03-14T01:03:19Z",
    "timestamp": 1678755799000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pubs.acs.org/doi/10.1021/bc049843w"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        10,
        30
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          2004,
          11,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1021/bc049843w"
  ],
  "URL": "http://dx.doi.org/10.1021/bc049843w",
  "relation": {},
  "ISSN": [
    "1043-1802",
    "1520-4812"
  ],
  "subject": [],
  "container-title-short": "Bioconjugate Chem.",
  "published": {
    "date-parts": [
      [
        2004,
        10,
        30
      ]
    ]
  }
}