DOI: 10.1017/s1551929500054432. A Versatile and Affordable Plunge Freezing Instrument for Preparing Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM)

A Versatile and Affordable Plunge Freezing Instrument for Preparing Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM)

10.1017/s1551929500054432

Crossref journal-article

Oxford University Press (OUP)

Microscopy Today (286)

Abstract

AbstractCryoEM is a powerful tool in the arsenal of structural biologists and soft polymer chemists. Hydrated specimens require a preservation method that will counteract the effects of the electron beam and the high vacuum environment of the electron microscope. Classical specimen preparation techniques using chemical fixatives are not able to capture the native structure of the once hydrated specimen perfectly. In contrast to classical methods for preserving specimens for electron microscopy, rapid freezing of radiation-sensitive specimens such as dispersed biological macromolecular assemblies, 2D crystals, and colloids allows the structural details of the specimen to be captured in their essentially native state to near atomic resolution.

Bibliography

Melanson, L. (2009). A Versatile and Affordable Plunge Freezing Instrument for Preparing Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM). Microscopy Today, 17(2), 14â17.

Authors 1

Linda Melanson (first)

References 11 Referenced 2

10.1111/j.1365-2818.1987.tb02822.x / Journal of Microscopy / The construction and operation of a simple and inexpensive slam freezing device for electron microscopy by Allison (1987)
10.1111/j.1365-2818.1984.tb02519.x / Journal of Microscopy / The relative efficiency of cryogenic fluids used in the rapid quench-cooling of cryogenic samples by Bald (1984)
{'key': '2023010802411450500_ref003', 'volume-title': 'Quantitative Cryofixation', 'author': 'Bald', 'year': '1987'} / Quantitative Cryofixation by Bald (1987)
10.1111/j.1365-2818.1994.tb03505.x / Journal of Microscopy / Vitrification of aqueous suspensions from a controlled environment for electron microsocopy: an improved plunge-cooling device by Battersby (1994)
{'key': '2023010802411450500_ref005', 'first-page': '107', 'author': 'Dubochet', 'year': '1982', 'journal-title': 'Advances in optical and electron microscopy'} / Advances in optical and electron microscopy by Dubochet (1982)
10.1017/S0033583500004297 / Quarterly Review of Biophysics / Cryo-electron microscopy of vitrified specimens by Dubochet (1988)
10.1007/978-1-4899-2302-8 / Low Temperature Microscopy and Analysis by Echlin (1992)
{'issue': '2', 'key': '2023010802411450500_ref008', 'first-page': '112', 'article-title': 'A new method of preparation of a self-perforated micro plastic grid and its application', 'volume': '14', 'author': 'Fukami', 'year': '1965', 'journal-title': 'J Electron Microscopy (Japan)'} / J Electron Microscopy (Japan) / A new method of preparation of a self-perforated micro plastic grid and its application by Fukami (1965)
10.1093/oso/9780195088717.001.0001 / Electron Crystallography of Biological Macromolecules by Glaser (2007)
{'key': '2023010802411450500_ref010', 'author': 'Melanson', 'year': '2008'} by Melanson (2008)
10.1007/978-3-642-72815-0 / Cryotechniques in biological electron microscopy by Steinbrecht (1987)

Dates

Type	When
Created	7 years, 2 months ago (May 22, 2018, 10:20 a.m.)
Deposited	1 year, 1 month ago (July 6, 2024, 6:40 p.m.)
Indexed	1 year, 1 month ago (July 6, 2024, 7:40 p.m.)
Issued	16 years, 5 months ago (March 1, 2009)
Published	16 years, 5 months ago (March 1, 2009)
Published Online	7 years, 5 months ago (March 14, 2018)
Published Print	16 years, 5 months ago (March 1, 2009)

Funders 0

None

BibTeX

@article{Melanson_2009, title={A Versatile and Affordable Plunge Freezing Instrument for Preparing Frozen Hydrated Specimens for Cryo Transmission Electron Microscopy (CryoEM)}, volume={17}, ISSN={1551-9295}, url={http://dx.doi.org/10.1017/s1551929500054432}, DOI={10.1017/s1551929500054432}, number={2}, journal={Microscopy Today}, publisher={Oxford University Press (OUP)}, author={Melanson, Linda}, year={2009}, month=mar, pages={14–17} }

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